Cong Wang
Xinyu Qiao1- 1Center of Experimental Instrument, School of Food Science and Technology, Dalian Polytechnic University, Dalian, China
- 2Inner Mongolia Mengniu Dairy Industry (Group) Co., Ltd., Hohhot, China
- 3College of Food Science, Northeast Agricultural University, Harbin, China
Dairy products have become more common in people's daily diets in recent years, and numerous useful components derived from milk are widely employed in the food industry. Milk fat globule membrane (MFGM) is a kind of film that encases milk fat globules, and has been shown to have a high nutritional value. In this work, the protein, lipid, carbohydrate, and other components of MFGM are discussed, and also common separation, preparation, and analysis technologies, physicochemical properties, and functional features of MFGM are reviewed, to provide some guidance for the development and utilization of MFGM.
Highlights
- The identification of primary components of milk fat globule membrane (MFGM) was compared.
- The separation and preparation technologies of components in MFGM were illustrated.
- The bioactivities and applications of MFGM in functional food industry were reviewed.
Introduction
Milk fat in milk is secreted by breast cells and exists in the form of fat globules with a diameter of 0.2–15 μM. The milk fat globule membrane (MFGM) is a thin layer that surrounds the fat globules. The content of MFGM in milk was different, and the reports about MFGM accounted for 2–6% of fat globules (1, 2). These contents were reported due to the difference in collection season, collection stage, preparation method, and determination method of raw milk.
The structure of MFGM is constructed with three layers, and the thickness of MFGM is 10–50 nm (3). The inner membrane is a monolayer composed of proteins and polar lipids from the endoplasmic reticulum. It is the membrane that is covered by triglycerides when they are accumulated in the endoplasmic reticulum of breast epithelial cells and released into the cytoplasm in the way of budding and growth; the outer membrane is a double-layer membrane, which is composed of proteins and polar lipids from the plasma membrane at the top of the epithelial cells of the mammary gland (4), which is the fat globule in the cytoplasm. When released by mammary epithelial cells in the manner of budding expansion after reaching a specific size, this membrane is covered. Furthermore, some cytoplasm may persist between the inner and outer membranes after lipoglobulin production.
The research heat was low from MFGM's initial report in 1936 until 1970. MFGM research has steadily increased since the 1970's. The current focus of MFGM research is on the following aspects: composition identification, separation and preparation methods, physical and chemical properties, and functional feature analysis. In recent years, the application of MFGM in the food industry has attracted more and more attention. In this article, the separation, preparation, composition, physicochemical properties, functional properties, and development prospects of MFGM are reviewed.
Isolation and Preparation
Milk fat globule membrane can be separated and prepared from fresh milk, cream, casein, and butter whey. The yield of MFGM in the separation process is influenced by three primary elements: biological factors, such as raw milk type; physical factors, such as temperature; and chemical factors, such as salt ions. The findings suggest that the heat treatment temperature has a significant effect on the integrity and stability of MFGM in milk. The denaturation of protein in MFGM and its binding with whey protein is induced by heating the milk above 60°C (5). The freezing and thawing will lead to the instability of fat globules, which will cause serious damage to MFGM. The stability of MFGM is also affected by fast air intake during separation. The composition, physical, and chemical characteristics of MFGM isolation are also affected by the separation technique, the kind of raw materials, and the preparation of raw materials. Therefore, to avoid the effects of the above elements on MFGM, it is required to research and improve the separation procedure.
There are many reports on the preparation of MFGM with fresh milk as raw material, mainly including degreasing, cream cleaning, MFGM release, collection, drying, and other procedures (6). First, the milk is separated into cream and skimmed milk (7). The cream is washed with water or salt solution of 3–5 times volume for 2–3 times (3), to remove casein and whey protein in cream (8). The washed cream is cooled to 10°C and centrifuged to promote the separation of the fat phase and the water phase (9). The buttermilk and butter particles are obtained by filtering with a filter cloth. After adding water and melting at 40–60°C, the upper layer of centrifugation is butter, and the lower layer is butter whey. The mixture of butter whey and casein is used to obtain MFGM suspension. MFGM suspension is acidified, salted out, or ultracentrifuged to remove the residual lipids, and then dried to obtain MFGM (10). There are also some studies on the preparation of MFGM with casein powder as raw material, which is mainly divided into the following steps: dissolution, washing, centrifugal separation, and drying of casein powder (11). As a whole, the main process of separating MFGM from milk was shown in Figure 1.
