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ORIGINAL RESEARCH article

Front. Cell. Infect. Microbiol.
Sec. Extra-intestinal Microbiome
Volume 14 - 2024 | doi: 10.3389/fcimb.2024.1320831

Pathogen Spectrum and Microbiome in Lower Respiratory Tract of Patients with Different Pulmonary Diseases Based on Metagenomic Next-Generation Sequencing

Provisionally accepted
Rujun Hong Rujun Hong 1Sheng Lin Sheng Lin 1Siting Zhang Siting Zhang 1Yaxing Yi Yaxing Yi 2Lanfeng Li Lanfeng Li 1Haitao Yang Haitao Yang 1Zhenshan Du Zhenshan Du 2Xuefang Cao Xuefang Cao 2Wenjie Wu Wenjie Wu 2Ruotong Ren Ruotong Ren 2,3Xiujuan Yao Xiujuan Yao 1Baosong Xie Baosong Xie 4*
  • 1 Fujian Provincial Hospital, Fuzhou, Fujian Province, China
  • 2 Matridx Biotechnology Co., Ltd, Hangzhou, Zhejiang, China
  • 3 Institute of Biophysics, Chinese Academy of Sciences (CAS), Beijing, Beijing Municipality, China
  • 4 Department of Critical Care Medicine, Fujian Provincial Hospital, Fuzhou, China

The final, formatted version of the article will be published soon.

    The homeostasis of the microbiome in lower respiratory tract is crucial in sustaining normal physiological functions of the lung. Different pulmonary diseases display varying degrees of microbiome imbalance; however, the specific variability and clinical significance of their microbiomes remain largely unexplored. In this study, we delineated the pathogen spectrum and commensal microorganisms in the lower respiratory tract of various pulmonary diseases using metagenomic sequencing. We analyzed the disparities and commonalities of the microbial features and examined their correlation with disease characteristics. We observed distinct pathogen profiles and a diversity in lower airway microbiome in patients diagnosed with cancer, interstitial lung disease, bronchiectasis, common pneumonia, Nontuberculous mycobacteria (NTM) pneumonia, and severe pneumonia. This study illustrates the utility of Metagenomic Next-generation Sequencing (mNGS) in identifying pathogens and analyzing the lower respiratory microbiome, which is important for understanding the microbiological aspect of pulmonary diseases and essential for their early and precise diagnosis.

    Keywords: pulmonary disease, Lower respiratory tract, MNGs, pathogen, microbiome

    Received: 24 Oct 2023; Accepted: 08 Oct 2024.

    Copyright: © 2024 Hong, Lin, Zhang, Yi, Li, Yang, Du, Cao, Wu, Ren, Yao and Xie. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Baosong Xie, Department of Critical Care Medicine, Fujian Provincial Hospital, Fuzhou, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.