Cardiac and vascular effects of low-dose steroids during the early phase of septic shock: An echocardiographic study

Background: Low-dose steroids are known to increase arterial pressure during septic shock through restoration of vasopressor response to norepinephrine. However, their effects on cardiac performance and ventriculo-arterial coupling (VAC) have never been scrutinized during human septic shock. The aim of this study was to perform a comprehensive description of the cardiovascular effects of low-dose steroids using modern echocardiographic tools (including speckle tracking imaging).

Methods: This prospective study was conducted in the intensive care unit (ICU) of a university hospital in France. Consecutive adult patients admitted for septic shock and requiring low-dose steroid therapy were prospectively enrolled within 24 h of septic shock onset. We recorded hemodynamic and echocardiographic data to explore left ventricle (LV) contractility, loading conditions and VAC just before the initiation of low-dose steroids (50 mg intravenous hydrocortisone plus 50 μg enteral fludrocortisone) and 2–4 h after.

Results: Fifty patients [65 (55–73) years; 33 men] were enrolled. Arterial pressure, heart rate, almost all LV afterload parameters, and most cardiac contractility parameters significantly improved after steroids. VAC improved with steroid therapy and less patients had uncoupled VAC (> 1.36) after (24%) than before (44%) treatment.

Conclusion: In this comprehensive echocardiographic study, we confirmed an improvement of LV afterload after initiation of low-dose steroids. We also observed an increase in LV contractility with improved cardiovascular efficiency (less uncoupling with decreased VAC).

Introduction

Relative adrenal insufficiency, also appraised as critical illness-related corticosteroid insufficiency is common during septic shock. It is the main pathophysiological rationale for the use of low-dose corticosteroid therapy in septic shock (1). The anti-inflammatory (2) and vasoactive (3) properties of corticosteroids have been well known for several decades. Several studies have demonstrated the peripheral vascular effects of low-dose steroids in septic shock, including an increase in mean arterial pressure (MAP) through restoration of systemic vascular resistance and enhanced vasopressor response to norepinephrine (4–8).

In addition to vasoplegia, septic shock is also characterized by myocardial dysfunction, with a major interaction between contractility and loading conditions (9). Animal models of sepsis suggest a protective role for low-dose hydrocortisone in myocardial injury (10). However, the effect of low-dose steroids on cardiac performance has never been scrutinized during human septic shock. We hypothesized that low-dose steroid therapy may enhance cardiac contractility in addition to its vasopressive effects during human septic shock. Given the strong interaction between left ventricle (LV) contractility and loading conditions during human septic shock (9, 11), it is crucial to assess all components of cardiac performance in this setting. The aim of the present study was to perform a comprehensive description of the effect of low-dose steroid therapy on cardiac and vascular function (contractility, preload, and afterload, with their interactions) during human septic shock, using modern echocardiographic tools.

Materials and methods

Patients

We conducted a prospective observational monocenter study in the medical intensive care unit (ICU) of Henri Mondor University Hospital, Créteil, France, between August 8, 2018 and May 29, 2021. All consecutive patients admitted during this period for septic shock (as defined according to the American College of Chest Physicians (ACCP)/Society of Critical Care Medicine (SCCM) Consensus Conference (12) and requiring low-dose steroid therapy, according to guidelines (13, 14), were prospectively enrolled within 24 h of septic shock onset. Non-inclusion criteria were age less than 18 years, pregnancy, patients under protection (guardianship, curators or safeguard of justice), chronic heart failure with reduced left ventricular ejection fraction (LVEF < 45%) documented in patient’s history, severe valvular disease and prior corticosteroid therapy.

The study was approved on July 12, 2018 by the institutional ethics committee, Institutional Review Board (IRB) Mondor (reference number: 2201948) as a component of standard care, and patient consent was waived, as per French law. Written and oral information about the study was given to the families.

Treatment protocol

Norepinephrine was the first-choice vasopressor therapy (used to target a MAP of 65 mmHg or more); dobutamine was added, based on physician decision, in the presence of decreased LVEF (<45%) with ongoing signs of hypoperfusion despite adequate MAP and correction of hypovolemia with absence of fluid responsiveness. Catecholamine dose and fluid management were kept constant between the two ultrasound assessments, unless significant hemodynamic instability; patients who required a change in norepinephrine dose and/or fluid loading during the study protocol were secondarily excluded. The steroid replacement therapy consisted of hydrocortisone 50 mg intravenously every 6 h plus enteral fludrocortisone (50 μg/day). The patients were followed up until ICU discharge.

Echocardiography

Transthoracic echocardiography (TTE) was performed in early phase of septic shock after hemodynamic stabilization by trained operators (competent in advanced critical care echocardiography) (15). The TTE studies were performed on a GE Vivid S7 or E9 ultrasound system (GEMS, Buc, France), with a 1.5–4.5 MHz (M5S-D) transducer. The first TTE was performed at the initiation of steroid replacement therapy (just before first intravenous bolus of hydrocortisone 50 mg plus enteral fludrocortisone 50 μg). The second TTE was performed 2–4 h after the initiation of low-dose steroid therapy. The echocardiographic results were reported based on the Preferred Reporting Items for Critical care Echocardiography Studies (PRICES) statement of the European Society of Intensive Care Medicine (16). All individual measurements were averaged over a minimum of three cardiac cycles (five to ten in case of non-sinus rhythm) and collected at end-expiration.

Assessment of contractility and loading conditions

Preload

Preload was assessed using estimates of LV filling pressures [E/A and E/e’ ratios from pulsed-wave Doppler early (E) and late (A) and tissue Doppler early (e’) diastolic wave velocity at the lateral and septal mitral valve annulus] (17) and respiratory variations of velocity time integral (VTI) of the left ventricle outflow tract (LVOT) as a surrogate of fluid responsiveness (18, 19).

