Nicholas M. Shaw1
Jose L. Rios-Monterrosa1
Gregory R. Fedorchak2Margaret R. Ketterer1Gary S. Coombs3
Jan Lammerding2
Lori L. Wallrath1*- 1Department of Biochemistry, Carver College of Medicine, University of Iowa, Iowa City, IA, United States
- 2The Nancy E. and Peter C. Meinig School of Biomedical Engineering, Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, NY, United States
- 3Biology Department, Waldorf University, Forest City, IA, United States
The nuclei of multinucleated skeletal muscles experience substantial external force during development and muscle contraction. Protection from such forces is partly provided by lamins, intermediate filaments that form a scaffold lining the inner nuclear membrane. Lamins play a myriad of roles, including maintenance of nuclear shape and stability, mediation of nuclear mechanoresponses, and nucleo-cytoskeletal coupling. Herein, we investigate how disease-causing mutant lamins alter myonuclear properties in response to mechanical force. This was accomplished via a novel application of a micropipette harpooning assay applied to larval body wall muscles of Drosophila models of lamin-associated muscular dystrophy. The assay enables the measurement of both nuclear deformability and intracellular force transmission between the cytoskeleton and nuclear interior in intact muscle fibers. Our studies revealed that specific mutant lamins increase nuclear deformability while other mutant lamins cause nucleo-cytoskeletal coupling defects, which were associated with loss of microtubular nuclear caging. We found that microtubule caging of the nucleus depended on Msp300, a KASH domain protein that is a component of the linker of nucleoskeleton and cytoskeleton (LINC) complex. Taken together, these findings identified residues in lamins required for connecting the nucleus to the cytoskeleton and suggest that not all muscle disease-causing mutant lamins produce similar defects in subcellular mechanics.
Introduction
The nuclei of skeletal muscles experience high levels of mechanical force during muscle development and contraction of muscle fibers during work (Rosen & Baylies, 2017; Sweeney & Hammers, 2018). Muscle cells possess structural components to withstand such force, including high expression levels of A-type lamins, lamins A and C (Swift et al., 2013; Cho et al., 2017). Lamins, intermediate filaments that line the inner nuclear membrane, provide structural support for the nucleus, determine the nuclear shape, and organize genomic DNA for proper gene expression (Goldman et al., 2004; Burke & Stewart, 2006; Swift et al., 2013; Zwerger et al., 2013; Camozzi et al., 2014; Dialynas et al., 2015; Kim et al., 2017; Wong et al., 2022). Dominant mutations in the LMNA gene encoding the A-type lamins, A and C, cause Emery-Dreifuss muscular dystrophy, limb-girdle muscular dystrophy type 1B, and congenital muscular dystrophy (Bonne et al., 1999; Rankin & Ellard, 2006; Astejada et al., 2007; Worman & Bonne, 2007; Lu et al., 2011; Maggi et al., 2016). Lamins have a conserved domain structure consisting of a head domain, a central coiled-coil rod domain, and a tail domain possessing an Ig-like fold (Dhe-Paganon et al., 2002; Krimm et al., 2002; Mounkes et al., 2003; Herrmann et al., 2007). Lamins dimerize through their rod domain, form filaments through head-to-tail interactions and antiparallel lateral assembly, through lateral contacts that are not well understood to form a meshwork underlying the inner nuclear membrane (Kapinos et al., 2010; Dittmer & Misteli, 2011; Turgay & Medalia, 2017; Eldirany et al., 2021).
The mechanisms by which mutations in the LMNA gene cause muscular dystrophy remain incompletely understood and are a topic of intense investigation. Studies in cultured cells and model organisms of muscular dystrophy-associated LMNA mutations have revealed insights into potential pathomechanisms (Stewart et al., 2007; Zwerger et al., 2013; Rzepecki & Gruenbaum, 2018; Earle et al., 2020; Coombs et al., 2021). These studies have shown that mutant lamins cause nuclear chromatin protrusions, transient nuclear envelope rupture, increased DNA damage, and abnormal intracellular signaling (Goldman et al., 2004; Dialynas et al., 2015; Hatch & Hetzer, 2016; Earle et al., 2020; Coombs et al., 2021). A potential mechanism to explain these defects is that LMNA mutations impair nuclear stability and disrupt nucleo-cytoskeletal connections, which may be particularly devastating in mechanically active tissues such as skeletal and cardiac muscles (Folker et al., 2011; Zwerger et al., 2013; Shin & Worman, 2022).
Cytoskeletal forces are transmitted to the nucleus through the linker of nucleoskeleton and cytoskeleton (LINC) complex (Crisp et al., 2006; Wang et al., 2009; Cain et al., 2018; Hieda, 2019). The LINC complex is comprised of nesprins, proteins that span the outer nuclear membrane and possess a Klarsicht-ANC1-Syne-homology (KASH) domain (Sosa et al., 2012; Wang et al., 2012; Horn, 2014; Kim et al., 2015), and Sad1 and UNC-84 (SUN) domain proteins that span the inner nuclear membrane (Crisp et al., 2006; Wang et al., 2009; Cain et al., 2018; Hieda, 2019). Mammals have six genes encoding a variety of KASH domain proteins, with further complexity generated by alternative splicing (Zhang et al., 2001; Potter & Hodzic, 2018). Mutations in SYNE1 and SYNE2, encoding nesprin 1 and 2, respectively, cause Emery-Dreifuss muscular dystrophy (Zhang et al., 2007; Heller et al., 2020). The nesprin KASH domain connects to the SUN domain proteins across the nuclear lumen. Mammals have two genes encoding SUN proteins, SUN1 and SUN2 (Dreger et al., 2001; Hodzic et al., 2004). SUN domain proteins interact with lamins, thereby linking the nucleoskeleton to the cytoskeleton. DNA sequence variation in SUN1 and SUN2 might modify muscular dystrophy severity (Meinke et al., 2014).
The goal of this study was to determine the effects of specific mutant lamins on the nuclear shape and nucleo-cytoskeletal coupling in muscle. To achieve this goal, we generated Drosophila models of LMNA muscular dystrophy possessing either wild-type or mutant Lamin C (LamC) transgenes. The LamC transgenic lines have similar genetic backgrounds, with the exception of the site of insertion of the P-element. LamC is an orthologue of human LMNA and the only A-type lamin encoded by the Drosophila genome. The Drosophila LamC protein shares a domain structure that includes 35% amino acid identity and 54% similarity with human lamin A/C. Like human LMNA, the expression of endogenous LamC is initiated upon cellular differentiation (Riemer et al., 1995). The transcriptional regulators that drive muscle cell differentiation are similar between Drosophila and humans (Tapscott et al., 1988; Maire et al., 2020). In both species, differentiated myoblasts fuse to form multinucleated muscle fibers that attach to tendon cells (Ovalle, 1987; Krimm et al., 2002; Schulman et al., 2015; Lemke & Schnorrer, 2018; Richier et al., 2018; Balakrishnan et al., 2021). Thus, much of muscle development and physiology is shared between the two species. Herein, we use the Drosophila larval body wall muscles as a proxy for human skeletal muscles. Larval body wall muscles are represented by over 300 individual muscle fibers that can easily be prepared as a muscle fillet (Ramachandran & Budnik, 2010). The use of larval body wall muscles allows for whole organism muscle function assays, cytological analyses, and fillets for in situ microharpooning assays to measure nuclear-cytoskeletal coupling and nuclear deformability.
Muscle-specific expression of mutant lamins in an otherwise wild-type Drosophila background caused dominant effects on muscle physiology and/or function. These Drosophila models of lamin-associated muscle disease revealed vast differences among the mutant lamins tested. Specific mutant lamins altered nuclear shape and succumbed to nuclear envelope deformation under force application. By contrast, other mutant lamins partially uncoupled the nucleus from the cytoskeleton. Interestingly, the domain of lamin affected did not correlate with the loss of a particular nuclear defect, suggesting a complex structure/function relationship, potentially involving additional binding partners. Muscles expressing mutant lamins that caused uncoupling of the nucleus from the cytoskeleton showed a lack of microtubule nuclear caging. A lack of microtubule caging was also observed upon RNAi knockdown of Msp300, a Drosophila KASH domain protein. Taken together, these data suggest that specific residues within lamin alter nucleo-cytoskeletal coupling that is supported by Msp300. Furthermore, mutation-specific cellular defects suggest that different pathological mechanisms might lead to the common muscle atrophy associated with LMNA skeletal muscle disease.
Materials and methods
Drosophila stocks
Drosophila stocks were cultured in cornmeal/sucrose media at 25°C (Shaffer et al., 1994). To generate the Lamin C (LamC) transgenic lines, full length LamC (Gold Clone, accession number AY095046, Berkeley Drosophila Genome Project, available from the Drosophila Genomics Resource Center, Bloomington, IN) was cloned into the pUAST P-element transformation vector (Brand & Perrimon, 1993). This vector contains a minimal promoter downstream of five upstream activating sequences (UAS) that bind the yeast Gal4 transcriptional activator. Standard embryo injection procedures were used to generate the transgenic stocks in which the P-element was relatively randomly inserted within the genome (BestGene, Chino Hills). Eight to ten independent transgenic lines were established for each LamC construct. The site of insertion was mapped to a specific chromosome, and the transgenes were made homozygous. Insertions that were not homozygous viable were discarded. Western analysis was performed, and transgenes that expressed levels of LamC relative to that of the endogenous LamC gene were used for analysis. Muscle-specific expression was achieved by crossing the transgenic lines to the C57 Gal4 driver stock that expresses the yeast Gal4 specifically in larval body wall muscles (Brand and Perrimon, 1993; Gorczyca et al., 2007). The resulting progeny express either wild-type or mutant LamC in their larval body wall muscles.
Viability assays
Genetic crosses were performed in plastic vials with standard medium, cultured at 25°C. Parental flies were removed after larvae were observed in the vials. Dead pupae and live flies were counted for approximately one and a half weeks after the parental adults were removed. Percent viability was calculated by dividing the number of live flies by the total number of live flies plus dead pupae. The total number of living adults plus dead pupae counted for each genotype ranged from 43 to 555. An ANOVA analysis was performed to determine statistical significance. Error bars shown represent 95% confidence interval analyses in GraphPad Prism using the Wilson/Brown method.
Microharpoon assay and analysis
Microharpoons were generated from borosilicate glass rods (Sutter; OD: 1.0 mm, ID: 0.78, 10 cm length) using a Sutter P-97 micropipette puller. The following parameters were used to achieve tip diameters of ≈1 μm and a suitable taper length: HEAT = 500; PULL = 250; VEL = 220; TIME = 200. Drosophila third instar larvae were dissected to expose their body wall muscles. Muscle fillets were secured with magnets on a custom-built, microscope-compatible dissection apparatus and submerged in muscle dissection buffer (128 mM NaCl; 5 mM Hepes, pH 7.4; 2 mM KCl; 35 mM sucrose) supplemented with the fluorescent DNA-binding dye Hoechst 33342 (2 μg/ml) to allow visualization of myonuclei.
