Effect of Extrusion or Fermentation on Physicochemical and Digestive Properties of Barley Powder

In this work, the effect of extrusion and fermentation on the physicochemical and digestive properties of barley powder was studied. The results showed that the contents of phenolics, β-glucan, protein, and lipid decreased after extrusion. The contents of nutrients (except lipid) increased after fermentation. Both extrusion and fermentation of barley can lead to the darkening of the color and effectively optimize the palatability by reducing the viscosity. In vitro digestion of starch showed that the content of as rapidly digestible starch increased after extrusion and fermentation. The contents of ferulic acid, 2-hydroxybenzoic acid, and caffeic acid decreased after extrusion, while the contents of chlorogenic acid, p-coumaric acid, and ferulic acid increased after fermentation. Basically, the content of all the phenolic showed an increasing trend after digestion. The antioxidant activity decreased after extrusion and increased after fermentation. Therefore, the nutritional composition and properties of barley powder were changed under the two processing methods.

Introduction

In recent years, metabolic syndrome, represented by obesity, hyperglycemia, hyperlipidemia, and diabetes mellitus, has become one of the most important non-communicable chronic diseases affecting human health. Barley is a new type of health food, whose overall nutritional index meets the requirements of high plant protein, high vitamin, high cellulose, low fat, and low sugar proposed by modern nutrition science. In addition, as a grain with a low glycemic index, barley can also be used to prevent chronic diseases such as diabetes and obesity (1).

Extrusion can greatly improve the digestibility of nutrients. Due to the short duration of the processing of food raw materials, the harmful effects of browning and nutritional damage on the nutrients of the raw materials are reduced to a large extent. Extrusion is a common processing method in the food industry, and it has the advantages of high production efficiency, simple process, low energy consumption, and low cost.

Fermentation is an effective method to improve the quality of barley. Lactobacillus plantarum is a common probiotic in food and it can regulate human cholesterol, blood pressure, and intestinal microorganism. Fermentation of barley using Lactobacillus plantarum can improve the nutritional value of barley and also can reduce the content of phytic acid. Therefore, the combination of Lactobacillus plantarum fermentation with barley is beneficial to improve its functional active components and enhance its physiological and nutritional functions (2).

This paper mainly studied the effects of extrusion and fermentation treatments on the nutrient composition, rheological, and digestive properties of barley powder, and analyzed the advantages and disadvantages of these changes.

Materials and Methods

Materials and Reagents

Yangzhou's No. 3 Barley Powder (BP) was purchased from Yancheng Shuangzeng Agrochemical Technology Co. LTD (Yancheng, China). Extruded Barley Powder (EBP, 40 Hz of screw rotation, 30 Hz of feeding speed, 140°C of sleeve temperature, and 30% of raw water content) and fermented barley powder (FBP, fermentation with Lactobacillus plantarum dy-1) were prepared in our laboratory.

α-amylase, glucosidase, pepsin, trypsin, and bile salt were purchased from Sigma Company (St. Louis, MO, United States) as biological reagents. Gallic acid, catechin, epicatechin, chlorogenic acid, ferulic acid, caffeic acid, 2-hydroxybenzoic acid, p-coumaric acid, vanillin acid, and 3, 4-dihydroxybenzoic acid were purchased from Tixi Ai (Shanghai) Chemical Industrial Development Co, Ltd.

Determination of Nutritional Components

Determination of Total Phenolics

The total phenolic content was determined by the method described by Rani et al. (3) with some modifications. Briefly, 1 ml of sample extract was diluted with 5 ml water and 3 ml of Na₂CO₃ (7.5%) was added. Subsequently, the test tube was kept for 90 min incubation in the 45°C water bath and absorbance was measured at 750 nm. A Gallic acid standard curve was made to determine the phenolic content.

Determination of β-Glucan

β-Glucan content was determined by the method described by 4 with some modifications. Briefly, 0.5 ml of sample extract was diluted with 1.5 ml water and 4 ml of Congo red solution was added. Subsequently, the test tube was kept for 10 min incubation in the 20°C water bath and absorbance was measured at 550 nm. A β-glucan standard curve was made to determine the β-glucan content.

Determination of Protein Content

The total nitrogen content was estimated by an azotometer (Milan, Italy). The protein content was calculated with the conversion factor of 6.25.

Determination of Fat Content

The fat content was determined by following the AOAC (2006) standard method number 2003.05.

Determination of Free Amino Acid Content

The content of free amino acids was determined by the amino acid analyzer according to the method of Hu et al. (5). The results were expressed as the percentage of each amino acid in the total amino acids with the exception of tryptophan.

Dissolving Characteristics and Rheological Characteristics

Determination of the Color

The colors of barley samples were determined using the HunterLab UltraScan Pro according to the method described by Morris et al. (6) with some modifications. The L^* value represents the lightness, 0–100 represents dark to light. The a^* value represents the degree of the red-green and the b^* value represents the degree of the yellow-blue.

Determination of WSI and WAI

The water solubility index (WSI) and water absorption index (WAI) were measured by the method of Haoqiang Wang et al. (7), with some modifications. A total of 2.5 g of samples were dispersed in 30 ml distilled water and shaken in the water bath at 30°C for 30 min, then centrifuged at 7000 × g for 15 min. The weight of the sediment was taken as WAI and was expressed as the unit g/g. The WSI is the weight of dry solids in the supernatant, which is expressed as a percentage of the original weight of the sample.

