Corrigendum: What's Normal? Microbiomes in Human Milk and Infant Feces Are Related to Each Other but Vary Geographically: The INSPIRE Study

A Corrigendum on
What's Normal? Microbiomes in Human Milk and Infant Feces Are Related to Each Other but Vary Geographically: The INSPIRE Study

by Lackey, K. A., Williams, J. E., Meehan, C. L., Zachek, J. A., Benda, E. D., Price, W. J., et al. (2019). Front. Nutr. 6:45. doi: 10.3389/fnut.2019.00045

In the original article, there was an error. The volume of milk subjected to DNA extraction was reported as 2.5 mL, which is an error in the decimal placement. The actual volume of milk used was 0.25 mL.

A correction has been made to the Materials and Methods section, subsection Extraction of DNA from Milk, paragraph 2:

“DNA in 0.25 mL of each milk sample collected in ETR was extracted using the kit accompanying the Milk Preservation Solution (Norgen Biotek, Thorold, Ontario) as per manufacturer's instructions, including the 2 h enzymatic lysis (20 mg/mL lysozyme). DNA was eluted in 100 μL elution buffer (included with the kit) and stored at −20°C until amplification. Nuclease-free water (500 μL; Ambion) was extracted as a negative control.”

Additionally, the reports of the sequencing reads in the results section were incorrect. A correction has been made to the Results section, subsection Sequencing Summary:

“The sequencing run for the 398 infant fecal samples generated 4,385,982 reads, with a mean (± standard deviation, SD) of 11,020 ± 6,632 reads and a range of 10 to 40,267 reads following initial processing using the DADA2 workflow. After additional filtering of any read that could not be classified to the genus level, and omitting any sample with <1,000 reads, the infant fecal dataset analyzed here contained 4,314,551 reads across 377 samples, with a mean (± SD) of 11,444 ± 6,198; and a range of 1,662–40,255 reads. For the 409 milk samples, sequencing generated 7,528,193 reads, with a mean (± SD) of 18,406 ± 18,389 reads and a range of 12−141,620 reads following initial processing using the DADA2 workflow. Using the same filtering criteria as the infant fecal dataset, the milk dataset used here contained 6,709,277 reads across 394 samples, with mean (± SD) of 17,029 ± 16,783, and a range of 1,302–130,700 reads. These curated datasets were used for all further analyses.”

The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.