The microharpoon assay was performed as previously described (Fedorchak & Lammerding, 2016; Lombardi et al., 2011b), with slight modifications to the pull parameters to accommodate the technique’s use on semi-intact muscle preparations. The microharpoon was inserted into the cytoskeleton ∼10–15 μm from the edge of the nucleus (based on crosshairs in the objective) and pulled 30 μm in the direction away from the nucleus at a rate of 2 μm/s using custom MATLAB software to control the motorized micromanipulator (Eppendorf InjectMan NI2). The pull direction was along the long axis of the myofiber and away from the nucleus. Images were acquired at ×32 magnification (×20 objective with ×1.6 Optivar) every five seconds.
Nucleo-cytoskeletal connectivity was assessed using a custom MATLAB program (MATLAB 2010, Natick, MA) available upon request. For each nucleus, the user first selected a binary threshold value from a histogram of “pixel count versus intensity” to account for heterogeneity in the Hoechst 33342 signal, which the program thresholds to provide an accurate trace of the nucleus during deformation. After applying erosion and dilation processing steps to smooth the thresholded nucleus, the program uses the MATLAB “regionprops” function to track the centroid of the nucleus, generate a nuclear bounding box, and extract the necessary parameters to fit an ellipse to the nucleus. Changes in nuclear strain, centroid displacement, and additional parameters were computed. For most calculations, the frame prior to micropipette harpoon removal (frame of maximum pull) was compared with the first frame before the initiation of the pull. Myonuclei were excluded from analysis if 1) the myofiber was at an angle > ±30° from the vertical axis, 2) the micropipette harpoon was not inserted at the proper distance (≈10–15 µm) from the nuclear envelope, 3) the microharpoon failed to enter the cytoplasm and instead brushed over the top of the fiber, and 4) the myofiber retracted during or after the micropipette harpoon pull.
Immunohistochemistry
Third instar larval body wall muscle dissections were performed according to published procedures (Budnik et al., 1990). After fixation in 4% paraformaldehyde, preparations were stored in 1X PBS. Muscle fillets were washed three times in 1X PBS for five minutes each wash. Muscle fillets were then washed three times in permeabilization buffer (1X PBS, 0.5% Triton-X-100, and 5 mM MgCl2) for five minutes each wash. Preparations were stained with Texas Red®-X Phalloidin (1:400 dilution) in permeabilization buffer containing 0.5% boiled/filtered fish skin gelatin (G-7765, Sigma-Aldrich, St. Louis) and stained with either guinea pig anti-Msp300 antibodies (kind gift from T. Volk; 1:100 dilution), mouse anti-Klar-C (#9C10, Developmental Studies Hybridoma Bank University of Iowa, Iowa City 1:25 dilution), rat anti-Koi (kind gift from J.A. Fischer; either 1:20 or 1:50 dilution), mouse anti-α-tubulin (#12G10, Developmental Studies Hybridoma Bank University of Iowa, Iowa City; 1:200 dilution), and mouse anti-lamin C (1:200 dilution) or mouse anti-lamin Dm0 (#ADL84.12; Developmental Studies Hybridoma Bank University of Iowa, Iowa City; 1:400 dilution). Nuclear pores were stained with MAb414 (ab24609, Abcam, Cambridge, United Kingdom; mouse monoclonal antibody, 1:2,000 dilution). Microscopy was performed using either a Leica DMLB phase contrast fluorescence microscope and a Zeiss 710 confocal microscope or a Leica Thunder fluorescence microscope. Three channel images were merged to produce composite images using ImageJ.
Quantification of the cytoplasmic and nuclear immunofluorescent signal was performed using the integration intensity feature of Fiji (Schindelin et al., 2012). Briefly, nuclei were selected as regions of interest (ROI) using the cell counting function. Muscle fibers were visualized by phalloidin staining and outlined using the trace tool. Nuclear staining was quantified using the integrative intensity feature. A cytoplasmic signal was quantified by subtracting the integrative intensity of the nuclei from the total signal intensity. The correction factor was calculated by drawing a circle in an area lacking muscle and measuring the integrated intensity. This measurement was divided by the area of the circle to yield the correction factor. The background signal was determined by multiplying the area of the ROI by the correction factor, then subtracting this value from the raw integrated intensity values.
Quantification of perinuclear microtubular staining was performed visually by three individuals blinded to the genotype of the larvae. The final number of nuclei assigned to each phenotype represents the average of the three measurements. Each phenotype is represented as a percent of the total nucleus score.
Larval motility assays
Quantitative measurements of larval velocity (mm/min) were made for the host stock (w1118) and larvae expressing either wild-type or mutant LamC in larval body wall muscles via the C57 Gal4 driver (Gorczyca, 2007; Brand, 1993). Motility was also quantified for larvae with body wall muscle-specific expression of either luciferase RNAi or an RNAi transgene against a LINC complex component. For motility assays, third instar larvae were placed on a room temperature 1.8% agarose-filled 15-cm Petri dish for ten minutes to allow adjustment to a new substrate. Following, five larvae were placed in the center of a second 1.8% agarose-filled Petri dish marked with concentric circles. Their crawling was videotaped for 2 minutes using a cell phone. Also, the videos were analyzed using wrMTack, a plug-in for ImageJ (Brooks et al., 2016). A calibration line was drawn from the first concentric circle to the second using the “line tool” to produce the calibration length value. The path crawled by each larva was traced by generating a Z-project using the max intensity function in ImageJ. Distances traveled were measured by tracing larval paths with the segmented line drawing tool. Distances were divided by the calibration length value, resulting in the distance in millimeters. Velocities were calculated by dividing the distance traveled by the recording time (120 s). Two groups of five larvae were measured per genotype. The resulting values were plotted using GraphPad Prism (GraphPad Prism version 8.0.0 for Mac, GraphPad Software, San Diego, California, United States), and velocities were compared to those of the wild type using a one-way ANOVA with Holm-Sidak’s correction.
Statistics
Groups of datasets were analyzed using a one-way analysis of variance (ANOVA). Comparisons between two datasets that showed a normal distribution were analyzed using the Student’s t-test. Excel (16051.14931.20132.0, Microsoft, Seattle) and GraphPad Prism (v.8.0.0 for Mac, GraphPad Software, San Diego) were used to generate graphs and perform statistical analyses. Error bars in graphs represent means ± standard deviation (SD) unless otherwise noted. All results reported were derived from a minimum of three independent biological samples.
Results
Drosophila larval body wall muscle-specific expression of mutant lamins caused premature death
We modeled eight LMNA mutations in Drosophila LamC that were selected based on their causality in human disease (Figure 1A; Table 1). These mutations altered amino acids in all three lamin protein domains. Some of the amino acid changes affected amino acids that are identical between human lamin A/C and Drosophila LamC (S37L, K47, L74, R205, and R237), while others are represented by conservative substitutions (R237 and K521).
FIGURE 1. Muscle-specific expression of mutant LamC causes premature death. (A) Diagram of the Drosophila LamC domain structure with the head (red), rod (blue), and tail (green) domains indicated. The positions of the amino acid changes examined in this study are shown with the corresponding human amino acid changes in parentheses. (B) Three-dimensional model of full-length human lamin A (Hinz et al., 2021) with the position of the amino acid changes in the head and rod domain indicated by arrows. The positions of amino acid changes in the Ig fold are indicated in the magnified view (panel C). (C) Enlarged views of the three-dimensional lamin A/C Ig-fold domain based on an NMR structure (PBD 1IVT) (Krimm et al., 2002) with the amino acid substitutions examined here labeled. (D) Larvae with body wall muscle-specific expression of wild-type and mutant LamC were allowed to develop and scored for adult viability. Percent viability was calculated by dividing the number of live adults by the total number of offspring (adult flies plus dead pupae). The X-axis shows the genotype, and the Y-axis shows the percent viability. Larvae expressing LamC S37L, ΔK47, R205W, R237P, and K521Q yielded no surviving adults. Larvae expressing LamC L74R, G489V, and R564P had a significant reduction in survivability to adulthood. Colors correspond to the protein domain where the amino acid change is located: head (red), rod (blue), and tail (green). Fisher’s exact test was used to compare values for the mutants versus the wild-type control. Total progeny scored per genotype ranged from 43 to 555. Error bars represent 95% confidence intervals. ****, p ≤ 0.0001. Bio-icons in panel D were created with BioRender.com.
TABLE 1. Lamin A/C amino acid changes examined in this study.
The amino acid changes under investigation were mapped onto the three-dimensional structure of lamin A/C that is based on a combination of experimental data and in silico predictions (Figure 1B) (Krimm et al., 2002; Strelkov et al., 2004; Lilina et al., 2020; Hinz et al., 2021). The amino acid residues S22 and K32 map within a short-predicted alpha-helical region of the head domain (Figure 1B). The amino acid residues L59, R190, and H22 map within the long alpha-helical regions of the rod domain (“coiled coil”). The amino acid residue G449V maps to a loop region within the Ig fold (Figure 1C). By contrast, amino acid residues R482 and R527 map to β-sheets in the Ig fold (Figure 1C). Thus, by testing this set of amino acid changes, we are surveying all three conserved lamin domains.
To determine the effects of these mutant lamins on the physical properties of myonuclei, we expressed the mutant lamins in Drosophila larval body wall muscles using the tissue-specific Gal4/UAS system (Caygill & Brand, 2016). The C57 Gal4 driver stock expresses the Gal4 transcription factor in larval body wall muscles throughout larval development (Brand & Perrimon, 1993; Gorczyca et al., 2007). These flies were crossed with flies expressing either wild-type or mutant LamC transgenes. Larvae with body wall muscle-specific expression of wild-type LamC developed normally; they crawled up the vial wall, underwent metamorphosis to pupae, and emerged as adults with no apparent defects. By contrast, larvae with body wall-specific expression of mutant LamC exhibited difficulties crawling up the vial wall and died during the late pupal stage. Death at this stage is consistent with loss of larval body wall muscle function, which is required for morphogenesis (Fortier et al., 2003; Dialynas et al., 2010). To quantify the effects of the mutant lamins on viability, the fraction of viable adults was calculated by dividing the number of live adult flies by the number of total progeny (live plus dead pupae). A small percentage of viable adults resulted from the expression of LamC L74R, G489V, and R564P (Figure 1D). However, no adult progeny resulted upon expression of LamC S37L, ΔK47, R205W, R237P, and K521Q (Figure 1D). Western analysis of protein extracted from larval carcasses (mostly body wall muscle) revealed that the lethality was caused by the mutant LamC, not by general over-expression of LamC. LamC levels were similar in all genotypes, except for R564P, which had high variable expression among biological replicates (Supplementary Figures S1A, B). Thus, amino acid substitutions in all three lamin domains cause lethality at the pupal stage.