Figure 1. Isolation and separation of the components in Milk fat globule membrane (MFGM) from bovine milk.
In the preparation technologies of MFGM, in addition to physical separation methods, such as salt solution, a filtration device can also be used to remove whey protein and casein in milk (12). In the subsequent infiltration process, reverse osmosis water was added to keep the feed rate unchanged. The MFGM was separated from the reflux by acidification (13).
In the dairy industry, butter whey is usually selected as the main source of MFGM (4). In this process, whey protein and casein are mainly removed from raw materials (14). Since MFGM is similar to casein in size, casein must be removed before microfiltration. In industry, MFGM is usually prepared by a two-step method: first, rennet or casein is used, and then MFGM is obtained by microfiltration. It can be obtained by adding sodium citrate into the cheese milk to remove the casein by microfiltration and then centrifuging at high speed.
Composition of MFGM
The composition of MFGM is complex, mainly composed of polar lipids and membrane-specific proteins. Due to different separation, purification, and analysis techniques, the composition of MFGM in the relevant literature is also quite different (15). At the same time, factors, such as lactation stage, season, feed, and fat globule size, also affect the content and composition of MFGM (16). There are many reports on the molecular types of protein components, lipid components, and polysaccharide components in MFGM, but there are great differences among them. The qualitative and quantitative analysis methods of these components are summarized in Figure 2.
Figure 2. Identification and the quantitative analysis of the components in MFGM.
Proteins in MFGM
According to the current publications, the protein content of milk MFGM is about 25–30%. Due to the different separation and preparation conditions and analysis methods of MFGM, the highest protein content in the industrial MFGM powder is about 60%. At the same time, the number of MFGM proteins reported in different studies was also different. Lu et al., identified 169 kinds of MFGM proteins in milk by the filter-aided sample preparation method (FASP) and Nano LC-MS/MS. In addition, 312, 554, 175, and 143 MFGM proteins were identified from human milk, cow milk, goat milk, and yak milk (17). Le et al., used polyacrylamide gel electrophoresis (SDS-PAGE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to identify 225 proteins from milk-derived MFGM (2). Michael et al., identified 244 and 133 kinds of proteins in whey protein concentrate and casein concentrate by LC-MS/MS combined with the shotgun method (18).
Among the MFGM proteins, there are 8 kinds with the highest abundance, including MUC1 and MUC1 six glycoproteins, xanthine oxidoreductase (XDH/XO), mucin 15 (MUC15/PAS III), fatty acid transporter (CD36/PAS IV), lactophilic lipoprotein and lectin (pas6/7), and two non-glycosylated proteins: adipogenic differentiation-associated protein (ADPH) and fatty acid-binding protein (FABP).
Lipids in MFGM
The main lipids in MFGM are polar lipids and neutral lipids (15). The main polar lipids can be divided into five types: phosphatidylcholine (PC: 19.2–37.3%), phosphatidylethanolamine (PE: 19.8–42%), sphingomyelin (SM: 18–34.1%), phosphatidylinositol (PI: 0.6–13.6%), and phosphatidylserine (PS: 1.9–16%) (19). Phosphatidylcholine and sphingomyelin are mainly located in the outer layer of the cell membrane. Phosphatidylethanolamine is mainly located in the outer layer of the cell membrane, and phosphatidylinositol and phosphatidylserine are concentrated in the phospholipid bilayer. Neutral lipids include triglyceride, diglyceride, monoglyceride, cholesterol, and other esters, of which triglyceride is the main part (accounting for 62% of the total lipid) (10). The content of neutral grease in MFGM is variable (56–80%), which largely depends on the separation method used in MFGM separation (20).
