Frontiers in Bioengineering and Biotechnology

Opinion

Accepted on 07 Mar 2025

CGT 4.0: a distant dream or inevitable future? Smart process automation is critical to make efficient scalability of CGT manufacturing a reality

in Cell and Gene Therapy

Aleksander Szarzynski
Oliver Spadiut
Mathias Reisbeck
Gerhard Jobst
Rachel L. Paterson
Anna Kamenskaya
Emilie Gateau
Hanna P. Lesch
Luc Henry
Bence Kozma

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1563878

364 views

Original Research

Accepted on 05 Mar 2025

Towards propagation of epidermal cells for wound repair: Glass, as cell culture substrate, enhances proliferation and migration of human keratinocytes

in Cell and Gene Therapy

Hady Shahin
Ingrid Steinvall
Folke Sjoberg
Moustafa Elmasry
Ahmed El-Serafi

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1547044

Original Research

Accepted on 03 Mar 2025

Lymphoblastoid and Jurkat cell lines are useful surrogate in developing a CRISPR-Cas9 method to correct Leukocyte adhesion deficiency genomic defect.

in Cell and Gene Therapy

Ahmad Ramadan
Noureddine Ben Khalaf
Khaled Trabelsi
HeLa Bakhiet
Imen Ben Mustapha
Mohamed Ridha Barbouche
M-Dahmani Fathallah

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1548227

Original Research

Published on 05 Feb 2025

The landscape of cell regulatory and communication networks in the human dental follicle

in Cell and Gene Therapy

Jia-Ning Liu
Jiong-Yi Tian
Lu Liu
Yuan Cao
Xiao Lei
Xiao-Hui Zhang
Zi-Qi Zhang
Jun-Xi He
Chen-Xi Zheng
Chao Ma

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1535245

642 views

Review

Published on 29 Jan 2025

Application of biomaterials in mesenchymal stem cell based endometrial reconstruction: current status and challenges

in Cell and Gene Therapy

Ling He
Qianrong Li

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1518398

905 views

Original Research

Published on 23 Jan 2025

A systematic screening assay identifies efficient small guide RNAs for CRISPR activation

in Cell and Gene Therapy

Elin Arvidsson
Diana Duarte Lobo
Ermelinda Sabarese
Fabio Duarte
Rui Jorge Nobre
Luis Quintino
Cecilia Lundberg

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1336313

866 views

Review

Published on 15 Jan 2025

Approaches and applications in transdermal and transpulmonary gene drug delivery

in Cell and Gene Therapy

Anni Zhang
Xuran Zhang
Jiahui Chen
Xianbao Shi
Xijuan Yu
Zhonggui He
Jin Sun
Mengchi Sun
Zhijun Liu

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2024.1519557

923 views

Methods

Published on 20 Nov 2024

Device for automated aseptic sampling: Automated sampling solution for future cell and gene manufacturing

in Cell and Gene Therapy

Liu Dan
Wu Ying Ying
Akshaya V. Prabhu
Ahmad Amirul bin Abdul Rahim
Zach Lee Jia Sheng

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2024.1452674

1,259 views

Original Research

Published on 20 Nov 2024

Protein trans-splicing: optimization of intein-mediated GFP assembly as a model for the development of gene therapy

in Cell and Gene Therapy

Andrew Brovin
Ekaterina Minskaia
Matvei Sabantsev
Sergey Chuvpilo
Alexander Karabelsky

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2024.1488912

2,145 views

Original Research

Published on 11 Nov 2024

Morphogen-driven differentiation is precluded by physical confinement in human iPSCs spheroids

in Cell and Gene Therapy

Haneen S. Alsehli
Errin Roy
Thomas Williams
Alicja Kuziola
Yunzhe Guo
Cecile A. Dreiss
Jeremy B.A. Green
Eileen Gentleman
Davide Danovi

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2024.1467412

1,611 views

Original Research

Published on 01 Oct 2024

An improved high-resolution method for quantitative separation of empty and filled AAV8 capsids by strong anion exchange HPLC

in Cell and Gene Therapy

Samantha Schrecke
Kevin McManus
Cassandra Moshfegh
Jessica Stone
Thuy-Uyen Nguyen
Gustavo Rivas
Ismaeel Muhamed
Daniel A. J. Mitchell

