Chiara Di Berardino1*
Urte Barceviciute1
Chiara Camerano Spelta Rapini1
Alessia Peserico1
Giulia Capacchietti1
Nicola Bernabò1,2
Valentina Russo1
Valentina Gatta3,4
Fani Konstantinidou3,4
Marisa Donato1,3,4
Barbara Barboni1- 1Department of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Teramo, Italy
- 2Institute of Biochemistry and Cell Biology (IBBC), National Research Council, Rome, Italy
- 3Department of Psychological Health and Territorial Sciences, School of Medicine and Health Sciences, “G. d'Annunzio” University of Chieti-Pescara, Chieti, Italy
- 4Unit of Molecular Genetics, Center for Advanced Studies and Technology (CAST), “G. d'Annunzio” University of Chieti-Pescara, Chieti, Italy
Introduction: Excessive calorie intake poses a significant threat to female fertility, leading to hormonal imbalances and reproductive challenges. Overconsumption of unhealthy fats exacerbates ovarian dysfunction, with an overproduction of reactive oxygen species causing oxidative stress, impairing ovarian follicle development and leading to irregular ovulation and premature ovarian failure. Interest in biological matrices with high antioxidant properties to combat diet-related oxidative stress has grown, as they contain various bioactive factors crucial for neutralizing free radicals potentially preventing female reproductive health. This systematic review evaluates the female reproductive impact of biological matrices in mitigating oxidative damages induced by over calory habits and, in particular, high fat diets.
Methods: A comparative approach among mammalian models was utilized to interpret literature available data. This approach specifically investigates the antioxidant mechanisms of biological matrices on early and late ovarian folliculogenesis, under physiological and hormone-induced female reproductive cycle. Adhering to the PRISMA 2020 guidelines, only English-language publications from peer-reviewed international indexes were considered.
Results: The analysis of 121 publications meeting the inclusion criteria facilitated the identification of crucial components of biological matrices. These components, including carbocyclic sugars, phytonutrients, organosulfur compounds, and vitamins, were evaluated for their impact on ovarian follicle resilience, oocyte quality, and reproductive lifespan. The detrimental effects of oxidative stress on female fertility, particularly exacerbated by high saturated fat diets, are well-documented. In vivo studies across mammalian preclinical models have underscored the potential of antioxidants derived from biological matrices to mitigate diet-induced conditions. These antioxidants enhance steroidogenesis and ovarian follicle development, thereby improving oocyte quality. Additionally, discussions within these publications emphasized the clinical significance of these biological matrices, translating research findings into practical applications for female health.
Conclusion: Further research is essential to fully exploit the potential of these matrices in enhancing female reproduction and mitigating the effects of diets rich in fatty acids. This requires intensified in vitro studies and comprehensive collection of in vivo data before clinical trials. The promotion of ovarian resilience offers promising avenues for enhancing understanding and advancing female reproductive health world-wide.
1 Introduction
Dietary choices, with an excessive calorie intake, present substantial threat to female fertility by triggering hormonal imbalances giving rise to reproductive challenges (1–4). Although fatty acids represent essential components of the diet and exert a key role in the body metabolic processes, excessive consumption of unhealthy fats, particularly saturated and trans fats, can potentially contribute to ovarian dysfunction and failure-related pathological conditions (5–8). Going into detail, the dietary-related pathological condition involves an excess production of reactive oxygen species (ROS), resulting in oxidative stress that can harm the synergistic development of somatic and germinal-ovarian follicle compartments, leading to irregular ovulation patterns and premature ovarian failure (9, 10). In this context, diet induced-ROS production can impede vasodilation and blood flow to reproductive organs, disrupting the functioning of the hypothalamic-pituitary-ovarian axis and contributing to (1) impact the reproductive hormones regulation (11–13), (2) induce insulin resistance and hyperleptinemia (14), (3) promote chronic low-grade inflammation (15, 16), (4) affect oocyte quality (13, 17) and (5) influence the maternal uterine environment (18), making it less receptive to embryo implantation and the maintenance of pregnancy (19, 20). To counteract the overall detrimental effects on female reproductive health, the use of antioxidants to mitigate, and potentially reverse, the oxidative stress associated with a high-fat diet could prove to be a beneficial strategy (13, 18, 19, 21–25).
In recent years, research on biological matrices with high antioxidant properties, has garnered significant interest due to the growing recognition of the importance of this compound in promoting health and preventing oxidative stress related to an unbalanced diet habit (26–28). More in detail, biological matrices, which can have either plant or animal origins, often contain a variety of antioxidant compounds such as vitamins, polyphenols, flavonoids, and carotenoids, playing a crucial role in mitigating the oxidative damage caused by free radicals in the body. Literature studies have demonstrated that a diet rich in foods containing these biological matrices can help reduce the risk of oxidative-related conditions, supporting healthy ovarian follicle growth and overall reproductive health (29).
Considering that the interest in these biological matrices stems from the growing awareness of the importance of a diet rich in antioxidants for promoting female reproductive wellbeing, the aim of this systematic review is to assess the impact of biological matrices on controlling oxidative exposure due to a high caloric food intake and, in turn, its potential influence on ovarian function. The employment of a comparative approach amongst mammalian models during folliculogenesis has been proposed for interpreting the vast quantity of data gathered so far. Specifically, it focuses on the study of antioxidant mechanisms of action of biological matrices and their beneficial properties in relation to the physiological processes associated with early and late ovarian folliculogenesis, either under physiological or hormone-induced conditions.
2 Methods
2.1 Bibliographic search methods
The present systematic review was carried out following the Preferred Reporting Items for Systematic Review and meta-analysis (PRISMA) Statement 2020 Checklist Guidelines (http://www.prisma-statement.org/) (30).
Scientific literature published in the peer-reviewed international index was considered. Specifically:
• Advanced Search of Web of Science [v.5.35] “Core collection” archive (https://apps.webofknowledge.com/WOS_AdvancedSearch).
• PubMed Advanced Search Builder archive (https://pubmed.ncbi.nlm.nih.gov/advanced/) was considered.
“TS” was used as a Field tag, “AND,” “OR,” and “NOT” were used as Boolean operators.
The keywords were combined to elaborate the main paragraphs, as follow:
• List: “WOS Advanced Search”
((((TS = (oxidative stress)) AND TS = (biological antioxidant*)) AND TS = (female reproduction)) AND TS = (mammal*)) NOT TS = (male)
(TS = (oxidative stress)) AND TS = (ovarian failure)
(TS = (antioxidant*)) AND TS = (ovarian folliculogenesis)
((TS = (high fat diet)) AND TS = (antioxidant)) AND TS = (ovary)
(TS = (biological antioxidant)) AND TS = (ovary)
(TS = (biological matrix)) AND TS = (ovarian damage)
(TS = (biological matrix*)) AND TS = (ovarian folliculogenesis)
(TS = (diet)) AND TS = (female infertility)
Matched list: 925 publications.
• List: “PubMed Advanced Search”
((((oxidative stress) AND (high fat diet)) AND (female reproduction)) AND (mammal*)) NOT (male)
(folliculogenesis) AND (high fat diet)
((fertility) AND (high fat diet)) AND (antioxidant*) NOT (male)
(((ovarian follicle*) AND (high fat diet)) AND (antioxidant*)) NOT (male)
(((high fat diet) AND (antioxidant)) AND (follicle*)) NOT (male)
((high fat diet) AND (oxidative stress)) AND (ovarian follicle*)
(((high fat diet) AND (follicle*)) AND (oxidative stress)) NOT (male)
(antioxidant*) AND (folliculogenesis) AND (fat)
(antioxidant*) AND (folliculogenesis) AND (obese) AND (mammal)
(antioxidant*) AND (ovarian follicle) AND (obese) AND (mammal) NOT (male)
Matched list: 80 publications.
2.2 Eligibility criteria
Only English-language publications were considered. The reviewed articles, which investigated the connection between high-fat diets, ROS production, and female fertility in relation to follicle development, steroid production, and oogenesis, were published from 1971 to 2024. The initial screening of studies was primarily based on their titles and accompanying abstracts. Except for articles focused on obesity, research papers that did not explicitly address the topics of “high-fat diet” and “oxidative stress” related to fertility were excluded. Studies involving non-mammalian species or male animal models were ultimately removed from consideration.
2.3 Study selection
Considering the information provided above, each list was subsequently cross-referenced, and the initial count of titles, in accordance with the search keywords, was estimated to be 1,005. Duplicate were eliminated. Comprehensive manuscripts were then obtained for all the chosen documents, and the final selection was made after a thorough evaluation.
3 Results
Original research articles (n = 40), meta-analysis (n = 3), comparative studies (n = 2), observational studies (n = 1), editorial (n = 2), randomized controlled trials (n = 5) and clinical trials (n = 1) were chosen to investigate the impact of diet induced-ROS production on specific mammalian models and to evaluate their reproductive outcomes. Review articles (n = 67) were included solely for the purpose of enhancing and discussing the gathered data. Their reference lists were also examined and analyzed, alongside a comprehensive website check, to identify other studies or potentially related information that could be further included in this review. Ultimately, 121 publications conformed to the inclusion criteria (Figure 1). As a note, in the systematic selection of the aforementioned articles, the reference related to the proper utilization of the PRISMA Statement 2020 Checklist Guidelines (30) is excluded from the count.
