Dedi Kong
Lingxi Kong3†
Jing Wang
Chunfu Dai- 1Department of Otology and Skull Base Surgery, Eye Ear Nose and Throat Hospital, Fudan University, Shanghai, China
- 2Key Laboratory of Hearing Medicine, Ministry of Health, Eye Ear Nose and Throat Hospital, Fudan University, Shanghai, China
- 3Department of Pharmacology, School of Basic Medical Sciences, State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, and Institutes of Brain Science, Fudan University, Shanghai, China
Objective: MVN GABAergic neurons is involved in the rebalance of commissural system contributing to alleviating acute peripheral vestibular dysfunction syndrome. This study aims to depict monosynaptic inputs to MVN GABAergic neurons.
Methods: The modified rabies virus-based retrogradation method combined with the VGAT-IRES-Cre mice was used in this study. Moreover, the commissural connections with MVN GABAergic neurons were analyzed.
Results: We identified 60 nuclei projecting to MVN GABAergic neurons primarily distributed in the cerebellum and the medulla. The uvula-nodulus, gigantocellular reticular nucleus, prepositus nucleus, intermediate reticular nucleus, and three other nuclei sent dense inputs to MVN GABAergic neurons. The medial (fastigial) cerebellar nucleus, dorsal paragigantocellular nucleus, lateral paragigantocellular nucleus and 10 other nuclei sent moderate inputs to MVN GABAergic neurons. Sparse inputs to MVN GABAergic neurons originated from the nucleus of the solitary tract, lateral reticular nucleus, pedunculopontine tegmental nucleus and 37 other nuclei. The MVN GABAergic neurons were regulated by the contralateral MVN, lateral vestibular nucleus, superior vestibular nucleus, and inferior vestibular nucleus.
Conclusion: Our study contributes to further understanding of the vestibular dysfunction in terms of neural circuits and search for new strategies to facilitate vestibular compensation.
1 Introduction
The medial vestibular nuclei (MVN), is a crucial processor of vestibular inputs (1). These inputs primarily originate from crista ampullaries of two lateral semicircular canals (2). The MVN integrates information regarding the head movement in space. In addition, visual, and proprioceptive signals also converge in the MVN (3, 4). The MVN sends ascending axonal fibers to the oculomotor nuclei mediating the vestibuloocular reflex (VOR) and bilateral descending projections to the cervical ventral horn to control the vestibular-spinal reflex. Thus, it is essential in maintaining posture, clear vision and static and dynamic balance (5, 6). Furthermore, it is also involved in cognition, such as navigation, spatial memory and learning (7). Normal vestibular function is essential for daily life. When patients suffer from vestibular dysfunction, they complain acute vestibular syndrome (8). It is characterized by vertigo, gaze instability, vegetative disorders, and cognitive alterations which strongly limit daily activities (9, 10). Certain syndrome can alleviate over time is known as vestibular compensation (11). However, the mechanisms underlying vestibular compensation remain unclear.
In the rhombomeric perspective, the MVN in mouse extends at least from rhombomere r5 to r6. The MVN is comprised of two heterogeneous divisions: small dorsal neurons and larger ventral neurons. Cells in both divisions of the MVN express GAD67 mRNAs which labels cell bodies of GABAergic neurons (12, 13). Previous immunohistochemical studies demonstrated that dorsal neurons in the MVN synthesize gamma-aminobutyric acid (GABA) and are intensely stained by GABA-antibody, supporting a functional GABAergic system exists within the MVN (14–16). GABA is considered as a common inhibitory neurotransmitter within brain (17). Further studies had revealed that GABAergic neurons produced regular firing in electrophysiological recording technology (18–20).
GABAergic neurons within the MVN send axons to the cervical ventral horn and the oculomotor nuclei to mediate the inhibitory influence (21, 22). MVN GABAergic neurons project primarily to the caudal ventrolateral medulla (CVLM) to mediate vestibulosympathetic reflex, showing a target preference (23). Moreover, MVN GABAergic neurons are essential for vestibular compensation by involving in the commissural system between the bilateral MVN (17, 24–26).
