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ORIGINAL RESEARCH article

Front. Bioeng. Biotechnol.
Sec. Biomechanics
Volume 12 - 2024 | doi: 10.3389/fbioe.2024.1514483
This article is part of the Research Topic Biomechanical and Biomaterial Advances in Degenerative Diseases of Bone and Joint View all 10 articles

Polydatin retards the progression of osteoarthritis by maintaining bone metabolicbalance and inhibiting macrophage polarization

Provisionally accepted
Qi Sun Qi Sun 1Yu Xin Nan Yu Xin Nan 2*Hui Wang Hui Wang 1*Shuo Pan Shuo Pan 1*Gang Ji Gang Ji 1*Feng Ya Guo Feng Ya Guo 1*Heng Ya Zhao Heng Ya Zhao 1*Cen Gao Li Cen Gao Li 1*Shi Shao Guo Shi Shao Guo 1*Feng Lu Lin Feng Lu Lin 1*Jie Yu Jin Jie Yu Jin 1*Li Xue Zhang Li Xue Zhang 1*Cheng Chang Liu Cheng Chang Liu 1*Bin Guo Liu Bin Guo Liu 1*
  • 1 The First hospital of Hebei Medical University, Shijiazhuang, China
  • 2 Hebei Medical University, Shijiazhuang, Hebei Province, China

The final, formatted version of the article will be published soon.

    Background:Polydatin (PD), also known as tiger cane glycoside, is a natural compound extracted from the Japanese knotweed plant, which is often referred to as white resveratrol. It exhibits anti-inflammatory, antioxidant, and anti-apoptotic effects in the treatment of various diseases. However, the potential molecular mechanisms of PD in osteoarthritis have not been clearly elucidated.Methods: Anterior cruciate ligament transection (ACLT) surgery was performed to establish an osteoarthritis animal model. Female mice at the age of 12 weeks were intraperitoneally injected with different concentrations of PD (20 and 40mg/kg). In vitro models were established by isolating mouse articular chondrocytes, which were subsequently treated with lipopolysaccharide or IL-1β for 24 hours for subsequent experiments. In addition, different concentrations of PD were administered for 12 hours. Morphological changes were observed by toluidine blue staining, joint bone metabolism changes were observed by tartrate-resistant acid phosphatase staining, immunohistochemistry was used to observe the expression levels of inflammatory factors and extracellular matrix. MicroCT analysis was conducted to assess changes in the microstructure of subchondral bone trabeculae, and Western blot was performed to measure the expression of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling pathway and markers of M1 polarization in macrophages.Results: PD significantly delays the progression of osteoarthritis induced by ACLT, effectively inhibits IL-1β-induced joint inflammation, bone metabolic remodeling and extracellular matrix degradation. In addition, paeoniflorin markedly suppresses the transmission of the NF-κB signaling pathway and reverses M1 polarization in macrophages induced by IL-1β.Conclusions: Taken together, PD might be a potential therapeutic agent for the prevention and treatment of osteoarthritis.

    Keywords: Polydatin, Cartilage, Extracellular Matrix, NF-κB, Osteoarthritis

    Received: 21 Oct 2024; Accepted: 17 Dec 2024.

    Copyright: © 2024 Sun, Nan, Wang, Pan, Ji, Guo, Zhao, Li, Guo, Lin, Jin, Zhang, Liu and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Yu Xin Nan, Hebei Medical University, Shijiazhuang, 050017, Hebei Province, China
    Hui Wang, The First hospital of Hebei Medical University, Shijiazhuang, China
    Shuo Pan, The First hospital of Hebei Medical University, Shijiazhuang, China
    Gang Ji, The First hospital of Hebei Medical University, Shijiazhuang, China
    Feng Ya Guo, The First hospital of Hebei Medical University, Shijiazhuang, China
    Heng Ya Zhao, The First hospital of Hebei Medical University, Shijiazhuang, China
    Cen Gao Li, The First hospital of Hebei Medical University, Shijiazhuang, China
    Shi Shao Guo, The First hospital of Hebei Medical University, Shijiazhuang, China
    Feng Lu Lin, The First hospital of Hebei Medical University, Shijiazhuang, China
    Jie Yu Jin, The First hospital of Hebei Medical University, Shijiazhuang, China
    Li Xue Zhang, The First hospital of Hebei Medical University, Shijiazhuang, China
    Cheng Chang Liu, The First hospital of Hebei Medical University, Shijiazhuang, China
    Bin Guo Liu, The First hospital of Hebei Medical University, Shijiazhuang, China

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