Frontiers in Bioengineering and Biotechnology

Original Research

Accepted on 17 Sep 2025

Production of high-affinity glycosylated anti-mouse conjugated nanobodies in Pichia pastoris

in Bioprocess Engineering

Sofía Orioli
Javier Santos
Lorena Itatí Ibañez
Cecilia D'Alessio

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1673481

Original Research

Published on 16 Sep 2025

Viable cell sorting by fluidized bed centrifugation enables novel cultivation strategies

in Bioprocess Engineering

Martin Saballus
Lucas Nik Reger
Robin Obser
Julia Niemann
Rene H. Wijffels
Dirk E. Martens
Markus Kampmann

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1667343

1,163 views

Original Research

Published on 15 Sep 2025

Metabolome profile and microbial community structure of Cenchrus fungigraminus silage under different moisture gradients

in Bioprocess Engineering

Xiaohan Hou
Xiaohui Chu
Yang Yang
Xue Xiao
Qiongmei Niu
Guilian Shan

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1657657

612 views

Original Research

Published on 12 Sep 2025

Removal of hexavalent chromium by a microbial mat from a mining site under anaerobic conditions

in Bioprocess Engineering

Mohammad Tariq Ali Khan
Sumaiya Said Al-Siyabi
Hamada E. Ali
Raeid M. M. Abed

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1585237

904 views

Retraction

Accepted on 09 Sep 2025

Retraction: Purification, Characterization, and Hydrolysate Analysis of Dextranase From Arthrobacter oxydans G6-4B

in Bioprocess Engineering

Frontiers Editorial Office

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1702209

338 views

Brief Research Report

Published on 09 Sep 2025

Selective and mild oxidation of α-hydroxyl groups in lignin by 2-iodoxybenzoic acid

in Bioprocess Engineering

Fei Jiang
Zhiming Dong
Xiaoli Lou
Zhuonan Zhang
Li Shuai
Chengke Zhao

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1654739

898 views

Perspective

Published on 03 Sep 2025

Isolator-based point-of-care manufacturing: a practical solution for GMP-compliant cell and extracellular vesicles therapy production

in Bioprocess Engineering

Yu-Sung Chiu
Kun-Liang Wang
Kun-Lieh Wu

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1644318

1,499 views

Original Research

Accepted on 29 Aug 2025

Advancing the predictive techno-economic and lifecycle assessment of prairie grass and manure co-digestion for renewable natural gas applications

in Bioprocess Engineering

Katherine Wild
Juan Carlos Domínguez
Lisa Schulte Moore
Mark Mba Wright

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1651510

745 views

Original Research

Accepted on 29 Aug 2025

Development and validation of a qPCR assay for detection of residual host cell DNA for rabies vaccines produced in Vero cells

in Bioprocess Engineering

Danhua Zhao
Weiying Zong
Wanxin Wu
Li Yuhua
Zongsong Wu
Zhixing Yang
Shouchun Cao

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1611428

435 views

Mini Review

Published on 21 Aug 2025

Identification of critical process parameters and quality attributes for bioreactor-based expansion of human MSCs

in Bioprocess Engineering

Laura Herbst
Bastian Nießing
Robert H. Schmitt

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1608194

1,572 views

Original Research

Published on 21 Aug 2025

Raman-based PAT for multi-attribute monitoring during VLP recovery by dual-stage CFF: attribute-specific spectral preprocessing for model transfer

in Bioprocess Engineering

Annabelle Dietrich
Luca Heim
Jürgen Hubbuch

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1631807

1,255 views

Original Research

Accepted on 20 Aug 2025

Prospects and challenges of recombinant spider venom enzymes: insights from Loxosceles and Phoneutria venom protease expressions

in Bioprocess Engineering

Josephine Dresler
Ivonne Rodriguez
Anne Paas
Alfredo Cabrera-Orefice
Andreas Vilcinskas
Tim Lüddecke

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1668774

351 views

Original Research

Published on 06 Aug 2025

Development and optimization of an ammonia removal strategy for sustainable recycling of cell culture spent media in cultivated meat production: from concept to implementation

in Bioprocess Engineering

Babak Pakbin
Armaghan Amanipour
Arian Amirvaresi
Arash Shahsavari
Reza Ovissipour

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1617115

1,621 views

Original Research

Published on 01 Aug 2025

Evaluation of a single-use bioartificial liver (BAL) biocartridge consisting of cryopreservable alginate encapsulated liver cell spheroids as a component of HepatiCan™, a novel bioartificial liver device

in Bioprocess Engineering

Eloy Erro
Tom Brookshaw
Barry Fuller
Sweta Chandel
Joana Mendonca da Silva
Elizaveta Zotova
Sherri-Ann Chalmers
Alfie Watt
Clare Selden

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1572254

2,386 views

Original Research

Published on 01 Aug 2025

Enhanced cancer cell sorting using lab-on-a-disk pattern design with magnetic and centrifugal forces

in Bioprocess Engineering

Bill Cheng
Wei-Cheng Chao
Yi-Han Chen
Yao-Tsung Lin

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1611313

1,194 views

Review

Published on 31 Jul 2025

Advances in alginate biosynthesis: regulation and production in Azotobacter vinelandii

in Bioprocess Engineering

Belén Ponce
Agustín Zamora-Quiroz
Ernesto González
Rodrigo Andler
Alvaro Díaz-Barrera

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1593893

1,640 views