Cell therapy involves the utilization of cells that are cultured and/or modified in vitro, and then transplanted into patients to address their medical conditions or replace diseased, lost, or malfunctioning cells and tissues. This therapeutic approach encompasses a range of cell types, such as stem cells, matured cells, engineered tissues, engineered cells and T cells. These cells are often cultured in vitro to facilitate their proliferation and expansion in number. In some cases, they are also differentiated or genetically engineered in the laboratory before being introduced to patients through either autologous or allogeneic transplantations. This Research Topic explores various methods and technologies employed to engineer cells, as well as how to optimize their expansion and culture conditions for practical applications.For cell therapy to be effective, cells must have specific genotypes, phenotypes, and functions, as well as be in sufficient quantities. For example, stem cells, including pluripotent and multipotent stem cells, possess the remarkable capability to divide and multiply in vitro or differentiate into matured cells of specific tissue lineages. The main challenge currently faced in cell therapy is the ability to induce desired cellular responses that promote cell multiplication, generation of specific genotypes and phenotypes, and/or the production of protein products in large volumes. In the case of cell therapy involving three-dimensional tissues, the cells must be cultured in three-dimensional constructs, which may present challenges in terms of mass transfer. This means that culture media must effectively diffuse into the core of the tissues. To tackle these issues, innovative technologies and protocols are required to generate cells of various types in sufficient quantities and desired genotypes and phenotypes. Moreover, when these cells are transplanted in vivo for cell therapy, they need to effectively integrate with the in vivo environment.This Research Topic specifically centers around cells involved in therapy, including both non-genetically modified and genetically engineered cells, stem cells, differentiated stem cells, T cells, other immune cells and cells that are engineered to express therapeutic substances. We welcome submissions of Original Research articles and Reviews covering a wide range of subtopics, including but not limited to：• The investigation of single cells or cells within three-dimensional tissue constructs utilized in the field of regenerative medicine;• Scaffold design and fabrication for cell proliferation, differentiation and/or tissue formation;• Methods and solutions aimed at promoting vascularization and innervation of three-dimensional tissue constructs to facilitate cell proliferation or specific functions;• Studies that delve into genetic engineering, the development of cell culture media formulation, cell culture conditions, and the design and development of bioreactor and bioprocess.
Cell therapy involves the utilization of cells that are cultured and/or modified in vitro, and then transplanted into patients to address their medical conditions or replace diseased, lost, or malfunctioning cells and tissues. This therapeutic approach encompasses a range of cell types, such as stem cells, matured cells, engineered tissues, engineered cells and T cells. These cells are often cultured in vitro to facilitate their proliferation and expansion in number. In some cases, they are also differentiated or genetically engineered in the laboratory before being introduced to patients through either autologous or allogeneic transplantations. This Research Topic explores various methods and technologies employed to engineer cells, as well as how to optimize their expansion and culture conditions for practical applications.For cell therapy to be effective, cells must have specific genotypes, phenotypes, and functions, as well as be in sufficient quantities. For example, stem cells, including pluripotent and multipotent stem cells, possess the remarkable capability to divide and multiply in vitro or differentiate into matured cells of specific tissue lineages. The main challenge currently faced in cell therapy is the ability to induce desired cellular responses that promote cell multiplication, generation of specific genotypes and phenotypes, and/or the production of protein products in large volumes. In the case of cell therapy involving three-dimensional tissues, the cells must be cultured in three-dimensional constructs, which may present challenges in terms of mass transfer. This means that culture media must effectively diffuse into the core of the tissues. To tackle these issues, innovative technologies and protocols are required to generate cells of various types in sufficient quantities and desired genotypes and phenotypes. Moreover, when these cells are transplanted in vivo for cell therapy, they need to effectively integrate with the in vivo environment.This Research Topic specifically centers around cells involved in therapy, including both non-genetically modified and genetically engineered cells, stem cells, differentiated stem cells, T cells, other immune cells and cells that are engineered to express therapeutic substances. We welcome submissions of Original Research articles and Reviews covering a wide range of subtopics, including but not limited to：• The investigation of single cells or cells within three-dimensional tissue constructs utilized in the field of regenerative medicine;• Scaffold design and fabrication for cell proliferation, differentiation and/or tissue formation;• Methods and solutions aimed at promoting vascularization and innervation of three-dimensional tissue constructs to facilitate cell proliferation or specific functions;• Studies that delve into genetic engineering, the development of cell culture media formulation, cell culture conditions, and the design and development of bioreactor and bioprocess.