Figure 1. PRISMA flow diagram. The diagram shows the systematic process adopted to include papers captured by the literature search. Preferred Reporting Items for Systematic Review and meta-analysis” (PRISMA) Statement 2020 Checklist Guidelines were followed. Image created with Biorender.com.
3.1 Biological matrices-derived antioxidants as dietary components: their impact on ovarian health and their role in modulating the intricate relationship between high-fat diet-induced oxidative stress and ovarian function
The intricate relationship between high-fat diet (HFD)-induced oxidative stress and ovarian function has profound implications for the ovarian microenvironment (4, 31). The ovarian microenvironment is a complex and dynamic milieu within the ovaries that plays a crucial role in supporting normal ovarian function and reproductive health (32). It encompasses the cellular, biochemical, and physical factors that influence the growth and health of ovarian follicles and their enclosed oocytes (33). Maintaining the integrity of the ovarian microenvironment is essential for a proper hormonal regulation (34), follicular development (35) and oocyte maturation (36), mitochondrial function within the oocytes (37), vascularization and blood supply (38–41), immunological regulation (42), as well as fertility and reproductive potential (33, 43, 44). However, the disruptions caused by oxidative stress can have far-reaching consequences on the ovarian microenvironment, impacting the overall mechanisms regulating ovarian function and female reproductive health (45, 46). More in detail, in a healthy organism, ROS and antioxidants remain in balance. When the balance is disrupted toward an overabundance of ROS due to a high levels of fat intake, oxidative stress occurs, determining metabolic dysfunctions that can impair the entire reproductive span of female's life (45, 47). Cells have developed a wide range of antioxidants systems to limit production of ROS, inactivate them and repair cell damage (48–50). These systems are based on the use of enzymatic and non-enzymatic antioxidant systems to protect against ROS and subsequent damage to membranes and macromolecules. Despite the presence of endogenous enzymatic antioxidants, composed of superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase, that catalyze reactions to neutralize free radicals (4, 5), nowadays the role and the biological action of biological matrices as exogenous antioxidants are under investigation, considering the potential role of these dietary supplements in help reverse or mitigate the effects of HFD induced-oxidative stress on ovarian health (51–53).
More in detail, biological matrices that have a positive influence on female reproductive health include carbocyclic sugar (54), phytonutrients (55–57), organosulfur compound (54, 58), hormones (59, 60), neuropeptide (61), organic acids (62), and vitamins (58). On the contrary, components exerting a negative influence mostly include high glycemic index carbohydrates, large amount of animal protein, saturated fatty acids, and trans fatty acids, which are typical of the Western model of nutrition, which is closely related to increased oxidative stress (4, 5, 53).
The research on the role of diet and other modifiable factors associated with lifestyle in the prophylaxis of fertility disorders is still ongoing. However, few studies have established a direct link between nutrition and the risk of anovulatory infertility. Therefore, the aim of the following sections was to summarize available knowledge about diet and its relation to anovulatory infertility and indicate avenues for future research. Considering this, the following paragraphs aim to provide a comprehensive analysis across mammal species of the impact that a balanced diet supplemented with antioxidants derived from biological matrices can have on female reproductive health. These sections also endeavor to gather crucial information from the literature regarding the mechanisms by which biological matrices counteract the detrimental effects of a diet rich in fatty acids on female reproductive wellbeing, with a particular emphasis on ovarian follicle development, oocyte quality, and reproductive lifespan.
A comprehensive overview delineating the effects of a balanced diet enriched with biologically derived antioxidants to mitigate the adverse impact of HFD on female reproductive health across mammal species was depicted in Supplementary Figure 1.
3.1.1 Biological matrices-derived antioxidants in HFD-induced oxidative stress: implications for ovarian follicle development
Folliculogenesis allows primordial germ cells mature within follicles into oocytes capable of fertilization and embryonic development, which is crucial for reproductive success (63). Ovarian follicle development, which is critical part of female reproductive health, is increasingly recognized as susceptible to dietary influences, one of which is causing oxidative stress (4). In fact, HFD has been found to result in an excessive availability of energetic precursors, such as glucose, pyruvate, and other metabolic intermediates. Additionally, it leads to the accumulation of intracellular lipids that bind to proteins, forming lipid droplets, thus causing lipotoxicity (4, 63). These precursors are metabolized through the glycolytic pathway and the respiratory chain to produce energy in the form of ATP, which in turn causes impairment in ovarian follicle development (64). The accumulation of intracellular lipids leads to high levels of free fatty acids, which are susceptible to oxidative damage and the formation of cytotoxic and highly reactive lipid peroxides. Ultimately, these compounds are detrimental to intracellular organelles, particularly the endoplasmic reticulum (ER) and mitochondria (3, 5). Thus, HFD can cause oxidative stress on the ovary, specifically targeting follicle development, survival, and hormone production essential for regulating folliculogenesis, which in turn negatively impacts female fertility (5).
There are several pathways by which oxidative stress inflicts damage, such as reactive oxygen species (ROS) formation, diminished antioxidant capacity in the ovary, disrupted mitochondrial function and several others reported in recent literature outlined in Table 1 with further details available in Supplementary Table 1. The oxidative stress profoundly impacts not only follicle formation, growth, and maturation, but also oocyte quality, potentially compromising embryo development (64). The results described in Table 1 demonstrate that while under normal physiological and hormonal conditions follicles progress to yield healthy oocytes, oxidative stress induced by HFD can disrupt this process at any point, impairing steroidogenesis in addition to affecting folliculogenesis as well as oocyte quality, and therefore reproductive lifespan.
Table 1. An overview of the current literature survey on antioxidant treatments for HFD-induced oxidative stress on ovarian function in mammalian models.
The studies summarized in Table 1 demonstrate encouraging results of antioxidant dietary supplementation in ameliorating HFD-induced damage, ranging from moderately restoring to significantly improving folliculogenesis compared to control groups. The existing literature highlights the potential of antioxidant treatments in relieving HFD-induced oxidative stress, pointing toward future research directions and treatment possibilities that hinge on a more comprehensive understanding of oxidative stress damage and pathways by which it occurs.
Most of the existing studies on this topic predominantly present results from in vivo animal models, primarily rodents such as rats or mice, with limited experiments conducted in vitro. Moreover, many studies focus on HFD induced oxidative stress or oxidative stress effects on general fertility, but do not specify the combination of these topics to specifically report the effects of HFD induced oxidative stress on female fertility. This limited in vitro experimentation poses challenges in definitively attributing improved outcomes to specific treatments as folliculogenesis is a very complex process affected by numerous factors. More distinct and targeted studies in different animal models or even human trials would provide a more comprehensive view of the effects of HFD induced stress on female fertility. Additionally, performing experiments in vitro would allow for a more controlled experiment setting, which would in turn lend credibility and integrity to the results.
A detailed summary of the current section was provided in Figure 2.
Figure 2. Overview of bibliographic research on the use of biological matrices as antioxidants to counteract the detrimental effect of a High-Fat Diet (HFD) on ovarian folliculogenesis and overall female reproductive health across mammal species. (A) Percentage (%) of animal models used for in vivo and/or in vitro studies. Percentages are calculated based on the literature information reported in Supplementary Table 1. (B) Percentage (%) of bioactive compound categories. Percentages are calculated based on the literature information reported in Supplementary Table 1. Image created with Biorender.com.
3.1.2 Effect on early ovarian folliculogenesis
HFD induced oxidative stress causes significant challenges on early ovarian folliculogenesis, especially on follicle growth and survival. However, various studies have elucidated strategies to mitigate these effects using antioxidants as dietary supplements in various animal models which are summarized in Table 1. For example, El-Sayyad et al. observed a significant preservation of ovarian follicles under conditions of oxidative stress induced by a lipid-rich diet. This preservation was accompanied by an increase in the proliferation and development of both primordial and primary follicles, and a restoration of ovarian stroma to near-normal conditions. These effects were achieved through the in vivo administration of a diet containing a combination of two phytonutrients—barley (Hordeum vulgare) and dates (Phoenix dactylifera)—to rodent models (55). Furthermore, the study noted an enhancement in the levels of antioxidant enzymes, effectively mitigating oxidative stress and lipid peroxidation within the ovary. Similarly, other studies, including those by Majd et al. (57) and Shoaib et al. (66), reported enhanced growth of early-stage follicles and a reduction in the rate of atretic follicles. These positive outcomes were attributed to the heightened activities of flavonoids and phenolic-antioxidant properties found in Okra (Abelmoschus esculentus) (57), ferulic acid, kaempferol, malvidin, caffeoylquinic acid, and quercetin derivatives (Centratherum anthelminticum) (66). These dietary compounds were administered in vivo in a rat model, resulting in diminished lipid peroxidation levels.
Moreover, the incorporation of thymoquinone into the diet of mouse models (67) led to the activation of genes associated with the AMPK/PGC1α/SIRT1 pathway. This pathway appears to play a pivotal role in enhancing oxidative status, reducing inflammatory markers in HFD conditions, and boosting mitochondrial function. This ultimately resulted in an increased count of early-stage follicles that were able to reach the stage of Graafian follicles, allowing them to ovulate meiotically competent oocytes (67). Additionally, interventions that target kisspeptin expression via the dietary supplement of apple vinegar in rodent in vivo model (62), and those that target serotonin-melatonin synthesis related to gut microbiota in porcine model used as dietary supplements (60), have significantly contributed to the protection against follicular atresia and the promotion of early ovarian folliculogenesis.