Afterload

Afterload was assessed using the following indices: (i) diastolic arterial pressure (DAP) which is often used as a surrogate of LV afterload in clinical practice (20); (ii) systemic vascular resistance (the most commonly used measure of vascular tone) (21) = $\frac{80*meanarterialpressure(mmHg)}{cardiacoutput(L.mi{n}^{-1})}$; (iii) end-systolic arterial elastance (E_a), to reflect the pulsatile component of peripheral load (22) = $\frac{0.9*systolicarterialpressure\left(mmHg\right)}{strokevolume(mL)}$; (iv) diastolic shock index (DSI), that could reflect the severity of circulatory dysfunction during vasodilatory conditions = $\frac{heartrate(bpm)}{diastolicarterialpressure(mmHg)}$) (23); and (v) LV end-systolic wall stress (to reflect the combined effects of peripheral loading conditions and factors internal to the heart according to Laplace’s principle) = $\frac{Systolicarterialpressure\left(mmHg\right).leftventricleend-systolicvolume(mL)}{1000}$ (21).

Systolic function and left ventricular contractility

LV systolic function was assessed using indices obtained from the following techniques: (i) two-dimensional echocardiography, with LVEF (computed from LV volume using bi-plane Simpson method (24) when image quality was good, or visually estimated when poor image quality did not allow sufficient identification of the endocardium) (25); (ii) tissue Doppler imaging, with peak systolic wave (s’) at the lateral and septal mitral valve annulus (26); (iii) speckle tracking imaging, with LV global longitudinal peak systolic strain (LV-GLS), derived from apical long-axis (three-, four,- and two-chamber) clips obtained with a frame rate ≥ 50 Hz whenever possible with on-line analyses conducted by two trained operators on the semi-automated EchoPAC package (GEMS, Buc, France); (iv) the LV end-systolic maximal elastance estimated by the modified single-beat method [E_es(sb)], as proposed by Chen et al. (27).

Description of left ventricle end-systolic maximal elastance using single-beat method

The LV end-systolic maximal elastance (E_es) is a major determinant of the LV systolic performance and the interaction between heart and vascular system. E_es corresponds to the slope of the end-systolic pressure-volume relation, which is classically invasively determined by catheter with LV pressures and volumes recorded under different cardiac loading conditions. Several alternatives have been proposed for estimating E_es without loading interventions, and these are generally referred to as single-beat methods [E_es(sb)]. The algorithm for E_es(sb) proposed by Chen et al. (27) is based on the following steps and principles:

(a) If we consider LV elastances during isovolumic contraction [E_d = P_d/(V_d-V₀)] and at end systole [E_es(sb) = P_es/(V_es-V₀)], their ratio [E_Nd = E_d/E_es(sb)], called the time and amplitude normalized time varying elastance, has been shown to be conserved during the isovolumic contraction period in humans (28).

(b) If we consider the onset of ejection (end of isovolumic contraction) for E_d, then E_es(sb) can be computed from (a) using E_Nd, stroke volume (which equals V_d-V_es), diastolic arterial pressure (which equals P_d) and systolic arterial pressure (which equals P_es/0.9), as follows: E_es(sb) = [P_d-(E_Nd × P_s × 0.9)]/[SV × E_Nd] (27).

(c) To estimate E_Nd, a regression model has been developed using systolic function (LVEF) and arterial load (ratio of arterial diastolic to systolic pressure), along with pre-ejection and ejection times, as follows: non-invasive estimated normalized left ventricular elastance at the onset of ejection [E_Nd(est)] = 0.0275–0.165 × LVEF + 0.3656 × (P_d/P_s) + 0.515 × E_Nd(avg); where E_Nd(avg) (group-average normalized left ventricular elastance at the onset of ejection) = Σa_i × t_Nd^i; a_i values are 0.35695, –7.2266, 74.249, –307.39, 684.54. –856.92, 571.95, and –159.1 for i = 0 to i = 7, respectively; the t_Nd value is determined by the ratio of pre-ejection time (R-wave to flow onset) to total ejection time (R-wave to end-flow), with the time at onset defined by the aortic Doppler waveform (29).

Global function

We also assessed the heart-arterial interaction with the ventricular-arterial coupling (VAC) = $\frac{Ea}{Ees(sb)}$ (30), as suggested by recent guidelines (31); a VAC > 1.36 was considered as reflecting uncoupling with an alteration of LV ejection efficiency (32, 33). LV stroke volume, cardiac output and cardiac index were derived from LVOT diameter and VTI. We also assessed cardiac power index (CPI), a parameter of global cardiac function representing the cardiac pumping ability which is an important determinant of outcome in cardiogenic shock = $\frac{meanarterialpressure{\left(mmHg\right)}^{*}cardiacindex(L.mi{n}^{-1}.m^{-2})}{145}$ (34, 35). Last, an afterload-adjusted LV ejection fraction (LVEF_EA) was assessed using a simple non-linear approach as recently proposed = $LVejectionfractio{n}^{*}\sqrt{\text{arterial}}\mathrm{elastance}$ (36).

Other variables collected

The following data were collected at inclusion: age, sex, body mass index, past medical history, standard treatments, Sequential Organ Failure Assessment (SOFA) score (37), Simplified Acute Physiologic Score (SAPS) II (38). In addition, we collected septic shock characteristics, hemodynamic parameters (blood pressure and heart rate before and after steroid administration, fluid balance, dose of norepinephrine, inotrope need, arterial blood lactate, cardiac rhythm, ventilatory support with respiratory mechanics (mode of ventilation, tidal volume, respiratory rate, positive end expiratory pressure, plateau pressure, driving pressure, respiratory system compliance) for patients under invasive ventilation at the time of the echo examinations. Moreover, the following outcomes were recorded: renal replacement therapy, presence of acute respiratory distress syndrome (ARDS) as per the Berlin definition and vital status at ICU discharge.