Specific mutant lamins cause nuclear envelope protein mislocalization
To investigate the cause of lethality due to mutant versions of LamC at the cellular level, immunohistochemistry was performed on larval body wall muscles. Muscles expressing either wild-type or mutant LamC transgenes were stained with antibodies against LamC, lamin Dm0 (the only B-type lamin in Drosophila), and FG-repeat containing nuclear pore proteins. Antibody staining showed that wild-type LamC localization was confined to the nucleus, particularly the nuclear envelope, as observed for the host stock w1118 (Figure 2; Supplementary Figure S2). Myonuclei expressing LamC R205W were abnormally shaped, with LamC confined to the nucleus; however, nuclear aggregates were also apparent (Figure 2; Supplementary Figure S2). By contrast, myonuclei expressing LamC S37L, L74R, G489V, K521Q, and R564P had a spherical nuclear shape and showed cytoplasmic aggregation of LamC. Thus, the mutant lamins exhibited a range of abnormal cellular localization patterns that did not correlate with the domain of lamin affected.
FIGURE 2. Muscle-specific expression of mutant LamC causes cytoplasmic lamin aggregation. (A) Third instar larval body wall muscles expressing either wild-type or mutant LamC were dissected and stained with antibodies to LamC (green), phalloidin (magenta), and DAPI (blue). Representative images for an amino acid substitution in each of the three LamC domains are shown. Images of muscle expressing all of the amino acid changes examined here are shown in Supplementary Figure S2. Scale bar: 30 μm. (B) Percentage of nuclear LamC was quantified by measuring the intensity of nuclear LamC antibody staining divided by the total amount of staining in the muscle cell (i.e., nuclear plus cytoplasmic LamC) and multiplying by 100. Three to six muscle fibers containing five to 29 nuclei from two to three larvae were analyzed. Each data point represents the average percent nuclear signal in a muscle fiber. The mean and standard deviation of the values obtained for muscle fibers of each genotype are shown. A one-way ANOVA analysis was used to determine statistical significance. *, p ≤ 0.05; **, p ≤ 0.01; ***, ≤0.001; ****, p ≤ 0.0001.
In humans, A- and B-type lamins form independent networks underlying the inner nuclear envelope (Shimi et al., 2015). To determine if the mutant LamC proteins perturbed the distribution of the Drosophila B-type lamin, lamin Dm0, larval body wall muscles expressing either wild-type or mutant LamC transgenes were stained with antibodies specific to lamin Dm0. Neither expression of wild-type LamC nor mutant LamC perturbed the localization of lamin Dm0 at the nuclear envelope (Supplementary Figure S3). These data strongly suggest that the two lamin types form independent networks in Drosophila, like in humans, and that mutant LamC does not overtly interfere with lamin Dm0 organization.
Lamins interact with many different proteins in the nucleus, including those that make up nuclear pores (NUPs) (Wilson & Foisner, 2010; Kittisopikul et al., 2021). To determine if the mutant lamins altered the localization of nuclear pore proteins, larval body wall muscles expressing either wild-type or mutant LamC transgenes were stained with antibodies to FG-repeat-containing NUPs. As anticipated, muscles expressing wild-type LamC showed punctate anti-NUP staining at the nuclear envelope (Figure 3; Supplementary Figure S4). In contrast, in muscles expressing LamC ΔK47, the anti-NUP staining was distributed unevenly around the nuclear envelope (Supplementary Figure S4). Perhaps this reflects the involvement of lamins in the proper spacing of nuclear pores (Furukawa et al., 2009; Kittisopikul et al., 2021). Intriguingly, in muscles expressing LamC R205W, R237P, G489V, K521Q, and R564P, the NUPs mislocalized to the cytoplasm (Figure 3; Supplementary Figure S4). Mislocalization may cause depletion of the NUPs in the envelope, affecting nuclear–cytoplasmic transport. In addition, cytoplasmic aggregation of NUPs could be toxic to cytoplasmic events such as intracellular signaling and maintenance of redox homeostasis (Rajasekaran et al., 2007; Chivet et al., 2020; Bobylev et al., 2021; Coombs et al., 2021; Potulska-Chromik et al., 2021). Thus, the mutant lamins had differing effects on NUP localization with several residues in the rod and Ig-like fold domains implicated in proper NUP formation. Substitution at these residues caused NUP cytoplasmic aggregation, suggesting the formation of an abnormal A-type lamin meshwork does not support proper assembly or maintenance of the NUPs.
FIGURE 3. Mutant LamC alters the localization of FG-containing nuclear pore proteins. (A) Third instar larval body wall muscles expressing wild-type or mutant LamC were dissected and stained with antibodies to FG-repeat-containing nuclear pore proteins (NUPs) (green), phalloidin (magenta), and DAPI (blue). Representative images for an amino acid substitution in each of the three LamC domains are shown. Results from all amino acid changes studied in this manuscript are shown in Supplementary Figure S4. Scale bar: 30 μm. (B) Percent of nuclear NUP staining was quantified by measuring the intensity of the nuclear FG-repeat antibody staining divided by the total amount of staining (nuclear plus cytoplasmic), then multiplying by 100. Three to six muscle fibers containing three to 28 nuclei from two to three larvae were analyzed. Each data point represents the average percent nuclear signal in a muscle fiber. The mean and standard deviation of the values obtained for muscle fibers of each genotype are shown. A one-way ANOVA analysis was used to determine statistical significance. *, p ≤ 0.05; **, p ≤ 0.01; ***, ≤0.001; ****, p ≤ 0.0001.
Specific mutant lamins cause nucleo-cytoskeletal uncoupling and nuclear deformation
To understand the functional consequences of the mutant lamins on the mechanical properties of myonuclei, we employed a novel application of a microharpooning assay. This assay allows for quantitative assessment of nucleo-cytoskeletal coupling and nuclear deformation under local force application to the perinuclear cytoskeleton (Maniotis et al., 1997; Lombardi et al., 2011a; Fedorchak & Lammerding, 2016). The assay uses a fine-tipped glass needle to “harpoon” the cytoskeleton at a defined position near the nucleus and then applies a precisely defined displacement while monitoring induced nuclear displacement and deformation via real-time fluorescence microscopy imaging. The application described here was the first use of this technique on living, multinucleated muscle fibers in situ. For standard preparation of the larval body wall muscle, a longitudinal cut is made at the ventral midline of the larvae, organs are removed, and the remaining tissue, termed a muscle fillet, represents body wall muscles, attached to tendon cells adhered to the hypodermis (Carayon et al., 2020).
For our studies, larval body wall muscles were dissected, immobilized on a glass slide (Figure 4A), and harpooned in a physiological buffer while mounted on a microscope. A fine-tipped glass needle attached to a computer-controlled micromanipulator was inserted 10–15 μm from the edge of a nucleus and pulled 30 μm at a rate of 2 μm/s. The direction of pull was along the long axis of the muscle fiber and away from the nucleus. Images were captured by time-lapse microscopy for five seconds (Figure 4B; Supplementary Videos S1–S4). From the recorded videos, two types of subcellular mechanical measurements were taken. First, the distance the center of the nucleus was displaced from its original position upon force application by the microharpoon (“nuclear centroid displacement”) served as a quantitative measurement of nucleo-cytoskeletal coupling (Figure 4C). Defects in nucleo-cytoskeletal coupling are expected to result in reduced nuclear centroid displacement (Lombardi et al., 2011b). Second, the extent of nuclear elongation upon force application, normalized to the initial length of the nucleus (“nuclear strain”), served as a quantitative measurement of nuclear deformability (Figure 4D). Relative to the controls, only muscles expressing LamC ΔK47 and K521Q showed a significant decrease in nuclear centroid displacement (Figure 4C), suggesting that these mutant lamins partially uncoupled the nucleus from the cytoskeleton. Compared to controls, only LamC ΔN, L74R, and R205W showed significantly increased nuclear deformation (Figure 4D), suggesting reduced nuclear mechanical stability. We recognize that nuclear deformation in the microharpoon assay can also be influenced by the transmitted force, so an increase in nuclear deformation could also result from increased force transmission to the nucleus, but the more likely explanation appears to be an increase in nuclear deformability. Surprisingly, some mutant lamins (S37L, R237P, G489V, and R564P) had no apparent effect on either nuclear-cytoskeletal coupling or nuclear deformability (Figure 4D). Such findings suggest that these mutants might cause defects unrelated to the mechanical properties of the nucleus such as genome organization. Collectively, these data show that mutant lamins have distinct effects on the physical properties of the nucleus regardless of the lamin domain affected.
FIGURE 4. Specific mutant lamins reduce nucleoskeletal-cytoskeletal coupling and increase nuclear deformation. Body wall muscles from larvae expressing either wild-type or mutant LamC were dissected and maintained in a physiological buffer. (A) Representative semi-intact larval body wall muscle fillet is shown. Such muscle fillets were used for micropipette harpooning assays to measure physical coupling between the nucleus and cytoplasm and nuclear strain. (B) Representative time-lapse images from a video of microharpooning wild-type muscles. Images shown were taken at 0.4, 1.4, and 2.4 s from the video recording. (C) Inset diagram shows the experimental approach with the black arrowhead representing a micropipette, the black arrow indicating the direction of force application, and the yellow arrow representing the movement of the center point of the nucleus, centroid displacement, which is a change in position in the y-direction. Expression of wild-type LamC did not alter centroid displacement compared to that of the Gal4 driver control. In contrast, centroid displacement was reduced for larvae expressing LamC ΔK47 and K521Q, indicating a loss of nucleoskeleton-cytoskeleton coupling. Colors correspond to the domains in which each amino acid change is located: head (red), rod (blue), and tail (green). An ANOVA analysis was used to determine statistical significance among the genotypes. Boxed amino acids are those with statistical differences; 15–48 nuclei were analyzed per genotype. *, p ≤ 0.05; **; p ≤ 0.01. (D) Inset diagram shows the experimental approach with the black arrowhead representing a micropipette needle, the black arrow indicating the direction of force application, and the yellow double-headed arrow representing the measured change in nuclear deformation. Nuclear strain is measured as the length of the nucleus at the end of the force application minus the length of the nucleus upon initial force application divided by the initial length of the nucleus. Expression of wild-type LamC did not alter nuclear strain compared to that of the Gal4 driver-only control. In contrast, muscles expressing LamC ΔN, L74R, and R205W showed increased nuclear strain compared to the controls. An ANOVA analysis was used to determine statistical significance. Boxed amino acids are those with statistical differences; 15–48 nuclei were analyzed per genotype. *, p ≤ 0.05; **, p ≤ 0.01.