Furthermore, numerous studies have pinpointed theca cells and granulosa cells as the primary sites for antioxidant action. For example, leptin treatment in mouse model resulted in a significant expansion of the granulosa and theca cell layers, coupled with increased LHR immunoreactivity (74). This not only accelerated follicular turnover but also boosted the number of early-stage follicles capable of reaching the antral stage.
Concurrently, the incorporation of the neuropeptide phoenixin into the diet of rodents resulted in observable changes, including reduced ovarian weights, decreased peri-ovarian fat pads, and modulated luteinizing hormone (LH) receptor positivity. This dietary intervention also led to a reduction in apoptosis and inflammation within the ovary (61). In a parallel vein, research conducted by Zhuo et al. (60) demonstrated a significant decrease in granulosa cell apoptosis and the proportion of atretic antral follicles. This outcome was partially attributed to the synthesis of serotonin-melatonin related to gut microbiota, which ultimately led to a reduction in atretic follicles and a decrease in the expression of apoptotic markers within the ovaries. Furthermore, the observation of weak positive reactions of caspase-3 and iNOS in the granulosa cells of ovarian follicles and stromal cells suggests an amelioration of the adverse effects of a HFD on reproductive organs, as referenced. This improvement is characterized by an increased count of early-stage follicles and a reduction in the number of cysts following the administration of betalain (71) and frankincense (Boswellia Carterii) (72) in a rodent model diet. These comprehensive findings underscore the complex interplay of various pathways and interventions in mitigating the impacts of high-fat-diet-induced oxidative stress on ovarian folliculogenesis, offering promising avenues for future therapeutic strategies.
3.1.3 Effects on late ovarian folliculogenesis under physiological and hormonal-induced conditions
The detrimental impacts of a HFD on late ovarian folliculogenesis under various physiological and hormonal-induced conditions, such as obesity, polycystic ovary syndrome (PCOS), insulinemia, and metabolic syndrome, are substantial. However, research suggests promising avenues for mitigating these effects. For instance, high-fat diet-induced oxidative stress can lead to degenerative changes in the ovary, while antioxidant supplementation in the diet is associated with increased activities of antioxidant enzymes and protection against degenerative alterations, consequently improving PCOS symptoms and restoring reproductive hormone balance (65, 66).
Moreover, Nilsson et al. employed a multi-antioxidant supplement, including organosulfur compound (α-lipoic acid), phytonutrients (forskolin, extracts of green coffee bean, green tea, and beetroot), vitamin E (α-tocopheryl acetate), and Coenzyme Q10 (CoQ10), in an obese rat model. Their study demonstrated promising results in mitigating obesity-induced infertility by reducing ovarian inflammation and follicular atresia, consequently enhancing reproductive success (58). Moreover, targeted treatments such as MitoQ10 supplementation (68) and melatonin supplementation acting via the SIRT3–SOD2-dependent mechanism (59) demonstrate the potential to reverse endocrine abnormalities, reduce oxidative stress, and improve mitochondrial function, consequently ameliorating obesity-induced reproductive defects and promoting oocyte developmental potential. Additionally, modulation of hormonal levels, including FSH, LH, estrogen, and progesterone, plays a crucial role in restoring ovarian function. Studies conducted by Basha et al. (61) and Shams et al. (62) suggests that the hormonal imbalances caused by a HFD can be rectified in rat models through the administration of a diet containing apple vinegar and phoenixin. This dietary intervention appears to enhance folliculogenesis and bolster antioxidant responses within the ovaries. Collectively, these findings show the intricate interplay between high-fat diet-induced oxidative stress, hormonal imbalances, and ovarian folliculogenesis, while offering insights into potential therapeutic interventions to mitigate these adverse effects and restore reproductive health.
3.1.4 Biological matrices-derived antioxidants in HFD-induced oxidative stress: implications for oocyte quality
The harmful effects of a high-fat diet on oocyte quality, particularly concerning spindle formation, chromosome alignment, meiotic defects, mitochondrial function, and DNA damage, pose significant challenges to reproductive health. Studies elucidate the intricate interplay between high-fat diet-induced oxidative stress and various aspects of oocyte quality. For instance, HFD-induced oxidative stress can impair oocyte spindle formation and chromosome alignment, leading to abnormal meiotic events (56, 59). However, interventions targeting mitochondrial function, such as phoenixin administration (61), MitoQ10 (68), and resveratrol supplementation (56), show promise in mitigating these effects. They regulate mitochondrial dynamics, enhance ovarian cell survival, and reduce apoptotic markers. Ultimately, they improve oocyte evolutionary and meiotic competence acquisition by lowering levels of ROS and lipid droplets, and by controlling mitochondrial distribution. Additionally, they contribute to reverse negative effects such as abnormal spindle morphology and chromosome arrangements in oocytes.
Notably, HFD is known to induce oxidative stress, which is implicated in DNA damage within oocytes. However, a study conducted by Shoaib et al. (66) provides promising evidence that a supplemental diet containing ferulic acid, kaempferol, malvidin, caffeoylquinic acid, and quercetin derivatives (C. anthelminticum) can effectively counteract these adverse effects. Specifically, this supplemental diet, when administered to rat model, has been shown to increase the activity of antioxidant enzymes such as catalase and superoxide dismutase (66). This, coupled with higher levels of glutathione and lower levels of malondialdehyde, plays a crucial role in protecting oocytes against DNA damage caused by reactive oxygen species (ROS), thereby reversing the harmful effects of the HFD (66). Similarly, interventions like MitoQ10 supplementation demonstrate efficacy in reducing cellular oxidative stress and improving mitochondrial function, thus mitigating DNA damage and preserving oocyte quality (68). Moreover, the influence of a HFD on the rigidity of the zona pellucida in oocytes within Graafian follicles is significant. Jia et al. have shown that targeted interventions can markedly enhance the rigidity of the zona pellucida. This improvement not only boosts oocyte meiosis but also mitigates oxidative stress, ultimately leading to the restoration of the oocytes' mechanical properties (56). These findings underscore the multifaceted impacts of HFD-induced oxidative stress on oocyte quality and highlight the importance of targeted interventions to ameliorate these effects, thereby preserving the oocyte quality and developmental potential.
3.1.5 Biological matrices-derived antioxidants in HFD-induced oxidative stress: implications for reproductive lifespan
HFD-induced oxidative stress profoundly impacts reproductive lifespan, with diverse effects ranging from cyst formation to reproductive success. Cyst formation, a common consequence of HFD consumption, is mitigated by antioxidant interventions targeting oxidative stress which then demonstrate a decrease in the formation of follicular cysts, improving the reproductive potential (71, 72). Moreover, interventions aimed at reducing oxidative stress have shown promise in lowering the number of atretic follicles, inflammation, and apoptosis, thus ameliorating ovarian function and reproductive outcomes (56, 74).
Moreover, it is crucial to note that oxidative stress induced by a HFD has a significant impact on both the litter size and the survival rates of offspring. However, a study conducted by Wen et al. has shown that targeted interventions can effectively counteract these adverse effects. Specifically, the administration of phycocyanin into the mouse diet has been demonstrated to not only increase litter size but also improve offspring survival rates (70). This improvement is achieved by reducing follicular atresia and enhancing the levels of antioxidant enzymes in the ovaries, thereby mitigating the harmful effects of HFD-induced oxidative stress (70).
It is important to emphasize that the detrimental effects of obesity resulting from a HFD, can lead to infertility. However, in scenarios of obesity-induced infertility, improved reproductive success has been observed. This improvement has been achieved in a study conducted by Nilsson et al. (58) through interventions with organosulfur compounds such as α-lipoic acid, phytonutrients including forskolin and extracts of green coffee bean, green tea, and beetroot, vitamin E in the form of α-tocopheryl acetate, and Coenzyme Q10 (CoQ10) (58, 73). These interventions were applied in an obese rat model, specifically targeting oxidative stress, and led to significantly higher rates of reproductive success (58). These findings highlight the critical role of mitigating oxidative stress in enhancing fertility outcomes and ensuring the survival of offspring.
Furthermore, HFD-induced oxidative stress disrupts the ovarian cycle, impacting ovulation and subsequent successful fertilization and pregnancy rates. However, evidence in literature has shown that interventions targeting oxidative stress with ferulic acid, kaempferol, malvidin, caffeoylquinic acid, and quercetin derivatives (C. anthelminticum) (66) as well as the combined use of myo-inositol and α-lipoic acid (54), the diet administration of Gum Arabic (Acacia senegal) (65) and flavonoids like luteolin (69) hold promise in restoring normal ovarian cycle patterns (54, 66) and reduced ovarian degenerative changes (65) in rodent models, thus improving reproductive success in terms of fertilization and pregnancy rates (66, 69).
Overall, oxidative stress, induced by a HFD, has a profound impact on the reproductive lifespan, with inflammation and atresia being major concerns. Research has highlighted several antioxidant interventions that effectively mitigate these issues, thereby improving ovarian function and reproductive outcomes (56, 74). These interventions lead to a reduction in the number of atretic follicles, a decrease in ovarian inflammation, and a reduction in apoptosis. This is evidenced by decreased ovarian weights, reduced peri-ovarian fat pads, and modulated LH receptor positivity. Furthermore, the successful mitigation of inflammation and atresia is linked with improved reproductive success in obesity cases resulting from HFD. This underscores the critical importance of addressing oxidative stress in order to preserve ovarian health and fertility.