Definitions

We defined septic cardiomyopathy as the appearance at echocardiography of hypokinesia (LVEF < 45% or when inotrope infusion was needed to achieve a value ≥ 45%) during the first day of septic shock with the absence of acute coronary syndrome or history of chronic heart failure in the medical charts. We assessed the change in MAP between baseline and 2–4 h after steroid administration. We defined steroid MAP responders (non-responders) as patients with a relative increase in MAP above (below) the median value of the cohort.

Statistical analysis

Statistical analyses were performed with the JMP software (version 14; SAS Institute Inc., Cary, NC) and Graph-Pad Prism software (version 8; GraphPad Software Inc., La Jolla, CA, USA). The primary endpoint of this study was the change in LV contractility after the first administration of low-dose steroid therapy. For the analysis of the primary endpoint, the LV-GLS was chosen for its robustness, reproducibility and sensitivity. We calculated that a sample size of at least 43 patients would have a 90% power to detect a 20% improvement in LV-GLS after low-dose steroid therapy initiation, considering a baseline LV-GLS of –14% with a standard deviation (SD) of 4%, based on previous echocardiographic studies in septic shock patients (39–41). Taking into account the feasibility of LV-GLS in our ICU (42), we therefore planned to include a total of 50 patients. In addition, we performed subgroup analyses. We compared the evolution of echocardiographic and hemodynamic parameters in response to low-dose steroids according to the presence on septic cardiomyopathy and according to MAP response. Descriptive statistics are presented as mean (+/–SD), median (interquartile range), or proportion, based on data type and distribution. Normality of continuous variables was assessed with the Shapiro–Wilk test. Comparisons of categorical variables were made using the Chi-2 test or Fisher exact test, as appropriate. Continuous variables were compared using a T-test or Mann-Withney test for independent variables, and a paired T-test or paired Wilcoxon test for paired variables, with Benjamini-Hochberg correction for multiple comparisons. The reproducibility of some echocardiographic variables in our laboratory was assessed using the British Standards Institution coefficient (twice the standard deviation of the differences in repeated measurements) (43). A p-value < 0.05 in bilateral configuration was considered for statistical significance.

Results

Patient characteristics

During the study period, 188 patients admitted for septic shock and receiving steroid therapy were screened for enrolment. Among these patients, 135 were eligible for the study and 85 were excluded for logistical reasons, poor echogenicity, hemodynamic instability, or withdrawing of life support; finally, 50 patients were included in the study (Figure 1). The logistical reasons for excluding eligible patients from this study were lack of echocardiography-trained operators and urgent examination or procedure. Clinical characteristics, comorbidities and organ failures at inclusion are presented in Table 1. Patients were included a median of 12 [6–20] h after septic shock onset. In this cohort, fludrocortisone was administrated in 41 (82%) patients because the enteral access was not available in all patients (contraindication or waiting for the radiographic control of the gastric tube placement). Echocardiographic and hemodynamic data before and after initiation of low-dose steroids are shown in Table 2. The reproducibility of some echocardiographic variables is reported in Supplementary Table 1. The median delay between the two assessments was 172 (135–218) min. Respiratory settings were comparable between the two hemodynamic assessments.

FIGURE 1

Figure 1. Flow-chart.

TABLE 1

Table 1. Clinical characteristics of study patients and organ failures.

TABLE 2

Table 2. Evolution of hemodynamic and echocardiographic parameters in septic shock patients before and after initiation of low-dose steroid therapy.

Hemodynamics

At inclusion, all patients were receiving norepinephrine, at a median dose of 3.8 (2.3–5.5) mg/h, after a significant fluid resuscitation [median fluid balance of 3 (2–4) L since admission] (Table 1). As compared to baseline values, there was a significant increase in systolic, mean and diastolic arterial pressure after low-dose steroids, along with a significant decrease in heart rate (Table 2).

Loading conditions

To the exception of LV end-systolic wall stress, all afterload parameters significantly improved after low-dose steroid therapy (with an increase in end-systolic arterial elastance, systemic vascular resistance, diastolic arterial pressure, and a decrease in diastolic shock index) (Table 2). Among preload parameters, only E/e’ significantly decreased after low-dose steroid therapy. However, this variation might not be considered as clinically relevant. We did not find a significant change in global function (stroke volume, cardiac index, and CPI).

Contractility and ventriculo-arterial coupling

To the exception of LVEF, all contractility parameters (LV global longitudinal strain, tissue Doppler peak systolic wave at mitral annulus, and-systolic maximal elastance by single-beat method) significantly improved after low-dose steroid therapy (Table 2, Figure 2, and Supplementary Figure 1). VAC significantly decreased after low-dose steroid therapy; uncoupled VAC was present in 18/41 (44%) before low-dose steroid therapy and 10/41 (24%) after (p < 0.01).

FIGURE 2

Figure 2. Data bars of median values of percent relative variation in hemodynamic and echocardiographic parameters after initiation of low-dose steroid therapy in septic shock patients. *Denote an adjusted p-value < 0.05 with Benjamini-Hochberg correction as compared to before with after low-dose steroids therapy.

Subgroups analyses

Overall, the hemodynamic and echocardiographic changes induced by low-dose steroids were similar in patients with or without septic myocardial dysfunction (Supplementary Figure 2). MAP responders significantly improved their macrocirculation and afterload parameters, contrary to non-responders (Supplementary Figure 3).

Discussion

The main findings of this echocardiographic study in septic shock patients were that in addition to their role in peripheral vascular tone (increased afterload), low-dose steroids increased the LV contractility and improved the cardiovascular efficiency.

