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ORIGINAL RESEARCH article

Front. Allergy
Sec. Asthma
Volume 5 - 2024 | doi: 10.3389/falgy.2024.1462579

Low CD46 expression on activated CD4 + T cells predict improved Th1 cell reactivity to calcitriol in majority of patients with allergic eosinophilic asthma and healthy donors

Provisionally accepted
Julie Stichova Julie Stichova 1,2Peter Slanina Peter Slanina 1Zita Chovancova Zita Chovancova 1,2Jan Baros Jan Baros 1,2Marek Litzman Marek Litzman 3Jiri Litzman Jiri Litzman 1,2Marcela Vlkova Marcela Vlkova 1,2*
  • 1 Faculty of Medicine, Masaryk University, Brno, South Moravia, Czechia
  • 2 St. Anne's University Hospital Brno, Brno, South Moravia, Czechia
  • 3 Department of Economics, Faculty of Business and Economics, Mendel University in Brno, Czech Republic, Brno, Czechia

The final, formatted version of the article will be published soon.

    Background: Previous research showed that the intracellular complement system, with CD46 as its central molecule, regulates the Th1 response associated with IFN-γ production and transition to a type 1 regulatory response (Tr1) characterized by IL-10 production. This transition can be influenced by a vitamin D (calcitriol), favouring a shift towards Tr1 cells and increased IL-10 production, as described in some autoimmune diseases.Objective: It is unknown whether calcitriol modulates CD46-induced Th1 response towards regulatory type 1 T cells (Tr1) in allergic eosinophilic asthma and its value in relation to reducing inflammatory response.Methods: CD4 + T cells from 58 patients with allergic eosinophilic asthma (AEA) and 49 healthy donors (HDs) were stimulated with αCD3/αCD46/IL-2 or αCD3/αCD46/IL-2/Calcitriol in vitro for 60h and analyzed by flow cytometry. IFN-γ and IL-10 levels in cell culture supernatants were measured using ELISA.Results: CD4 + T cells from patients with AEA demonstrated elevated CD46 expression in both the non-activated state and under stimulation conditions with αCD3/αCD46/IL-2 or αCD3/αCD46/IL-2/calcitriol. Moreover, CD46 expression in AEA patients fluctuated with the pollen season, showing a significant increase during period of low pollen exposure. Calcitriol

    Keywords: allergic eosinophilic asthma, Calcitriol, CD46, IFN-γ, IL-10

    Received: 10 Jul 2024; Accepted: 10 Sep 2024.

    Copyright: © 2024 Stichova, Slanina, Chovancova, Baros, Litzman, Litzman and Vlkova. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Marcela Vlkova, Faculty of Medicine, Masaryk University, Brno, 625 00, South Moravia, Czechia

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.