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ORIGINAL RESEARCH article

Front. Cell. Infect. Microbiol.
Sec. Molecular Bacterial Pathogenesis
Volume 13 - 2023 | doi: 10.3389/fcimb.2023.1259764
This article is part of the Research Topic Epidemiology of Antimicrobial Resistance and Virulence Factors of Emerging and Re-emerging bacteria View all 11 articles

Pandemic One Health clones of Escherichia coli and Klebsiella pneumoniae producing CTX-M-14, CTX-M-27, CTX-M-55 and CTX-M-65 ESβLs among companion animals in northern Ecuador

Provisionally accepted
Fernando Alexis Gonzales-Zubiate Fernando Alexis Gonzales-Zubiate 1*José Humberto Machado Tambor José Humberto Machado Tambor 2,3Juan Valencia-Bacca Juan Valencia-Bacca 4María Fernanda Villota-Burbano María Fernanda Villota-Burbano 5Adriana Rocio Cardenas-Arias Adriana Rocio Cardenas-Arias 6,7Fernanda Esposito Fernanda Esposito 7,8Quézia Moura Quézia Moura 9Bruna Fuga Bruna Fuga 6,7,8Elder Sano Elder Sano 6Mishell Poleth Ortiz Jacome Mishell Poleth Ortiz Jacome 1Nilton Lincopan Nilton Lincopan 6,7,8*
  • 1 School of Biological Sciences and Engineering, Yachay Tech University, Urcuquí, Ecuador
  • 2 Centro Universitario ENIAC, Guarulhos, Brazil
  • 3 INTI International University, Persiaran Perdana BBN, Putra Nilai, Nilai, Malaysia
  • 4 Department of Microbiology, Wake Forest School of Medicine, Winston Salem, United States
  • 5 Dogs & cats Clinica Veterinaria, Ibarra, Ecuador
  • 6 Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, São Paulo, Brazil
  • 7 One Health Brazilian Resistance Project (OneBR), São Paulo, Rio Grande do Sul, Brazil
  • 8 Department of Clinical Analysis, School of Pharmacy, University of São Paulo, São Paulo, Brazil, São Paulo, Rio Grande do Sul, Brazil
  • 9 Federal Institute of Espírito Santo, Vila Velha, Brazil, Vila Velha, Brazil

The final, formatted version of the article will be published soon.

    From a One Health perspective, dogs and cats have begun to be recognized as important reservoirs for clinically significant multidrug-resistant bacterial pathogens. In this study, we investigated the occurrence and genomic features of ESβL producing Enterobacterales isolated from dogs, in the province of Imbabura, Ecuador. We identified four isolates expressing ESβLs from healthy and diseased animals. In this regard, two Escherichia coli strains producing CTX-M-55-like or CTX-M-65 ESβLs belonged to the international ST10 and ST162, whereas two Klebsiella pneumoniae producing CTX-M-14 or CTX-M-27 belonged to ST35 and ST661. Phylogenomic analysis clustered (95-105 SNP differences) CTX-M-55/ST10 E. coli from companion animal with food and human E. coli strains of ST10 isolated in 2016, in Australia and Cambodia, respectively; whereas CTX-M-27-positive K. pneumoniae ST661 was clustered (201-216 SNP differences) with human strains identified in Italy, in 2013 and 2017, respectively. In summary, we report the presence and genomic data of global human-associated clones of CTX-M-producing E. coli and K. pneumoniae in dogs, in Ecuador. The implementation of a national epidemiological surveillance program is necessary to establish future strategies to control the dissemination of antibiotic-resistant priority pathogens using a One Health approach.

    Keywords: ESβL, Gram-Negative Bacteria, Enterobacterales , antimicrobial resistance, One Health, Veterinary Medicine, genomic data

    Received: 16 Jul 2023; Accepted: 10 Oct 2023.

    Copyright: © 2023 Gonzales-Zubiate, Tambor, Valencia-Bacca, Villota-Burbano, Cardenas-Arias, Esposito, Moura, Fuga, Sano, Jacome and Lincopan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Fernando Alexis Gonzales-Zubiate, School of Biological Sciences and Engineering, Yachay Tech University, Urcuquí, Ecuador
    Nilton Lincopan, One Health Brazilian Resistance Project (OneBR), São Paulo, Rio Grande do Sul, Brazil

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