Sugars in MFGM
The carbohydrate in MFGM is mainly combined with proteins or lipids and exists in the form of glycoprotein or glycolipid. Glycolipids can be divided into neutral glycolipids and acid glycolipids (gangliosides). Ceramide is sphingosine, which is linked to fatty acids by an amide bond. Neutral glycolipids are composed of one or more carbohydrate residues connected to ceramide in the lipid portion, including galactosylceramide, glucoceramide, and lacer. Acid glycolipids are composed of oligosaccharides linked by ceramide to at least one sialic acid and various other residues through glycosidic bonds. Glycoproteins include mucin, butyrophilin (BTN), fatty acid transporter (CD36), milk lectin PAS 6 (52 kDa), and PAS 7 (47 kDa) (21).
There are few reports about the content of polysaccharides in MFGM and the differences are large. He et al., determined the mass fraction of polysaccharides in yak MFGM by phenol sulfuric acid method, which was about 0.1 mg/g. Ji et al., determined the mass fraction of polysaccharides from five kinds of mammals, including cow milk (8.54 mg/g), camel milk (5.90 mg/g), buffalo milk (5.06 mg/g), yak MFGM (3.16 mg/g), and goat milk (2.96 mg/g). It can be seen that the mass fraction of polysaccharides in MFGM is related to many factors, such as mammalian species, separation, and analysis methods of MFGM.
Functional Properties Of MFGM
Emulsifying
Milk fat globule membrane and its components are amphiphilic, and they are considered to be excellent emulsifiers. The results indicated that the emulsion created with MFGM and MFGM protein had lower viscosity and smaller droplet size than the emulsion prepared with MFGM lipid. The results of heat treatment showed that the emulsion prepared by MFGM was stable at the temperature range of 35–85°C, whereas the emulsion made from MFGM protein became unstable at temperatures above 65°C (22).
The emulsifying performance of MFGM is related to its composition, which is closely related to the raw materials and separation methods of MFGM. It was found that the MFGM rich material separated from the reconstituted milk by microfiltration had a better emulsifying performance by comparing the particle size distribution, viscosity, and stability of different emulsions (23, 24). The discovery of emulsifying properties of MFGM is also helpful to the food industry. Due to the poor bioavailability and absorption of β-carotene in the aqueous phase, its application in the food industry has been greatly restricted. MFGM can be used as an emulsifier to form emulsion with β-carotene, which can improve the dispersion and poor stability of β-carotene in water, so as to improve its bioavailability (25).
Foaming
Due to the emulsifying properties of MFGM and MFGM protein, adding an appropriate amount of MFGM and MFGM protein in whipping cream will cover fat droplets and form an aggregated network structure, which will cover the air mixed in the whipping process and increase the foaming rate. Within a certain time range, there is a positive correlation between the stirring time and the foaming rate.
Water Holding
Because of its high water-holding capacity, MFGM may prevent moisture loss and migration in the bread core, which reduces the problem of bread aging and hardening. It can also be used as a bread improver, which can not only increase the nutritional value of bread, but also improve the quality of bread.
Bioactivities Of MFGM
There are various functional components in MFGM. Reinhardt et al. discovered that 23% of MFGM proteins are involved in membrane or protein transport, 11% in fat transport or metabolism, 7% in protein synthesis, binding, or folding, 9% in carrier protein, 4% in immune protein, and 2% in β- and x-casein; the remaining 21% in protein function is unknown (26). These functions are mainly derived from the protein, lipid, and carbohydrate components of the MFGM, as shown in Figure 3. From the current situation reported, the functional characteristics of MFGM are mainly as follows.
Figure 3. Bioactivities of the components in MFGM.
Immunomodulatory Activity
Many components in MFGM have been proved to have many nutritional values. BTN has a positive impact on human central nervous system diseases. BTN has sequence homology with antibodies detected in autistic children's serum. It can participate in the immune regulation of autism, positively regulate autoimmune encephalomyelitis, and affect the pathogenesis of autism. Some studies have also shown that compared with infants who eat skimmed milk powder, MFGM as complementary food can regulate the metabolic abnormalities of malnourished infants, thus improving metabolic regulation and enhancing immunity (27).
