AUTHOR=Gonzales-Zubiate Fernando A. , Tambor José Humberto M. , Valencia-Bacca Juan , Villota-Burbano María Fernanda , Cardenas-Arias Adriana , Esposito Fernanda , Moura Quézia , Fuga Bruna , Sano Elder , Pariona Jesus G. M. , Jacome Mishell Poleth Ortiz , Lincopan Nilton 

TITLE=Pandemic one health clones of Escherichia coli and Klebsiella pneumoniae producing CTX-M-14, CTX-M-27, CTX-M-55 and CTX-M-65 ESβLs among companion animals in northern Ecuador

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=13

YEAR=2025

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2023.1259764

DOI=10.3389/fcimb.2023.1259764

ISSN=2235-2988

ABSTRACT=<p>From a One Health perspective, dogs and cats have begun to be recognized as important reservoirs for clinically significant multidrug-resistant bacterial pathogens. In this study, we investigated the occurrence and genomic features of ESβL producing Enterobacterales isolated from dogs, in the province of Imbabura, Ecuador. We identified four isolates expressing ESβLs from healthy and diseased animals. In this regard, two <italic>Escherichia coli</italic> strains producing CTX-M-55-like or CTX-M-65 ESβLs belonged to the international ST10 and ST162, whereas two <italic>Klebsiella pneumoniae</italic> producing CTX-M-14 or CTX-M-27 belonged to ST35 and ST661. Phylogenomic analysis clustered (95-105 SNP differences) CTX-M-55/ST10 <italic>E. coli</italic> from companion animal with food and human <italic>E. coli</italic> strains of ST10 isolated in 2016, in Australia and Cambodia, respectively; whereas CTX-M-27-positive <italic>K. pneumoniae</italic> ST661 was clustered (201-216 SNP differences) with human strains identified in Italy, in 2013 and 2017, respectively. In summary, we report the presence and genomic data of global human-associated clones of CTX-M-producing <italic>E. coli</italic> and <italic>K. pneumoniae</italic> in dogs, in Ecuador. The implementation of a national epidemiological surveillance program is necessary to establish future strategies to control the dissemination of antibiotic-resistant priority pathogens using a One Health approach.</p>