Mutant lamins that reduce nucleo-cytoskeletal coupling exhibit loss of myonuclear microtubule caging
Given the lack of an apparent correlation between nuclear mechanical defects and nuclear envelope protein localization, we examined perinuclear cytoskeletal organization as a potential factor. Based on the muscle staining of phalloidin, which binds actin (Supplementary Figures S2–S4), and anti-alpha-actinin, which crosslinks actin (Supplementary Figure S5), no overt muscle-wide defects in actin organization were apparent in the muscles expressing mutant LamC at the light microscope level; however, they could be present. Other typical components of the cytoskeleton include cytoplasmic intermediate filaments and microtubules. However, the Drosophila genome does not contain genes encoding the standard cytoplasmic intermediate filaments such as desmin and vimentin (Herrmann & Strelkov, 2011; Cho et al., 2016). Therefore, we stained with antibodies to α-tubulin, which revealed striking differences among the mutant lamins in perinuclear microtubule organization. In larval body wall muscles expressing wild-type LamC, microtubules form a cage around the nucleus like that observed for the host stock (Figure 5). Similar microtubule cages have also been observed in mammalian cells (Bruusgaard et al., 2006; Earle et al., 2020). The α-tubulin staining pattern of muscles expressing LamC S37L, L74R, R205W, R237P, G489V, and R564P gave similar results to that of the wild type (Figure 5). By contrast, α-tubulin staining of muscles expressing LamC ΔK47 and K521Q showed a loss of microtubule caging around the nucleus. Instead, the microtubules were aligned orthogonal to the long axis of the muscle fiber and did not show any enrichment at the nucleus (Figure 5). Interestingly, the lack of myonuclear microtubule caging was only observed for the two mutants that eliminated nucleo-cytoskeletal coupling and not in the other mutants that had normal nucleo-cytoskeletal force transmission. This suggests that microtubules are critical components for the nucleo-cytoskeletal coupling in Drosophila larval body wall muscles. This idea is supported by the fact that the mutant versions of LamC that cause loss of nuclear microtubule caging also exhibit larval motility defects (Supplementary Figure S6).
FIGURE 5. Specific mutant lamins cause loss of microtubular caging around the nucleus. Third instar larval body wall muscles expressing either wild-type or mutant LamC were stained with antibodies to α-tubulin (green and white in magnified images), phalloidin (magenta), and DAPI (blue). In wild-type muscle, α-tubulin forms a cage around the nucleus as observed for the host stock w1118 and muscles expressing wild-type LamC. Expression of the majority of mutant lamins did not alter the α-tubulin nuclear cage. However, the cage was not apparent in muscles expressing mutant LamC ΔK47 and K521Q. In these cases, the microtubules were arrayed parallel to the length of the muscle fiber (open yellow arrows). Yellow boxes indicate the area magnified in the right panels. Scale Bar: 30 μm.
RNAi knockdown of the LINC complex component Msp300 recapitulates the loss of nuclear microtubule caging
Nesprins are components of the LINC complex and have been shown in some cases to interact with microtubules (Chang et al., 2015; Starr, 2017; Maurer & Lammerding, 2019). The Drosophila LINC complex consists of a single SUN domain protein Klaroid (Koi), encoding the SUN domain protein, and either Msp300 or Klarischt (Klar) as the KASH domain proteins (Yu et al., 2006; Kracklauer et al., 2007; Technau & Roth, 2008; Xie & Fischer, 2008). To assay for the involvement of the LINC complex in perinuclear microtubule organization in Drosophila larval body wall muscles, we used RNAi transgenes to deplete each of the LINC complex components separately in larval body wall muscles. Effective depletion of each protein was confirmed by immunofluorescence, with RNAi targeting luciferase as a negative control (Supplementary Figure S7). Larval body wall muscles typically show nuclear microtubule caging (Figure 5). In muscles expressing the luciferase RNAi control, more than 90% of the myonuclei had the expected microtubule caging (Figures 6A, B). By contrast, larval body wall muscles with depletion of LINC complex components showed a range of myonuclear phenotypes (Figure 6B). Some myonuclei displayed an asymmetrical arrangement of microtubules, with enrichment of microtubules on one side of the nucleus. Other myonuclei completely lacked microtubule caging. Msp300 depletion produced the greatest number of myonuclei that lacked microtubule caging compared to the other genotypes. In the Msp300 depleted cells, the microtubules were aligned orthogonal to the long axis of the muscle fiber, remarkably like the microtubule arrangement in muscles expressing LamC ΔK47 and K521Q (Figure 5). These findings suggest that Msp300 is a crucial link between lamins and perinuclear microtubules.
FIGURE 6. RNAi against LINC complex components disrupts perinuclear microtubule organization. (A) Immunohistochemistry was performed on third instar larval body wall muscles expressing either RNAi against Luciferase (control) or LINC complex components using antibodies to α-tubulin (green), phalloidin (magenta), and DAPI (blue). Open yellow arrows indicate microtubules that run parallel with the long axis of the muscle fiber, giving rise to uncaged nuclei. (B) Representative patterns of microtubule organization around the nucleus are shown at the top. Scale Bar: 10 mm. The graph represents the percentage of nuclei showing each pattern of localization per genotype. Muscles expressing an RNAi against Luciferase (luc) show nearly 100% caged nuclei. In contrast, RNAi knockdown of each of the LINC complex members shows increased numbers of asymmetric and uncaged nuclei. A range of 32–79 nuclei were scored per genotype. Scale Bar: 30 μm.
Given that depletion of Msp300 caused loss of microtubule caging, we hypothesized that this would lead to an uncoupling of the nucleus from the cytoskeleton like that observed in the LamC ΔK47 and K521Q. To test this hypothesis, we performed microharpooning on muscle fibers expressing either luciferase, Klar, or Msp300 RNAi transgenes. The centroid displacement values obtained for muscles depleted for Klar were similar to those of the luciferase RNAi control (Figure 7A; Supplementary Videos S5–S7) and consistent with the normal nuclear microtubule caging observed in the Klar depleted muscle. By contrast, depletion of Msp300 caused a significant reduction in centroid displacement relative to that of the control, indicative of a partial loss of nucleo-cytoskeletal coupling (Figure 7A). Motility assays showed that depletion of Msp300 caused reduced larval motility, suggesting a functional significance for coupling between the nucleoskeleton and the cytoskeleton (Supplementary Figure S8). The Msp300 RNAi transgene is predicted to reduce levels of all known Msp300 isoforms. Given recent findings on isoform-specific functions of Msp300, it is possible that loss of muscle function is due to altered Z disc structure and/or abnormal nuclear positioning (Rey et al., 2021).
FIGURE 7. Muscle-specific RNAi against Msp300 causes loss of nucleoskeletal-cytoskeletal coupling. The micropipette harpooning assay was performed on larval body wall muscles expressing an RNAi transgene against either Luciferase (as a control) or a LINC complex component. (A) Inset diagram shows the experimental approach with the black arrowhead representing a micropipette, the black arrow indicating the direction of force application, and the yellow arrow representing the movement of the center point of the nucleus, centroid displacement, which is a change in position in the Y-direction. RNAi against Msp300 reduced centroid displacement relative to the Luciferase RNAi control, suggesting impaired force transmission between the cytoskeleton and nucleus. In contrast, RNAi against Koi produced no change in nuclear centroid displacement relative to the control. A one-way ANOVA was used to determine statistical significance; 16–28 nuclei were analyzed per genotype. **, p ≤ 0.01 (B) Inset diagram shows the experimental approach with the black arrowhead representing a micropipette needle, the black arrow indicating the direction of force application, and the yellow double-headed arrow representing the measured change in nuclear deformation. Nuclear strain is measured as the length of the nucleus at the end of the force application minus the length of the nucleus upon the initial force application divided by the length at the end of the force application. RNAi knockdown of the LINC complex components did not alter nuclear strain, indicating that LINC complex disruption did not change nuclear stiffness. A one-way ANOVA was used to determine statistical significance. No statistically significant changes among genotypes were observed; 16–28 nuclei were analyzed per genotype.
FIGURE 8. Models show microtubular organization in lamin mutant muscles with impaired nuclear-cytoskeletal connections. Diagram of wild-type muscles in which microtubules form a cage around the nucleus (top). The microtubule cage around the nucleus is reduced in larval body wall muscles expressing either LamC ΔK47 or K521Q (bottom). In these cases, the microtubules run parallel with the long axis of the muscle fiber. In addition, these two mutant lamins cause cytoplasmic mislocalization of nuclear pore proteins (red circles). The microtubule cage around the nucleus is also reduced upon RNAi knockdown of Msp300, a KASH-domain LINC complex component, and the microtubules run parallel to the long axis of the muscle fiber. Bio-icons in the model were created with BioRender.com.
Results from the microharpooning assays suggested that Msp300 depletion did not alter nuclear deformation similar to LamC ΔK47 and K521Q, the two mutant lamins that caused uncoupling of the nucleus and cytoskeleton (Figure 7B). Thus, the microharpooning assay functionally distinguished mutants that alter nuclear mechanics from those that do not. Taken together, our experiments indicate that depletion of Msp300 or expression of LamC ΔK47 and K521Q mutants causes impaired nucleo-cytoskeletal force transmission in Drosophila body wall muscles by disrupting the nuclear microtubule cage (Figures 5, 6 and 8), establishing an important role of the perinuclear microtubule network organized by Msp300 in transmitting cytoskeletal forces to the myonuclei.
Discussion
Mutations in LMNA cause a plethora of disease phenotypes including skeletal muscular dystrophy and dilated cardiomyopathy (Bonne et al., 1999; Bonne et al., 2000; Hermida-Prieto et al., 2004; Pethig et al., 2005) (Table 1). Individuals with the same LMNA mutation can exhibit dramatically different disease presentations and have clinically distinct diagnoses (Muchir et al., 2004; Porcu et al., 2021). This variability can even be observed in closely related family members, strongly implying that phenotypic variability is due to modifier genes. Herein, our goal was to examine muscle defects resulting from specific mutant lamins in a defined genetic background that allows for a direct comparison without the complication of genetic background modifiers. To accomplish this goal, we generated Drosophila models of LMNA-associated muscular dystrophy. In these models, the mutant lamins display dominant defects, as in human diseases (Worman, 2012). Our studies revealed that specific amino acid changes alter the mechanical functions of myonuclei (Figure 4). Some mutant lamins dramatically affected nuclear deformation, while others impaired nucleo-cytoskeletal coupling. Furthermore, some mutant lamins mislocalized and altered nuclear pore protein localization, while others did not. Collectively, these data demonstrate that the altered phenotypes do not correlate with the domain of lamin affected and that different cellular defects can lead to common premature death in these Drosophila models.
Among our observations, we noted that five of the eight amino acid changes in lamin resulted in LamC cytoplasmic aggregation (Figure 2). It is interesting to note that mutant lamins that caused minimal cytoplasmic aggregation altered nuclear shape, a property largely determined by the lamin meshwork (Horn, 2014; Maurer & Lammerding, 2019). Cytoplasmic aggregation of proteins can lead to deleterious consequences for muscle function. Consistent with this idea, increasing rates of autophagy, which reduces cytoplasmic aggregates, suppressed muscle defects caused by mutant lamins in multiple model organisms (Park et al., 2009; Choi et al., 2012; Ramos et al., 2012; Liao et al., 2016; Chiarini et al., 2019; Coombs et al., 2021). In fact, in silico studies suggest that lamin aggregation might serve as a predictor of pathogenicity for LMNA variants of uncertain significance (Anderson et al., 2021). Seven of the eight amino acid changes in LamC resulted in cytoplasmic aggregation of FG-repeat NUPs (Figure 3). It is unclear if the NUPs are improperly inserted or ineffectively anchored in the nuclear envelope. Regardless, a reduction in functional NUPs is likely to affect nuclear–cytoplasmic transport of biomolecules, causing a myriad of defects. Consistent with the results presented here, FG-repeat NUPs were observed in the cytoplasm of human muscle biopsy tissue from LMNA skeletal muscular dystrophy patients, demonstrating clinical relevance (Dialynas et al., 2015).