Anti-intestinal Inflammatory Activity
Infants given commercially available formula had a greater incidence of acute otitis media, gastrointestinal, and respiratory tract infections within 1 year of birth, compared with breastfed infants, perhaps because of the absence of MFGM. Studies have shown that supplementing formula milk powder with bovine MFGM can help to reduce the incidence of acute otitis media in infants and the utilization rate of antipyretic drugs and has an immunomodulatory effect on the humoral response of pneumococcal vaccine (28).
In recent years, the research on the protective effect of milk agglutinin on the gastrointestinal tract has become a hot spot. Milk agglutinin can specifically bind with various rotaviruses, inhibit its replication, reduce the apoptosis of intestinal epithelial cells, and prevent rotavirus infection leading to gastroenteritis. Mexican newborn research discovered a link between milk lectin levels in breast milk and infant rotavirus infection.
In addition, glycoprotein, butyric glycoside, lactonectin, and mucus of MFGM have antiviral effects in vitro, whereas oligosaccharides can inhibit the binding of a variety of bacteria (including pneumococci) to mucosa (29). Mucin has been proved to reduce the adhesion of Yersinia enterocolitica to the intestinal mucus.
Antitumor Activity
In the milk of cattle, goat, buffalo, yak, and camel, MFGM of goat and buffalo showed a better effect in inducing apoptosis and reducing the activity of HT-29 cells (30). Colon cancer, breast cancer, and other malignancies are all inhibited by the lipid and protein components of MFGM. Studies have found that the FABP in MFGM can inhibit the growth of breast cancer cells in vitro at very low concentrations. Nerve sphingomyelin can induce cancer cells to stop growth, differentiation, and apoptosis through ceramide, sphingosine, and metabolites of colon cancer cells, which can effectively inhibit early and late colon cancer. In human breast cancer cells, the expression of milk agglutinin will be reduced the survival rate of cancer cells. Therefore, MFGM can be used as a nutritional supplement to inhibit the growth of cancer cells (31).
Promoting Cell Growth and Differentiation
Milk agglutinin can support the growth of epithelial cells. The purified MFGM protein can effectively promote cell growth and inhibit the apoptosis of C2C12 cells by upregulating the expression of Akt and mTOR protein kinase. MFGM has the potential to aid in illness prevention and also newborn growth and development (32). Studies have shown that adding MFGM to the diet of infants aged 6–11 months can prevent diarrhea, and adding MFGM to the diet of preschool children can improve the fever of children's language function (33). Feeding SM supplemented milk to preterm newborns with birth weights <1,500 g can compensate for neural development deficits caused by inadequate breast milk, resulting in a favorable influence on the infant's neural development (34). The combination of bovine MFGM and lactoferrin in the diet can not only better approach the bioactive components of breast milk, but also contribute to the cognitive, gastrointestinal, and respiratory health of infants (35).
Other Activities of MFGM
The MFGM differential proteins of yak and cow were shown to be involved in glycolipid metabolism, immunological regulation, antioxidant activity, anticancer, neuromodulation, antibacteria, and viral adhesion in marker-free proteomics. Both differential protein and protein-related genes predicted that yak and bovine MFGM protein had the biological function of regulating glucose and lipid metabolism. In vitro experiments confirmed that yak and bovine MFGM protein could reduce lipid accumulation and increase glucose uptake in HepG2 cells. The MFGM protein of yak and cattle has the higher lipid-lowering ability and increasing glucose uptake ability, which are respectively related to different MFGM proteins related to glucose and lipid metabolism in yak and cattle (36).
The elderly's feeling of balance, strength, and sensitivity can be improved by combining MFGM consumption with adequate exercise (37). Furthermore, the inclusion of MFGM may aid in the treatment of dyskinesia syndrome. Sphingolipids in MFGM can not only treat and improve cardiovascular diseases, but also participate in the inflammatory process of atherosclerosis and insulin resistance. Therefore, it can be inferred that dietary sphingolipids have great potential for the treatment of various aspects of metabolic syndromes, such as dyslipidemia, insulin resistance, and cardiovascular diseases.