3.2 Nurturing ovarian resilience: perspectives in leveraging biological matrices as antioxidants amidst high-fat diets for female's reproductive wellbeing
3.2.1 Clinical relevance: translating findings into female's health
The concept of nurturing ovarian resilience using biological matrices as antioxidants amidst HFD holds significant clinical relevance for female's reproductive health (75).
HFD have been associated with oxidative stress and inflammation, leading to impaired ovarian function and accelerated ovarian aging. Furthermore, oxidative stress and inflammation often interact synergistically, creating a vicious cycle that exacerbates ovarian dysfunction and compromises reproductive outcomes. ROS can stimulate inflammatory signaling pathways, while inflammatory mediators can induce the production of ROS, amplifying oxidative stress and inflammation in the ovaries. This crosstalk between oxidative stress and inflammation further contributes to the pathogenesis of ovarian disorders associated with HFD (4, 5). This results in reduced oocyte quantity and quality, negatively affecting fertility (29, 76, 77). Assisted reproductive technology (ART), developed in the late 20th century, has become a therapeutic solution to infertility issues (78). However, while ART can address the symptoms of fertility decline, it does not target the underlying cause related to HFD consumption.
This aspect is significant, as clinical studies have established a connection between the consumption of a HFD in women of reproductive age and the onset of conditions like endometriosis (79–83) and polycystic ovary syndrome (PCOS) (84–87). Both of these conditions can result in a diminished ovarian reserve and are among the primary contributors to infertility in women (88, 89). This identifies the urgent need to thoroughly study the ways in which HFD affects ovarian resilience and the overall reproductive system in order to identify the most suitable strategies to address it (78). Going into detail, endometriosis is a hormone-related, chronic inflammatory condition affecting women of childbearing age (90, 91). Despite ongoing research, the etiology and pathogenesis of endometriosis remain incompletely understood. Unlike some lifestyle diseases, the association between nutrition and the pathogenesis of endometriosis has been investigated and confirmed (90, 91). In a study conducted in 2018, Yamamoto et al. (79) found that prolonged consumption of saturated fats was associated with a 56% higher risk of developing endometriosis compared to those who are not accustomed to consuming them. Furthermore, Missmer et al. (80) demonstrated that women who regularly consumed trans fats were 48% more likely to develop endometriosis compared to those who did not. Although current research does not provide a clear dietary guideline or scientific recommendation for conditions related to endometriosis, it has been noted that omega-3 fatty acids, especially when combined with vitamin B12, can effectively alleviate symptoms related to endometriosis, particularly dysmenorrhea (81, 82). Furthermore, randomized controlled trials (RCTs) have demonstrated that in vivo administration of vitamins C and E resulted in reduced pelvic pain and lowered levels of inflammatory markers in peritoneal fluid compared to untreated patients. However, any beneficial effect of in vivo administration of vitamin C in patients with endometriosis did not positively impact on the in vitro fertilization (IVF) outcomes (83).
Regarding PCOS, it often presents with symptoms such as menstrual irregularities, absence of menstruation, and elevated androgen levels, along with associated metabolic dysfunctions (92–95). HFD have been linked to the development of metabolic disorders associated with PCOS and weight gain. This intensifies obesity and disrupts the hypothalamic-pituitary-ovarian axis through high adipokine production, causing impaired GnRH pulse due to overstimulation of kisspeptin neurons (5). At the pituitary level, there is abnormal release of gonadotropins, reflected locally in increased androgen production, which ovarian cells fail to fully convert into estrogen (92–95). This disruption triggers a cascade of events leading to increased insulin resistance, hyperinsulinemia, and the release of inflammatory adipokines. These factors contribute to an increase in fat synthesis and a decrease in fat breakdown, further exacerbating the metabolic and reproductive complications of PCOS (75, 92–95). Furthermore, PCOS patients typically exhibit heightened markers of oxidative stress in follicular fluid, reduced quality of oocytes and embryos (84), and elevated levels of chronic inflammation markers (85). While data on the efficacy of antioxidants to alleviate oxidative stress for enhancing fertility in PCOS patients remain limited (86), the administration of biological matrices combination comprising vitamin A, vitamin B1, vitamin B6, vitamin B12, vitamin C, vitamin D3, vitamin E, nicotinamide, and folic acid has shown promising results in improving pregnancy rates (87). Furthermore, two RCTs performed on patients with PCOS revealed that in vivo antioxidant treatment (specifically, resveratrol) improved the quality of oocytes and embryos (87). Additionally, treatments with vitamins D and E, respectively increased the rates of implantation and overall pregnancy success (96).
Oxidative stress induced by HFD seems to be linked with the development of hypertensive and metabolic disorders during pregnancy (97). A mild level of oxidative stress is a natural maternal response to pregnancy, due to the increased metabolic activity in the placental mitochondria, which is related to the growing fetus's higher metabolic demand (98). However, when the pregnancy-associated oxidative stress exceeds, it becomes detrimental to the pregnancy, creating conditions that can harm both the mother and the fetus (99). The pathological conditions caused by excessive oxidative stress during pregnancy encompass a range of hypertensive disorders, including hypertension in pregnancy, chronic hypertension, white-coat hypertension, masked hypertension, gestational hypertension, preeclampsia, proteinuria, and hemolysis with elevated liver enzymes and low platelet count (100). Furthermore, oxidative stress is implicated in the pathogenesis of gestational diabetes mellitus (101, 102). Clinical experience in using exogenous biological matrices as antioxidants for preventing and treating pregnancy complications is still in its early stages. One promising area of research is the potential use of melatonin in treating preeclampsia (103, 104). This interest is sparked by findings that lower melatonin levels are linked to the onset of this condition (105). In fact, studies have shown that administering melatonin to women suffering from severe early onset preeclampsia can significantly reduce oxidative damage to the maternal endothelium and extend the duration of pregnancy (106). Other antioxidants, such as vitamin C, vitamin E, and resveratrol, have been used in the management of preeclampsia. However, two large RCTs that tested vitamins C and E (107, 108) did not show any significant improvements. On the other hand, resveratrol showed promising results. It was found to be an effective supplement to oral nifedipine treatment for controlling blood pressure in women with preeclampsia (109, 110). Given these considerations, utilizing biological matrices as antioxidants represents a promising strategy to mitigate oxidative stress and enhance ovarian resilience. Translating these discoveries into clinical practice has the potential to revolutionize women's health. Strengthening ovarian resilience could extend women's reproductive lifespan, ultimately enhancing their overall wellbeing and quality of life. Furthermore, this approach could provide more control over reproductive health decisions, potentially leading to the development of personalized medicine strategies that consider individual variations in diet, lifestyle, and genetic factors.
In summary, the prospect of fortifying ovarian resilience using biological matrices as antioxidants in the context of HFD holds significant promise for advancing women's reproductive health. However, comprehensive research is essential to fully understand the underlying mechanisms and to effectively translate these findings into clinical interventions.
3.2.2 Future directions: uncharted territories in understanding ovarian health
The concept of fostering ovarian resilience through the utilization of biological matrices as antioxidants, particularly in the context of a diet rich in fat, introduces new and unexplored avenues in the comprehensive understanding of ovarian health. As progress continues in this field, there are four key areas that warrant further investigation (111).
Firstly, the specific mechanisms by which an HFD induces oxidative stress and subsequently impacts ovarian function require thorough elucidation (76). This could entail a detailed examination of the role of specific dietary components, the influence of gut microbiota, and the intricate interaction between diet, inflammation, and hormonal regulation (112). Understanding these mechanisms will provide a foundation for developing targeted interventions to mitigate the adverse effects of an HFD on stepwise ovarian-mediated mechanisms (113, 114).
Secondly, while the potential of biological matrices as antioxidants is promising, additional research is necessary to identify the most effective matrices and comprehend their interaction with ovarian tissues (115). This could involve investigating a wide variety of biological matrices, ranging from vitamins (87) and minerals to plant extracts (116) and probiotics (117). The goal is to identify those matrices that not only have potent antioxidant properties but also interact synergistically with ovarian cells to enhance their resilience (118–120). In fact, the in vitro approach is indispensable for gathering data on potential impacts on the somatic and germinal compartments during development. This approach facilitates tracking of the effects induced on the quality of the egg cell throughout the initial stages of embryonic development. Notably, the use of 3D models in culture enables the extension of these observations to the development of embryos in the pre-implantation phase. Moreover, the employment of 3D culture models allows for the analysis of events occurring during the initial stages of ovarian folliculogenesis, and the tracking of their effects, from the gonadotropin-independent-sensitive phase to the gonadotropin-dependent phase.
Thirdly, before advancing to clinical trials, it is possible to collect a wealth of in vivo data from preclinical models. Ideally, these models should be developed on medium-sized monogastric mammals, as this closely mirrors the pathway of Non-Esterified Fatty Acids (NEFA) utilization in humans. The goal is not only to demonstrate the effectiveness of these interventions through rigorous clinical trials but also to understand how to seamlessly integrate them into existing treatment protocols and lifestyle recommendations (75). It is crucial to ensure that these interventions are not just effective, but also practical and well-received by patients (76, 118).
Finally, given the intricate relationship between diet, lifestyle, and ovarian health, a comprehensive approach is necessary. Future research should examine the influence of other lifestyle elements, such as physical activity and stress, on ovarian resilience, in connection with an unbalanced diet rich in fats (121). Moreover, the potential of personalized medicine strategies, considering individual variations in genetics, diet, and lifestyle, should be investigated (122). This approach acknowledges that each female is unique, and interventions must be customized to address their specific needs. In the light of this, the concept of nurturing ovarian resilience presents exciting new directions for understanding and enhancing female reproductive health. By further exploring these uncharted avenues, it is hoped that new strategies for promoting ovarian health and overall wellbeing can be discovered. These strategies will not only improve reproductive outcomes but also enhance the quality of life worldwide.