Herein, we were able to directly assay the functional consequences of mutant lamins on nuclear deformability and nucleo-cytoskeletal coupling via a novel application of a microharpooning assay. Three of the mutant lamins (LamC ΔN, L74R, and R205W) showed increased nuclear deformation under force application (Figure 4). In prior studies using a different means of applying nuclear deformation, LamC ΔN was also found to be highly deformable, validating this novel application (Zwerger et al., 2013). Only two mutant lamins (LamC ΔK47 and K521Q) caused a loss of nucleo-cytoskeletal coupling (Figure 4). Surprisingly, these two mutants do not cause overt mislocalization of Koi and Msp300; however, protein–protein interactions could be perturbed by the mutant lamins without gross mislocalization (Supplementary Figure S9). Additionally, these two mutants did not appear to alter nuclear deformation, demonstrating that loss of physical properties needed for coupling is not necessarily required for maintaining nuclear shape upon force application. However, we cannot rule out that these mutations did not increase nuclear deformability, since it is possible that reduced force transmission from the cytoskeleton to the nucleus counteracted this effect.
A particularly noteworthy finding was that nucleo-cytoskeletal uncoupling by specific mutant lamins and depletion of Msp300 were associated with loss of myonuclear microtubule caging (Figures 5, 7 and Figure 8). Our findings are consistent with those of others showing that a network of microtubules around the nucleus in larval body wall muscles is dependent on Msp300 (Volk, 2013). The mechanisms by which microtubules are recruited to the nuclear envelope in differentiated muscle are incompletely understood (Bugnard et al., 2005; Bartolini & Gundersen, 2006; Rogers et al., 2008; Tillery et al., 2018; Becker et al., 2020). In mouse myoblasts undergoing differentiation, centrosomal protein PCM-1 relocalizes to the nuclear envelope by a mechanism that requires Nesprin 1 (Espigat-Georger et al., 2016). Furthermore, the centrosomal protein AKAP450 is required for microtubule nucleation at the nuclear envelope in differentiated myotubes (Gimpel et al., 2017). Consistent with the role of centrosomal proteins in microtubule recruitment, the large scaffold protein AKAP6 links centrosomal protein AKAP9 and nesprin-1α to nucleate microtubules at the nuclear envelope in rat cardiomyocytes (Vergarajauregui et al., 2020). This process is dependent on the induction of muscle-specific isoforms of AKAP6 and nesprin-1α by the transcriptional regulator myogenin (Becker et al., 2020). Similarly, in the Drosophila fat body, a network of microtubules around the nucleus is stabilized by several centrosomal proteins and a spectraplakin (Shot) that localize at the nuclear envelope (Wang et al., 2015; Sun et al., 2019; Zheng et al., 2020). It will be of interest to identify the proteins that nucleate microtubules at the nuclear envelope in Drosophila larval body wall muscles and investigate how specific mutant lamins alter such interactions.
The functional consequences of the impaired nucleo-cytoskeletal coupling in muscles expressing LamC ΔK47 and K521Q are not known. It is possible that the loss of nuclear microtubule caging reduces larval motility because the non-caged longitudinal microtubules interfere with the actin-myosin contractile apparatus. A dense microtubule network led to increased myocyte stiffness that impaired contractility in failing hearts (Bajpai et al., 2018). In this case, pharmacological treatments leading to the detyrosination of microtubules lowered cytoplasmic viscosity and restored cardiac contractile function. It is also possible that loss of the microtubule nuclear caging in the larval body wall muscle increases nuclear envelope damage, resulting in the loss of muscle function. During muscle contraction, myonuclei move in coordination with the sarcoplasm via connections with the cytoskeleton (Lorber et al., 2020). Uncoupling the nucleus from the cytoskeleton can result in asynchronous movements, which generate variable drag forces on myonuclei during muscle contraction (Lorber et al., 2020). Such drag forces might impact normal nuclear mechanosensing and/or lead to nuclear envelope rupture and DNA damage. Consistent with this idea, increased DNA damage has been observed in LMNA disease models and human muscle biopsy tissue (Earle et al., 2020). Activation of DNA damage response pathways might signal to block muscle contractions to prevent further DNA damage. In these instances, the microtubule nuclear cage is predicted to serve as a shock absorber, providing protection from mechanical force during muscle contraction. Paradoxically, uncoupling the nucleus from the cytoskeleton via disruption of the LINC complex suppresses muscle defects in mouse models of laminopathies (Earle et al., 2020; Chai et al., 2021). In such cases, an altered nuclear lamina might cause nuclei to be especially vulnerable to forces applied from the cytoskeleton; uncoupling the nucleus and cytoskeleton proved beneficial, possibly by reducing forces across the nuclear envelope. Future studies are needed to assess the complex relationship between microtubules and myonuclear integrity.
Only five of the eight mutant lamins studied here showed changes in the physical properties or nucleo-cytoskeletal coupling of myonuclei, as measured by the microharpooning assay, yet all eight caused premature death (Figure 1). The mutant lamins that did not alter nuclear mechanics might play a role in gene expression. Genomes are rich with lamin-associated domains (LADs) in which sections of chromosomes are in close opposition with the lamin meshwork (Mohanta et al., 2021; Wong et al., 2021). In fact, in some cell types, LADs represent up to half of the genome (Briand & Collas, 2020; Mohanta et al., 2021; Rullens & Kind, 2021). In general, LADs are relatively gene poor and lack active transcription. Therefore, the mutant lamins studied here might form an abnormal lamin meshwork that alters contacts with the genome, resulting in altered gene expression.
Collectively, our studies highlight the vast heterogeneity in muscle defects caused by mutant lamins while keeping the genetic background constant. As with other studies of lamins, specific cellular defects do not correlate with alterations in specific protein domains of lamin. Therefore, predictions of how LMNA variants of uncertain significance alter lamin function are challenging. Studies such as these will allow for the grouping of mutant lamins that share similar defective properties, which ultimately will guide treatments.
Data availability statement
The original contributions presented in the study are included in the article/Supplementary Material; further inquiries can be directed to the corresponding author.
Author contributions
NS, GF, MK, GC, JL, and LW contributed to the experimental design. NS, GF, MK, GC, and LW performed experiments. Data were analyzed by NS, JR-M, GF, MK, GC, JL, and LW. The manuscript was prepared by NS, JR-M, JL, and LW. The manuscript was reviewed and edited by NS, JR-M, GF, MK, GC, JL, and LW.
Funding
This research was supported by funds from a Burroughs Welcome Fund Collaborative Research Travel Grant (1017502), the Muscular Dystrophy Association (Development Award MDA603238), the National Institutes of Health (NIH, R21AR075193) to LW; NIH R01 (HL082792) to JL, the National Science Foundation (awards #1715606 and URoL 2022048) to JL, the Volkswagen Foundation Life program (award A130142) to JL, a University of Iowa ICRU Fellowship to NS, and the University of Iowa Carver College of Medicine FUTURE Program support of GC. Bio-icons in Figure 1D and Figure 8 were created with BioRender.com.
Acknowledgments
We thank N. P. Mohar for technical assistance. We are grateful for the gift of antibodies from T. Volk and J. A. Fischer.
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors, and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Supplementary material
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcell.2022.934586/full#supplementary-material
References
Anderson, C. L., Langer, E. R., Routes, T. C., McWilliams, S. F., Bereslavskyy, I., Kamp, T. J., et al. (2021). Most myopathic lamin variants aggregate: A functional genomics approach for assessing variants of uncertain significance. NPJ Genom. Med. 6 (1), 103. doi:10.1038/s41525-021-00265-x
Arbustini, E., Pilotto, A., Repetto, A., Grasso, M., Negri, A., Diegoli, M., et al. (2002). Autosomal dominant dilated cardiomyopathy with atrioventricular block: a lamin A/C defect-related disease. J. Am. Coll. Cardiol. 39 (6), 981–990. doi:10.1016/s0735-1097(02)01724-2
Astejada, M. N., Goto, K., Nagano, A., Ura, S., Noguchi, S., Nonaka, I., et al. (2007). Emerinopathy and laminopathy clinical, pathological and molecular features of muscular dystrophy with nuclear envelopathy in Japan. Acta Myol. 26 (3), 159–164.
Bajpai, G., Schneider, C., Wong, N., Bredemeyer, A., Hulsmans, M., Nahrendorf, M., et al. (2018). The human heart contains distinct macrophage subsets with divergent origins and functions. Nat. Med. 24 (8), 1234–1245. doi:10.1038/s41591-018-0059-x
Balakrishnan, M., Sisso, W. J., and Baylies, M. K. (2021). Analyzing muscle structure and function throughout the larval instars in live Drosophila. Star. Protoc. 2 (1), 100291. doi:10.1016/j.xpro.2020.100291
Bartolini, F., and Gundersen, G. G. (2006). Generation of noncentrosomal microtubule arrays. J. Cell Sci. 119 (20), 4155–4163. doi:10.1242/jcs.03227
Becker, R., Leone, M., and Engel, F. B. (2020). Microtubule organization in striated muscle cells. Cells 9 (6), E1395. doi:10.3390/cells9061395
Bobylev, A. G., Fadeev, R. S., Bobyleva, L. G., Kobyakova, M. I., Shlyapnikov, Y. M., Popov, D. V., et al. (2021). Amyloid aggregates of smooth-muscle Titin impair cell adhesion. Int. J. Mol. Sci. 22 (9), 4579. doi:10.3390/ijms22094579
Bonne, G., Di Barletta, M. R., Varnous, S., Becane, H. M., Hammouda, E. H., Merlini, L., et al. (1999). Mutations in the gene encoding lamin A/C cause autosomal dominant Emery-Dreifuss muscular dystrophy. Nat. Genet. 21 (3), 285–288. doi:10.1038/6799
Bonne, G., Mercuri, E., Muchir, A., Urtizberea, A., Becane, H. M., Recan, D., et al. (2000). Clinical and molecular genetic spectrum of autosomal dominant Emery-Dreifuss muscular dystrophy due to mutations of the lamin A/C gene. Ann. Neurol. 48 (2), 170–180. doi:10.1002/1531-8249(200008)48:2<170::aid-ana6>3.0.co;2-j
Brand, A. H., and Perrimon, N. (1993). Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development 118 (2), 401–415. doi:10.1242/dev.118.2.401
Briand, N., and Collas, P. (2020). Lamina-associated domains: Peripheral matters and internal affairs. Genome Biol. 21 (1), 85. doi:10.1186/s13059-020-02003-5
Brooks, D. S., Vishal, K., Kawakami, J., Bouyain, S., and Geisbrecht, E. R. (2016). Optimization of wrMTrck to monitor Drosophila larval locomotor activity. J. Insect Physiol. 93-94, 11–17. doi:10.1016/j.jinsphys.2016.07.007
Brown, C. A., Lanning, R. W., McKinney, K. Q., Salvino, A. R., Cherniske, E., Crowe, C. A., et al. (2001). Novel and recurrent mutations in lamin A/C in patients with Emery-Dreifuss muscular dystrophy. Am. J. Med. Genet. 102 (4), 359–367. doi:10.1002/ajmg.1463
Bruusgaard, J. C., Liestol, K., and Gundersen, K. (2006). Distribution of myonuclei and microtubules in live muscle fibers of young, middle-aged, and old mice. J. Appl. Physiol. 100 (6), 2024–2030. doi:10.1152/japplphysiol.00913.2005
Budnik, V., Zhong, Y., and Wu, C. F. (1990). Morphological plasticity of motor axons in Drosophila mutants with altered excitability. J. Neurosci. 10 (11), 3754–3768.