Prospective
With the deep understanding of the composition and properties of MFGM, the nutritional function, growth and development of different populations, disease prevention, and other functional characteristics of MFGM have been gradually discovered, but the nutritional and functional characteristics of specific components of MFGM still need to be studied further. At present, infant formula with MFGM concentrate has been launched in the market. Special medical purpose formula food, special food, and functional food containing MFGM still have a sizable market. Furthermore, the research heat of the MFGM is increasing, and the fine separation and preparation technology is improving.
Author Contributions
CW, ZG, and LJ contributed to conception and design of the study. XQ organized the figures. ZM performed the statistical analysis. CW wrote the first draft of the manuscript. ZG and XQ wrote sections of the manuscript. All authors contributed to manuscript revision, read, and approved the submitted version.
Funding
This study was financially supported by the Basic Research Program of Liaoning Education Department (2017J080) and the National Natural Science Foundation of China (31371805).
Conflict of Interest
CW is a full-time faculty in the Dalian Polytechnic University and she directed a research work funded by the Inner Mongolia Mengniu Dairy Industry (Group) Co., Ltd., in which ZG and ZM engaged.
The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher's Note
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Supplementary Material
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fnut.2021.807284/full#supplementary-material
References
1. Smoczyński M, Staniewski B, Kiełczewska K. Composition and structure of the bovine milk fat globule membrane—some nutritional and technological implications. Food Rev Int. (2012) 28:188–202. doi: 10.1080/87559129.2011.595024
2. Le TT, Van Camp J, Dewettinck K. Milk fat globule membrane material: Isolation techniques, health-beneficial properties, and potential applications. Stud Nat Prod Chem. (2014) 7:347–82. doi: 10.1016/B978-0-444-63294-4.00012-7
3. Le TT, Van Camp J, Rombaut R, Van Leeckwyck F, Dewettinck K. Effect of washing conditions on the recovery of milk fat globule membrane proteins during the isolation of milk fat globule membrane from milk. J Dairy Sci. (2009) 92:3592–603. doi: 10.3168/jds.2008-2009
4. Guerin J, Burgain J, Gomand F, Scher J, Gaiani C. Milk fat globule membrane glycoproteins: valuable ingredients for lactic acid bacteria encapsulation? Crit Rev Food Sci Nutr. (2019) 59:639–51. doi: 10.1080/10408398.2017.1386158
5. He S, Li H, Ma Y. Composition and physiological characteristics of milk fat globule membrane. China Dairy Industry. (2009) 37:38–57. doi: 10.3389/fped.2018.00313
6. Dewettinck K, Rombaut R, Thienpont N, Le TT, Messens K, Van Camp J. Nutritional and technological aspects of milk fat globule membrane material. Int Dairy J. (2008) 18:436–57. doi: 10.1016/j.idairyj.2007.10.014
7. Li H, Xu W, Ma Y, Zhou S. Separation and purification of the bovine milk fat globule membrane protein and its effect on improvement of C2C12 mouse skeletal muscle cell proliferation. N J Chem. (2017) 41:6530–9. doi: 10.1039/C7NJ00560A
8. Jukkola A, Partanen R, Xiang W, Heino A, Rojas OJ. Food emulsifiers based on milk fat globule membranes and their interactions with calcium and casein phosphoproteins. Food Hydrocoll. (2019) 94:30–7. doi: 10.1016/j.foodhyd.2019.03.005
9. Qu X, Hu H, Wang Y, Cao C, Li H, Liu X, et al. Proteomics analysis of milk fat globule membrane enriched materials derived from by-products during different stages of milk-fat processing. LWT. (2019) 116:108531. doi: 10.1016/j.lwt.2019.108531