Taken together, GABAergic neurons within the MVN are involved in controlling posture, balance, and gaze stabilization, particularly in vestibular compensation. Thus, investigating the afferent inputs to MVN GABAergic neurons will facilitate the search for optional circuits that manipulate GABAergic neurons. The connectivity of MVN neurons have been previously investigated using classic retrograde and anterograde tracers. These studies showed that projections to MVN originated from the dorsal raphe nucleus, inferior olivary, and parabrachial nucleus (27–31). However, specific inputs to MVN GABAergic neurons remain unelucidated. Unlike traditional tracers that cannot distinguish neuron types, the current modified rabies virus (RV) method and the Cre/LoxP system enable to identify specific neurons without affecting passing neural tracts. Accordingly, it allows us to explore neural connectivity of a well-defined neuron type rather than a specific brain region (32–35). In this study, we used modified RV and VGAT-IRES-Cre mice to map out monosynaptic inputs targeting MVN GABAergic neurons.
2 Materials and methods
2.1 Animals
Adult VGAT-IRES-Cre mice and their wild-type littermates were used in this study. All mice were housed under suitable environment (constant temperature: 22 ± 0.5°C and relative humidity: 60% ± 2%) and ensured an adequate supply of food and water. All animal experiments were approved by the Animal Experiments Ethics Committee at Shanghai Public Health Clinical Center, Fudan University.
2.2 Virus
All viruses used in the retrograde tracing study were acquired from BrainVTA (Wuhan, China). rAAV2/9-Ef1α-DIO-EGFP-TVA-WPRE (5 × 1012 genomic copies/mL) and rAAV2/9-Ef1α-DIO-RVG-WPRE (5 × 1012 genomic copies/mL) were combined in equal proportions as the helper virus. And the titer of the RV-ENVA-ΔRG-DsRed (RV) was 2 × 108 genomic copies/mL.
2.3 Virus injection and histological preparation
Virus injection and histological preparation were performed as previously described (32, 33). All mice undergone twice injections of virus injections, respectively. Brief description as following, anesthetized VGAT-IRES-Cre and wild-type mice (pentobarbital sodium, 50 mg/kg, intraperitoneal) were securely positioned on a stereotaxic instrument (RWD Life Science, China). And its skull was aligned to make it parallel to the reference plane. Firstly, 100 nL of the AAV-helper virus mixture were injected into the unilateral MVN (−6.0 mm AP, +0.8 mm ML, −3.2 mm DV). Three weeks afterward, double volume of RV was injected into the same position as before. An additional 10 min of holding the pipette was required to ensure full diffusion of virus particles into the target nuclei.
One week later, the anesthetized mice were perfused with 0.1 M phosphate-buffered saline, then with 4% paraformaldehyde. The brain samples were post-fixed in 4% paraformaldehyde overnight. Subsequently, they were dehydrated in various gradients (10, 20, 30%) of sucrose. Brain samples were coronally sectioned (30-μm thick). All samples were divided into three series.
2.4 Imaging and data analysis
All sections were imaged by virtual-slide microscope (Olympus, Tokyo, Japan). The Olympus analysis software (OlyVIA v.2.9, Tokyo, Japan) and ImageJ software (v.2.1.0, Bethesda, MD, United States) were utilized for detailed analyses. Starter cells were identified by co-expressing DsRed and GFP, whereas afferent neurons only expressed DsRed. Brain structures were recognized based on the standard atlas of mouse brain (36). The neurons labeled with DsRed were counted. To quantify ipsilateral afferent inputs, the input from each nucleus was quantified relative to the total number of input neurons. All data are presented in the form of mean ± standard error of the mean (SEM).