Determination of Pasting Properties

Pasting properties were determined by the method described by H. Wang et al. (8) with some modifications. Briefly, the pasting properties of samples were measured by a rapid visco-analyzer (RVA) combined with the Thermocline for Windows (TCW₃) software. The basic weight of the sample was 3 g (on a raw basis) and was automatically corrected to a moisture basis of 14%. Approximately, 3.0 g of samples and 25 ml of distilled water were directly added into an RVA canister. Then, the slurry was homogenized manually by the plastic paddle to avert lumps formation prior to the RVA run. The canister with the sample and paddle was inserted into the RVA Instrument and the analysis was started. The samples were heated from 50 to 95°C with a heating rate of 12°C/min, maintained at 95°C for 3.5 min, and then cooled to 50°C at the same rate and maintained at 50°C for 5 min. The following characteristics were then evaluated: peak viscosity, trough viscosity, final viscosity, breakdown, setback, peak time, and pasting temperature.

Static Viscoelasticity Analysis

The efficacy of barley in lowering blood lipid and cholesterol is closely related to its viscosity. The rheological property of the sample can be further analyzed by measuring the static viscoelasticity. Viscoelastic measurements were performed using a TA rheometer equipped with parallel plate geometry (40 mm). Firstly, 0.6 g sample was dissolved in 10 ml distilled water and mixed to prepare the solution with 6% concentration. After the fixture was installed and the calibration was carried out, about 1.5 ml sample solution was taken and dropped in the center of the plate. A frequency sweep test for starch gels was performed at 25°C from 0.01 to 1,000 s⁻¹ within the linear viscoelastic range.

In vitro Starch Digestion

The digestibility of starch was determined according to the procedure, as follows. First, 0.2 M sodium acetate buffer (pH 6.0 containing 200 mM calcium chloride, 0.49 mM magnesium chloride, and 0.02% sodium azide) was prepared and stored in a refrigerator at 4°C.

The in vitro starch digestion test: Each sample (50 mg) was suspended in a 50 ml centrifuge tube containing 2 ml deionized water, and then mixed well. Then, 8.0 ml of enzyme solution (37°C) was added to the tube, followed by incubation in a water bath at 37°C with shaking, at a stirring speed of 300 rpm. After the time periods of 0, 10, 20, 60, 120, and 180 min, the tube was shaken and 0.1 ml aliquot was quickly taken and transferred to a 1.5 ml centrifuge tube containing 0.9 ml absolute ethanol to deactivate the enzymes. The tube was then returned for further time course digestion.

Starch digestibility was calculated using the GOPOD method by determining the glucose content. Based on the hydrolysis rate, starch was defined as rapidly digestible starch (RDS), slowly digestible starch (SDS), and resistant starch (RS).

In vitro Digestion of Phenolic Compounds

Extraction of Phenolic Compounds

The extraction of polyphenolic compounds was performed according to the protocol of Horax et al. (9) with modifications. A total of 10 ml of a methanol solution was added to 1 g of each sample and stirred for 2 h at 200 rpm, then treated for 15 min in an ultrasonic bath and finally centrifuged for 10 min at 6,000 rpm. The pellet obtained was subjected to a second extraction and resuspended in 10 ml of methanol solution and the second extraction was performed as described before.

In vitro Digestion

A static model that simulated gastrointestinal digestion was developed, following the method described by Minekus et al. (10). The detailed composition of simulated salivary fluid (SSF), simulated gastric fluid (SGF), and simulated intestinal fluid (SIF) is given in Table 1, and they were prepared from stock solutions fresh daily and kept at 37°C before use.

TABLE 1

Table 1. Preparation of stock solution of simulated digestion fluids.

In vitro Simulation of Saliva Digestion (SD)

A total of 4 ml SSF was added to a 5.0 g barley powder sample and mixed well. Then, 15 mg α-amylase, 25 μL of CaCl₂ solution, and 975 μL water was added and shaken at 37°C for 2 min away from light.

In vitro Simulation of Gastric Digestion (GD)

A total of 160 mg pepsin (250 U/mg) was added to 8.0 ml SGF, and it is added to saliva digestive products. Then, 5 μL CaCl₂ solution and 1990 μL water were added. Thew pH was adjusted to 3 with HCl, mixed well, and shaken away from light for 2 h.

In vitro Simulation of Intestinal Digestion (ID)

About 144 mg bile salt was added to 16.0 ml SIF and stirred at 37°C for 20–30 min. Then, 535 mg trypsinase and 40 μL CaCl₂ solution were added to the gastric digestible products. The pH was adjusted to 7 with NaOH solution. After simulation, the samples were frozen at −20°C, lyophilized, and submitted to extraction as described above (Section Extraction of Phenolic Compounds).

Determination of Phenolic Composition

The individual phenolic compounds in the above extracts were quantified using a Shimazu LC-20AD HPLC system. A ZORBAX SB-Aq column (5 m, 4.6 mm × 250 mm ID, Agilent) was used at a column temperature of 30°C. The mobile phase consisted of a 0.1% aqueous solution of formic acid (solution A) and acetonitrile (solution B) with the following gradient program: 0–10 min, solution B 3–3%; 10–20 min, solution B 3–80%; 20–30 min, solution B 80–3%; 30–35 min, solution B 3–3%. Other chromatographic conditions included a constant flow rate of 0.8 ml/min, an injection volume of 20 μL, a run time of 35 min, and a detection wavelength of 254 and 320 nm. Prior to analysis, all samples were filtered through a 0.25–l m membrane filter. The identification of each peak was based on the retention time and the chromatography of authentic standards. The concentrations of each compound were calculated according to a standard curve, and the results were expressed as μg/g DW of barley powder.

Determination of Antioxidant Activity

Determination of DPPH Radical Scavenging Activity

The method for determination of DPPH radical scavenging ability is based on measurement of the loss of DPPH radical color at 517 nm after reaction with sample extract and was performed as described by Brand–Williams et al. (11). Sample extracts (2 ml) were mixed with 2 ml of DPPH radical solution, incubating for 30 min in the dark and measuring absorbance at 517 nm. Sample extracts and anhydrous ethanol were used as a control group. Anhydrous ethanol and DPPH radical solution were used as a blank group. Results were expressed as the percentage of free radical scavenging.