10. Kanno C, Kim D-H. A simple procedure for the preparation of bovine milk fat globule membrane and a comparison of its composition, enzymatic activities, and electrophoretic properties with those prepared by other methods. Agric Biol Chem. (1990) 54:2845–54. doi: 10.1080/00021369.1990.10870405
11. Corredig M, Dalgleish DG. Isolates from industrial buttermilk: emulsifying properties of materials derived from the milk fat globule membrane. J Agric Food Chem. (1997) 45:4595–600. doi: 10.1021/jf970531f
12. Hansen SF, Hogan SA, Tobin J, Rasmussen JT, Larsen LB, Wiking L. Microfiltration of raw milk for production of high-purity milk fat globule membrane material. J Food Eng. (2020) 276:109887. doi: 10.1016/j.jfoodeng.2019.109887
13. Hansen SF, Petrat-Melin B, Rasmussen JT, Larsen LB, Ostenfeld MS, Wiking L. Placing pasteurisation before or after microfiltration impacts the protein composition of milk fat globule membrane material. Int Dairy J. (2018) 81:35–41. doi: 10.1016/j.idairyj.2017.12.015
14. Holzmüller W, Kulozik U. Isolation of milk fat globule membrane (MFGM) material by coagulation and diafiltration of buttermilk. Int Dairy J. (2016) 63:88–91. doi: 10.1016/j.idairyj.2016.08.002
15. El-Loly MM. Composition, properties and nutritional aspects of milk fat globule membrane-a review. Pol J Food Nutr Sci. (2011) 61:11. doi: 10.2478/v10222-011-0001-0
16. Ahn Y-J, Ganesan P, Kwak H-S. Composition, structure, and bioactive components in milk fat globule membrane. Food Sci Anim Resourc. (2011) 31:1–8. doi: 10.5851/kosfa.2011.31.1.001
17. Cebo C, Caillat H, Bouvier F, Martin P. Major proteins of the goat milk fat globule membrane. J Dairy Sci. (2010) 93:868–76. doi: 10.3168/jds.2009-2638
18. Affolter M, Grass L, Vanrobaeys F, Casado B, Kussmann M. Qualitative and quantitative profiling of the bovine milk fat globule membrane proteome. J Proteomics. (2010) 73:1079–88. doi: 10.1016/j.jprot.2009.11.008
19. Lopez C. Milk fat globules enveloped by their biological membrane: unique colloidal assemblies with a specific composition and structure. Curr Opin Colloid Interface Sci. (2011) 16:391–404. doi: 10.1016/j.cocis.2011.05.007
20. Truong T, Lopez C, Bhandari B, Prakash S. Dairy Fat Products and Functionality. Berlin: Springer (2020). doi: 10.1007/978-3-030-41661-4
21. Gupta VK, Tuohy MG, O'donovan A, Lohani M. Biotechnology of Bioactive Compounds: Sources and Applications. Hoboken, NJ: John Wiley & Sons (2015). doi: 10.1002/9781118733103
22. He S, Tang H, Yi H, Xu W, Ma Y, Wang R. Properties of emulsions from milk fat globule membrane and its components. Int J Food Proper. (2017) 20:1342–53. doi: 10.1080/10942912.2017.1343348
23. Phan TTQ, Asaduzzaman M, Le TT, Fredrick E, Van Der Meeren P, Dewettinck K. Composition and emulsifying properties of a milk fat globule membrane enriched material. Int Dairy J. (2013) 29:99–106. doi: 10.1016/j.idairyj.2012.10.014
24. Phan TTQ, Le TT, Van Der Meeren P, Dewettinck K. Comparison of emulsifying properties of milk fat globule membrane materials isolated from different dairy by-products. J Dairy Sci. (2014) 97:4799–810. doi: 10.3168/jds.2014-8030
25. He S, Ye A. Formation and gastrointestinal digestion of β-carotene emulsion stabilized by milk fat globule membrane. J Food Process Eng. (2019) 42:e13301. doi: 10.1111/jfpe.13301
26. Reinhardt TA, Lippolis JD. Bovine milk fat globule membrane proteome. J Dairy Res. (2006) 73:406–16. doi: 10.1017/S0022029906001889