3 Results
3.1 Approaches for identifying monosynaptic inputs to MVN GABAergic neurons
The modified RV-based tracing system was utilized with VGAT-IRES-Cre mice in this study. The helper viruses were Cre-dependent, they can only infect the GABAergic neurons with Cre recombinase. Thus, the enhanced green fluorescent protein (EGFP), avian-specific retroviral receptor (TVA), and the rabies glycoprotein G (RG) were specifically expressed on GABAergic neurons. The modified RV with an avian virus envelope protein (EnvA) only infects neurons with TVA and spread retrogradely with the help of RG. Accordingly, the genetically modified RV retrograde tracing system combined with VGAT-IRES-Cre mice were used to map the afferent inputs to MVN GABAergic neurons (32–35).
On the first day, the helper virus (100 nL) was administered into the unilateral MVN of the wide-type and VGAT-IRES-Cre mice. These Cre-dependent viruses can exclusively infect GABA neurons where the Cre recombinase was present. Then GABA neurons infected with helper virus express TVA-GFP and RG proteins. After 3 weeks, double volume of RV was administered into the previous location. One week later, all mice were sacrificed and perfused (Figure 1).
Figure 1. Experimental strategy for RV-based retrograde tracing in MVN GABAergic neurons. (A) A schematic diagram illustrating the viral vectors and injection steps for virus; (B) A schematic diagram showing the injection site into the MVN of VGAT-IRES-Cre mice.
The starter neurons were described as expressing both GFP and DsRed. Three types of neurons (GFP-labeled neurons, DsRed-labeled neurons, and start neurons labeled by both GAP and DsRed) were observed in the MVN of VGAT-Cre mice. Wild-type littermates without the Cre recombinase were used to verify virus specificity. In the MVN of wild-type mouse, neither GFP nor DsRed-positive cells were found (Figure 2).
Figure 2. Representative images of MVN GABAergic neurons injected with tracing virus. (A) Injection site of the unilateral MVN of VGAT-IRES-Cre mice. (B) Injection site of the unilateral MVN of wide-type mice. (C,D) Representative images displaying starter neurons (yellow), helper viruses-labeled neurons (green) and input neurons (red). The white arrows show the starter neurons.
3.2 Overview of monosynaptic inputs to MVN GABAergic neurons
Serial coronal brain sections were imaged and brain structures were manually recognized by the atlas of mouse brain (36). We discovered that DsRed-labeled neurons were primarily located in the cerebellum and medulla. Only a few DsRed-labeled neurons were observed in the pons, midbrain, hypothalamus, thalamus, and cerebral cortex. Notably, DsRed-labeled neurons were primarily observed in the ipsilateral brain regions (Figure 3). To provide a detailed review of the presynaptic inputs, representative images were selected and enlarged, such as deep mesencephalic nucleus (DpMe), ventrolateral periaqueductal gray (VLPAG), parvicellular reticular nucleus (PCRt), dorsal raphe nucleus (DR), intermediate reticular nucleus (IRt), gigantocellular reticular nucleus (Gi), prepositus nucleus (Pr), locus coeruleus (LC), and dorsal paragigantocellular nucleus (DPGi) (Figure 4).
Figure 3. Representative images of monosynaptic inputs to MVN GABAergic neurons. Brain structures were determined according to the standard mouse atlas. Only the ipsilateral hemisphere was shown. Scale bar: 500 μm. CGPn, central gray of the pons; CnF, cuneiform nucleus; DC, dorsal cochlear nucleus; Dk, nucleus of Darkschewitsch; DMC dorsomedial hypothalamic nucleus, compact part; DMPAG, dorsomedial periaqueductal gray; DPGi, dorsal paragigantocellular nucleus; DpMe, deep mesencephalic nucleus; DR, dorsal raphe nucleus; DRD dorsal raphe nucleus, dorsal part; EW, Edinger-Westphal nucleus; Fl, flocculus; Gi, gigantocellular reticular nucleus; IRt, intermediate reticular nucleus; LC, locus coeruleus; LH, lateral hypothalamic area; LPAG, lateral periaqueductal gray; Med, medial (fastigial) cerebellar nucleus; PH, posterior hypothalamic area; PMnR, paramedian raphe nucleus; PnO, pontine reticular nucleus, oral part; PPTg, pedunculopontine tegmental nucleus; Pr, prepositus nucleus; RPO, rostral periolivary region; VeCb, vestibulocerebellar nucleus; VLPAG, ventrolateral periaqueductal gray.
