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ORIGINAL RESEARCH article
Front. Cell. Infect. Microbiol.
Sec. Extra-intestinal Microbiome
Volume 14 - 2024 |
doi: 10.3389/fcimb.2024.1320831
Pathogen Spectrum and Microbiome in Lower Respiratory Tract of Patients with Different Pulmonary Diseases Based on Metagenomic Next-Generation Sequencing
Provisionally accepted- 1 Fujian Provincial Hospital, Fuzhou, Fujian Province, China
- 2 Matridx Biotechnology Co., Ltd, Hangzhou, Zhejiang, China
- 3 Institute of Biophysics, Chinese Academy of Sciences (CAS), Beijing, Beijing Municipality, China
- 4 Department of Critical Care Medicine, Fujian Provincial Hospital, Fuzhou, China
The homeostasis of the microbiome in lower respiratory tract is crucial in sustaining normal physiological functions of the lung. Different pulmonary diseases display varying degrees of microbiome imbalance; however, the specific variability and clinical significance of their microbiomes remain largely unexplored. In this study, we delineated the pathogen spectrum and commensal microorganisms in the lower respiratory tract of various pulmonary diseases using metagenomic sequencing. We analyzed the disparities and commonalities of the microbial features and examined their correlation with disease characteristics. We observed distinct pathogen profiles and a diversity in lower airway microbiome in patients diagnosed with cancer, interstitial lung disease, bronchiectasis, common pneumonia, Nontuberculous mycobacteria (NTM) pneumonia, and severe pneumonia. This study illustrates the utility of Metagenomic Next-generation Sequencing (mNGS) in identifying pathogens and analyzing the lower respiratory microbiome, which is important for understanding the microbiological aspect of pulmonary diseases and essential for their early and precise diagnosis.
Keywords: pulmonary disease, Lower respiratory tract, MNGs, pathogen, microbiome
Received: 24 Oct 2023; Accepted: 08 Oct 2024.
Copyright: © 2024 Hong, Lin, Zhang, Yi, Li, Yang, Du, Cao, Wu, Ren, Yao and Xie. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Baosong Xie, Department of Critical Care Medicine, Fujian Provincial Hospital, Fuzhou, China
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