Bugnard, E., Zaal, K. J., and Ralston, E. (2005). Reorganization of microtubule nucleation during muscle differentiation. Cell Motil. Cytoskelet. 60 (1), 1–13. doi:10.1002/cm.20042
Burke, B., and Stewart, C. L. (2006). The laminopathies: The functional architecture of the nucleus and its contribution to disease. Annu. Rev. Genomics Hum. Genet. 7, 369–405. doi:10.1146/annurev.genom.7.080505.115732
Cain, N. E., Jahed, Z., Schoenhofen, A., Valdez, V. A., Elkin, B., Hao, H., et al. (2018). Conserved SUN-KASH interfaces mediate LINC complex-dependent nuclear movement and positioning. Curr. Biol. 28 (19), 3086–3097. e3084. doi:10.1016/j.cub.2018.08.001
Camozzi, D., Capanni, C., Cenni, V., Mattioli, E., Columbaro, M., Squarzoni, S., et al. (2014). Diverse lamin-dependent mechanisms interact to control chromatin dynamics. Focus on laminopathies. Nucleus 5 (5), 427–440. doi:10.4161/nucl.36289
Carayon, A., Bataille, L., Lebreton, G., Dubois, L., Pelletier, A., Carrier, Y., et al. (2020). Intrinsic control of muscle attachment sites matching. Elife 9, e57547. doi:10.7554/eLife.57547
Caygill, E. E., and Brand, A. H. (2016). The GAL4 system: A versatile system for the manipulation and analysis of gene expression. Methods Mol. Biol. 1478, 33–52. doi:10.1007/978-1-4939-6371-3_2
Chai, R. J., Werner, H., Li, P. Y., Lee, Y. L., Nyein, K. T., Solovei, I., et al. (2021). Disrupting the LINC complex by AAV mediated gene transduction prevents progression of Lamin induced cardiomyopathy. Nat. Commun. 12 (1), 4722. doi:10.1038/s41467-021-24849-4
Chang, W., Worman, H. J., and Gundersen, G. G. (2015). Accessorizing and anchoring the LINC complex for multifunctionality. J. Cell Biol. 208 (1), 11–22. doi:10.1083/jcb.201409047
Chiarini, F., Evangelisti, C., Cenni, V., Fazio, A., Paganelli, F., Martelli, A. M., et al. (2019). The cutting edge: The role of mTOR signaling in laminopathies. Int. J. Mol. Sci. 20 (4), E847. doi:10.3390/ijms20040847
Chivet, M., Marchioretti, C., Pirazzini, M., Piol, D., Scaramuzzino, C., Polanco, M. J., et al. (2020). Polyglutamine-expanded androgen receptor alteration of skeletal muscle homeostasis and myonuclear aggregation are affected by Sex, Age and muscle metabolism. Cells 9 (2), E325. doi:10.3390/cells9020325
Cho, A., Kato, M., Whitwam, T., Kim, J. H., and Montell, D. J. (2016). An atypical tropomyosin in Drosophila with intermediate filament-like properties. Cell Rep. 16 (4), 928–938. doi:10.1016/j.celrep.2016.06.054
Cho, S., Irianto, J., and Discher, D. E. (2017). Mechanosensing by the nucleus: From pathways to scaling relationships. J. Cell Biol. 216 (2), 305–315. doi:10.1083/jcb.201610042
Choi, J. C., Muchir, A., Wu, W., Iwata, S., Homma, S., Morrow, J. P., et al. (2012). Temsirolimus activates autophagy and ameliorates cardiomyopathy caused by lamin A/C gene mutation. Sci. Transl. Med. 4 (144), 144ra102. 144ra102. doi:10.1126/scitranslmed.3003875
Coombs, G. S., Rios-Monterrosa, J. L., Lai, S., Dai, Q., Goll, A. C., Ketterer, M. R., et al. (2021). Modulation of muscle redox and protein aggregation rescues lethality caused by mutant lamins. Redox Biol. 48, 102196. doi:10.1016/j.redox.2021.102196
Crisp, M., Liu, Q., Roux, K., Rattner, J. B., Shanahan, C., Burke, B., et al. (2006). Coupling of the nucleus and cytoplasm: Role of the LINC complex. J. Cell Biol. 172 (1), 41–53. doi:10.1083/jcb.200509124
Dhe-Paganon, S., Werner, E. D., Chi, Y. I., and Shoelson, S. E. (2002). Structure of the globular tail of nuclear lamin. J. Biol. Chem. 277 (20), 17381–17384. doi:10.1074/jbc.C200038200
Dialynas, G., Shrestha, O. K., Ponce, J. M., Zwerger, M., Thiemann, D. A., Young, G. H., et al. (2015). Myopathic lamin mutations cause reductive stress and activate the nrf2/keap-1 pathway. PLoS Genet. 11 (5), e1005231. doi:10.1371/journal.pgen.1005231
Dialynas, G., Speese, S., Budnik, V., Geyer, P. K., and Wallrath, L. L. (2010). The role of Drosophila Lamin C in muscle function and gene expression. Development 137 (18), 3067–3077. doi:10.1242/dev.048231
Dittmer, T. A., and Misteli, T. (2011). The lamin protein family. Genome Biol. 12 (5), 222. doi:10.1186/gb-2011-12-5-222
Dreger, M., Bengtsson, L., Schoneberg, T., Otto, H., and Hucho, F. (2001). Nuclear envelope proteomics: Novel integral membrane proteins of the inner nuclear membrane. Proc. Natl. Acad. Sci. U. S. A. 98 (21), 11943–11948. doi:10.1073/pnas.211201898
D’Amico, A., Haliloglu, G., Richard, P., Talim, B., Maugenre, S., Ferreiro, A., et al. (2005). Two patients with “Dropped head syndrome” due to mutations in LMNA or SEPN1 genes. Neuromuscul. Disord. 15 (8), 521–524. doi:10.1016/j.nmd.2005.03.006
Earle, A. J., Kirby, T. J., Fedorchak, G. R., Isermann, P., Patel, J., Iruvanti, S., et al. (2020). Mutant lamins cause nuclear envelope rupture and DNA damage in skeletal muscle cells. Nat. Mat. 19 (4), 464–473. doi:10.1038/s41563-019-0563-5
Eldirany, S. A., Lomakin, I. B., Ho, M., and Bunick, C. G. (2021). Recent insight into intermediate filament structure. Curr. Opin. Cell Biol. 68, 132–143. doi:10.1016/j.ceb.2020.10.001
Espigat-Georger, A., Dyachuk, V., Chemin, C., Emorine, L., and Merdes, A. (2016). Nuclear alignment in myotubes requires centrosome proteins recruited by nesprin-1. J. Cell Sci. 129 (22), 4227–4237. doi:10.1242/jcs.191767
Fedorchak, G., and Lammerding, J. (2016). Cell microharpooning to study nucleo-cytoskeletal coupling. Methods Mol. Biol. 1411, 241–254. doi:10.1007/978-1-4939-3530-7_16
Folker, E. S., Ostlund, C., Luxton, G. W., Worman, H. J., and Gundersen, G. G. (2011). Lamin A variants that cause striated muscle disease are defective in anchoring transmembrane actin-associated nuclear lines for nuclear movement. Proc. Natl. Acad. Sci. U. S. A. 108 (1), 131–136. doi:10.1073/pnas.1000824108
Fortier, T. M., Vasa, P. P., and Woodard, C. T. (2003). Orphan nuclear receptor betaFTZ-F1 is required for muscle-driven morphogenetic events at the prepupal-pupal transition in Drosophila melanogaster. Dev. Biol. 257 (1), 153–165. doi:10.1016/s0012-1606(03)00036-8
Furukawa, K., Ishida, K., Tsunoyama, T. A., Toda, S., Osoda, S., Horigome, T., et al. (2009). A-type and B-type lamins initiate layer assembly at distinct areas of the nuclear envelope in living cells. Exp. Cell Res. 315 (7), 1181–1189. doi:10.1016/j.yexcr.2008.12.024
Gimpel, P., Lee, Y. L., Sobota, R. M., Calvi, A., Koullourou, V., Patel, R., et al. (2017). Nesprin-1α-Dependent microtubule nucleation from the nuclear envelope via Akap450 is necessary for nuclear positioning in muscle cells. Curr. Biol. 27 (19), 2999–3009. e2999. doi:10.1016/j.cub.2017.08.031
Goldman, R. D., Shumaker, D. K., Erdos, M. R., Eriksson, M., Goldman, A. E., Gordon, L. B., et al. (2004). Accumulation of mutant lamin A causes progressive changes in nuclear architecture in Hutchinson-Gilford progeria syndrome. Proc. Natl. Acad. Sci. U. S. A. 101 (24), 8963–8968. doi:10.1073/pnas.0402943101
Gorczyca, D., Ashley, J., Speese, S., Gherbesi, N., Thomas, U., Gundelfinger, E., et al. (2007). Postsynaptic membrane addition depends on the Discs-Large-interacting t-SNARE Gtaxin. J. Neurosci. 27 (5), 1033–1044. doi:10.1523/JNEUROSCI.3160-06.2007
Hatch, E. M., and Hetzer, M. W. (2016). Nuclear envelope rupture is induced by actin-based nucleus confinement. J. Cell Biol. 215 (1), 27–36. doi:10.1083/jcb.201603053
Heller, S. A., Shih, R., Kalra, R., and Kang, P. B. (2020). Emery-Dreifuss muscular dystrophy. Muscle Nerve 61 (4), 436–448. doi:10.1002/mus.26782
Hermida-Prieto, M., Monserrat, L., Castro-Beiras, A., Laredo, R., Soler, R., Peteiro, J., et al. (2004). Familial dilated cardiomyopathy and isolated left ventricular noncompaction associated with lamin A/C gene mutations. Am. J. Cardiol. 94 (1), 50–54. doi:10.1016/j.amjcard.2004.03.029
Herrmann, H., Bar, H., Kreplak, L., Strelkov, S. V., and Aebi, U. (2007). Intermediate filaments: From cell architecture to nanomechanics. Nat. Rev. Mol. Cell Biol. 8 (7), 562–573. doi:10.1038/nrm2197
Herrmann, H., and Strelkov, S. V. (2011). History and phylogeny of intermediate filaments: Now in insects. BMC Biol. 9, 16. doi:10.1186/1741-7007-9-16
Hieda, M. (2019). Signal transduction across the nuclear envelope: Role of the LINC complex in bidirectional signaling. Cells 8 (2), E124. doi:10.3390/cells8020124
Hinz, B. E., Walker, S. G., Xiong, A., Gogal, R. A., Schnieders, M. J., and Wallrath, L. L. (2021). In silico and in Vivo analysis of amino acid substitutions that cause laminopathies. Int. J. Mol. Sci. 22 (20), 11226. doi:10.3390/ijms222011226