Figure 4. Schematic images of the functional regions with monosynaptic inputs to MVN GABAergic neurons. Primary inputs to MVN GABAergic neurons originated from brain regions associated with oculomotor controlling [e.g., flocculus, medial (fastigial) cerebellar nucleus and prepositus nucleus], sleep–wake regulation (e.g., dorsal paragigantocellular nucleus, lateral paragigantocellular nucleus and ventrolateral periaqueductal gray) and sympathetic response (e.g., gigantocellular reticular nucleus and intermediate reticular nucleus). Scale bar: 100 μm. CGPn, central gray of the pons; CnF, cuneiform nucleus; Dk, nucleus of Darkschewitsch; DMC,dorsomedial hypothalamic nucleus, compact part; DpG, deep gray layer of the superior colliculus; DPGi, dorsal paragigantocellular nucleus; DpMe, deep mesencephalic nucleus; DRD, dorsal raphe nucleus, dorsal part; DRV, dorsal raphe nucleus, ventral part; DRVL, dorsal raphe nucleus, ventrolateral part; Gi, gigantocellular reticular nucleus; GiA, gigantocellular reticular nucleus, alpha part; InWh, intermediate white layer of the superior colliculus; IRt, intermediate reticular nucleus; LC, locus coeruleus; LDTg, laterodorsal tegmental nucleus; LH, lateral hypothalamic area; LPAG, lateral periaqueductal gray; MPB, medial parabrachial nucleus; PCRt, parvicellular reticular nucleus; PH, posterior hypothalamic area; PnC, pontine reticular nucleus, caudal part; PnO, pontine reticular nucleus, oral part; PPTg, pedunculopontine tegmental nucleus; Pr, prepositus nucleus; RC, raphe cap; Sp5I spinal trigeminal nucleus, interpolar part; VLPAG, ventrolateral periaqueductal gray.
3.3 Commissural connections of GABAergic neurons in the MVN
The contralateral vestibular nuclei complex (VNC), which includes the MVN, superior vestibular nucleus (SVN), lateral vestibular nuclei (LVN) and descending vestibular nucleus (DVN) was observed to reveal the commissural connection (37). The proportion of inputs from subnucleus was calculated as the count of DsRed-labeled cells in each subnucleus divided by the total count of DsRed-labeled cells in VNC. The MVN GABAergic neurons received most inputs from the contralateral MVN (68.54% ± 3.58%), as well as the contralateral DVN (13.68% ± 4.23%), SVN (10.87% ± 0.28%) and LVN (6.90% ± 1.79%) (Figure 5).
Figure 5. Connectivity between MVN GABAergic neurons and the contralateral VNC. (A) (B) Images showing dsRed-labeled neurons in contralateral MVN, LVN, SVN and DVN; (C) Statistical analysis of commissure connection (n = 3). VNC, vestibular nuclei complex; MVN, medial vestibular nucleus; LVN, lateral vestibular nucleus; SVN, superior vestibular nucleus; DVN, descending vestibular nucleus.
3.4 Analysis of afferent neurons providing input to MVN GABAergic neurons
We calculated the radio for each nucleus by dividing the count of DsRed-labeled neurons in a region by the total count of DsRed-labeled neurons ipsilaterally. We identified 60 nuclei projecting to MVN GABAergic neurons, each contributing over 0.1% of the total labeled neurons on the ipsilateral side. And proportions above 3% were defined as dense inputs, between 1 and 3% were defined as moderate inputs, and below 1% were defined as sparse inputs (35).