Determination of ABTS Radical Cation Scavenging Activity

2, 2′ -azino-BIS (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation scavenging capacity was measured using the modified method described by Sun et al. (12). Diluted the absorbance of ABTS radical cation solution to 0.70 ± 0.02 at 734 nm. Extracts (0.1 ml) were mixed with 2 ml ABTS radical cation, incubating 2–6 min, and measuring the absorbance at 734 nm. Sample extracts and anhydrous ethanol were used as a control group. Anhydrous ethanol and ABTS radical cation were used as the blank group. Results were expressed as the percentage of free radical scavenging.

Determination of Ferric Reducing Antioxidant Power-FRAP Method

Ferric Reducing Antioxidant Power (FRAP) assay was performed according to a modified method reported by Benzie and Strain (13). Fresh FRAP working reagent was prepared by mixing 25 ml 300 mM acetate buffer, 2.5 ml TPTZ solution, and 2.5 ml of 20 mM FeCl₃•6H₂O solution. FeSO₄ solution was used as the standard solution. A total of 180 μL FRAP solution was mixed with the standard solution in different concentrations and sample extracts, respectively. After incubation at 37°C for 3–5 min, the absorbance was detected at 593 nm.

Statistical Analysis

Each experiment was carried out in triplicate. Experimental design and data analysis were performed with IBM SPSS statistical software. Data were analyzed by ANOVA and expressed as mean ± SD. P < 0.05 was considered to be statistically significant.

Results and Discussion

Nutritional Components Analysis

The Content of Free Phenol, β-Glucan, Protein, and Fat

To evaluate the effects of extrusion and fermentation of barley powder, changes in main nutrients and amino acid contents were determined, as shown in Table 2. Compared with BP, the content of free phenol, β-glucan, and fat in EBP were all decreased by 42.33, 8.09, and 59%, respectively. The content of free phenol and β-glucan in FBP was increased by 35.47 and 212.53%, the content of fat in FBP was decreased by 70.88%. The phenol and β-glucan content of FBP was significantly higher than that of BP and EBP, whereas the content of fat was lower than that in EBP, so FBP has greater significance in a healthy diet. The decrease of fat content and increase of β-glucan content in cereal can reduce its GI (glycemic index), which can be used in healthy food to reduce cholesterol, blood sugar, and blood fat of the human body.

TABLE 2

Table 2. Main nutritional and function component of BP, EBP, and FBP.

The phenolic compounds are decomposed by high temperatures and the complex polyphenols are decomposed into other phenolic or non-phenolic compounds during extrusion, therefore, there is a loss of free phenol content. It is reported that there is a significant loss of total phenolics during the extrusion of oat cereals (14). These reports corroborate well with the findings of the present study. The increase of free phenol content after fermentation was mainly due to the acid hydrolysis produced by Lactobacillus fermentation that led to the release of the bound compound into a free state. Similarly, it found that the lactic acid bacteria fermentation increased the contents of the free, soluble conjugate, and total phenolics by 90.7, 17.2, and 37.1% respectively (15). Mao et al. (16) found that the total phenol content was increased significantly after fermentation, which was 39.10% higher than the raw wheat bran.

Tosh et al. (17) found that the depolymerization of the cell wall caused by pressure during the extrusion process leads to the loss of integrity, resulting in a decrease in the content of β-glucan in cereals. Similarly, it has been reported that it exhibited a significant decrease of 8% in β-glucan content when the extrusion temperature was constant at 150°C, and when extrusion was carried out at 170°C, β-glucan content lowered by 10% (18). The apparent increase in soluble β-glucan observed after fermentation might be due to enhanced activity of enzymes like β-glucanases and carboxypeptidases which cause degradation of total and insoluble β-glucan content into soluble β-glucan (4).

The decrease of the fat content of EBP might be due to the formation of lipid complexes under high temperatures and pressures. Hagenimana et al. (19) reported that decreases in the fat, protein, and reducing sugar content occurred through the many chemical and structural transformations such as starch gelatinization, protein denaturation, and complex formation between amylose and lipids during the extrusion process. Similarly, the fat content of white and red Ginseng samples significantly decreased from 1.29 to 0.23% after extrusion (20). The apparent decrease of fat content in FBP may be due to the degradation of lipids by lipase during fermentation, which is in agreement with what has been reported by Al-Ansi et al. (21). Besides, it is reported that the endo amylase played a vital role in starch hydrolysis during the fermentation process (22). Similarly, Chai et al. (23) found that the fat content of the Rambutan seed reduced by 22% whereas its free fatty acid content increased by 4.3 folds after 10 days of fermentation.

The Content of Amino Acids

As shown in Table 3, the most abundant amino acids in barley are glutamine, valine, leucine, phenylalanine, and proline. The contents of most amino acids decreased after extrusions, such as glutamic acid, leucine, and arginine, which may be due to the mutual conversion and decomposition of amino acids into small molecules of ammonia under the influence of high temperature and high pressure during extrusion. Similarly, the methionine and cysteine content of peas decreased after extrusion treatment (24).

TABLE 3

Table 3. Free amino acids content (%) of BP, EBP, and FBP.

The proportion of essential amino acids in FBP had increased, such as glutamic acid, glycine, alanine, and methionine. It may be due to the mutual conversion of amino acids during the fermentation of Lactobacillus plantarum. LAB fermentation can activate endogenous protease activity of barley powder by decreasing pH (25), thereby hydrolyzing protein and increasing free amino acid content. Additionally, proteases produced by microorganisms during fermentation could also hydrolyze the protein of wheat bran (26). Similarly, Hu et al. (5) found that the proportion of essential amino acids increased after fermentation, such as threonine, valine, and isoleucine Dallagnol. Mao et al. (16) found that methionine and cysteine of wheat bran were increased by 315 and 33%, respectively, after fermentation, the contents of tyrosine, phenylalanine, isoleucine, Val, and other essential amino acids were also significantly increased.