Hodzic, D. M., Yeater, D. B., Bengtsson, L., Otto, H., and Stahl, P. D. (2004). Sun2 is a novel mammalian inner nuclear membrane protein. J. Biol. Chem. 279 (24), 25805–25812. doi:10.1074/jbc.M313157200
Horn, H. F. (2014). LINC complex proteins in development and disease. Curr. Top. Dev. Biol. 109, 287–321. doi:10.1016/b978-0-12-397920-9.00004-4
Kapinos, L. E., Schumacher, J., Mucke, N., Machaidze, G., Burkhard, P., Aebi, U., et al. (2010). Characterization of the head-to-tail overlap complexes formed by human lamin A, B1 and B2 "half-minilamin" dimers. J. Mol. Biol. 396 (3), 719–731. doi:10.1016/j.jmb.2009.12.001
Kim, D. I., Birendra, K. C., and Roux, K. J. (2015). Making the LINC: SUN and KASH protein interactions. Biol. Chem. 396 (4), 295–310. doi:10.1515/hsz-2014-0267
Kim, J. K., Louhghalam, A., Lee, G., Schafer, B. W., Wirtz, D., and Kim, D. H. (2017). Nuclear lamin A/C harnesses the perinuclear apical actin cables to protect nuclear morphology. Nat. Commun. 8 (1), 2123. doi:10.1038/s41467-017-02217-5
Kittisopikul, M., Shimi, T., Tatli, M., Tran, J. R., Zheng, Y., Medalia, O., et al. (2021). Computational analyses reveal spatial relationships between nuclear pore complexes and specific lamins. J. Cell Biol. 220 (4), e202007082. doi:10.1083/jcb.202007082
Kracklauer, M. P., Banks, S. M., Xie, X., Wu, Y., and Fischer, J. A. (2007). Drosophila klaroid encodes a SUN domain protein required for Klarsicht localization to the nuclear envelope and nuclear migration in the eye. Fly. (Austin) 1 (2), 75–85. doi:10.4161/fly.4254
Krimm, I., Ostlund, C., Gilquin, B., Couprie, J., Hossenlopp, P., Mornon, J. P., et al. (2002). The Ig-like structure of the C-terminal domain of lamin A/C, mutated in muscular dystrophies, cardiomyopathy, and partial lipodystrophy. Structure 10 (6), 811–823. doi:10.1016/s0969-2126(02)00777-3
Lemke, S. B., and Schnorrer, F. (2018). In vivo imaging of muscle-tendon morphogenesis in Drosophila pupae. J. Vis. Exp. 132, 57312. doi:10.3791/57312
Liao, C. Y., Anderson, S. S., Chicoine, N. H., Mayfield, J. R., Academia, E. C., Wilson, J. A., et al. (2016). Rapamycin reverses metabolic Deficits in lamin A/C-deficient mice. Cell Rep. 17 (10), 2542–2552. doi:10.1016/j.celrep.2016.10.040
Lilina, A. V., Chernyatina, A. A., Guzenko, D., and Strelkov, S. V. (2020). Lateral A11 type tetramerization in lamins. J. Struct. Biol. 209 (1), 107404. doi:10.1016/j.jsb.2019.10.006
Lombardi, M. L., Jaalouk, D. E., Shanahan, C. M., Burke, B., Roux, K. J., and Lammerding, J. (2011a). The interaction between nesprins and sun proteins at the nuclear envelope is critical for force transmission between the nucleus and cytoskeleton. J. Biol. Chem. 286 (30), 26743–26753. doi:10.1074/jbc.M111.233700
Lombardi, M. L., Zwerger, M., and Lammerding, J. (2011b). Biophysical assays to probe the mechanical properties of the interphase cell nucleus: Substrate strain application and microneedle manipulation. J. Vis. Exp. 55, 3087. doi:10.3791/3087
Lorber, D., Rotkopf, R., and Volk, T. (2020). A minimal constraint device for imaging nuclei in live Drosophila contractile larval muscles reveals novel nuclear mechanical dynamics. Lab. Chip 20 (12), 2100–2112. doi:10.1039/d0lc00214c
Lu, J. T., Muchir, A., Nagy, P. L., and Worman, H. J. (2011). LMNA cardiomyopathy: Cell biology and genetics meet clinical medicine. Dis. Model. Mech. 4 (5), 562–568. doi:10.1242/dmm.006346
Maggi, L., Carboni, N., and Bernasconi, P. (2016). Skeletal muscle laminopathies: A review of clinical and molecular features. Cells 5 (3), E33. doi:10.3390/cells5030033
Maire, P., Dos Santos, M., Madani, R., Sakakibara, I., Viaut, C., and Wurmser, M. (2020). Myogenesis control by SIX transcriptional complexes. Semin. Cell Dev. Biol. 104, 51–64. doi:10.1016/j.semcdb.2020.03.003
Maniotis, A. J., Chen, C. S., and Ingber, D. E. (1997). Demonstration of mechanical connections between integrins, cytoskeletal filaments, and nucleoplasm that stabilize nuclear structure. Proc. Natl. Acad. Sci. U. S. A. 94 (3), 849–854. doi:10.1073/pnas.94.3.849
Maurer, M., and Lammerding, J. (2019). The driving force: Nuclear mechanotransduction in cellular function, fate, and disease. Annu. Rev. Biomed. Eng. 21, 443–468. doi:10.1146/annurev-bioeng-060418-052139
Meinke, P., Mattioli, E., Haque, F., Antoku, S., Columbaro, M., Straatman, K. R., et al. (2014). Muscular dystrophy-associated SUN1 and SUN2 variants disrupt nuclear-cytoskeletal connections and myonuclear organization. PLoS Genet. 10 (9), e1004605. doi:10.1371/journal.pgen.1004605
Mohanta, T. K., Mishra, A. K., and Al-Harrasi, A. (2021). The 3D genome: From structure to function. Int. J. Mol. Sci. 22 (21), 11585. doi:10.3390/ijms222111585
Mounkes, L., Kozlov, S., Burke, B., and Stewart, C. L. (2003). The laminopathies: Nuclear structure meets disease. Curr. Opin. Genet. Dev. 13 (3), 223–230. doi:10.1016/s0959-437x(03)00058-3
Muchir, A., Medioni, J., Laluc, M., Massart, C., Arimura, T., van der Kooi, A. J., et al. (2004). Nuclear envelope alterations in fibroblasts from patients with muscular dystrophy, cardiomyopathy, and partial lipodystrophy carrying lamin A/C gene mutations. Muscle Nerve 30 (4), 444–450. doi:10.1002/mus.20122
Ovalle, W. K. (1987). The human muscle-tendon junction. A morphological study during normal growth and at maturity. Anat. Embryol. 176 (3), 281–294. doi:10.1007/BF00310184
Park, Y. E., Hayashi, Y. K., Bonne, G., Arimura, T., Noguchi, S., Nonaka, I., et al. (2009). Autophagic degradation of nuclear components in mammalian cells. Autophagy 5 (6), 795–804. doi:10.4161/auto.8901
Pethig, K., Genschel, J., Peters, T., Wilhelmi, M., Flemming, P., Lochs, H., et al. (2005). LMNA mutations in cardiac transplant recipients. Cardiology 103 (2), 57–62. doi:10.1159/000082048
Porcu, M., Corda, M., Pasqualucci, D., Binaghi, G., Sanna, N., Matta, G., et al. (2021). A very long-term observation of a family with dilated cardiomyopathy and overlapping phenotype from lamin A/C mutation. J. Cardiovasc. Med. 22 (1), 53–58. doi:10.2459/JCM.0000000000001060
Potter, C., and Hodzic, D. (2018). Analysis of high molecular Weight isoforms of nesprin-1 and nesprin-2 with vertical agarose Gel electrophoresis. Methods Mol. Biol. 1840, 25–33. doi:10.1007/978-1-4939-8691-0_3
Potulska-Chromik, A., Jedrzejowska, M., Gos, M., Rosiak, E., Kierdaszuk, B., Maruszak, A., et al. (2021). Pathogenic mutations and putative phenotype-affecting variants in polish myofibrillar Myopathy patients. J. Clin. Med. 10 (5), 914. doi:10.3390/jcm10050914
Rajasekaran, N. S., Connell, P., Christians, E. S., Yan, L. J., Taylor, R. P., Orosz, A., et al. (2007). Human alpha B-crystallin mutation causes oxido-reductive stress and protein aggregation cardiomyopathy in mice. Cell 130 (3), 427–439. doi:10.1016/j.cell.2007.06.044
Ramachandran, P., and Budnik, V. (2010). Dissection of Drosophila larval body-wall muscles. Cold Spring Harb. Protoc. 2010 (8), pdb.prot5469. pdb prot5469. doi:10.1101/pdb.prot5469
Ramos, F. J., Chen, S. C., Garelick, M. G., Dai, D. F., Liao, C. Y., Schreiber, K. H., et al. (2012). Rapamycin reverses elevated mTORC1 signaling in lamin A/C-deficient mice, rescues cardiac and skeletal muscle function, and extends survival. Sci. Transl. Med. 4 (144), 144ra103. doi:10.1126/scitranslmed.3003802
Rankin, J., and Ellard, S. (2006). The laminopathies: A clinical review. Clin. Genet. 70 (4), 261–274. doi:10.1111/j.1399-0004.2006.00677.x
Rey, A., Schaeffer, L., Durand, B., and Morel, V. (2021). Drosophila nesprin-1 isoforms differentially contribute to muscle function. Cells 10 (11), 3061. doi:10.3390/cells10113061
Richier, B., Inoue, Y., Dobramysl, U., Friedlander, J., Brown, N. H., and Gallop, J. L. (2018). Integrin signaling downregulates filopodia during muscle-tendon attachment. J. Cell Sci. 131 (16), jcs217133. doi:10.1242/jcs.217133
Riemer, D., Stuurman, N., Berrios, M., Hunter, C., Fisher, P. A., and Weber, K. (1995). Expression of Drosophila lamin C is developmentally regulated: Analogies with vertebrate A-type lamins. J. Cell Sci. 108 (10), 3189–3198. doi:10.1242/jcs.108.10.3189
Rogers, G. C., Rusan, N. M., Peifer, M., and Rogers, S. L. (2008). A multicomponent assembly pathway contributes to the formation of acentrosomal microtubule arrays in interphase Drosophila cells. Mol. Biol. Cell 19 (7), 3163–3178. doi:10.1091/mbc.E07-10-1069
Rosen, J. N., and Baylies, M. K. (2017). Myofibrils put the squeeze on nuclei. Nat. Cell Biol. 19 (10), 1148–1150. doi:10.1038/ncb3618
Rullens, P. M. J., and Kind, J. (2021). Attach and stretch: Emerging roles for genome-lamina contacts in shaping the 3D genome. Curr. Opin. Cell Biol. 70, 51–57. doi:10.1016/j.ceb.2020.11.006