A comprehensive overview of the topics discussed in this section can be found in Figure 3.
Figure 3. Biological matrices-derived antioxidants as dietary components: their influence on ovarian health and modulation of the complex interplay between high-fat diet-induced oxidative stress and reproductive function. Adipose tissue derived from a high-fat diet regimen can precipitate the production of reactive oxygen species (ROS) and subsequent oxidative stress through multifaceted pathways. Notably, the activation of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase prompts ROS generation, fostering the upregulation of adipocytokines and cytokines, thus inciting inflammation and facilitating macrophage infiltration into adipose tissue. Furthermore, the heightened levels of ROS induce dysfunction within mitochondrial complex II, disrupting the electron transport chain and exacerbating ROS production. This intricate interplay underscores the pivotal role of oxidative stress in the pathophysiology of adipose tissue dysfunction amidst high-fat dietary habits. Connecting this phenomenon to fatty acid metabolism elucidates a complementary narrative, where electron transfer along the electron transport chain generates ROS as byproducts. Specifically, fatty acid metabolism within mitochondria serves as a prime site for ROS generation, with various complexes along the electron transport chain serving as key contributors. Consequently, the accrual of ROS can overwhelm endogenous antioxidant defenses, precipitating oxidative stress and consequent cellular damage. Such insights underscore the significance of biological matrices endowed with antioxidant properties, as they offer promising avenues for ameliorating the adverse effects of oxidative stress on physiological processes, including reproductive health. SOD, superoxide dismutase; GPx, glutathione peroxidase; GSSG, glutathione oxidase; GSH, glutathione reductase; ROS, reactive oxygen species; O2-•, superoxide; H2O2, hydrogen peroxide; OH•, Hydroxyl. Image created with Biorender.com.
4 Discussion and concluding insights: the impact of antioxidants derived from biological matrices on ovarian health and reproductive wellbeing in the context of HFD
Oxidative stress, characterized by an imbalance where the production of ROS exceeds the body's antioxidant defenses, can significantly influence various health conditions. A diet high in saturated fats can trigger an overproduction of ROS, leading to oxidative stress, which may negatively impact female reproductive health. Different in vivo research studies on preclinical models have highlighted how the use of biological matrices with antioxidant properties can contribute to improving conditions associated with an HFD. This improvement is especially evident in the activation of steroidogenesis and the synchronized growth of both the somatic and germinal compartments of the ovarian follicle, resulting in enhanced oocyte quality.
However, further efforts are needed to understand the complex relationship between the potential of these biological matrices in promoting female reproductive health and mitigating the harmful effects of an HFD. These efforts require not only enhancing lab studies to examine the early stages of ovarian follicle development but also gathering extensive data from animal studies before advancing to human trials. With these insights, the concept of bolstering ovarian resilience presents exciting opportunities to deepen our understanding and enhance female reproductive health. As these yet-to-be-explored paths are investigated, it is expected that innovative approaches will emerge, improving overall reproductive wellbeing and enriching the quality of life world-wide.
Data availability statement
All relevant data is contained within the article: The original contributions presented in the study are included in the article/Supplementary material, further inquiries can be directed to the corresponding author.
Author contributions
CD: Data curation, Formal analysis, Investigation, Methodology, Writing – original draft, Writing – review & editing. UB: Data curation, Formal analysis, Investigation, Methodology, Writing – original draft, Writing – review & editing. CC: Data curation, Formal analysis, Investigation, Writing – review & editing. AP: Data curation, Formal analysis, Investigation, Writing – review & editing. GC: Data curation, Formal analysis, Investigation, Writing – review & editing. NB: Writing – review & editing. VR: Writing – review & editing. VG: Writing – review & editing. FK: Writing – review & editing. MD: Writing – review & editing. BB: Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing.
Funding
The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. This research was funded by the European Union—Next Generation EU. Project Code: ECS00000041; Project CUP: C43C22000380007; Project Title: Innovation, digitalization, and sustainability for the diffused economy in Central Italy—VITALITY and by PRIN PNRR 2022—Prot. P20222H7J7—Title: Profiling redox, epigenetic and transgenerational effects induced by micro-nanoplastics: focus on female reprotoxicity and protective natural antioxidants in mammals.
Acknowledgments
The authors thank the European Union—Next Generation EU. Project Code: ECS00000041; Project CUP: C43C22000380007; Project Title: Innovation, digitalization, and sustainability for the diffused economy in Central Italy—VITALITY, the PRIN PNRR 2022—Prot. P20222H7J7—Title: Profiling redox, epigenetic and transgenerational effects induced by micro-nanoplastics: focus on female reprotoxicity and protective natural antioxidants in mammals and the European Union—Next Generation EU—DM 117 3.3—Dottorati innovativi che rispondono ai fabbisogni di innovazione delle imprese. CUP: C46E23000130004; Fellowship code: DM11733-10576. All individuals included in this section have consented to the acknowledgment.
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision.
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Supplementary material
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fnut.2024.1415455/full#supplementary-material
References
1. Vincent HK, Taylor AG. Biomarkers and potential mechanisms of obesity-induced oxidant stress in humans. Int J Obes. (2006) 30:400–18. doi: 10.1038/sj.ijo.0803177
2. Brewer CJ, Balen AH. The adverse effects of obesity on conception and implantation. Reproduction. (2010) 140:347–64. doi: 10.1530/REP-09-0568
3. Wilkes S, Murdoch A. Obesity and female fertility: a primary care perspective. J Fam Plan Reprod Health Care. (2009) 35:181–5. doi: 10.1783/147118909788707995
4. Gonnella F, Konstantinidou F, Di Berardino C, Capacchietti G, Peserico A, Russo V, et al. A systematic review of the effects of high-fat diet exposure on oocyte and follicular quality: a molecular point of view. Int J Mol Sci. (2022) 23:8890. doi: 10.3390/ijms23168890
5. Di Berardino C, Peserico A, Capacchietti G, Zappacosta A, Bernabò N, Russo V, et al. High-fat diet and female fertility across lifespan: a comparative lesson from mammal models. Nutrients. (2022) 14:4341. doi: 10.3390/nu14204341
6. Wei W, Qin F, Gao J, Chang J, Pan X, Jiang X, et al. The effect of maternal consumption of high-fat diet on ovarian development in offspring. Anim Reprod Sci. (2023) 255:107294. doi: 10.1016/j.anireprosci.2023.107294
7. Dunning KR, Russell DL, Robker RL. Lipids and oocyte developmental competence: the role of fatty acids and β-oxidation. Reproduction. (2014) 148:R15–27. doi: 10.1530/REP-13-0251
8. Skoracka K, Ratajczak AE, Rychter AM, Dobrowolska A, Krela-Kazmierczak I. Female fertility and the nutritional approach: the most essential aspects. Adv Nutr. (2021) 12:2372–86. doi: 10.1093/advances/nmab068