Effects of Extrusion and Fermentation on Physicochemical Properties

Color

Color is an important sensory quality of barley powder. The brightness (L^*), red–green (a^*), and yellow–blue (b^*) of the samples are shown in Table 4. In general, the L^* value of EBP decreases, while a^* and b^* values increase, L^* and b^* values of FBP decrease, while a^* value increases. The BP was brighter than the EBP, which was brighter than the FBP. The EBP was greener than the FBP, which was greener than the BP. The EBP was bluer than the BP, which was bluer than the FBP.

TABLE 4

Table 4. Color of BP, EBP, and FBP.

The observed change in color of EBP may be attributed to the gelatinization caused by high temperature and high-pressure during extrusion. Reduced sugars and proteins (amino acids) in grain can react under high processing temperatures to promote non-enzymatic browning (Maillard reaction), which results in the darkening of the EBP (27). Similarly, previous researchers have observed that extrusion of the whey protein concentrate and corn starch resulted in higher color differences with the increase in the amylose content (28). The color change of FBP is also related to the browning reaction where a fluctuation in L^* values and an increase in a^* values indicate more browning (29). Similarly, previous researchers have observed that there were significant differences in a^* and b^* values obtained between unfermented and fermented seeds of rambutan (30).

WSI and WAI

The water solubility index (WSI) of barley powder is related to the degree of starch degradation. As shown in Table 5, the WSI was significantly increased from 9.73 (BP) to 10.66% (EBP) after the extrusion of barley powder. It was also found that the WSI of pea flour significantly increased after extrusion from 11.70 to 54.9% (31). It has been reported that the crystalline structure was disrupted, and starch was gelatinized during extrusion so that the amylose leached out to cause the increase in the WSI (32). The WSI of FBP was found to be 18.00%, which was remarkably higher than the BP and EBP. It has been reported that molecular components of starch are degraded during fermentation which results in increased soluble components. Similarly, the gel forming capacity of the fermented rice black gram mix flour was significantly higher than the extrudate products (3).

TABLE 5

Table 5. WSI and WAI of BP, EBP, and FBP.

The WAI reflects the water absorption capacity of barley powder and is significantly dependent on molecular interactions in the crystalline and amorphous regions of the starch granule (33). As shown in Table 2, the WAI of EBP was 462.93% higher than BP. It was also found that the WAI of rice starch significantly increased after extrusion from 136 to 246–412% at different moisture contents (32).

Pasting Properties

Pasting properties are very important in the process of choosing new food manufacturing additives, such as thickeners, emulsifiers, binders, or other components related to paste viscosity (34). Therefore, the result may be used for the indirect estimation of the intensity of the extrusion and fermentation treatment of barley powder. As shown in Table 6, the PV, TV, FV, SB, PT, and PTT of BP are higher than EBP and EBP, which indicated that the BP showed more stable viscosity properties during measurements. The starch pasting levels of EBP and FBP were increased, which could effectively optimize the taste of barley products, and further facilitate the development of barley edible products.

TABLE 6

Table 6. Pasting properties of BP, EBP, and FBP.

The peak viscosity of samples is influenced by amylose content, starch leaching, as well as the content of other components, such as proteins, lipids, and fibers (35). The decrease in PV of EBP may be attributable to starch pasting, protein denaturation, and interactions between starch and protein (36). Additionally, the reduced paste viscosity could be attributed to the formation of compact starch granules during high temperature treatment, which had higher resistance to heat and shear during pasting. The decline in PV of FBP might be caused by amylopectin degradation, disruption of granular structures, and disordering of hierarchical structures of starch (8).

Trough viscosity (TV) indicates the sensitivity of starch granules to shearing. During the 95°C period, swollen granules disintegrated due to the shearing of the paddle and rotating bowl, resulting in a decrease in viscosity known as viscosity breakdown. Final viscosity (FV) indicates a retrogradation tendency of the soluble amylose after cooling, or the ability of the starch paste to form a gel (35). Breakdown (BD) is a characteristic feature of shear resistance and heat resistance of starch hot paste. Setback (SB) reflects the stability and aging trend of starch cold paste, a lower setback value indicates higher stability. The content and structure of Amylose and amylopectin in barley flour can affect the pasting time (PT) and pasting temperature (PTT), higher amylose content, and the longer branched chain of amylopectin cause higher heat to dissolve the crystal and higher PTT.

The decrease in TV, FV, BD, and SB of EBP may be attributable to the speed and temperature of the high extruder screw. Similar observations were reported by Siddiq et al. (37), and Mitrus et al. (38). The reduction in TV, FV, BD, and SB of FBP may be attributable to the higher α-amylase activity; the starch grains tend to lose resistance to swelling, and thus, the viscosity declines (39). Similar observations were reported that the fermentation significantly decreased the pasting properties by Al-Ansi et al. (40) and Zhao et al. (41).

Static Viscoelasticity

As shown in Figure 1, the static rheological properties of BP, EBP, and FBP show the same trend of viscosity change, and the viscosity decreases with the increase of shear rate which might be due to the increasing external force with the increase of shear rate, and the destruction of the hydration structure between polysaccharide molecules and water molecules, leading to the decrease of viscosity. The initial viscosity of EBP is higher than that of BP, and that of FBP is lower than that of BP. The decrease of viscosity is beneficial to improve the palatability of popular products and further promote the development and utilization of barley in the food industry.