Dense inputs (>3% of total DsRed-labeled neurons) to MVN GABAergic neurons originated from following nucleus: uvula-nodulus (40.675 ± 6.76%), Gi (6.48% ± 1.31%), Pr, (4.39 ± 1.27%), IRt (3.28% ± 0.93%), pontine reticular nucleus, caudal part (4.10% ± 0.73%), pontine reticular nucleus, oral part (3.28 ± 0.66%), central gray of the pons (3.25% ± 1.49%). Besides, MVN GABAergic neurons also received moderate monosynaptic inputs (more than 1% of total DsRed-labeled neurons) from several nuclei, such as: vestibulocerebellar nucleus, medial (fastigial) cerebellar nucleus (Med), dorsal cochlear nucleus, DPGi, raphe magnus nucleus, spinal trigeminal nucleus, lateral paragigantocellular nucleus (LPGi), PCRt, laterodorsal tegmental nucleus (LDTg), LC, DpMe, VLPAG, DR, lateral periaqueductal gray (LPAG) (Figure 6). A schematic diagram displaying the monosynaptic inputs to the MVN GABAergic neurons is shown in Figure 7.
Figure 6. Statistical analysis of ipsilateral monosynaptic inputs to MVN GABAergic neurons. The average proportion of monosynaptic inputs from brain regions contributing more than 0.1% of the total inputs to MVN GABAergic neurons was analyzed and listed. Brain regions are categorized into seven general structures and presented at the top, Sample size: n = 3. Arc, arcuate hypothalamic nucleus; Bar, Barrington’s nucleus; Au1, primary auditory cortex; CGPn, central gray of the pons; CnF, cuneiform nucleus; Cu, cuneate nucleus; DC, dorsal cochlear nucleus; DM, dorsomedial hypothalamic nucleus; DMPAG, dorsomedial periaqueductal gray; DMTg, dorsomedial tegmental area; DpG, deep gray layer of the superior colliculus; DPGi, dorsal paragigantocellular nucleus; DpMe, deep mesencephalic nucleus; DR, dorsal raphe nucleus; EW, Edinger-Westphal nucleus; Fl, flocculus; Gi, gigantocellular reticular nucleus; IntP, interposed cerebellar nucleus, posterior part; IRt, intermediate reticular nucleus; LC, locus coeruleus; LDTg, laterodorsal tegmental nucleus; LH, lateral hypothalamic area; LPAG, lateral periaqueductal gray; MPB, medial parabrachial nucleus; LPGi, lateral paragigantocellular nucleus; LPO, lateral preoptic area; LRt, lateral reticular nucleus; LVPO, lateroventral periolivary nucleus; M1, primary motor cortex; M2, secondary motor cortex; Med, medial (fastigial) cerebellar nucleus; MPB, medial parabrachial nucleus; Pa6, paraabducens nucleus; PAG, periaqueductal gray; PCGS, paracochlear glial substance; PCRt, parvicellular reticular nucleus; PDTg posterodorsal tegmental nucleus; PMnR, paramedian raphe nucleus; PnC, pontine reticular nucleus, caudal part; PnO, pontine reticular nucleus, oral part; PPTg, pedunculopontine tegmental nucleus; Pr, prepositus nucleus; PR, prerubral field; PSTh, parasubthalamic nucleus; RC, raphe cap; RMg, raphe magnus nucleus; Ro, nucleus of Roller; Rob, raphe obscurus nucleus; RPO, rostral periolivary region; RtTg, reticulotegmental nucleus of the pons; RVL, rostroventrolateral reticular nucleus; S1BF, primary somatosensory cortex, barrel field; Sol, nucleus of the solitary tract; Sp5, spinal trigeminal tract; SPF, subparafascicular thalamic nucleus; SubC, subcoeruleus nucleus; VeCb, vestibulocerebellar nucleus; VLPAG, ventrolateral periaqueductal gray; ZI, zona incerta.