27. Zhang X, Wu Y, Ye H, Feng C, Han D, Tao S, et al. Dietary milk fat globule membrane supplementation during late gestation increased the growth of neonatal piglets by improving their plasma parameters, intestinal barriers, and fecal microbiota. RSC Adv. (2020) 10:16987–98. doi: 10.1039/D0RA02618B
28. Timby N, Hernell O, Vaarala O, Melin M, Lönnerdal B, Domellöf M. Infections in infants fed formula supplemented with bovine milk fat globule membranes. J Pediatr Gastroenterol Nutr. (2015) 60:384–9. doi: 10.1097/MPG.0000000000000624
29. Da Silva RC, Colleran HL, Ibrahim SA. Milk fat globule membrane in infant nutrition: a dairy industry perspective. J Dairy Res. (2021) 88:105–16. doi: 10.1017/S0022029921000224
30. Ji X, Xu W, Cui J, Ma Y, Zhou S. Goat and buffalo milk fat globule membranes exhibit better effects at inducing apoptosis and reduction the viability of HT-29 cells. Sci Rep. (2019) 9:1–14. doi: 10.1038/s41598-019-39546-y
31. Motouri M, Matsuyama H, Yamamura J-I, Tanaka M, Aoe S, Iwanaga T, et al. Milk sphingomyelin accelerates enzymatic and morphological maturation of the intestine in artificially reared rats. J Pediatr Gastroenterol Nutr. (2003) 36:241–7. doi: 10.1097/00005176-200302000-00016
32. Hernell O, Lonnerdal B, Timby N. Milk fat globule membranes: effects on microbiome metabolome and infections in infants and children. Nestle Nutr Inst Workshop Ser. (2020) 94:133–40. doi: 10.1159/000505066
33. Zavaleta N, Kvistgaard AS, Graverholt G, Respicio G, Guija H, Valencia N, et al. Efficacy of an MFGM-enriched complementary food in diarrhea, anemia, and micronutrient status in infants. J Pediatr Gastroenterol Nutr. (2011) 53:561–8. doi: 10.1097/MPG.0b013e318225cdaf
34. Tanaka K, Hosozawa M, Kudo N, Yoshikawa N, Hisata K, Shoji H, et al. The pilot study: sphingomyelin-fortified milk has a positive association with the neurobehavioural development of very low birth weight infants during infancy, randomized control trial. Brain Dev. (2013) 35:45–52. doi: 10.1016/j.braindev.2012.03.004
35. Li F, Wu SS, Berseth CL, Harris CL, Richards JD, Wampler JL, et al. Improved neurodevelopmental outcomes associated with bovine milk fat globule membrane and lactoferrin in infant formula: a randomized, controlled trial. J Pediatr. (2019) 215:24–31.e28. doi: 10.1016/j.jpeds.2019.08.030
36. Zhao L, Du M, Gao J, Zhan B, Mao X. Label-free quantitative proteomic analysis of milk fat globule membrane proteins of yak and cow and identification of proteins associated with glucose and lipid metabolism. Food Chem. (2019) 275:59–68. doi: 10.1016/j.foodchem.2018.09.044
37. Yoshinaka Y, Soga S, Ota N, Yokoyama K, Yamada Y, Kimura M. Light rhythmic exercise with dietary milk fat globule membrane improves physical fitness in an elderly Japanese population: a double-blind randomized placebo-controlled trial. Biosci Biotechnol Biochem. (2018) 82:677–82. doi: 10.1080/09168451.2017.1412248
Keywords: dairy industry, components, preparation, functionality, prosperity
Citation: Wang C, Qiao X, Gao Z, Jiang L and Mu Z (2022) Advancement on Milk Fat Globule Membrane: Separation, Identification, and Functional Properties. Front. Nutr. 8:807284. doi: 10.3389/fnut.2021.807284
Received: 02 November 2021; Accepted: 06 December 2021;
Published: 28 January 2022.
Edited by:
Fuguo Liu, Northwest A&F University, ChinaReviewed by:
Xianghe Meng, Zhejiang University of Technology, ChinaJinxuan Cao, Ningbo University, China
Xueying Mao, China Agricultural University, China
Copyright © 2022 Wang, Qiao, Gao, Jiang and Mu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Cong Wang, yuanque@163.com; Zhishen Mu, muzhishen@163.com