FIGURE 1

Figure 1. The static viscoelasticity of BP, EBP, and FBP.

In vitro Digestion of Starch

Starch Decomposition Rate

As can be seen from Figure 2, during the digestion of the sample in vitro, the hydrolysis rate of starch increased rapidly in the first 60 min, especially in the first 20 min. The hydrolysis rate of starch from high to low was EBP, FBP, and BP at 180 min. The results showed that the digestibility of barley powder could be improved by extrusion and fermentation, and it could be rapidly decomposed into reducing sugars in the human body for absorption and utilization. Similar observations were reported by de la Rosa-Millán et al. (42).

FIGURE 2

Figure 2. The starch decomposition rate during digestion of BP, EBP, and FBP.

Contents of TS, RDS, SDS, and RS

The contents of RDS, SDS, and RS in the BP, EBP, and FBP are shown in Table 7. Compared to the BP, EBP, and FBP had less SDS and RS, but more RDS, which indicated that both extrusion and fermentation could accelerate the digestion of the starch by transforming SDS and RS into RDS, significantly improving the digestibility of barley powder, and the effect of EBP was more obvious than that of FBP.

TABLE 7

Table 7. Content of TS, RDS, SDS and RS of BP, EBP, and FBP (%).

Rapidly digestible starch can be quickly digested by the human body, and its content is one of the indicators of digestible foods that can predict elevated glycaemic index. The increase in RDS of EBP could be due to the direct effect of the extrusion treatment on starch granules as BP contains little or no detectable α-amylose inhibitors (43). SDS results in the slow release of glucose into the blood; such property is advantageous for human health as it prevents diabetes and obesity. RS is known as a type of dietary fiber that cannot be digested in the small intestine. The significant increase in RDS of EBP, with a corresponding decrease in the RS, is probably due to the extrusion treatment decreasing starch crystallinity and increasing enzyme availability results in a decrease in RS content. A similar result has been reported by Chen et al. (44).

There was also a significant increase in the RDS content after fermentation from 14.92 to 64.30% and the RS content decreased from 68.66 to 29.11%. Golden found that the significant increase in RDS of FBP, with a corresponding decrease in the RS might be due to the disruption of the ordered structure of starch chains by actions of microbial organisms during fermentation, thereby increasing the accessibility of starch granules to the digestive enzymes (45). A similar result has been reported for the sorghum flour and cassava flour by Inglett et al. (46) and Elkhalifa et al. (47).

In vitro Digestion of Polyphenols

Polyphenols Component

The 7 phenolic compounds from samples were analyzed and are presented in Table 8. According to the HPLC analyses, the total content of each phenolic compound in abstracts from barley powder significantly increased during fermentation while decreased during extrusion. Quantitative results indicated that cumaric acid was the most abundant compound in barley, followed by 2-hydroxybenzoic acid, chlorogenic acid, and 3, 4-dihydroxybenzoic acid, additionally, after in vitro simulation of saliva, gastric, and intestinal digestion.

TABLE 8

Table 8. The content of phenolic compounds before and after digestion in vitro.

Compared to the BP, the content of ferulic acid, 2-hydroxybenzoic acid, and caffeic acid were significantly decreased in EBP. Extrusion could inevitably lead to a decrease of free phenolic acids because of decomposition caused by the high temperature. A similar result has been reported by Zeng et al. (48) for the extruded brown rice and extruded wheat. The contents of chlorogenic acid, p-coumaric acid, and ferulic acid were dramatically increased in FBP. It was widely reported that phenolic acids in cereals were mainly in bound form and involved in linkages of ester, ether, or acetal bonds to cellulose, proteins, or lignin. These linkages break due to the enzymes produced during fermentation such as esterases and proteases. A similar result has been reported for the fermented rice bran (15).

Antioxidant Activity

As shown in Table 9, the DPPH•, ABTS radical cation, and FRAP of BP are higher than those of EBP and lower than those of FBP, indicating that the antioxidant capacity of barley powder was partially lost during the extrusion process and enhanced during the fermentation process. It was observed that there was a positive correlation between the total phenolic content and antioxidant activity.

TABLE 9

Table 9. Antioxidant activity of BP, EBP, and FBP (%).

It had been proven that fermentation could improve the antioxidant activity of barley powder, mainly due to the release of free bioactive compounds caused by biological acidification and microbial enzyme activity (49). A similar result has been reported by Mao et al. (16) for the wheat bran and Chu et al. (50) for the millet bran. Extrusion probably caused the breakdown of phenolic, while fermentation helped to dissolve the phenols as well as improve the extraction efficiency. The increased content of total phenolic contributed to the antioxidant activity and that was consistent with the previous report (51).

Conclusion

The contents of phenols, β-glucan, protein, and lipid decreased after extrusion. A variety of nutrients and the content of essential amino acids increased after fermentation. The antioxidant capacity of EBP was lower than that of BP, and that of FBP was higher than that of BP, which was consistent with the change of phenolic content. Both Maillard reaction during extrusion, and enzymatic browning during fermentation of barley flour can lead to the darkening of the color, and can effectively optimize the palatability of the barley powder by reducing the viscosity. The RDS content of EBP and FBP were higher than that of BP, which indicated that both extrusion and fermentation could improve the digestibility of barley. The results can be used in the application of fermentation and extrusion in the food industry, especially in the production of high digestibility food such as baby rice paste and feed.

Data Availability Statement

The original contributions presented in the study are included in the article/supplementary material, further inquiries can be directed to the corresponding author.

Author Contributions

XX designed the scheme and provided funding acquisition and project administration. JL wrote the main part of the manuscript and participated in the planning and execution of all the experiments. HX assisted in part of the experiment and analyzed it. WT helped with some of the experiments. YZ participated in the design of the experimental scheme. JZ was responsible for writing, reviewing editing, and supervision. All authors read and approved the final manuscript.