In this cohort of septic shock patients, the hemodynamic effects of low-dose steroids therapy were coherent with the literature. Indeed, all arterial pressures (systolic, diastolic, and mean) increased in accordance with previous reports on this topic (7). Moreover, all LV afterload parameters assessed (except one) improved after low-doses steroid therapy, also in accordance with previous reports (5, 44). These hemodynamic effects were not homogeneous in the entire cohort as reflected by a greater increase in afterload in MAP responders as compared to non-responders. Although septic myocardial dysfunction was previously shown to be associated with relative adrenal insufficiency (45), we found no difference in the cardiovascular effects of low-dose steroids between patients with and without septic myocardial dysfunction.

In previous studies evaluating hemodynamic effects of hydrocortisone in human septic shock, hydrocortisone increased MAP and systemic vascular resistances, with either decreased cardiac index and heart rate (5, 44) or no obvious effects on cardiac performance (4). The decrease in heart rate may be ascribable to fever control (46). However, these studies did not assess cardiac contractility per se. Our finding of increased cardiac contractility with low-dose steroids is in accordance with previous reports suggesting inotropic properties (47) and a possible protective effect on myocardium in the early phase of septic shock (10). Adrenal insufficiency is a cause of cardiac failure (48) and hydrocortisone increased cardiac output in healthy volunteers with hypoaldosteronism (49). The increase in LV contractility induced by low-dose steroids in septic shock was not necessarily expected. Indeed, some LV contractility indices are inversely correlated with afterload during septic shock (9). New echocardiographic tools (tissue Doppler and strain) seem to be more suitable than traditional parameters of systolic function (LVEF) to detect subtle myocardial dysfunction, and to be less influenced by afterload than other LV contractility parameters during sepsis (50). LV strain and especially tissue Doppler s’ were less prone to changes in LV loading conditions (9). Our data showed a significant increase of these parameters of systolic function without any change of LVEF.

End-systolic elastance is a theoretical load independent variable of LV contractile function deduced from the pressure-volume loops, obtained by invasive ventricular catheterization. Herein, we assessed LV end-systolic elastance by the bedside single beat method proposed by Chen et al. (27), which is considered the most accurate non-invasive method and is recommended by guidelines (31). Despite the improvement in afterload with increased end-systolic arterial elastance after initiation of corticosteroid replacement therapy, we detected a significant increase in LV end-systolic elastance by the single-beat method. The reported increase in this variable may be credible since tissue Doppler s’ and strain were also increased. However, Chen’s methods presents the following limitations: (i) it is based on complex assumptions, equations, and regression models; (ii) echo-derived LVEF is incorporated in E_es(sb) estimate; (ii) the formula of E_es(sb) uses the ratio of pre-ejection to total systolic time raised to multiple powers, such that small variations in the measurements of time intervals may lead to relatively large changes in the estimated E_es(sb) value.

VAC reflects the complex interaction between LV performance and arterial load and represents cardiovascular efficiency. When this ratio between arterial elastance and LV elastance at systole is close to 1.0, it indicates an optimal coupling between LV and arterial system resulting in efficient LV stroke work (51). In septic shock, both arterial elastance and LV elastance can profoundly be altered with various degrees of vasoplegia and/or LV myocardial depression. Evaluation of VAC was recently proposed, not only to understand the hemodynamic pathophysiology in septic shock, but also to predict the response to cardiovascular therapies (52). VAC is mathematically related to LVEF. However, this theoretical relationship is correct only if V_o (an extrapolated value representing the left ventricular volume intercept of the volume axis at a theoretical end-systolic pressure of 0 mmHg) is assumed to be null, like in the normal heart, but this is not the case in heart failure or during hemodynamic instability (53). We herein demonstrate a significant improvement in VAC (tending toward 1.0) after initiation of low-dose steroids with decrease of uncoupled VAC. This result suggest a holistic effect of low-dose steroids on the hemodynamic system, not restricted to the vascular system, but also involving the cardiac contractility with an improved interaction between the heart and the vasculature. However, we cannot exclude a homeometric adaption of the heart. Indeed, according to the Anrep’s effect (54), the heart may adapt to increased loading through an increased contractility to preserve ventricular-arterial coupling.

The main strength of our study is the comprehensive hemodynamic assessment involving modern echocardiographic tools. Steroid replacement therapy improves global outcomes in septic shock patients in some studies but not all. Depicting the effect of steroids on cardiac contractility could be interesting to personalize this therapy. Moreover, cardiac and vascular effect of low-dose steroid therapy may justify extending this therapy to patients in cardiogenic shock (55). Our study also has some limitations. First, the design was monocentric and unblinded, with a relatively limited sample size. Second, the delay between the two echocardiographic evaluations was not constant (time window between 2 and 4 h), due to logistical issues and the pragmatic design. Although this time window is debatable, it was chosen in reference to the previous physiological studies on hemodynamic impact of hydrocortisone and fludrocortisone (49, 56). Third, we did not to assess the hemodynamic effect of low-dose steroids based on adrenal function, and future studies are needed to scrutinize this point. Fourth, excluded patients with unstable hemodynamics may have different cardiac contractile properties and vascular system responses to corticosteroids. Fifth, we could not perform a multivariable because of the limited sample size and the collinearity between some hemodynamic parameters. Sixth, although catecholamine dose and fluid management were kept constant between the two-ultrasound assessments, we cannot exclude their influence on observed hemodynamic modifications. For example, fluids and vasopressors both have inotropic effects via Starling and Anrep effect, respectively, among other mechanisms.

Finally, the interpretation of the hemodynamic variations could be limited by the absence of a control group and the open label design, inasmuch as the hemodynamic profile of septic shock may vary with time. Indeed, while the delay between the two-ultrasound assessments was relatively short, the relationship between low-dose steroid therapy and modification of hemodynamics could be influence by the natural course of septic shock.