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2024.1436857

2,298 views
1 citation

Original Research

Published on 20 Aug 2024

Assessing a single-cell multi-omic analytic platform to characterize ex vivo-engineered T-cell therapy products

in Cell and Gene Therapy

Maryam Moshref
Jerry Hung-Hao Lo
Andrew McKay
Julien Camperi
Joseph Schroer
Norikiyo Ueno
Shu Wang
Saurabh Gulati
Somayeh Tarighat
Steffen Durinck

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2024.1417070

7,077 views

Review

Published on 29 Jul 2024

The application potential of iMSCs and iMSC-EVs in diseases

in Cell and Gene Therapy

Xin Zhou
Jinyu Liu
Feifeng Wu
Jueyi Mao
Yang Wang
Junquan Zhu
Kimsor Hong
Haotian Xie
Binbin Li
Xinying Qiu

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2024.1434465

2,544 views
1 citation

Systematic Review

Published on 22 Jul 2024

Effectiveness and mechanisms of mesenchymal stem cell therapy in preclinical animal models of hepatic fibrosis: a systematic review and meta-analysis

in Cell and Gene Therapy

Xuesong Wang
Yue Wang
Wenming Lu
Jiayang Qu
Yang Zhang
Junsong Ye

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2024.1424253

1,944 views
1 citation

Original Research

Published on 10 Jul 2024

Effects of buffer composition and plasmid toxicity on electroporation-based non-viral gene delivery in mammalian cells using bursts of nanosecond and microsecond pulses

in Cell and Gene Therapy

Eivina Radzevičiūtė-Valčiukė
Jovita Gečaitė
Austėja Balevičiūtė
Anna Szewczyk
Augustinas Želvys
Barbora Lekešytė
Veronika Malyško-Ptašinskė
Eglė Mickevičiūtė
Paulina Malakauskaitė
Julita Kulbacka

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2024.1430637

1,968 views

Original Research

Published on 05 Jul 2024

11β hydroxysteroid dehydrogenase type 1 transgenic mesenchymal stem cells attenuate inflammation in models of sepsis

in Cell and Gene Therapy

Rahul Y. Mahida
Zhengqiang Yuan
Krishna K. Kolluri
Aaron Scott
Dhruv Parekh
Rowan S. Hardy
Michael A. Matthay
Gavin D. Perkins
Sam M. Janes
David R. Thickett

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2024.1422761

2,880 views

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Articles

CGT 4.0: a distant dream or inevitable future? Smart process automation is critical to make efficient scalability of CGT manufacturing a reality

Towards propagation of epidermal cells for wound repair: Glass, as cell culture substrate, enhances proliferation and migration of human keratinocytes

Lymphoblastoid and Jurkat cell lines are useful surrogate in developing a CRISPR-Cas9 method to correct Leukocyte adhesion deficiency genomic defect.

The landscape of cell regulatory and communication networks in the human dental follicle

Application of biomaterials in mesenchymal stem cell based endometrial reconstruction: current status and challenges

A systematic screening assay identifies efficient small guide RNAs for CRISPR activation

Approaches and applications in transdermal and transpulmonary gene drug delivery

Device for automated aseptic sampling: Automated sampling solution for future cell and gene manufacturing

Protein trans-splicing: optimization of intein-mediated GFP assembly as a model for the development of gene therapy

Morphogen-driven differentiation is precluded by physical confinement in human iPSCs spheroids

An improved high-resolution method for quantitative separation of empty and filled AAV8 capsids by strong anion exchange HPLC

Assessing a single-cell multi-omic analytic platform to characterize ex vivo-engineered T-cell therapy products

The application potential of iMSCs and iMSC-EVs in diseases

Effectiveness and mechanisms of mesenchymal stem cell therapy in preclinical animal models of hepatic fibrosis: a systematic review and meta-analysis

Effects of buffer composition and plasmid toxicity on electroporation-based non-viral gene delivery in mammalian cells using bursts of nanosecond and microsecond pulses

11β hydroxysteroid dehydrogenase type 1 transgenic mesenchymal stem cells attenuate inflammation in models of sepsis