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ORIGINAL RESEARCH article

Front. Allergy
Sec. Allergy Diagnosis
Volume 5 - 2024 | doi: 10.3389/falgy.2024.1496882
This article is part of the Research Topic Innovations in Allergy Diagnostics and Management: A Global Perspective View all articles

Comparison of the New RIDA qLine Allergy Multiparameter Immunoblot and the ImmunoCAP Specific IgE Test for the Identification of Clinically Relevant Food-and Aeroallergen Allergies

Provisionally accepted
Katharina K. Hahn Katharina K. Hahn *Marie C. Schuppe Marie C. Schuppe Moritz M. Hollstein Moritz M. Hollstein Susann Forkel Susann Forkel Timo Buhl Timo Buhl
  • Department of Dermatology, Venereology and Allergology, University Medical Center Göttingen, Göttingen, Lower Saxony, Germany

The final, formatted version of the article will be published soon.

    Background: Multiparameter immunoblot testing is increasingly used as an alternative to multiple individual IgE analyses for type 1 allergies. This study investigated the performance of the inexpensive immunoblot method RIDA qLine Allergy Test System (R-Biopharm AG) versus the current gold standard.Methods: Three study-specific panels with 57 individual allergens (food and aeroallergens) were analyzed in serum samples from 200 patients with signs and symptoms of IgE-mediated allergies, using both the RIDA qLine Allergy and the reference method ImmunoCAP Specific IgE Test (Thermo Fisher Scientific). In case of divergent results, corresponding allergens were remeasured using the secondary reference method 3gAllergy Specific IgE Universal Kit (Siemens). Clinical diagnoses of the 200 patients were included. Additionally, a cross-reactive carbohydrate determinants (CCD)-inhibitor was tested to decrease the incidence of positive CCD-bands.Results: The mean overall agreement of all food and aeroallergens with the reference methods was 94.9%. Qualitative evaluation showed a negative percentage agreement of 98.9% and a positive percentage agreement of 75.1% averaged over all individual allergens after testing with both reference methods. Additional treatment of samples with the CCD-inhibitor successfully reduced the occurrence of positive CCD reactivity after retesting.The comparative analysis of RIDA qLine Allergy with the reference methods for specific IgE detection revealed a strong correlation between serum IgE levels measured across these platforms and clinical presentations, while also highlighting the necessity for careful contextual interpretation of results. Standardized allergen extracts would improve independent comparison of different allergy testing methods.

    Keywords: Aeroallergen, Food allergen, IgE testing, immunoblot, Multiparameter

    Received: 15 Sep 2024; Accepted: 17 Dec 2024.

    Copyright: © 2024 Hahn, Schuppe, Hollstein, Forkel and Buhl. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Katharina K. Hahn, Department of Dermatology, Venereology and Allergology, University Medical Center Göttingen, Göttingen, 37075, Lower Saxony, Germany

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.