Conclusion

We herein report a comprehensive assessment by bedside echocardiography of the hemodynamic impact of low-dose steroids in the early phase of septic shock. We confirmed an increase of arterial pressure, a decrease of heart rate and an improvement of LV afterload after initiation of low-dose steroids. Moreover, we suggested for the first time an increase of LV contractility with improvement of the cardiovascular efficiency as assessed by VAC. However, this possible positive inotropic effect needs to be confirmed in future studies.

Data availability statement

The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.

Ethics statement

The study was approved on July 12, 2018 by the Institutional Ethics Committee, Institutional Review Board (IRB) Mondor (reference number: 2201948) as a component of standard care, and patient consent was waived. Written and oral information about the study was given to the families.

Author contributions

FB had full access to all of the data in the study and took responsibility for the integrity of the data, and the accuracy of the data analysis. FB and AM designed the study and wrote the manuscript. FB, AC, PM, and KR performed echocardiographies. FB, AC, and PM collected the data. FB, AC, PM, NP, GC, KR, and AM contributed to interpretation of data, drafting of the submitted article, and critical revisions for intellectual content. All authors read and approved the final manuscript.

Acknowledgments

We are very indebted to all physicians and nurses of the Henri Mondor Medical Intensive Care Unit for their help for the care of patients.

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Supplementary material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcvm.2022.948231/full#supplementary-material

Supplementary Figure 1 | Box and whiskers plot of percent relative variation in hemodynamic and echocardiographic parameters after initiation of low-dose steroid therapy in septic shock patients. SAP, systolic arterial pressure; PP, pulse pressure; MAP, mean arterial pressure; HR, heart rate; LVEDV, left ventricle end-diastolic volume; E/A, ratio of early to late pulsed-wave Doppler of diastolic transmitral flow velocity; E/e’, ratio of early pulsed-wave Doppler to early tissue Doppler diastolic wave velocity at the mitral valve annulus; e’, early tissue Doppler diastolic wave velocity at the mitral valve annulus; VTI LVOT, velocity-time integral of left ventricular outflow tract; Ea, end-systolic arterial elastance; SVR, systemic vascular resistance; LV-ESWS, left ventricular end-systolic wall stress; DAP, diastolic arterial pressure; DSI, diastolic shock index; LVEF, left ventricular ejection fraction; LVEF_EA, afterload-adjusted LVEF (LVEF x √Ea); LV-GLS, left ventricular global longitudinal strain; S’, tissue Doppler peak systolic wave at mitral annulus; Ees(sb), left ventricular end-systolic maximal elastance by single-beat method; CO, cardiac output; CI, cardiac index; CPI, cardiac power index; VAC, ventricular arterial coupling.

Supplementary Figure 2 | Data bars of median values of percent relative variation in hemodynamic and echocardiographic parameters after initiation of low-dose steroid therapy in septic shock patients, according septic cardiomyopathy. *Denote an adjusted p-value < 0.05 with Benjamini-Hochberg correction as compared to septic cardiomyopathy and non-septic cardiomyopathy.

Supplementary Figure 3 | Data bars of median values of percent relative variation in hemodynamic and echocrdiographic parameters after initiation of low-dose steroid therapy in septic shock patients, according to steroid responders. *Denote an adjusted p-value < 0.05 with Benjamini-Hochberg correction as compared to responders and non-responders.

Abbreviations

MAP, mean arterial pressure; LV, left ventricle; ICU, intensive care unit; ACCP, American College of Chest Physicians; SCCM, Society of Critical Care Medicine; LVEF, left ventricular ejection fraction; IRB, institutional review board; TTE, left transthoracic echocardiography; PRICES, Preferred Reporting Items for Critical care Echocardiography Studies; E, early wave of transmitral diastolic blood flow; A, late wave of transmitral diastolic blood flow; e’, tissue Doppler diastolic wave velocity at mitral valve annulus; VTI, velocity time integral; LVOT, left ventricle outflow tract; DAP, diastolic arterial pressure; E_a, end-systolic arterial elastance; DSI, diastolic shock index; s’, tissue Doppler imaging, with peak systolic wave; LV-GLS, left ventricle global longitudinal peak systolic strain; E_es, left ventricle end-systolic maximal elastance; E_es(sb), E_es estimated by the modified single-beat method; E_d, left ventricular end-diastolic elastance; P_d, diastolic arterial pressure; V_d, left ventricular volume at the onset of ejection; V₀, volume axis intercept of the end-systolic pressure volume relation; V_es, left ventricular end-systolic volume; SV, stroke volume; [E_nd(est)], non-invasive estimated normalized left ventricular elastance at the onset of ejection; [E_nd(avg)], group-average normalized left ventricular elastance at the onset of ejection; VAC, ventricular-arterial coupling; CPI, cardiac power index; LVEF_EA, LVEF adjusted by E_a; SOFA, Sequential Organ Failure Assessment; SAPS, Simplified Acute Physiologic Score; ARDS, of acute respiratory distress syndrome; SD, standard deviation.

References

1. Annane D, Pastores SM, Arlt W, Balk RA, Beishuizen A, Briegel J, et al. Critical illness-related corticosteroid insufficiency (CIRCI): a narrative review from a multispecialty task force of the society of critical care medicine (SCCM) and the European society of intensive care medicine (ESICM). Intensive Care Med. (2017) 43:1781–92. doi: 10.1007/s00134-017-4914-x

CrossRef Full Text | Google Scholar

2. Rhen T, Cidlowski JA. Antiinflammatory action of glucocorticoids–new mechanisms for old drugs. N Engl J Med. (2005) 353:1711–23.