Funding

This work was supported by the Yangzhou key research and development project (modern agriculture) [YZ2020043]; Jiangsu Province agricultural independent innovation project [CX (20) 2036].

Conflict of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher's Note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Acknowledgments

Thanks to all authors for their contributions to the experiment.

References

1. Gangopadhyay N, Hossain MB, Rai DK, Brunton NP. A review of extraction and analysis of bioactives in oat and barley and scope for use of novel food processing technologies. Molecules. (2015) 20:10884–909. doi: 10.3390/molecules200610884

PubMed Abstract | CrossRef Full Text | Google Scholar

2. Kariluoto S, Edelmann M, Nyström L, Sontag-Strohm T, Salovaara H, Kivelä R, et al. In situ enrichment of folate by microorganisms in beta-glucan rich oat and barley matrices. Int J Food Microbiol. (2014) 176:38–48. doi: 10.1016/j.ijfoodmicro.2014.01.018

PubMed Abstract | CrossRef Full Text | Google Scholar

3. Rani P, Kumar A, Purohit S, Rao P. Impact of fermentation and extrusion processing on physicochemical, sensory and bioactive properties of rice-black gram mixed flour. LWT - Food Science and Technology. (2017) 89. doi: 10.1016/j.lwt.2017.10.050

CrossRef Full Text | Google Scholar

4. Arora S, Jood S, Khetarpaul N, Goyal R. Effect of germination and fermentation on ph. Titratable acidity and chemical composition of pearl millet based food blends. Acta Alimentaria - ACTA ALIMENT. (2009) 38:107–15. doi: 10.1556/AAlim.2008.0024

CrossRef Full Text | Google Scholar

5. Hu Y, Piao C, Chen Y, Zhou Y, Wang D, Yu H, et al. Soybean residue (okara) fermentation with the yeast Kluyveromyces marxianus. Food Bioscience. (2019) 31:100439. doi: 10.1016/j.fbio.2019.100439

CrossRef Full Text | Google Scholar

6. Morris CF, Jeffers HC, Engle DA. Effect of processing, formula and measurement variables on alkaline noodle color—toward an optimized laboratory system. Cereal Chem. (2000) 77:77–85. doi: 10.1094/CCHEM.2000.77.1.77

PubMed Abstract | CrossRef Full Text | Google Scholar

7. Wang H, Wu J, Luo S, Zou P, Guo B, Liu Y, et al. Improving instant properties of kudzu powder by extrusion treatment and its related mechanism. Food Hydrocoll. (2019) 101:105475. doi: 10.1016/j.foodhyd.2019.105475

CrossRef Full Text | Google Scholar

8. Wang H, Xiao N, Ding J, Zhang Y, Liu X, Zhang H. Effect of germination temperature on hierarchical structures of starch from brown rice and their relation to pasting properties. Int J Biol Macromol. (2020) 147:965–72. doi: 10.1016/j.ijbiomac.2019.10.063

PubMed Abstract | CrossRef Full Text | Google Scholar

9. Horax R, Hettiarachchy N, Islam S. Total phenolic contents and phenolic acid constituents in 4 varieties of bitter melons (Momordica charantia) and antioxidant activities of their extracts. J Food Sci. (2005) 70:C275–80. doi: 10.1111/j.1365-2621.2005.tb07173.x

CrossRef Full Text | Google Scholar

10. Minekus M, Alminger M, Alvito P, Ballance S, Bohn T, Bourlieu C, et al. A standardised static in vitro digestion method suitable for food - an international consensus. Food Funct. (2014) 5:1113–24. doi: 10.1039/C3FO60702J

PubMed Abstract | CrossRef Full Text | Google Scholar

11. Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology. (1995) 28:25–30. doi: 10.1016/S0023-6438(95)80008-5

CrossRef Full Text | Google Scholar

12. Sun Y, Qin Y, Li H, Peng H, Chen H, Xie HR, et al. Rapid characterization of chemical constituents in Radix Tetrastigma, a functional herbal mixture, before and after metabolism and their antioxidant/antiproliferative activities. J Funct Foods. (2015) 18:300–18. doi: 10.1016/j.jff.2015.07.009

CrossRef Full Text | Google Scholar

13. Benzie IF, Strain JJ. Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods Enzymol. (1999) 299:15–27. doi: 10.1016/S0076-6879(99)99005-5

PubMed Abstract | CrossRef Full Text | Google Scholar

14. Viscidi K, Dougherty M, Briggs J, Camire M. Complex phenolic compounds reduce lipid oxidation in extruded oat cereals. LWT - Food Sci Technol. (2004) 37:789–96. doi: 10.1016/j.lwt.2004.03.005

CrossRef Full Text | Google Scholar

15. Liu L, Zhang R, Deng Y, Zhang Y, Xiao J, Huang F, et al. Fermentation and complex enzyme hydrolysis enhance total phenolics and antioxidant activity of aqueous solution from rice bran pretreated by steaming with α-amylase. Food Chem. (2017) 221:636–43. doi: 10.1016/j.foodchem.2016.11.126

PubMed Abstract | CrossRef Full Text | Google Scholar

16. Mao M, Wang P, Shi K, Bie X, Zhao H, Zhan C, et al. Effect of solid state fermentation by Enterococcus faecalis M2 on antioxidant and nutritional properties of wheat bran. J Cereal Sci. (2020) 94:102997. doi: 10.1016/j.jcs.2020.102997

CrossRef Full Text | Google Scholar

17. Tosh SM, Brummer Y, Miller SS, Regand A, Defelice C, Duss R, et al. Processing affects the physicochemical properties of beta-glucan in oat bran cereal. J Agric Food Chem. (2010) 58:7723–30. doi: 10.1021/jf904553u

