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ORIGINAL RESEARCH article

Front. Allergy
Sec. Therapies and Therapeutic Targets
Volume 5 - 2024 | doi: 10.3389/falgy.2024.1437600
This article is part of the Research Topic Fibroblasts & allergy- Advanced molecular targets and therapeutic insights View all articles

Correlation between Forkhead Box P3 (rs3761548) Gene Polymorphism and Serum Interleukin13 as Biomarkers of Severity in Egyptian Allergic Conjunctivitis: A Retrospective Study

Provisionally accepted
Wesam A. Hafez Boghdady Wesam A. Hafez Boghdady 1Marwa A. Khairy Marwa A. Khairy 2Ali G. Ali Ali G. Ali 2Alia A. El Shahaway Alia A. El Shahaway 1*Eman A. Abdelaziz Eman A. Abdelaziz 3Aya A. El Shahawy Aya A. El Shahawy 4Fatma Kamel Fatma Kamel 1
  • 1 Department of Medical Microbiology and Immunology, Faculty of Medicine, Zagazig University, Zagazig, Egypt
  • 2 Faculty of Medicine, Zagazig University, Zagazig, Al Sharqia, Egypt
  • 3 Medical Biochemistry and Molecular Biology, Faculty of Medicine, Zagazig University, Zagazig, Egypt
  • 4 Clinical Pathology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt

The final, formatted version of the article will be published soon.

    The genetic variants that alter human Forkhead Box P3 (FOXP3) function may have a part in the establishment of allergic conjunctivitis. Our study aimed to evaluate the FOXP3 polymorphism, serum interleukin13 (IL13) and total immunoglobulin E (IgE) levels in allergic conjunctivitis and assess their role as biomarkers for allergic conjunctivitis risk and severity. This study included 52 cases and 52 controls. Blood samples were taken from allergic conjunctivitis patients and controls for total IgE, IL13 measurement and detection of FOXP3 (rs3761548) gene polymorphism. There was a statistically significant difference between the allergic conjunctivitis group and healthy control group regarding FOXP3 (rs3761548) polymorphism with those have AA genotype are 12 times at risk for allergic conjunctivitis and A allele increases the risk of allergic conjunctivitis by about 4 times. There was statistically significant difference between mild/ moderate and severe allergic conjunctivitis regarding FOXP3 (rs3761548) polymorphism with those have AA genotype are 53 times at risk for severe allergic conjunctivitis and A allele increases the risk of severe allergic conjunctivitis by about 6 times. Also, there was a significantly higher value of total IgE IU/ml, IL13 Pg/ml value in severe allergic conjunctivitis compared to moderate/ mild allergic conjunctivitis. The best cutoff values of total IgE and serum IL13 for detecting the severity of allergic conjunctivitis were ≥320IUmL and ≥40 Pg/ml and the area under the curve were 0.89 and 0.95 respectively. The research significantly contributes to find correlation of FOXP3 polymorphism, total IgE and IL13 with risk and severity of allergic conjunctivitis which are limited in the literature on the perceived value relevance of FOXP3 polymorphism in allergic conjunctivitis risk and severity.

    Keywords: allergy, allergic conjunctivitis, Forkhead box P3, polymorphism, Interleukin 13, Total immunoglobulin E, regulatory T cells

    Received: 24 May 2024; Accepted: 02 Sep 2024.

    Copyright: © 2024 Hafez Boghdady, A. Khairy, Ali, El Shahaway, Abdelaziz, El Shahawy and Kamel. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Alia A. El Shahaway, Department of Medical Microbiology and Immunology, Faculty of Medicine, Zagazig University, Zagazig, Egypt

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