Google Scholar

3. Yard AC, Kadowitz PJ. Studies on the mechanism of hydrocortisone potentiation of vasoconstrictor responses to epinephrine in the anesthetized animal. Eur J Pharmacol. (1972) 20:1–9. doi: 10.1016/0014-2999(72)90209-9

PubMed Abstract | CrossRef Full Text | Google Scholar

4. Bollaert PE, Charpentier C, Levy B, Debouverie M, Audibert G, Larcan A. Reversal of late septic shock with supraphysiologic doses of hydrocortisone. Crit Care Med. (1998) 26:645–50. doi: 10.1097/00003246-199804000-00010

PubMed Abstract | CrossRef Full Text | Google Scholar

5. Briegel J, Forst H, Haller M, Schelling G, Kilger E, Kuprat G, et al. Stress doses of hydrocortisone reverse hyperdynamic septic shock: a prospective, randomized, double-blind, single-center study. Crit Care Med. (1999) 27:723–32.

PubMed Abstract | Google Scholar

6. Oppert M, Reinicke A, Graf KJ, Barckow D, Frei U, Eckardt KU. Plasma cortisol levels before and during “low-dose” hydrocortisone therapy and their relationship to hemodynamic improvement in patients with septic shock. Intensive Care Med. (2000) 26:1747–55.

Google Scholar

7. Bellissant E, Annane D. Effect of hydrocortisone on phenylephrine–mean arterial pressure dose-response relationship in septic shock. Clin Pharmacol Ther. (2000) 68:293–303. doi: 10.1067/mcp.2000.109354

CrossRef Full Text | Google Scholar

8. Annane D, Sebille V, Charpentier C, Bollaert PE, Francois B, Korach JM, et al. Effect of treatment with low doses of hydrocortisone and fludrocortisone on mortality in patients with septic shock. JAMA. (2002) 288:862–71.

Google Scholar

9. Boissier F, Razazi K, Seemann A, Bedet A, Thille AW, de Prost N, et al. Left ventricular systolic dysfunction during septic shock: the role of loading conditions. Intensive Care Med. (2017) 43:633–42. doi: 10.1007/s00134-017-4698-z

CrossRef Full Text | Google Scholar

10. Liao R, Zhang J, Zhao H, Zhang Z, Yang K. [Protective effect of low-dose hydrocortisone on myocardium in early septic shock]. Zhonghua Wei Zhong Bing Ji Jiu Yi Xue. (2020) 32:210–4. doi: 10.3760/cma.j.cn121430-20191024-00039

CrossRef Full Text | Google Scholar

11. Chauvet J-L, El-Dash S, Delastre O, Bouffandeau B, Jusserand D, Michot J-B, et al. Early dynamic left intraventricular obstruction is associated with hypovolemia and high mortality in septic shock patients. Crit Care. (2015) 19:262. doi: 10.1186/s13054-015-0980-z

CrossRef Full Text | Google Scholar

12. Dellinger RP, Levy MM, Rhodes A, Annane D, Gerlach H, Opal SM, et al. Surviving Sepsis Campaign: international guidelines for management of severe sepsis and septic shock, 2012. Intensive Care Med. (2013) 39:165–228.

Google Scholar

13. Annane D, Pastores SM, Rochwerg B, Arlt W, Balk RA, Beishuizen A, et al. Guidelines for the diagnosis and management of critical illness-related corticosteroid insufficiency (CIRCI) in critically ill patients (Part I): society of critical care medicine (SCCM) and European society of intensive care medicine (ESICM) 2017. Intensive Care Med. (2017) 43:1751–63. doi: 10.1007/s00134-017-4919-5

CrossRef Full Text | Google Scholar

14. Rhodes A, Evans LE, Alhazzani W, Levy MM, Antonelli M, Ferrer R, et al. Surviving Sepsis Campaign: international guidelines for management of sepsis and septic shock: 2016. Intensive Care Med. (2017) 43:304–77. doi: 10.1007/s00134-017-4683-6

CrossRef Full Text | Google Scholar

15. Expert Round Table on Echocardiography in ICU. International consensus statement on training standards for advanced critical care echocardiography. Intensive Care Med. (2014) 40:654–66. doi: 10.1007/s00134-014-3228-5

CrossRef Full Text | Google Scholar

16. Sanfilippo F, Huang S, Herpain A, Balik M, Chew MS, Clau-Terré F, et al. The PRICES statement: an ESICM expert consensus on methodology for conducting and reporting critical care echocardiography research studies. Intensive Care Med. (2021) 47:1–13. doi: 10.1007/s00134-020-06262-5

CrossRef Full Text | Google Scholar

17. Nagueh SF, Smiseth OA, Appleton CP, Byrd BF, Dokainish H, Edvardsen T, et al. Recommendations for the evaluation of left ventricular diastolic function by echocardiography: an update from the American society of echocardiography and the European association of cardiovascular imaging. J Am Soc Echocardiogr. (2016) 29:277–314. doi: 10.1016/j.echo.2016.01.011

CrossRef Full Text | Google Scholar

18. Slama M, Masson H, Teboul J-L, Arnout M-L, Susic D, Frohlich E, et al. Respiratory variations of aortic VTI: a new index of hypovolemia and fluid responsiveness. Am J Physiol Heart Circ Physiol. (2002) 283:H1729–33.