PubMed Abstract | CrossRef Full Text | Google Scholar

18. Huth M, Dongowski G, Gebhardt E, Flamme W. Functional properties of dietary fibre enriched extrudates from barley. J Cereal Sci. (2000) 32:115–28. doi: 10.1006/jcrs.2000.0330

CrossRef Full Text | Google Scholar

19. Hagenimana A, Ding X, Fang T. Evaluation of rice flour modified by extrusion cooking. J Cereal Sci. (2006) 43:38–46. doi: 10.1016/j.jcs.2005.09.003

CrossRef Full Text | Google Scholar

20. Gui Y, Ryu GH. Effects of extrusion cooking on physicochemical properties of white and red ginseng (powder). J Ginseng Res. (2014) 38:146–53. doi: 10.1016/j.jgr.2013.12.002

PubMed Abstract | CrossRef Full Text | Google Scholar

21. Al-Ansi W, Mahdi A, Al-Maqtari Q, Sajid B, Ahmed A, Karrar E, et al. The potential improvements of naked barley pretreatments on GABA, β-glucan, and antioxidant properties. LWT. (2020) 130:109698. doi: 10.1016/j.lwt.2020.109698

CrossRef Full Text | Google Scholar

22. Kalita D, Sarma B, Srivastava B. Influence of germination conditions on malting potential of low and normal amylose paddy and changes in enzymatic activity and physico chemical properties. Food Chem. (2017) 220:67–75. doi: 10.1016/j.foodchem.2016.09.193

PubMed Abstract | CrossRef Full Text | Google Scholar

23. Chai KF, Adzahan NM, Karim R, Rukayadi Y, Ghazali HM. Fat properties and antinutrient content of rambutan (Nephelium lappaceum L.) seed during solid-state fermentation of rambutan fruit. Food Chem. (2019). 274:808–15. doi: 10.1016/j.foodchem.2018.09.065

PubMed Abstract | CrossRef Full Text | Google Scholar

24. Urbano G., López-Jurado M., Frejnagel S., Gómez-Villalva E., Porres J. M., Frías J., et al. (2005). Nutritional assessment of raw and germinated pea (Pisum sativum L.) protein and carbohydrate by in vitro and in vivo techniques. Nutrition 21, 230–239. doi: 10.1016/j.nut.2004.04.025

PubMed Abstract | CrossRef Full Text | Google Scholar

25. Dallagnol AM, Pescuma M, De Valdez GF, Rollán G. Fermentation of quinoa and wheat slurries by Lactobacillus plantarum CRL 778: proteolytic activity. Appl Microbiol Biotechnol. (2013) 97:3129–40. doi: 10.1007/s00253-012-4520-3

PubMed Abstract | CrossRef Full Text | Google Scholar

26. Capuani A, Behr J, Vogel RF. Influence of lactic acid bacteria on redox status and on proteolytic activity of buckwheat (Fagopyrum esculentum Moench) sourdoughs. Int J Food Microbiol. (2013) 165:148–55. doi: 10.1016/j.ijfoodmicro.2013.04.020

PubMed Abstract | CrossRef Full Text | Google Scholar

27. Nayak B, Berrios Jde J, Powers JR, Tang J. Effect of extrusion on the antioxidant capacity and color attributes of expanded extrudates prepared from purple potato and yellow pea flour mixes. J Food Sci. (2011) 76:C874–883. doi: 10.1111/j.1750-3841.2011.02279.x

PubMed Abstract | CrossRef Full Text | Google Scholar

28. Matthey F, Hanna M. Physical and Functional Properties of Twin-screw Extruded Whey Protein Concentrate–Corn Starch Blends. LWT - Food Sci Technol. (1997) 30:359–66. doi: 10.1006/fstl.1996.0189

CrossRef Full Text | Google Scholar

29. Hawlader MNA, Perera CO, Tian M. Properties of modified atmosphere heat pump dried foods. J Food Eng. (2006) 74:392–401. doi: 10.1016/j.jfoodeng.2005.03.028

CrossRef Full Text | Google Scholar

30. Mehdizadeh S, Lasekan OO, Muhammad K, Baharin B. Variability in the fermentation index, polyphenols and amino acids of seeds of rambutan (Nephelium lappaceum L.) during fermentation. J Food Composit Analy. (2014) 37. doi: 10.1016/j.jfca.2014.06.017

CrossRef Full Text | Google Scholar

31. Kristiawan M, Micard V, Maladira P, Alchamieh C, Maigret JE, Réguerre AL, et al. Multi-scale structural changes of starch and proteins during pea flour extrusion. Food Res Int. (2018) 108:203–15. doi: 10.1016/j.foodres.2018.03.027

PubMed Abstract | CrossRef Full Text | Google Scholar

32. Liu Y, Chen J, Luo S, Li C, Ye J, Liu C, et al. Physicochemical and structural properties of pregelatinized starch prepared by improved extrusion cooking technology. Carbohydr Polym. (2017) 175:265–72. doi: 10.1016/j.carbpol.2017.07.084

PubMed Abstract | CrossRef Full Text | Google Scholar

33. Chen J-Y, Liu J, Tang X, Shen X, Liu S. Correlations between the physical properties and chemical bonds of extruded corn starch enriched with whey protein concentrate. RSC Adv. (2017) 7:11979–86. doi: 10.1039/C6RA26764E

CrossRef Full Text | Google Scholar

34. Natabirwa H, Muyonga JH, Nakimbugwe D, Lungaho M. Physico-chemical properties and extrusion behaviour of selected common bean varieties. J Sci Food Agric. (2018) 98:1492–501. doi: 10.1002/jsfa.8618