Figure 7. Schematic illustration showing the distribution of monosynaptic inputs to MVN GABAergic neurons. This figure provides a schematic illustration of the distribution patterns of monosynaptic inputs to MVN GABAergic neurons. The color density represents the amount of input neurons. CGPn, central gray of the pons; DC, dorsal cochlear nucleus; DMTg, dorsomedial tegmental area; DPGi, dorsal paragigantocellular nucleus; DpMe, deep mesencephalic nucleus; DR, dorsal raphe nucleus; Gi, gigantocellular reticular nucleus; IRt, intermediate reticular nucleus; LC, locus coeruleus; LDTg, laterodorsal tegmental nucleus; LPGi, lateral paragigantocellular nucleus; Med, medial (fastigial) cerebellar nucleus; PCRt, parvicellular reticular nucleus; PnC, pontine reticular nucleus, caudal part; PnO, pontine reticular nucleus, oral part; Pr, prepositus nucleus; RMg, raphe magnus nucleus; Sp5, spinal trigeminal tract; VeCb, vestibulocerebellar nucleus; VLPAG, ventrolateral periaqueductal gray.
4 Discussion
To gain a deeper insight of how MVN GABAergic neurons mediate physiological behaviors, it is necessary to explore the monosynaptic inputs to them which modulate their activity. In this study, a modified RV-based tracing system and VGAT-Cre mice were utilized. Our results revealed the presynaptic inputs to MVN GABAergic neurons, providing insight into the mechanisms mediating their activity. Additionally, we explored the commissural system and found that MVN GABAergic neurons are influenced by inputs from the contralateral MVN, LVN, SVN and DVN. These findings contribute to understanding commissure system and providing strategies to facilitate vestibular compensation.
4.1 Comparison with earlier tracing studies
Previous research in rats has revealed connectivity between the MVN and DR by using both the anterograde transport of biotinylated dextran amine and retrograde transport of Fluoro-Gold (27). This pathway was also confirmed on mice in our study. The traditional retrograde method using horseradish peroxidase showed the inferior olive (IO) projects to the MVN in rabbits (28, 30). However, we did not find specific inputs from the IO to the MVN GABAergic neurons, suggesting the IO may project to other neuron types of the MVN. This highlighted a limitation of traditional tracer methods which cannot identify cell type-specific neurons in the target nucleus. Genetically modified RV has been extensively used in anatomical studies, particularly in neurosciences, due to its effectiveness in labeling presynaptic inputs of defined neuronal cell-types in transgenic mice (38, 39).
To address this limitation, our group previously used the RV retrograde tracing system to investigate the monosynaptic inputs to GABAergic neurons in the VNC (32). However, heterogeneous subnuclei which performed distinct functions and commissure connections which attributed to vestibular compensation were not considered. In this study, we focused on the MVN, the largest subnucleus of the VNC. The RV-based retrograde system combined with VGAT-IRES-Cre mice was utilized to investigate the presynaptic inputs to GABAergic neurons of the MVN in this study. We discovered 60 upstream nuclei that innervated MVN GABAergic neurons, as well as inputs from the contralateral VNC that formed the commissural system. In conclusion, our study offered a more detailed and systematic mapping of inputs to MVN GABAergic neurons.
4.2 Implications for MVN GABAergic neurons in physiological behavior
The MVN neurons bilaterally travel through the medial longitudinal fasciculus to the medial ventral horn of the cervical cord. These neurons control the contraction of neck muscles to adjust the head and neck movements to maintain balance forming the vestibulospinal reflex. The MVN send ascending fibers to the ipsilateral oculomotor nucleus (CN 3) and contralateral abducens nucleus (CN 6) along with the SVN mediating the vestibuloocular reflex. This coordinate horizontal eye movements (40, 41).
Increased evidences have shown that the neurons connecting the MVN and the oculomotor nucleus was GABAergic, and these GABAergic neurons were also regulated by brain regions associated with oculomotor control (42, 43).
