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ORIGINAL RESEARCH article
Front. Anim. Sci.
Sec. Animal Breeding and Genetics
Volume 6 - 2025 | doi: 10.3389/fanim.2025.1513876
Genome-wide scanning for candidate lethal genes associated with early embryonic mortality in Holstein dairy cattle
Provisionally accepted- 1 Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Gilan, Iran
- 2 University of Guilan, Rasht, Iran
- 3 Skolkovo Institute of Science and Technology, Moscow, Moscow Oblast, Russia
Objective: Embryo death in the early stages, primarily caused by lethal alleles in the homozygous state, is one of the important challenges in dairy cattle breeding. Methods: The availability of large-scale genomic SNP data has proven to be a promising tool for identifying recessive genetic defects. This study was conducted to identify lethal alleles and genes causing embryo death in Holstein dairy cattle using omics data in genomic and transcriptomic level. High-density Bovine770K SNP array genotypes of 3117 samples and whole-genome sequences (WGS) of 743 cows were utilized to identify lethal SNPs, defined as those markers significantly departing from Hardy–Weinberg equilibrium and lacking one of the homozygous genotypes. Results: The potential candidate lethal SNPs are harbored by PARD3, BAHD1, FZD3, ERGIC2, IQCN, PROK1, PCTP, SH3GLB1, and RASSF5 genes, according to the ARS-UCD1.2 Bos taurus genome assembly. Transcriptome analysis showed that these genes are steadily expressed in the various embryonic tissues during different stages of embryo development, and therefore were considered as the potential candidate lethal genes. These genes play important roles in various biological processes of embryo development and prenatal survival ability in dairy cattle embryos. These genes contribute to the important embryo lethality-related mechanisms, including the regulation of cell polarity, placental development, phospholipid transport, and apoptosis. Conclusion: The findings of this study provide insight into the complex molecular mechanisms of embryo mortality at the early stage of pregnancy caused by genes following the recessive inheritance model.
Keywords: Embryonic lethality, Genomic, Holstein dairy cattle, Recessive alleles, transcriptomic
Received: 19 Oct 2024; Accepted: 13 Feb 2025.
Copyright: © 2025 Rezaie, Shadparvar, Baneh and Ghoavti. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Abdol Ahad Shadparvar, University of Guilan, Rasht, Iran
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