9. Kaltsas A, Zikopoulos A, Moustakli E, Zachariou A, Tsirka G, Tsiampali C, et al. The silent threat to women's fertility: uncovering the devastating effects of oxidative stress. Antioxidants. (2023) 12:1490. doi: 10.3390/antiox12081490
10. Shelling AN, Ahmed Nasef N. The role of lifestyle and dietary factors in the development of premature ovarian insufficiency. Antioxidants. (2023) 12:1601. doi: 10.3390/antiox12081601
11. Immediata V, Ronchetti C, Spadaro D, Cirillo F, Levi-setti PE. Oxidative stress and human ovarian response-from somatic ovarian cells to oocytes damage: a clinical comprehensive narrative review. Antioxidants. (2022) 11:1335. doi: 10.3390/antiox11071335
12. Horvath TL, Andrews ZB, Diano S. Fuel utilization by hypothalamic neurons: roles for ROS. Trends Endocrinol Metab. (2009) 20:78–87. doi: 10.1016/j.tem.2008.10.003
13. Aitken RJ, Bromfield EG, Gibb Z, Oxidative Stress and Reproductive Function. The impact of oxidative stress on reproduction: a focus on gametogenesis and fertilization. Reproduction. (2022) 164:F79–94. doi: 10.1530/REP-22-0126
14. Bełtowski J. Leptin and the regulation of endothelial function in physiological and pathological conditions. Clin Exp Pharmacol Physiol. (2012) 39:168–78. doi: 10.1111/j.1440-1681.2011.05623.x
15. Fernández-Sánchez A, Madrigal-Santillán E, Bautista M, Esquivel-Soto J, Morales-González Á, Esquivel-Chirino C, et al. Inflammation, oxidative stress, and obesity. Int J Mol Sci. (2011) 12:3117–32. doi: 10.3390/ijms12053117
16. Muñoz A, Costa M. Nutritionally mediated oxidative stress and inflammation. Oxid Med Cell Longev. (2013) 2013:610950. doi: 10.1155/2013/610950
17. Jungheim ES, Schoeller EL, Marquard KL, Louden ED, Schaffer JE, Moley KH. Diet-induced obesity model: abnormal oocytes and persistent growth abnormalities in the offspring. Endocrinology. (2010) 151:4039–46. doi: 10.1210/en.2010-0098
18. Moraes-Souza RQ, Vesentini G, Paula VG, Sinzato YK, Soares TS, Gelaleti RB, et al. Oxidative stress profile of mothers and their offspring after maternal consumption of high-fat diet in rodents: a systematic review and meta-analysis. Oxid Med Cell Longev. (2021) 2021:9073859. doi: 10.1155/2021/9073859
19. Hussain T, Murtaza G, Metwally E, Kalhoro DH, Kalhoro MS, Rahu BA, et al. The role of oxidative stress and antioxidant balance in pregnancy. Mediators Inflamm. (2021) 2021:9962860. doi: 10.1155/2021/9962860
20. Chatzakis C, Sotiriadis A, Tsakmaki E, Papagianni M, Paltoglou G, Dinas K, et al. The effect of dietary supplements on oxidative stress in pregnant women with gestational diabetes mellitus: a network meta-analysis. Nutrients. (2021) 13:2284. doi: 10.3390/nu13072284
21. Liang J, Gao Y, Feng Z, Zhang B, Na Z, Li D. Reactive oxygen species and ovarian diseases: antioxidant strategies. Redox Biol. (2023) 62:102659. doi: 10.1016/j.redox.2023.102659
22. Showell MG, Mackenzie-Proctor R, Jordan V, Hart RJ. Antioxidants for female subfertility. Cochrane Database Syst Rev. (2020) 8:CD007807. doi: 10.1002/14651858.CD007807.pub4
23. Pasquariello R, Verdile N, Brevini TAL, Gandolfi F, Boiti C, Zerani M, et al. The role of resveratrol in mammalian reproduction. Molecules. (2020) 25:4554. doi: 10.20944/preprints202009.0003.v1
24. Mushtaq F, Akhtar MF, Saleem A, Sharif A, Akhtar B, Askary A, et al. Berberis aristata DC extract counteracts the high fat diet-induced reproductive toxicity in female Wistar rats via modulating oxidative stress and resistance to leptin and insulin. Endocr Metab Immune Disord Drug Targets. (2022) 22:1390–402. doi: 10.2174/1871530322666220429125241
25. Chaudhari HS, Bhandari U, Khanna G. Preventive effect of embelin from embelia ribes on lipid metabolism and oxidative stress in high-fat diet-induced obesity in rats. Planta Med. (2012) 78:651–7. doi: 10.1055/s-0031-1298379
26. Sharifi-Rad M, Anil Kumar NV, Zucca P, Varoni EM, Dini L, Panzarini E, et al. Lifestyle, oxidative stress, and antioxidants: back and forth in the pathophysiology of chronic diseases. Front Physiol. (2020) 11:694. doi: 10.3389/fphys.2020.00694
27. Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, et al. Oxidative stress: harms and benefits for human health. Oxid Med Cell Longev. (2017) 2017:8416763. doi: 10.1155/2017/8416763
28. Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: impact on human health. Pharmacogn Rev. (2010) 4:118–26. doi: 10.4103/0973-7847.70902
29. Vašková J, Klepcová Z, Špaková I, Urdzík P, Štofilová J, Bertková I, et al. The importance of natural antioxidants in female reproduction. Antioxidants. (2023) 12:907. doi: 10.3390/antiox12040907
30. Page MJ, McKenzie JE, Bossuyt PM, Boutron I, Hoffmann TC, Mulrow CD, et al. The PRISMA 2020 statement: an updated guideline for reporting systematic reviews. BMJ. (2021) 372:n71. doi: 10.1136/bmj.n71
31. Snider AP, Wood JR. Obesity induces ovarian inflammation and reduces oocyte quality. Reproduction. (2019) 158:R79–90. doi: 10.1530/REP-18-0583
32. Shah JS, Sabouni R, Cayton Vaught KC, Owen CM, Albertini DF, Segars JH. Biomechanics and mechanical signaling in the ovary: a systematic review. J Assist Reprod Genet. (2018) 35:1135–48. doi: 10.1007/s10815-018-1180-y
33. Shea LD, Woodruff TK, Shikanov A. Bioengineering the ovarian follicle microenvironment. Annu Rev Biomed Eng. (2014) 16:29–52. doi: 10.1146/annurev-bioeng-071813-105131
34. Hsueh AJW, Kawamura K, Cheng Y, Fauser BCJM. Intraovarian control of early folliculogenesis. Endocr Rev. (2015) 36:1–24. doi: 10.1210/er.2014-1020
35. Ouni E, Vertommen D, Amorim CA. The human ovary and future of fertility assessment in the post-genome era. Int J Mol Sci. (2019) 20:4209. doi: 10.3390/ijms20174209
36. Gilchrist RB, Lane M, Thompson JG. Oocyte-secreted factors: regulators of cumulus cell function and oocyte quality. Hum Reprod Update. (2008) 14:159–77. doi: 10.1093/humupd/dmm040
37. Tatone C, Di Emidio G. Mitochondria biology in reproductive function. Antioxidants. (2022) 11:1978. doi: 10.3390/antiox11101978
38. Kinnear HM, Tomaszewski CE, Chang FL, Moravek MB, Xu M, Padmanabhan V, et al. The ovarian stroma as a new frontier. Reproduction. (2020) 160:R25–39. doi: 10.1530/REP-19-0501
39. Reeves G. Specific stroma in the cortex and medulla of the ovary. Cell types and vascular supply in relation to follicular apparatus and ovulation. Obstet Gynecol. (1971) 37:832–44.
40. Siu MKY, Cheng CY. The blood-follicle barrier (BFB) in disease and in ovarian function. Adv Exp Med Biol. (2012) 763:186–92. doi: 10.1007/978-1-4614-4711-5_9
41. Rolaki A, Drakakis P, Millingos S, Loutradis D, Makrigiannakis A. Novel trends in follicular development, atresia and corpus luteum regression: a role for apoptosis. Reprod Biomed Online. (2005) 11:93–103. doi: 10.1016/S1472-6483(10)61304-1
42. Vinatier D, Dufour P, Tordjeman-Rizzi N, Prolongeau JF, Depret-Moser S, Monnier JC. Immunological aspects of ovarian function: role of the cytokines. Eur J Obstet Gynecol. (1995) 63:155–68. doi: 10.1016/0301-2115(95)02227-9
43. Almeida GHDR, Iglesia RP, Rinaldi JDC, Murai MK, Calomeno CVAQ, Da Silva Junior LN, et al. Current trends on bioengineering approaches for ovarian microenvironment reconstruction. Tissue Eng Part B Rev. (2023) 29:260–98. doi: 10.1089/ten.teb.2022.0171
44. Henning NFC, Jakus AE, Laronda MM. Building organs using tissue-specific microenvironments: perspectives from a bioprosthetic ovary. Trends Biotechnol. (2021) 39:824–37. doi: 10.1016/j.tibtech.2021.01.008
45. Agarwal A, Aponte-Mellado A, Premkumar BJ, Shaman A, Gupta S. The effects of oxidative stress on female reproduction: a review. Reprod Biol Endocrinol. (2012) 10:49. doi: 10.1186/1477-7827-10-49
