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ORIGINAL RESEARCH article

Front. Vet. Sci.
Sec. Livestock Genomics
Volume 11 - 2024 | doi: 10.3389/fvets.2024.1520155
This article is part of the Research Topic Vetinformatics: An Insight for Decoding Livestock Systems Through In Silico Biology Volume II View all 6 articles

Genome-Wide Association Studies with Prolapsed Gland of The Third Eelid in Dogs

Provisionally accepted
Qianyong Yang Qianyong Yang 1*Yu Zeng Yu Zeng 1Cundong Feng Cundong Feng 2Zheli Jiang Zheli Jiang 1Jianyun Liu Jianyun Liu 1Xingnuan Li Xingnuan Li 1Shan He Shan He 1Yi Fan Yi Fan 1Xiao Ouyang Xiao Ouyang 1Bixin Huang Bixin Huang 1Yan Su Yan Su 1Siyu Wang Siyu Wang 1Rongxing Wei Rongxing Wei 3Zonghao Dai Zonghao Dai 3Peng Jin Peng Jin 3Weian Du Weian Du 4
  • 1 Jiujiang University, Jiujiang, China
  • 2 Hainan University, Haikou, Hainan Province, China
  • 3 Nanchang Police Dog Base of the Ministry of Public Security, Nanchang, Jiangxi Province, China
  • 4 Foshan University, Foshan, Guangdong Province, China

The final, formatted version of the article will be published soon.

    Cherry eye, the common name for the prolapse of the third eyelid gland in dogs, is a widespread ophthalmic disease affecting dogs of various breeds. This condition severely affects the quality of life of affected dogs, and its underlying cause remains unresolved. In this study, 170K SNP microarray data were collected from 653 brachycephalic dogs and 788 brachycephalic and mesocephalic dogs. These two datasets were analyzed separately in genome-wide association studies (GWAS) involving 12 dog breeds affected by cherry eye. The GWAS analysis of 653 short-headed dogs revealed that four SNPs in the CFA3:15627075-15983629 bp region exceeded the genome-level significance threshold. Association analysis of this region also indicated that these four SNPs were strongly associated. Gene annotation showed that the region contained genes such as KIAA0825, FAM172A, and NR2F1, of which NR2F1 was associated with eye development. The results showed that GWAS analysis performed on 788 short-and medium-headed dogs identified five SNPs in the CFA22:15627075-15983629 bp region that exceeded the genome-level significance threshold, and association analysis was performed in this region, which showed that these five SNPs were strongly associated. In addition, 104 annotated genes were identified in both GWAS. To explore the genes involved in cherry eyes, we performed GO functional enrichment analysis. The genes involved in the high pathway were DIO3 and TTC8. In addition, an in-depth analysis revealed 33 genes associated with eye development and diseases. Our study provides new perspectives for further understanding cherry eye in dogs.

    Keywords: Cherry eye, Third eyelid gland, animal model, canine, Eye Diseases, Genetics

    Received: 30 Oct 2024; Accepted: 30 Dec 2024.

    Copyright: © 2024 Yang, Zeng, Feng, Jiang, Liu, Li, He, Fan, Ouyang, Huang, Su, Wang, Wei, Dai, Jin and Du. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Qianyong Yang, Jiujiang University, Jiujiang, China

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