Google Scholar

19. Vignon P, Repessé X, Bégot E, Léger J, Jacob C, Bouferrache K, et al. Comparison of echocardiographic indices used to predict fluid responsiveness in ventilated patients. Am J Respir Crit Care Med. (2017) 195:1022–32. doi: 10.1164/rccm.201604-0844OC

CrossRef Full Text | Google Scholar

20. Chirinos JA, Segers P. Noninvasive evaluation of left ventricular afterload: part 2: arterial pressure-flow and pressure-volume relations in humans. Hypertension. (2010) 56:563–70. doi: 10.1161/HYPERTENSIONAHA.110.157339

CrossRef Full Text | Google Scholar

21. Greim CA, Roewer N, Schulte am Esch J. Assessment of changes in left ventricular wall stress from the end-systolic pressure-area product. Br J Anaesth. (1995) 75:583–7. doi: 10.1093/bja/75.5.583

PubMed Abstract | CrossRef Full Text | Google Scholar

22. Kelly RP, Ting CT, Yang TM, Liu CP, Maughan WL, Chang MS, et al. Effective arterial elastance as index of arterial vascular load in humans. Circulation. (1992) 86:513–21. doi: 10.1161/01.CIR.86.2.513

CrossRef Full Text | Google Scholar

23. Ospina-Tascón GA, Teboul J-L, Hernandez G, Alvarez I, Sánchez-Ortiz AI, Calderón-Tapia LE, et al. Diastolic shock index and clinical outcomes in patients with septic shock. Ann Intensive Care. (2020) 10:41.

Google Scholar

24. Lang RM, Bierig M, Devereux RB, Flachskampf FA, Foster E, Pellikka PA, et al. Recommendations for chamber quantification. Eur J Echocardiogr. (2006) 7:79–108. doi: 10.1016/j.euje.2005.12.014

CrossRef Full Text | Google Scholar

25. Gudmundsson P, Rydberg E, Winter R, Willenheimer R. Visually estimated left ventricular ejection fraction by echocardiography is closely correlated with formal quantitative methods. Int J Cardiol. (2005) 101:209–12. doi: 10.1016/j.ijcard.2004.03.027

CrossRef Full Text | Google Scholar

26. Seo JS, Kim DH, Kim WJ, Song JM, Kang DH, Song JK. Peak systolic velocity of mitral annular longitudinal movement measured by pulsed tissue Doppler imaging as an index of global left ventricular contractility. Am J Physiol Heart Circ Physiol. (2010) 298:H1608–15. doi: 10.1152/ajpheart.01231.2009

CrossRef Full Text | Google Scholar

27. Chen C-H, Fetics B, Nevo E, Rochitte CE, Chiou K-R, Ding P-A, et al. Noninvasive single-beat determination of left ventricular end-systolic elastance in humans. J Am Coll Cardiol. (2001) 38:2028–34. doi: 10.1016/S0735-1097(01)01651-5

CrossRef Full Text | Google Scholar

28. Senzaki H, Chen C-H, Kass DA. Single-beat estimation of end-systolic pressure-volume relation in humans. Circulation. (1996) 94:2497–506. doi: 10.1161/01.CIR.94.10.2497

CrossRef Full Text | Google Scholar

29. Shishido T, Hayashi K, Shigemi K, Sato T, Sugimachi M, Sunagawa K. Single-beat estimation of end-systolic elastance using bilinearly approximated time-varying elastance curve. Circulation. (2000) 102:1983–9. doi: 10.1161/01.cir.102.16.1983

CrossRef Full Text | Google Scholar

30. Sunagawa K, Maughan WL, Burkhoff D, Sagawa K. Left ventricular interaction with arterial load studied in isolated canine ventricle. Am J Physiol. (1983) 245:H773–80.

Google Scholar

31. Ikonomidis I, Aboyans V, Blacher J, Brodmann M, Brutsaert DL, Chirinos JA, et al. The role of ventricular–arterial coupling in cardiac disease and heart failure: assessment, clinical implications and therapeutic interventions. A consensus document of the European society of cardiology working group on aorta & Peripheral vascular diseases, European association of cardiovascular imaging, and heart failure association. Eur J Heart Fail. (2019) 21:402–24. doi: 10.1002/ejhf.1436

CrossRef Full Text | Google Scholar

32. Chen C-H, Nakayama M, Nevo E, Fetics BJ, Maughan WL, Kass DA. Coupled systolic-ventricular and vascular stiffening with age. J Am Coll Cardiol. (1998) 32:1221–7. doi: 10.1016/S0735-1097(98)00374-X

CrossRef Full Text | Google Scholar

33. Guarracino F, Ferro B, Morelli A, Bertini P, Baldassarri R, Pinsky MR. Ventriculoarterial decoupling in human septic shock. Crit Care Lond Engl. (2014) 18:R80. doi: 10.1186/cc13842

CrossRef Full Text | Google Scholar

34. Yildiz O, Aslan G, Demirozu ZT, Yenigun CD, Yazicioglu N. Evaluation of resting cardiac power output as a prognostic factor in patients with advanced heart failure. Am J Cardiol. (2017) 120:973–9. doi: 10.1016/j.amjcard.2017.06.028

CrossRef Full Text | Google Scholar

35. Fincke R, Hochman JS, Lowe AM, Menon V, Slater JN, Webb JG, et al. Cardiac power is the strongest hemodynamic correlate of mortality in cardiogenic shock: a report from the SHOCK trial registry. J Am Coll Cardiol. (2004) 44:340–8.

Google Scholar

36. Monge García MI, Jian Z, Settels JJ, Hunley C, Cecconi M, Hatib F, et al. Determinants of left ventricular ejection fraction and a novel method to improve its assessment of myocardial contractility. Ann Intensive Care. (2019) 9:48. doi: 10.1186/s13613-019-0526-7

CrossRef Full Text | Google Scholar

37. Vincent JL, Moreno R, Takala J, Willatts S, De Mendonca A, Bruining H, et al. The SOFA (Sepsis-related Organ Failure Assessment) score to describe organ dysfunction/failure. On behalf of the working group on sepsis-related problems of the European society of intensive care medicine. Intensive Care Med. (1996) 22:707–10. doi: 10.1007/BF01709751

PubMed Abstract | CrossRef Full Text | Google Scholar

38. Le Gall JR, Lemeshow S, Saulnier F. A new simplified acute physiology score (SAPS II) based on a European/North American multicenter study. JAMA. (1993) 270:2957–63.

