This article is a correction to:
Identification and Characterization of Thermostable Y-Family DNA Polymerases η, ι, κ and Rev1 From a Lower Eukaryote, Thermomyces lanuginosus
Alexandra Vaisman1†§John P. McDonald1†§Mallory R. Smith1†Sender L. Aspelund1†‡Thomas C. Evans Jr2†
Roger Woodgate1*†- 1Laboratory of Genomic Integrity, National Institute of Child Health and Human Development, National Institutes of Health, 9800 Medical Center Drive, Bethesda, MD, United States
- 2New England Biolabs Incorporated, Ipswich, MA, United States
A Corrigendum on
Identification and Characterization of Thermostable Y-Family DNA Polymerases η, ι, κ and Rev1 From a Lower Eukaryote, Thermomyces lanuginosus
by Vaisman A, McDonald JP, Smith MR, Aspelund SL, Evans TC and Woodgate R (2021). Front. Mol. Biosci. 8:778400. doi: 10.3389/fmolb.2021.778400
In the original article, there was a formatting issue in Figure 6 as published. This occurred when the image was converted from a PC generated pdf to an Apple Macintosh generated tif for publication. The corrected Figure 6 appears below.
FIGURE 6
FIGURE 6. TLS past trans-S-BPDE-dA by T. lanuginosus pols. The ability to bypass BPDE-dA was assayed for (A) polη in thepresence of 4 mM Mg2+, (B) polκ in the presence of 4 mM Mg2+, (C) polη in the presence of 4 mM Mn2+, (D) polκ in the presence of 4 mM Mn2+, (E) polι in the presence of 4 mM Mn2+, and (F) individual, or a mixture of various pols in 4 mM Mn2+. The substrate used in these assays was made by annealing of the 32P labeled primer 5′-CACTGCAGACTCTAAA-3′ and either an undamaged or BPDE-containing template 5′- GCTCGTCAGCAGATTTAGAGTCTGCAGTG-3′, where the underlined bold A stands for the undamaged, or BPDE modified dA. Reactions contained 100 μM each of individual nucleotide (dC, dG, dA, and dT) or a mixture of all four dNTPs as indicated in the figure and were carried out at 37°C for 10 min. Concentrations of enzymes were 0.17 pM for polη, 0.32 pM for polκ, and 0.15 pM for polι. The sequence of the template immediately downstream of the primer (pr) is shown on the left-hand side of each gel pair. The star (*) indicates the position of the adduct.
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
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Keywords: thermostable fungi, Y-family DNA polymerases, phylogenetic analysis, translesion DNA synthesis, DNA polymerase η, DNA polymerase ι, DNA polymerase κ, REV1
Citation: Vaisman A, McDonald JP, Smith MR, Aspelund SL, Evans TC and Woodgate R (2021) Corrigendum: Identification and Characterization of Thermostable Y-Family DNA Polymerases η, ι, κ and Rev1 From a Lower Eukaryote, Thermomyces lanuginosus. Front. Mol. Biosci. 8:819157. doi: 10.3389/fmolb.2021.819157
Received: 20 November 2021; Accepted: 22 November 2021;
Published: 15 December 2021.
Approved by:
Frontiers Editorial Office, Frontiers Media SA, SwitzerlandCopyright © 2021 Vaisman, McDonald, Smith, Aspelund, Evans and Woodgate. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Roger Woodgate, woodgate@nih.gov
§These authors share first authorship
†ORCID: Alexandra Vaisman, orcid.org/0000-0002-2521-1467; John P. McDonald, orcid.org/0000-0003-2482-148X; Mallory R. Smith, orcid.org/0000-0003-1450-7825; Sender L. Aspelund, orcid.org/0000-0003-0726-4028; Thomas C. Evans Jr., orcid.org/0000-0001-5406-0146; Roger Woodgate, orcid.org/0000-0002-2521-1467
‡Present address: Sender L. Aspelund, Novavax, Inc., Gaithersburg, MD, United States