Results of this study confirmed this finding. The cerebellum gains direct projections from the vestibular end-organs and project to the MVN, acting as an adaptive processor (44–46). Direct inputs from the flocculus (Fl) and uvula-nodulus to the MVN have been revealed in cats and rabbits (1, 47–50). The cerebellum regulated the MVN through inhibitory inputs. Different regions projecting to MVN GABAergic neurons played distinct roles in regulating VOR. The unipolar brush cells within the uvula-nodulus receive vestibular inputs via mossy fibers from the vestibular end-organs and the vestibular nuclei. As feedback, these cells mediate the activity of the mossy fibers to control the vestibular inputs (51–55). Previous studies have shown damage of the uvula-nodulus affected the speed of the slow phase of eye movements relative to the head position, rather than the spatial orientation of the nystagmus (56, 57). Unlike the uvula-nodulus, the flocculus participated in the gain of the VOR (58). The Pr integrated the velocity and position signals of horizontal eye movements to maintain stable gaze (59). Researches in monkeys and humans have revealed the lesions of Pr results in defects in maintaining stable gaze (60–62). These indicated the uvula-nodulus, flocculus, and Pr are crucial components of the VOR circuits.
Additionally, sleep–wake system and vestibular system also interact. Clinically, patients with vestibular dysfunction often exhibit sleep disturbances, however, activation by electricity or rocking movements of the vestibular system can facilitate non-rapid eye movement (NREM) sleep (63–66). Franken and his colleagues found NREM sleep was increased and wakefulness episodes were shortened through stimulating the vestibular system by rocking movements at 1.0 Hz (67). Further studies revealed that neurotensinergic neurons in the MVN promoted NREM sleep, and these neurons were primarily GABAergic (68). By contrast, Yanagisawa et al. found that GABAergic neurons in the lateral MVN contributed to stabilizing wakefulness and regulating the transition into rapid eye movement (REM) sleep based on vestibular information (42). This may be reasonable because MVN GABAergic neurons were linked to various brain regions involved in not only improving sleep but also developing wakefulness. Likewise, MVN GABAergic neurons received direct projections from brain area related to sleep/wake cycle control. The LC and DR have been demonstrated to facilitate arousal (69, 70). Previous experiments showed there are projections from LC and DR to the vestibular nuclei (71, 72). In the present study, we further revealed the LC and DR send moderate projections to MVN GABAergic neurons. Inputs from the LC and DR can influence the gain of the vestibular reflexes and cerebellar-vestibular pathway, respectively (27, 73–75). In addition, afferent inputs to MVN GABAergic neurons also arose from NREM sleep-developing brain structures, such as the VLPAG and DpMe. The excitation of VLPAG GABAergic neurons increased NREM sleep and decreased REM sleep (76, 77). Chen et al. revealed that exciting GABAergic neurons in the dorsal part of DpMe promoted NREM sleep via the sublaterodorsal nucleus pathway (78). Brain nuclei that enhance REM sleep, such as the DPGi, LPGi, and LDTG, were found to send moderate inputs to the MVN GABAergic neurons in this study. DPGi GABAergic neurons enhanced REM sleep through the suppression of the LC and DR (79–81). Similarly, LPGi may hyperpolarize REM-off neurons in the LC to generate REM sleep (82). Electrical stimulation of LDTG also increased REM sleep (83).
GABAergic neurons in the MVN also participate in the vestibulosympathetic reflex to moderate blood distribution during postural change and movement. The MVN GABAergic neurons projected primarily to the caudal ventrolateral medulla (CVLM) which influenced sympathetic nerve activity by influencing the rostral ventrolateral medulla (23, 84). MVN GABAergic neurons receive feedback signals from sympathetic-related brain structures, such as the Gi and IRt. Kuo et al. found that activation of certain regions of the Gi induced a decrease in heart rate and caused hypotension in cats (85). The IRt served as a hub transmitting post-inspiratory activity to sympathetic and motor outputs (86, 87).
Our results revealed that MVN GABAergic neurons integrated multisensory signals related to oculomotor control, sleep/wakefulness regulation, and sympathetic responses. These findings established a basis for deeper investigation into the neural pathways mediating the physiological functions of MVN GABA neurons.
