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ORIGINAL RESEARCH article

Front. Nutr.
Sec. Nutrition and Metabolism
Volume 11 - 2024 | doi: 10.3389/fnut.2024.1435051
This article is part of the Research Topic Resistant Starch: Advances and Applications in Nutrition for Disease Prevention View all 10 articles

Effect of dietary amylose/amylopectin ratio on antioxidant ability, amino metabolism of liver in weaned piglets undergoing feed transition and then challenged with lipopolysaccharide

Provisionally accepted
Can Yang Can Yang 1,2*Xiaowu Tang Xiaowu Tang 3Wang Min Wang Min 4Han Yang Han Yang 2Huansheng Yang Huansheng Yang 4Yancan Wang Yancan Wang 4Yulong Yin Yulong Yin 5
  • 1 College of Life Sciences and Environment, Hengyang Normal University, Hengyang, China
  • 2 Hengyang Normal University, Hengyang, Hunan Province, China
  • 3 Hunan Polytechnic College of Environment and Biology, Hengyang, Hunan Province, China
  • 4 Hunan Normal University, Changsha, Hunan Province, China
  • 5 Institute of Subtropical Agriculture, Chinese Academy of Sciences (CAS), Changsha, China

The final, formatted version of the article will be published soon.

    To find out whether dietary amylose/ amylopectin ratio (DAR) could attenuate injury in lipopolysaccharide (LPS)-challenged piglets, sixty male weaned piglets (Duroc × Landrace × Yorkshire, 21 days old, 6.51 ± 0.64 kg) were allotted to 5 dietary treatments with 12 cages per treatment, and fed ad libitum with diets different in DAR (0.00, 0.20, 0.40, 0.60 and 0.80). Feed transformation occurred from D15 to D21. On day 28, 12 h before slaughter, pigs were intraperitoneal injected with 100 μg/kg body weight LPS or sterile saline. Results showed that LPS stress caused an increase in serum urea nitrogen (UREA) and triglyceride (TG), but a decrease in alanine aminotransferase (ALT) activity and glucose (GLU) concentration (P < 0.05). Serum immunoglobulin G (IgG) concentration increased in DAR 0.80 but decreased in other groups after LPS stress (P < 0.05). Compared with the control group, concentrations of Ile, Leu, Phe, Val, Thr, Arg decreased in serum but increased in liver after LPS stress (P < 0.05).Serum Arg, Tyr, Sar, Ans, Orn increased linearly with increasing DAR (P < 0.05).Piglets in diet DAR 0.00 had highest superoxide dismutase (SOD1) and glutathione peroxidase 1 (GPX1) mRNA expression in liver than those in other groups (P < 0.05). There was significant effect of LPS stress * dietary DAR on total SOD activity and SOD1 mRNA gene expression (P < 0.05), LPS stress caused an increase in those two indices for pigs in groups 0.00 and 0.80. Piglets in diet 0.80 had the highest hepatic Cu, Fe, Mn, Zn concentrations than those in other groups (P < 0.05). Cecal indol(e) concentration was higher in diet 0.00 than that in diet 0.80 (P < 0.05). After LPS stress, colonic skatole concentration increased in DAR 0.40, 0.80 but decreased in other groups (P < 0.05). In conclusion, adverse effects of the LPS challenge could be reversed by feeding weaned piglets with low or high DAR diet through regulating amino metabolism and antioxidant function.

    Keywords: dietary amylose/amylopectin ratio, LPS stress, Amino acid, antioxidant activity, Weaned piglet

    Received: 19 May 2024; Accepted: 12 Dec 2024.

    Copyright: © 2024 Yang, Tang, Min, Yang, Yang, Wang and Yin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Can Yang, College of Life Sciences and Environment, Hengyang Normal University, Hengyang, China

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