PubMed Abstract | CrossRef Full Text | Google Scholar

35. Lopes L, Batista K, Fernandes K, Santiago R. Functional, biochemical and pasting properties of extruded bean (Phaseolus vulgaris) cotyledons. Int J Food Sci Technol. (2012) 47. doi: 10.1111/j.1365-2621.2012.03042.x

CrossRef Full Text | Google Scholar

36. Nkundabombi MG, Nakimbugwe D, Muyonga JH. Effect of processing methods on nutritional, sensory, and physicochemical characteristics of biofortified bean flour. Food Sci Nutr. (2016) 4:384–97. doi: 10.1002/fsn3.301

PubMed Abstract | CrossRef Full Text | Google Scholar

37. Siddiq M, Kelkar S, Harte JB, Dolan KD, Nyombaire G. Functional properties of flour from low-temperature extruded navy and pinto beans (Phaseolus vulgaris L.). LWT - Food Sci Technol. (2013) 50:215–9. doi: 10.1016/j.lwt.2012.05.024

CrossRef Full Text | Google Scholar

38. Mitrus M, Wójtowicz A, Kocira S, Kasprzycka A, Szparaga A, Oniszczuk T, et al. Effect of extrusion-cooking conditions on the pasting properties of extruded white and red bean seeds. Int Agrophys. (2020). 34:25–32. doi: 10.31545/intagr/116388

PubMed Abstract | CrossRef Full Text | Google Scholar

39. Simsek S, Ohm JB, Lu H, Rugg M, Berzonsky W, Alamri MS, et al. Effect of pre-harvest sprouting on physicochemical properties of starch in wheat. Foods. (2014) 3:194–207. doi: 10.3390/foods3020194

PubMed Abstract | CrossRef Full Text | Google Scholar

40. Al-Ansi W, Sajid BM, Mahdi AA, Al-Maqtari QA, Al-Adeeb A, Ahmed A, et al. Molecular structure, morphological, and physicochemical properties of highlands barley starch as affected by natural fermentation. Food Chem. (2021) 356:129665. doi: 10.1016/j.foodchem.2021.129665

PubMed Abstract | CrossRef Full Text | Google Scholar

41. Zhao T, Li X, Zhu R, Ma Z, Liu L, Wang X, et al. Effect of natural fermentation on the structure and physicochemical properties of wheat starch. Carbohydr Polym. (2019) 218:163–9. doi: 10.1016/j.carbpol.2019.04.061

PubMed Abstract | CrossRef Full Text | Google Scholar

42. de la Rosa-Millán J, Heredia-Olea E, Perez-Carrillo E, Guajardo-Flores D, Serna-Saldívar SR. Effect of decortication, germination and extrusion on physicochemical and in vitro protein and starch digestion characteristics of black beans (Phaseolus vulgaris L.). LWT. (2019) 102:330–337. doi: 10.1016/j.lwt.2018.12.039

CrossRef Full Text | Google Scholar

43. Alonso R, Grant G, Dewey P, Marzo F. Nutritional assessment in vitro and in vivo of raw and extruded peas (Pisum sativum L.). J Agric Food Chem. (2000) 48:2286–90. doi: 10.1021/jf000095o

PubMed Abstract | CrossRef Full Text | Google Scholar

44. Chen Y, Ma Y, Dong L, Jia X, Liu L, Huang F, et al. Extrusion and fungal fermentation change the profile and antioxidant activity of free and bound phenolics in rice bran together with the phenolic bioaccessibility. LWT. (2019) 115:108461. doi: 10.1016/j.lwt.2019.108461

CrossRef Full Text | Google Scholar

45. Odey G, Lee W. Evaluation of the quality characteristics of flour and pasta from fermented cassava roots. Int J Food Sci Technol. (2019) 55. doi: 10.1111/ijfs.14364

CrossRef Full Text | Google Scholar

46. Inglett GE, Peterson S, Carriere C, Maneepun S. Rheological, textural, and sensory properties of Asian noodles containing an oat cereal hydrocolloid. Food Chem. (2005) 90:1–8. doi: 10.1016/j.foodchem.2003.08.023

CrossRef Full Text | Google Scholar

47. Elkhalifa AE, Schiffler B, Bernhard R. Effect of fermentation on the starch digestibility, resistant starch and some physicochemical properties of sorghum flour. Nahrung. (2004) 48:91–4. doi: 10.1002/food.200300322

PubMed Abstract | CrossRef Full Text | Google Scholar

48. Zeng Z, Liu C, Luo S, Chen J, Gong E. The profile and bioaccessibility of phenolic compounds in cereals influenced by improved extrusion cooking treatment. PLoS ONE. (2016) 11:e0161086. doi: 10.1371/journal.pone.0161086

PubMed Abstract | CrossRef Full Text | Google Scholar

49. Pontonio E, Dingeo C, Gobbetti M, Rizzello CG. Maize milling by-products: from food wastes to functional ingredients through lactic acid bacteria fermentation. Front Microbiol. (2019) 10:561. doi: 10.3389/fmicb.2019.00561

PubMed Abstract | CrossRef Full Text | Google Scholar

50. Chu J, Zhao H, Lu Z, Lu F, Bie X, Zhang C. Improved physicochemical and functional properties of dietary fiber from millet bran fermented by Bacillus natto. Food Chem. (2019) 294:79–86. doi: 10.1016/j.foodchem.2019.05.035

PubMed Abstract | CrossRef Full Text | Google Scholar

51. Zhu F, Du B, Xu B. Superfine grinding improves functional properties and antioxidant capacities of bran dietary fibre from Qingke (hull-less barley) grown in Qinghai-Tibet Plateau, China. J Cereal Sci. (2015) 65. doi: 10.1016/j.jcs.2015.06.006

CrossRef Full Text | Google Scholar