Google Scholar

39. Orde SR, Pulido JN, Masaki M, Gillespie S, Spoon JN, Kane GC, et al. Outcome prediction in sepsis: speckle tracking echocardiography based assessment of myocardial function. Crit Care. (2014) 18:R149. doi: 10.1186/cc13987

CrossRef Full Text | Google Scholar

40. Shahul S, Gulati G, Hacker MR, Mahmood F, Canelli R, Nizamuddin J, et al. Detection of myocardial dysfunction in septic shock: a speckle-tracking echocardiography study. Anesth Analg. (2015) 121:1547–54. doi: 10.1213/ANE.0000000000000943

CrossRef Full Text | Google Scholar

41. Ng PY, Sin WC, Ng AK-Y, Chan WM. Speckle tracking echocardiography in patients with septic shock: a case control study (SPECKSS). Crit Care Lond Engl. (2016) 20:145. doi: 10.1186/s13054-016-1327-0

CrossRef Full Text | Google Scholar

42. Bagate F, Masi P, d’Humières T, Al-Assaad L, Chakra LA, Razazi K, et al. Advanced echocardiographic phenotyping of critically ill patients with coronavirus-19 sepsis: a prospective cohort study. J Intensive Care. (2021) 9:12. doi: 10.1186/s40560-020-00516-6

CrossRef Full Text | Google Scholar

43. Bland JM, Altman DG. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet Lond Engl. (1986) 1:307–10.

Google Scholar

44. Keh D, Boehnke T, Weber-Cartens S, Schulz C, Ahlers O, Bercker S, et al. Immunologic and hemodynamic effects of “low-dose” hydrocortisone in septic shock: a double-blind, randomized, placebo-controlled, crossover study. Am J Respir Crit Care Med. (2003) 167:512–20. doi: 10.1164/rccm.200205-446OC

CrossRef Full Text | Google Scholar

45. Bagate F, Razazi K, Boissier F, Seemann A, de Prost N, Carteaux G, et al. Association between relative adrenal insufficiency and septic cardiomyopathy: a preliminary report. Intensive Care Med. (2017) 43:1924–6. doi: 10.1007/s00134-017-4901-2

CrossRef Full Text | Google Scholar

46. Schortgen F, Charles-Nelson A, Bouadma L, Bizouard G, Brochard L, Katsahian S. Respective impact of lowering body temperature and heart rate on mortality in septic shock: mediation analysis of a randomized trial. Intensive Care Med. (2015) 41:1800–8. doi: 10.1007/s00134-015-3987-7

CrossRef Full Text | Google Scholar

47. Tanz RD, Kerby CF. The inotropic action of certain steroids upon isolated cardiac tissue; with comments on steroidal cardiotonic structure-activity relationships. J Pharmacol Exp Ther. (1961) 131:56–64.

PubMed Abstract | Google Scholar

48. Mekontso-Dessap A, Marrache D, Vieillard-Baron A. Images in cardiology: acute adrenal insufficiency complicated by cardiogenic shock. Heart. (2005) 91:e31. doi: 10.1136/hrt.2004.055095

CrossRef Full Text | Google Scholar

49. Laviolle B, Le Maguet P, Verdier MC, Massart C, Donal E, Laine F, et al. Biological and hemodynamic effects of low doses of fludrocortisone and hydrocortisone, alone or in combination, in healthy volunteers with hypoaldosteronism. Clin Pharmacol Ther. (2010) 88:183–90. doi: 10.1038/clpt.2010.83

CrossRef Full Text | Google Scholar

50. Hestenes SM, Halvorsen PS, Skulstad H, Remme EW, Espinoza A, Hyler S, et al. Advantages of strain echocardiography in assessment of myocardial function in severe sepsis: an experimental study*. Crit Care Med. (2014) 42:e432–40. doi: 10.1097/CCM.0000000000000310

CrossRef Full Text | Google Scholar

51. De Tombe PP, Jones S, Burkhoff D, Hunter WC, Kass DA. Ventricular stroke work and efficiency both remain nearly optimal despite altered vascular loading. Am J Physiol Heart Circ Physiol. (1993) 264:H1817–24. doi: 10.1152/ajpheart.1993.264.6.H1817

CrossRef Full Text | Google Scholar

52. Pinsky MR, Guarracino F. How to assess ventriculoarterial coupling in sepsis. Curr Opin Crit Care. (2020) 26:313–8. doi: 10.1097/MCC.0000000000000721

CrossRef Full Text | Google Scholar

53. Mihaileanu S, Antohi E. Revisiting the relationship between left ventricular ejection fraction and ventricular–arterial coupling. ESC Heart Fail. (2020) 7:2214–22. doi: 10.1002/ehf2.12880

CrossRef Full Text | Google Scholar

54. von Anrep G. On the part played by the suprarenals in the normal vascular reactions of the body. J Physiol. (1912) 45:307–17. doi: 10.1113/jphysiol.1912.sp001553

CrossRef Full Text | Google Scholar

55. Mekontso Dessap A, Bagate F, Delmas C, Morichau-Beauchant T, Cholley B, Cariou A, et al. Low-dose corticosteroid therapy for cardiogenic shock in adults (COCCA): study protocol for a randomized controlled trial. Trials. (2022) 23:4. doi: 10.1186/s13063-021-05947-6

CrossRef Full Text | Google Scholar

56. Laviolle B, Donal E, Le Maguet P, Laine F, Bellissant E. Low doses of fludrocortisone and hydrocortisone, alone or in combination, on vascular responsiveness to phenylephrine in healthy volunteers. Br J Clin Pharmacol. (2013) 75:423–30. doi: 10.1111/j.1365-2125.2012.04359.x

CrossRef Full Text | Google Scholar