Rzepecki, R., and Gruenbaum, Y. (2018). Invertebrate models of lamin diseases. Nucleus 9 (1), 227–234. doi:10.1080/19491034.2018.1454166
Schindelin, J., Arganda-Carreras, I., Frise, E., Kaynig, V., Longair, M., Pietzsch, T., et al. (2012). Fiji: An open-source platform for biological-image analysis. Nat. Methods 9 (7), 676–682. doi:10.1038/nmeth.2019
Schulman, V. K., Dobi, K. C., and Baylies, M. K. (2015). Morphogenesis of the somatic musculature in Drosophila melanogaster. Wiley Interdiscip. Rev. Dev. Biol. 4 (4), 313–334. doi:10.1002/wdev.180
Shaffer, C. D., Wuller, J. M., and Elgin, S. C. (1994). Raising large quantities of Drosophila for biochemical experiments. Methods Cell Biol. 44, 99–108. doi:10.1016/s0091-679x(08)60908-5
Shimi, T., Kittisopikul, M., Tran, J., Goldman, A. E., Adam, S. A., Zheng, Y., et al. (2015). Structural organization of nuclear lamins A, C, B1, and B2 revealed by superresolution microscopy. Mol. Biol. Cell 26 (22), 4075–4086. doi:10.1091/mbc.E15-07-0461
Shin, J. Y., and Worman, H. J. (2022). Molecular Pathology of laminopathies. Annu. Rev. Pathol. 17, 159–180. doi:10.1146/annurev-pathol-042220-034240
Song, K., Dube, M. P., Lim, J., Hwang, I., Lee, I., and Kim, J. J. (2007). Lamin A/C mutations associated with familial and sporadic cases of dilated cardiomyopathy in Koreans. Exp. Mol. Med. 39 (1), 114–120. doi:10.1038/emm.2007.13
Sosa, B. A., Rothballer, A., Kutay, U., and Schwartz, T. U. (2012). LINC complexes form by binding of three KASH peptides to domain interfaces of trimeric SUN proteins. Cell 149 (5), 1035–1047. doi:10.1016/j.cell.2012.03.046
Starr, D. A. (2017). Muscle development: Nucleating microtubules at the nuclear envelope. Curr. Biol. 27 (19), R1071–r1073. doi:10.1016/j.cub.2017.08.030
Stewart, C. L., Kozlov, S., Fong, L. G., and Young, S. G. (2007). Mouse models of the laminopathies. Exp. Cell Res. 313 (10), 2144–2156. doi:10.1016/j.yexcr.2007.03.026
Strelkov, S. V., Kreplak, L., Herrmann, H., and Aebi, U. (2004). Intermediate filament protein structure determination. Methods Cell Biol. 78, 25–43. doi:10.1016/s0091-679x(04)78002-4
Sun, T., Song, Y., Dai, J., Mao, D., Ma, M., Ni, J. Q., et al. (2019). Spectraplakin shot Maintains perinuclear microtubule organization in Drosophila polyploid cells. Dev. Cell 49 (5), 731–747. doi:10.1016/j.devcel.2019.03.027
Sweeney, H. L., and Hammers, D. W. (2018). Motor proteins. Cold Spring Harb. Perspect. Biol. 10 (2), a021931. doi:10.1101/cshperspect.a021931
Swift, J., Ivanovska, I. L., Buxboim, A., Harada, T., Dingal, P. C., Pinter, J., et al. (2013). Nuclear lamin-A scales with tissue stiffness and enhances matrix-directed differentiation. Science 341 (6149), 1240104. doi:10.1126/science.1240104
Sylvius, N., Bilinska, Z. T., Veinot, J. P., Fidzianska, A., Bolongo, P. M., Poon, S., et al. (2005). In vivo and in vitro examination of the functional significances of novel lamin gene mutations in heart failure patients. J. Med. Genet. 42 (8), 639–647. doi:10.1136/jmg.2004.023283
Tapscott, S. J., Davis, R. L., Thayer, M. J., Cheng, P. F., Weintraub, H., and Lassar, A. B. (1988). MyoD1: A nuclear phosphoprotein requiring a Myc homology region to convert fibroblasts to myoblasts. Science 242 (4877), 405–411. doi:10.1126/science.3175662
Technau, M., and Roth, S. (2008). The Drosophila KASH domain proteins Msp-300 and Klarsicht and the SUN domain protein Klaroid have no essential function during oogenesis. Fly. (Austin) 2 (2), 82–91. doi:10.4161/fly.6288
Tillery, M. M. L., Blake-Hedges, C., Zheng, Y., Buchwalter, R. A., and Megraw, T. L. (2018). Centrosomal and non-centrosomal microtubule-organizing centers (MTOCs) in Drosophila melanogaster. Cells 7 (9), E121. doi:10.3390/cells7090121
Turgay, Y., and Medalia, O. (2017). The structure of lamin filaments in somatic cells as revealed by cryo-electron tomography. Nucleus 8 (5), 475–481. doi:10.1080/19491034.2017.1337622
Vergarajauregui, S., Becker, R., Steffen, U., Sharkova, M., Esser, T., Petzold, J., et al. (2020). AKAP6 orchestrates the nuclear envelope microtubule-organizing center by linking golgi and nucleus via AKAP9. Elife 9, e61669. doi:10.7554/eLife.61669
Volk, T. (2013). Positioning nuclei within the cytoplasm of striated muscle fiber: Cooperation between microtubules and KASH proteins. Nucleus 4 (1), 18–22. doi:10.4161/nucl.23086
Walter, M. C., Witt, T. N., Weigel, B. S., Reilich, P., Richard, P., Pongratz, D., et al. (2005). Deletion of the LMNA initiator codon leading to a neurogenic variant of autosomal dominant Emery-Dreifuss muscular dystrophy. Neuromuscul. Disord. 15 (1), 40–44. doi:10.1016/j.nmd.2004.09.007
Wang, N., Tytell, J. D., and Ingber, D. E. (2009). Mechanotransduction at a distance: Mechanically coupling the extracellular matrix with the nucleus. Nat. Rev. Mol. Cell Biol. 10 (1), 75–82. doi:10.1038/nrm2594
Wang, S., Reuveny, A., and Volk, T. (2015). Nesprin provides elastic properties to muscle nuclei by cooperating with spectraplakin and EB1. J. Cell Biol. 209 (4), 529–538. doi:10.1083/jcb.201408098
Wang, W., Shi, Z., Jiao, S., Chen, C., Wang, H., Liu, G., et al. (2012). Structural insights into SUN-KASH complexes across the nuclear envelope. Cell Res. 22 (10), 1440–1452. doi:10.1038/cr.2012.126
Wilson, K. L., and Foisner, R. (2010). Lamin-binding proteins. Cold Spring Harb. Perspect. Biol. 2 (4), a000554. doi:10.1101/cshperspect.a000554
Wiltshire, K. M., Hegele, R. A., Innes, A. M., and Brownell, A. K. (2013). Homozygous lamin A/C familial lipodystrophy R482Q mutation in autosomal recessive Emery Dreifuss muscular dystrophy. Neuromuscul. Disord. 23 (3), 265–268. doi:10.1016/j.nmd.2012.11.011
Wong, X., Hoskins, V. E., Melendez-Perez, A. J., Harr, J. C., Gordon, M., and Reddy, K. L. (2021). Lamin C is required to establish genome organization after mitosis. Genome Biol. 22 (1), 305. doi:10.1186/s13059-021-02516-7
Wong, X., Melendez-Perez, A. J., and Reddy, K. L. (2022). The nuclear lamina. Cold Spring Harb. Perspect. Biol. 14 (2), a040113. doi:10.1101/cshperspect.a040113
Worman, H. J., and Bonne, G. (2007). Laminopathies": A wide spectrum of human diseases. Exp. Cell Res. 313 (10), 2121–2133. doi:10.1016/j.yexcr.2007.03.028
Worman, H. J. (2012). Nuclear lamins and laminopathies. J. Pathol. 226 (2), 316–325. doi:10.1002/path.2999
Xie, X., and Fischer, J. A. (2008). On the roles of the Drosophila KASH domain proteins Msp-300 and Klarsicht. Fly. (Austin) 2 (2), 74–81. doi:10.4161/fly.6108
Yu, J., Starr, D. A., Wu, X., Parkhurst, S. M., Zhuang, Y., Xu, T., et al. (2006). The KASH domain protein MSP-300 plays an essential role in nuclear anchoring during Drosophila oogenesis. Dev. Biol. 289 (2), 336–345. doi:10.1016/j.ydbio.2005.10.027
Zhang, Q., Bethmann, C., Worth, N. F., Davies, J. D., Wasner, C., Feuer, A., et al. (2007). Nesprin-1 and -2 are involved in the pathogenesis of Emery Dreifuss muscular dystrophy and are critical for nuclear envelope integrity. Hum. Mol. Genet. 16 (23), 2816–2833. doi:10.1093/hmg/ddm238
Zhang, Q., Skepper, J. N., Yang, F., Davies, J. D., Hegyi, L., Roberts, R. G., et al. (2001). Nesprins: A novel family of spectrin-repeat-containing proteins that localize to the nuclear membrane in multiple tissues. J. Cell Sci. 114 (24), 4485–4498. doi:10.1242/jcs.114.24.4485
Zheng, Y., Buchwalter, R. A., Zheng, C., Wight, E. M., Chen, J. V., and Megraw, T. L. (2020). A perinuclear microtubule-organizing centre controls nuclear positioning and basement membrane secretion. Nat. Cell Biol. 22 (3), 297–309. doi:10.1038/s41556-020-0470-7
Keywords: myonuclei, muscular dystrophies, Drosophila, lamins, LINC complex, microtubules, muscle
Citation: Shaw NM, Rios-Monterrosa JL, Fedorchak GR, Ketterer MR, Coombs GS, Lammerding J and Wallrath LL (2022) Effects of mutant lamins on nucleo-cytoskeletal coupling in Drosophila models of LMNA muscular dystrophy. Front. Cell Dev. Biol. 10:934586. doi: 10.3389/fcell.2022.934586
Received: 02 May 2022; Accepted: 20 July 2022;
Published: 31 August 2022.
Edited by:
Ming Guo, Massachusetts Institute of Technology, United StatesReviewed by:
Alexei Arnaoutov, Eunice Kennedy Shriver National Institute of Child Health and Human Development (NIH), United StatesJeremy T. Smyth, Uniformed Services University of the Health Sciences, United States
Copyright © 2022 Shaw, Rios-Monterrosa, Fedorchak, Ketterer, Coombs, Lammerding and Wallrath. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Lori L. Wallrath, Lori-wallrath@uiowa.edu