46. Ruder EH, Hartman TJ, Goldman MB. Impact of oxidative stress on female fertility. Curr Opin Obstet Gynecol. (2009) 21:219–22. doi: 10.1097/GCO.0b013e32832924ba
47. Lu J, Wang Z, Cao J, Chen Y, Dong Y. A novel and compact review on the role of oxidative stress in female reproduction. Reprod Biol Endocrinol. (2018) 16:80. doi: 10.1186/s12958-018-0391-5
49. Van Langendonckt A, Casanas-Roux F, Donnez J. Oxidative stress and peritoneal endometriosis. Fertil Steril. (2002) 77:861–70. doi: 10.1016/S0015-0282(02)02959-X
50. Agarwal A, Allamaneni SSR. Role of free radicals in female reproductive diseases and assisted reproduction. Reprod Biomed Online. (2004) 9:338–47. doi: 10.1016/S1472-6483(10)62151-7
51. Chan AC. Partners in defense, vitamin E and vitamin C. Can J Physiol Pharmacol. (1993) 71:725–31. doi: 10.1139/y93-109
52. Fontana R, Della Torre S. The deep correlation between energy metabolism and reproduction: a view on the effects of nutrition for women fertility. Nutrients. (2016) 8:87. doi: 10.3390/nu8020087
53. Jurczewska J, Szostak-Wegierek D. The influence of diet on ovulation disorders in women-a narrative review. Nutrients. (2022) 14:1556. doi: 10.3390/nu14081556
54. Novielli C, Anelli GM, Lisso F, Marzorati A, Parrilla B, Oneta M, et al. Effects of α-lipoic acid and myo-inositol supplementation on the oocyte environment of infertile obese women: a preliminary study. Reprod Biol. (2020) 20:541–6. doi: 10.1016/j.repbio.2020.10.002
55. El-Sayyad HIH, El-Shershaby EMF, El-Mansi AA, El-Ashry NE. Anti-hypercholesterolemic impacts of barley and date palm fruits on the ovary of Wistar albino rats and their offspring. Reprod Biol. (2018) 18:236–51. doi: 10.1016/j.repbio.2018.07.003
56. Jia Z, Feng Z, Wang L, Li H, Wang H, Xu D, et al. Resveratrol reverses the adverse effects of a diet-induced obese murine model on oocyte quality and zona pellucida softening. Food Funct. (2018) 9:2623–33. doi: 10.1039/C8FO00149A
57. Majd NE, Azizian H, Tabandeh M, Shahriari A. Effect of Abelmoschus esculentus powder on ovarian histology, expression of apoptotic genes and oxidative stress in diabetic rats fed with high fat diet. Iran J Pharm Res. (2019) 18:369–82. doi: 10.22037/IJPR.2019.2325
58. Nilsson MI, May L, Roik LJ, Fuda MR, Luo A, Hettinga BP, et al. Multi-ingredient supplement protects against obesity and infertility in western diet-fed mice. Nutrients. (2023) 15:611. doi: 10.3390/nu15030611
59. Han L, Wang H, Li L, Li X, Ge J, Reiter RJ, et al. Melatonin protects against maternal obesity-associated oxidative stress and meiotic defects in oocytes via the SIRT3-SOD2-dependent pathway. J Pineal Res. (2017) 63:12431. doi: 10.1111/jpi.12431
60. Zhuo Y, Cao M, Gong Y, Tang L, Jiang X, Li Y, et al. Gut microbial metabolism of dietary fibre protects against high energy feeding induced ovarian follicular atresia in a pig model. Br J Nutr. (2021) 125:38–49. doi: 10.1017/S0007114520002378
61. Basha EH, Eltokhy AKB, Eltantawy AF, Heabah NAE, Elshwaikh SL, El-Harty YM. Linking mitochondrial dynamics and fertility: promoting fertility by phoenixin through modulation of ovarian expression of GnRH receptor and mitochondrial dynamics proteins DRP-1 and Mfn-2. Pflugers Arch Eur J Physiol. (2022) 474:1107–19. doi: 10.1007/s00424-022-02739-y
62. Shams F, Aghajani-nasab M, Ramezanpour M, Fatideh RH, Mohammadghasemi F. Effect of apple vinegar on folliculogenesis and ovarian kisspeptin in a high-fat diet-induced nonalcoholic fatty liver disease in rat. BMC Endocr Disord. (2022) 22:1–13. doi: 10.1186/s12902-022-01205-1
63. Esencan E, Beroukhim G, Seifer DB. Age-related changes in folliculogenesis and potential modifiers to improve fertility outcomes - a narrative review. Reprod Biol Endocrinol. (2022) 20:156. doi: 10.1186/s12958-022-01033-x
64. Wang L, Tang J, Wang L, Tan F, Song H, Zhou J, et al. Oxidative stress in oocyte aging and female reproduction. J Cell Physiol. (2021) 236:7966–83. doi: 10.1002/jcp.30468
65. Ahmed AA, Fedail JS, Musa HH, Musa TH, Sifaldin AZ. Gum Arabic supplementation improved antioxidant status and alters expression of oxidative stress gene in ovary of mice fed high fat diet. Middle East Fertil Soc J. (2016) 21:101–8. doi: 10.1016/j.mefs.2015.10.001
66. Shoaib M, Saleem A, Zeb A, Khan MI, Akhtar MF. Chemical characterization and ameliorating effect of Centratherum anthelminticum extract against polycystic ovary syndrome in Wistar rats. Int J Endocrinol. (2023) 2023:4978562. doi: 10.1155/2023/4978562
67. Harphoush S, Wu G, Qiuli G, Zaitoun M, Ghanem M, Shi Y, et al. Thymoquinone ameliorates obesity-induced metabolic dysfunction, improves reproductive efficiency exhibiting a dose-organ relationship. Syst Biol Reprod Med. (2019) 65:367–82. doi: 10.1080/19396368.2019.1626933
68. Ding Y, Jiang Z, Xia B, Zhang L, Zhang C, Leng J. Mitochondria-targeted antioxidant therapy for an animal model of PCOS-IR. Int J Mol Med. (2019) 43:316–24. doi: 10.3892/ijmm.2018.3977
69. Huang Y, Zhang X. Luteolin alleviates polycystic ovary syndrome in rats by resolving insulin resistance and oxidative stress. Am J Physiol Endocrinol Metab. (2021) 320:E1085–92. doi: 10.1152/ajpendo.00034.2021
70. Wen X, Han Z, Liu SJ, Hao X, Zhang XJ, Wang XY, et al. Phycocyanin Improves reproductive ability in obese female mice by restoring ovary and oocyte quality. Front Cell Dev Biol. (2020) 8:595373. doi: 10.3389/fcell.2020.595373
71. Alshafei MM, Mohammed DM, Hanafi EM, Kassem SS, El-Messery TM, Korany RMS, et al. Red beet peel-loaded liposome's impact on metabolic syndrome rats and its use as fortifier in yoghurt powder. Egypt J Chem. (2023) 66:517−33.
72. Hanafi EM, Korany RMS, Tawfeek FM, Hozyen HF, El Natat WS, Ramadan MM, et al. Effect of frankincense (Boswellia Carterii) on animal reproductive performance. Egypt J Chem. (2023) 66:213–23.
73. Boots CE, Boudoures A, Zhang W, Drury A, Moley KH. Obesity-induced oocyte mitochondrial defects are partially prevented and rescued by supplementation with co-enzyme Q10 in a mouse model. Hum Reprod. (2016) 31:2090–7. doi: 10.1093/humrep/dew181
74. Tümentemur G, Altunkaynak BZ, Kaplan S. Is melatonin, leptin or their combination more effective on oxidative stress and folliculogenesis in the obese rats? J Obstet Gynaecol. (2020) 40:116–27. doi: 10.1080/01443615.2019.1657816
75. Tesarik J. Towards personalized antioxidant use in female infertility: need for more molecular and clinical studies. Biomedicines. (2021) 9:1933. doi: 10.3390/biomedicines9121933
76. Hohos NM, Skaznik-Wikiel ME. High-fat diet and female fertility. Endocrinology. (2017) 158:2407–19. doi: 10.1210/en.2017-00371
77. Hohos NM, Cho KJ, Swindle DC, Skaznik-Wikiel ME. High-fat diet exposure, regardless of induction of obesity, is associated with altered expression of genes critical to normal ovulatory function. Mol Cell Endocrinol. (2018) 470:199–207. doi: 10.1016/j.mce.2017.10.016
78. Dong L, Teh DBL, Kennedy BK, Huang Z. Unraveling female reproductive senescence to enhance healthy longevity. Cell Res. (2023) 33:11–29. doi: 10.1038/s41422-022-00718-7
79. Yamamoto A, Harris HR, Vitonis AF, Chavarro JE, Missmer SA. A prospective cohort study of meat and fish consumption and endometriosis risk. Am J Obstet Gynecol. (2018) 219:178.e1–10. doi: 10.1016/j.ajog.2018.05.034
80. Missmer SA, Chavarro JE, Malspeis S, Bertone-Johnson ER, Hornstein MD, Spiegelman D, et al. prospective study of dietary fat consumption and endometriosis risk. Hum Reprod. (2010) 25:1528–35. doi: 10.1093/humrep/deq044
81. Hansen SO, Knudsen UB. Endometriosis, dysmenorrhoea and diet. Eur J Obstet Gynecol Reprod Biol. (2013) 169:162–71. doi: 10.1016/j.ejogrb.2013.03.028
82. Deutch B. Menstrual pain in Danish women correlated with low n-3 polyunsaturated fatty acid intake. Eur J Clin Nutr. (1995) 49:508–16.
