Frontiers in Bioengineering and Biotechnology

Original Research

Published on 10 Nov 2023

Pyramiding stacking of multigenes (PSM): a simple, flexible and efficient multigene stacking system based on Gibson assembly and gateway cloning

in Synthetic Biology

Dongdong Zeng
Cuiyuan Jing
Lin Tang
Peng He
Jie Zhang

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2023.1263715

2,834 views
2 citations

Original Research

Published on 06 Nov 2023

Enhancing isoprenol production by systematically tuning metabolic pathways using CRISPR interference in E. coli

in Synthetic Biology

Jinho Kim
Taek Soon Lee

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2023.1296132

2,808 views
3 citations

Correction

Published on 06 Nov 2023

Corrigendum: The main protease 3CLpro of the SARS-CoV-2 virus: how to turn an enemy into a helper

in Synthetic Biology

Svetlana V. Belenkaya
Iuliia A. Merkuleva
Olga I. Yarovaya
Varvara Yu Chirkova
Elena A. Sharlaeva
Daniil V. Shanshin
Ekaterina A. Volosnikova
Sergey Z. Vatsadze
Mikhail V. Khvostov
Nariman F. Salakhutdinov

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2023.1294266

956 views
1 citation

Original Research

Published on 30 Oct 2023

Expanding structural diversity of 5′-aminouridine moiety of sansanmycin via mutational biosynthesis

in Synthetic Biology

Yuan Lu
Yihong Li
Jiahui Fan
Xingxing Li
Hongmin Sun
Lifei Wang
Xingli Han
Yuting Zhu
Tianyu Zhang
Yuanyuan Shi

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2023.1278601

1,691 views
1 citation

Review

Published on 26 Oct 2023

CRISPR-based bioengineering in microalgae for production of industrially important biomolecules

in Synthetic Biology

Dhananjay Dhokane
Arshi Shaikh
Anu Yadav
Nandinee Giri
Anindya Bandyopadhyay
Santanu Dasgupta
Bhaskar Bhadra

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2023.1267826

9,453 views
14 citations

Review

Published on 19 Oct 2023

Systems and synthetic biology-driven engineering of live bacterial therapeutics

in Synthetic Biology

Kangsan Kim
Minjeong Kang
Byung-Kwan Cho

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2023.1267378

5,287 views
7 citations

Original Research

Published on 18 Oct 2023

Quantitative and modularized CRISPR/dCas9-dCpf1 dual function system in Saccharomyces cerevisiae

in Synthetic Biology

Qing Feng
Xiaoyu Ning
Lei Qin
Jun Li
Chun Li

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2023.1218832

2,716 views

Original Research

Published on 17 Oct 2023

Targeted control of supporting pathways in paclitaxel biosynthesis with CRISPR-guided methylation

in Synthetic Biology

Cassandra Brzycki Newton
Eric M. Young
Susan C. Roberts

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2023.1272811

3,034 views
7 citations

Original Research

Published on 12 Oct 2023

Transcriptomics of Lactobacillus paracasei: metabolism patterns and cellular responses under high-density culture conditions

in Synthetic Biology

Liangzhi Li
Hetian Zhang
Delong Meng
Huaqun Yin

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2023.1274020

3,277 views
1 citation

Review

Published on 10 Oct 2023

Advancements in the synergy of isothermal amplification and CRISPR-cas technologies for pathogen detection

in Synthetic Biology

Xiaolei Mao
Minghui Xu
Shuyin Luo
Yi Yang
Jiaye Zhong
Jiawei Zhou
Huayan Fan
Xiaoping Li
Zhi Chen

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2023.1273988

5,275 views
9 citations

Review

Published on 05 Oct 2023

An outlook to sophisticated technologies and novel developments for metabolic regulation in the Saccharomyces cerevisiae expression system

in Synthetic Biology

Yijian Wu
Sai Feng
Zeao Sun
Yan Hu
Xiao Jia
Bin Zeng

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2023.1249841

4,203 views
4 citations

Original Research

Published on 22 Sep 2023

Optimizing CRE and PhiC31 mediated recombination in Aedes aegypti

in Synthetic Biology

Leonela Z. Carabajal Paladino
Ray Wilson
Priscilla Y. L. Tng
Vishaal Dhokiya
Elizabeth Keen
Piotr Cuber
Will Larner
Sara Rooney
Melanie Nicholls
Anastasia Uglow

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2023.1254863

2,455 views
1 citation

Brief Research Report

Published on 12 Sep 2023

Hybrid Boolean gates show that Cas12c controls transcription activation effectively in the yeast S. cerevisiae

in Synthetic Biology

Yifan Liu
Huanhuan Ge
Mario Andrea Marchisio

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2023.1267174

1,476 views
1 citation

Original Research

Published on 12 Sep 2023

VNAR development through antigen immunization of Japanese topeshark (Hemitriakis japanica)

in Synthetic Biology

Hiroyuki Takeda
Tatsuhiko Ozawa
Hiroki Zenke
Yoh Ohnuki
Yuri Umeda
Wei Zhou
Honoka Tomoda
Akihiko Takechi
Kimiyoshi Narita
Takaaki Shimizu

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2023.1265582

4,869 views
5 citations

Mini Review

Published on 31 Aug 2023

Advances in the application of recombinase-aided amplification combined with CRISPR-Cas technology in quick detection of pathogenic microbes

in Synthetic Biology

Xiaoping Li
Shuying Zhu
Xinling Zhang
Yanli Ren
Jing He
Jiawei Zhou
Liliang Yin
Gang Wang
Tian Zhong
Ling Wang

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2023.1215466

6,480 views
22 citations

Methods

Published on 29 Aug 2023

gRNA-SeqRET: a universal tool for targeted and genome-scale gRNA design and sequence extraction for prokaryotes and eukaryotes

in Synthetic Biology

Lisa Simirenko
Jan-Fang Cheng
Ian Blaby

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2023.1217811

2,296 views
2 citations