4.3 Implications for MVN GABAergic neurons in vestibular compensation
Normal vestibular system is essential for visual stabilization, postural maintenance, and equilibrium control, by relying on symmetrical afferent inputs to the vestibular nuclei (88). Several researches have shown that there are inter-nuclear connections between the bilateral vestibular nuclei (20, 89, 90). The inhibitory commissural system linking the MVN and its contralateral counterpart is fundamental to complete vestibular reflexes (91). Partial or total interruption of unilateral inputs, such as unilateral vestibular deafferentation (UVD) and unilateral labyrinthectomy led to postural and oculomotor deficits (92–94). These deficits were induced by the imbalance in activity between bilateral MVNs (95). The resting discharges of neurons in the ipsilesional MVN were almost silenced, whereas the contralesional MVN neurons became hyperactive (92, 96, 97). Another study demonstrated the resting potential of MVN neurons only decreased by 50% compared to normal situation after bilateral labyrinthectomy (98). These findings indicated that the silence of ipsilesional MVN neurons was primarily caused by enhanced suppression from contralesional MVN neurons (92). The vestibular dysfunction was characterized by static (without movement) and dynamic symptoms (with movement) (96). Static symptoms gradually disappeared within days known as vestibular compensation (91, 96, 99). Inhibitory commissural connections were crucial for the recovery of spontaneous resting potential of the lesioned side and rebalancing neural discharge between the bilateral MVN during vestibular compensation (91, 96).
Our findings showed that GABAergic neurons in the MVN were heavily innervated by projections from the contralateral MVN as well as the contralateral LVN, SVN, and DVN. These patterns were similar to the connections observed in hamsters, in contrast to the commissural connections in cats and monkeys showing afferents to the MVN arising from all parts of contralateral MVN, parts of contralateral SVN and DVN (71, 100). These discrepancies may be due to the differences between species. The commissural system to MVN GABAergic neurons revealed in the present study suggests that these neurons may be regulated by contralateral VNC to achieve bilateral balance, which was crucial for normal vestibular function.
In conclusion, we illustrated monosynaptic inputs to MVN GABAergic neurons. It suggested that MVN GABAergic neurons received information from various brain regions. This finding underscores the crucial role of MVN GABAergic neurons in integrating multiple signals. In addition, the confirmation of the commissure system provides provided evidences that MVN GABAergic neurons were involved in facilitating vestibular compensation.
Data availability statement
The raw data supporting the conclusions of this article will be provided by corresponding author without reservation.
Ethics statement
The animal study was approved by Animal Experiments Ethics Committee at Shanghai Public Health Clinical Center, Fudan University. The study was conducted in accordance with the local legislation and institutional requirements.
Author contributions
DK: Investigation, Methodology, Software, Writing – original draft. LK: Data curation, Investigation, Writing – original draft. CL: Investigation, Writing – original draft. QW: Methodology, Writing – original draft. JW: Methodology, Supervision, Validation, Writing – original draft. CD: Supervision, Writing – review & editing.
Funding
The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. This work was supported by National Natural Science Foundation of China (Nos. 82371143 and 82171142 CFD) and Youth Program of National Natural Science Foundation of China (82201282).
Acknowledgments
We thank all members of Zhili Huang’s lab at Fudan University, Shanghai.
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s note
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Keywords: vestibular function, vestibular disorders, vestibular compensation, medial vestibular nucleus, GABAergic neurons
Citation: Kong D, Kong L, Liu C, Wu Q, Wang J and Dai C (2024) Commissural and monosynaptic inputs to medial vestibular nucleus GABAergic neurons in mice. Front. Neurol. 15:1484488. doi: 10.3389/fneur.2024.1484488
Edited by:
Sulin Zhang, Huazhong University of Science and Technology, ChinaReviewed by:
Viviana Mucci, Western Sydney University, AustraliaBernd Fritzsch, University of Nebraska Medical Center, United States
Qing Zhang, Shanghai Jiaotong University School of Medicine, China
Jun Wang, The First Affiliated Hospital of Nanchang University, China
Copyright © 2024 Kong, Kong, Liu, Wu, Wang and Dai. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Jing Wang, wangjing8597@126.com; Chunfu Dai, cfdai66@126.com
†These authors have contributed equally to this work