83. Amini L, Chekini R, Nateghi MR, Haghani H, Jamialahmadi T, Sathyapalan T, et al. The effect of combined vitamin C and vitamin E supplementation on oxidative stress markers in women with endometriosis: a randomized, triple-blind placebo-controlled clinical trial. Pain Res Manag. (2021) 2021:5529741. doi: 10.1155/2021/5529741
84. Liu Y, Yu Z, Zhao S, Cheng L, Man Y, Gao X, et al. Oxidative stress markers in the follicular fluid of patients with polycystic ovary syndrome correlate with a decrease in embryo quality. J Assist Reprod Genet. (2021) 38:471–7. doi: 10.1007/s10815-020-02014-y
85. Herman R, Sever MJ, JaneŽ A, DolŽan V, Herman R, Sever MJ, et al. Interplay between oxidative stress and chronic inflammation in PCOS: the role of genetic variability in PCOS risk and treatment responses. Polycystic Ovarian Syndr. (2019) 7. doi: 10.5772/intechopen.88698
86. Panti AA, Shehu CE, Saidu Y, Tunau KA, Nwobodo EI, Jimoh A, et al. Oxidative stress and outcome of antioxidant supplementation in patients with polycystic ovarian syndrome (PCOS). Int J Reprod Contraception Obstet Gynecol. (2018) 7:1667–72. doi: 10.18203/2320-1770.ijrcog20181892
87. Bahramrezaie M, Amidi F, Aleyasin A, Saremi AT, Aghahoseini M, Brenjian S, et al. Effects of resveratrol on VEGF & HIF1 genes expression in granulosa cells in the angiogenesis pathway and laboratory parameters of polycystic ovary syndrome: a triple-blind randomized clinical trial. J Assist Reprod Genet. (2019) 36:1701–12. doi: 10.1007/s10815-019-01461-6
88. Tan Z, Gong X, Wang CC, Zhang T, Huang J. Diminished ovarian reserve in endometriosis: insights from in vitro, in vivo, and human studies-a systematic review. Int J Mol Sci. (2023) 24:15967. doi: 10.3390/ijms242115967
89. Shen H, Wang L, Gao M, Wei L, Liu A, Wang B, et al. The follicular fluid metabolome in infertile individuals between polycystic ovary syndrome and diminished ovarian reserve. Arch Biochem Biophys. (2022) 732:109453. doi: 10.1016/j.abb.2022.109453
90. Helbig M, Vesper AS, Beyer I, Fehm T. Does nutrition affect endometriosis? Geburtshilfe Frauenheilkd. (2021) 81:191–9. doi: 10.1055/a-1207-0557
91. Parazzini F, Viganò P, Candiani M, Fedele L. Diet and endometriosis risk: a literature review. Reprod Biomed Online. (2013) 26:323–36. doi: 10.1016/j.rbmo.2012.12.011
92. Han Y, Wu H, Sun S, Zhao R, Deng Y, Zeng S, et al. Effect of high fat diet on disease development of polycystic ovary syndrome and lifestyle intervention strategies. Nutrients. (2023) 15:2230. doi: 10.3390/nu15092230
93. Glueck CJ, Goldenberg N. Characteristics of obesity in polycystic ovary syndrome: etiology, treatment, and genetics. Metabolism. (2019) 92:108–20. doi: 10.1016/j.metabol.2018.11.002
94. Glueck CJ, Dharashivkar S, Wang P, Zhu B, Gartside PS, Tracy T, et al. Obesity and extreme obesity, manifest by ages 20-24 years, continuing through 32-41 years in women, should alert physicians to the diagnostic likelihood of polycystic ovary syndrome as a reversible underlying endocrinopathy. Eur J Obstet Gynecol Reprod Biol. (2005) 122:206–12. doi: 10.1016/j.ejogrb.2005.03.010
95. Legro RS. Obesity and PCOS: implications for diagnosis and treatment. Semin Reprod Med. (2012) 30:496–506. doi: 10.1055/s-0032-1328878
96. Fatemi F, Mohammadzadeh A, Sadeghi MR, Akhondi MM, Mohammadmoradi S, Kamali K, et al. Role of vitamin E and D3 supplementation in intra-cytoplasmic sperm injection outcomes of women with polycystic ovarian syndrome: a double blinded randomized placebo-controlled trial. Clin Nutr ESPEN. (2017) 18:23–30. doi: 10.1016/j.clnesp.2017.01.002
97. Phoswa WN, Khaliq OP. The role of oxidative stress in hypertensive disorders of pregnancy (preeclampsia, gestational hypertension) and metabolic disorder of pregnancy (gestational diabetes mellitus). Oxid Med Cell Longev. (2021) 2021:5581570. doi: 10.1155/2021/5581570
98. Burton GJ, Jauniaux E. Oxidative stress. Best Pract Res Clin Obstet Gynaecol. (2011) 25:287–99. doi: 10.1016/j.bpobgyn.2010.10.016
99. Duhig K, Chappell LC, Shennan AH. Oxidative stress in pregnancy and reproduction. Obstet Med. (2016) 9:113–6. doi: 10.1177/1753495X16648495
100. Brown MA, Magee LA, Kenny LC, Karumanchi SA, McCarthy FP, Saito S, et al. The hypertensive disorders of pregnancy: ISSHP classification, diagnosis & management recommendations for international practice. Pregnancy Hypertens. (2018) 13:291–310. doi: 10.1016/j.preghy.2018.05.004
101. Coughlan MT, Vervaart PP, Permezel M, Georgiou HM, Rice GE. Altered placental oxidative stress status in gestational diabetes mellitus. Placenta. (2004) 25:78–84. doi: 10.1016/S0143-4004(03)00183-8
102. Kaaja R, Rönnemaa T. Gestational diabetes: pathogenesis and consequences to mother and offspring. Rev Diabet Stud. (2008) 5:194–202. doi: 10.1900/RDS.2008.5.194
103. Chuffa LG A, Lupi LA, Cucielo MS, Silveira HS, Reiter RJ, Seiva FRF. Melatonin promotes uterine and placental health: potential molecular mechanisms. Int J Mol Sci. (2019) 21:300. doi: 10.3390/ijms21010300
104. Langston-cox A, Marshall SA, Lu D, Palmer KR, Wallace EM. Melatonin for the management of preeclampsia: a review. Antioxidants. (2021) 10:1–17. doi: 10.3390/antiox10030376
105. Zeng K, Gao Y, Wan J, Tong M, Lee AC, Zhao M, et al. The reduction in circulating levels of melatonin may be associated with the development of preeclampsia. J Hum Hypertens. (2016) 30:666–71. doi: 10.1038/jhh.2016.37
106. Hobson SR, Gurusinghe S, Lim R, Alers NO, Miller SL, Kingdom JC, et al. Melatonin improves endothelial function in vitro and prolongs pregnancy in women with early-onset preeclampsia. J Pineal Res. (2018) 65:12508. doi: 10.1111/jpi.12508
107. McCance DR, Holmes VA, Maresh MJA, Patterson CC, Walker JD, Pearson DWM, et al. Vitamins C and E for prevention of pre-eclampsia in women with type 1 diabetes (DAPIT): a randomised placebo-controlled trial. Lancet. (2010) 376:259–66. doi: 10.1016/S0140-6736(10)60630-7
108. Kalpdev A, Saha SC, Dhawan V. Vitamin C and E supplementation does not reduce the risk of superimposed PE in pregnancy. Hypertens Pregnancy. (2011) 30:447–56. doi: 10.3109/10641955.2010.507840
109. Ding J, Kang Y, Fan Y, Chen Q. Efficacy of resveratrol to supplement oral nifedipine treatment in pregnancy-induced preeclampsia. Endocr Connect. (2017) 6:595–600. doi: 10.1530/EC-17-0130
110. Salehi B, Martorell M, Arbiser JL, Sureda A, Martins N, Maurya PK, et al. Antioxidants: positive or negative actors? Biomolecules. (2018) 8:124. doi: 10.3390/biom8040124
111. Alexescu T-G, Sitar-Tăut AV, Clemente-Suárez VJ, Peris-Ramos HC, Redondo-Flórez L, Beltrán-Velasco AI, et al. Personalizing nutrition strategies: bridging research and public health. J Pers Med. (2024) 14:305. doi: 10.3390/jpm14030305
112. Reichman ME, Judd JT, Taylor PR, Nair PP, Jones DY, Campbell WS. Effect of dietary fat on length of the follicular phase of the menstrual cycle in a controlled diet setting. J Clin Endocrinol Metab. (1992) 74:1171–5. doi: 10.1210/jcem.74.5.1569164
113. Yan S, Wang F, Shi Q. The effect of maternal high-fat-diet mediated oxidative stress on ovarian function in mice offspring. Exp Ther Med. (2020) 20:1–1. doi: 10.3892/etm.2020.9264
114. Hill P, Garbaczewski L, Haley N, Wynder EL. Diet and follicular development. Am J Clin Nutr. (1984) 39:771–7. doi: 10.1093/ajcn/39.5.771
115. Gülçin I. Antioxidant activity of food constituents: an overview. Arch Toxicol. (2012) 86:345–91. doi: 10.1007/s00204-011-0774-2
116. Rodríguez-Yoldi MJ. Anti-inflammatory and antioxidant properties of plant extracts. Antioxidants. (2021) 10:921. doi: 10.3390/antiox10060921
117. Wang Y, Wu Y, Wang Y, Xu H, Mei X, Yu D, et al. Antioxidant properties of probiotic bacteria. Nutrients. (2017) 9:5521. doi: 10.3390/nu9050521
118. Roy P, Tomassoni D, Traini E, Martinelli I, Micioni Di Bonaventura MV, Cifani C, et al. Natural antioxidant application on fat accumulation: preclinical evidence. Antioxidants. (2021) 10:858. doi: 10.3390/antiox10060858
119. Hussain T, Tan B, Yin Y, Blachier F, Tossou MCB, Rahu N. Oxidative stress and inflammation: what polyphenols can do for us? Oxid Med Cell Longev. (2016) 2016:7432797. doi: 10.1155/2016/7432797
120. Nani A, Murtaza B, Khan AS, Khan NA, Hichami A. Antioxidant and anti-inflammatory potential of polyphenols contained in mediterranean diet in obesity: molecular mechanisms. Molecules. (2021) 26:985. doi: 10.3390/molecules26040985
121. Cowan S, Lim S, Alycia C, Pirotta S, Thomson R, Gibson-Helm M, et al. Lifestyle management in polycystic ovary syndrome - beyond diet and physical activity. BMC Endocr Disord. (2023) 23:14. doi: 10.1186/s12902-022-01208-y
Keywords: high-fat diet, biological matrix, antioxidant, ovarian health, folliculogenesis, oocyte quality, embryo development
Citation: Di Berardino C, Barceviciute U, Camerano Spelta Rapini C, Peserico A, Capacchietti G, Bernabò N, Russo V, Gatta V, Konstantinidou F, Donato M and Barboni B (2024) High-fat diet-negative impact on female fertility: from mechanisms to protective actions of antioxidant matrices. Front. Nutr. 11:1415455. doi: 10.3389/fnut.2024.1415455
Received: 10 April 2024; Accepted: 27 May 2024;
Published: 10 June 2024.
Edited by:
Rosaria Meccariello, University of Naples Parthenope, ItalyCopyright © 2024 Di Berardino, Barceviciute, Camerano Spelta Rapini, Peserico, Capacchietti, Bernabò, Russo, Gatta, Konstantinidou, Donato and Barboni. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Chiara Di Berardino, cdiberardino@unite.it