Non-coding RNAs in Neurodegenerative Diseases

Editorial

13 July 2023

Editorial: Non-coding RNAs in neurodegenerative diseases

Mingshu Mo

1,717 views

0 citations

Editors

Mingshu Mo

The First Affiliated Hospital Of Guangzhou Medical University

Yong U. Liu

Guangzhou First People's Hospital

Jiyun Peng

Nanchang University

Shaomin Li

Brigham and Women's Hospital, Harvard Medical School

Impact

Original Research

15 November 2023

Circulating plasma miR-23b-3p as a biomarker target for idiopathic Parkinson's disease: comparison with small extracellular vesicle miRNA

Sanskriti Rai

, 15 more and

Saroj Kumar

2,430 views

5 citations

The dysregulation of miRNAs can contribute to neurodegeneration in ALS motor neurons related to several molecular pathways.

Mini Review

21 February 2023

Implications of miRNAs dysregulation in amyotrophic lateral sclerosis: Challenging for clinical applications

Yuka Koike

and

Osamu Onodera

4,222 views

6 citations

Review

16 February 2023

The emerging role of circular RNAs in Parkinson’s disease

Jiajia Liao

, 6 more and

Yousheng Xiao

1,857 views

6 citations

Original Research

12 November 2021

Study of Neuronal Apoptosis ceRNA Network in Hippocampal Sclerosis of Human Temporal Lobe Epilepsy by RNA-Seq

Shengkun Yu

, 10 more and

Zhiguo Lin

Enrichment results of KEGG pathway: (A) Apoptosis pathway of DEmRNAs in down-regulated ceRNA network. (B) MAPK pathway of DEmRNAs in down-regulated ceRNA network. Orange module indicates the enriched DEmRNAs in the apoptosis and MAPK pathway.

Hippocampal sclerosis (HS) is one of the most common pathological type of intractable temporal lobe epilepsy (TLE), often characterized by hippocampal atrophy, neuronal apoptosis, and gliogenesis. However, the molecular mechanisms of neuronal apoptosis in patients with HS are still not fully understood. We therefore conducted a pilot study focusing on the neuronal apoptosis ceRNA network in the sclerotic hippocampus of intractable TLE patients. In this research, RNA sequencing (RNA-seq) was utilized to quantify the expression levels of lncRNAs, miRNAs, and mRNAs in TLE patients with HS (HS-TLE) and without HS (non-HS-TLE), and reverse transcription-quantitative PCR (qRT-PCR). The interactions of differential expression (DE) lncRNAs-miRNAs or DEmiRNAs-mRNAs were integrated by StarBase v3.0, and visualized using Cytoscape. Subsequently, we annotate the functions of lncRNA-associated competitive endogenous RNA (ceRNA) network through analysis of their interactions with mRNAs. RNA-seq analyses showed 381 lncRNAs, 42 miRNAs, and 457 mRNAs were dysregulated expression in HS-TLE compared to non-HS-TLE. According to the ceRNA hypothesis, 5 HS-specific ceRNA network were constructed. Among them, the core ceRNA regulatory network involved in neuronal apoptosis was constituted by 10 DElncRNAs (CDKN2B-AS1, MEG3, UBA6-AS1, etc.), 7 DEmiRNAs (hsa-miR-155-5p, hsa-miR-195-5p, hsa-miR-200c-3p, etc.), and 3 DEmRNAs (SCN2A, DYRK2, and MAPK8), which belonging to apoptotic and epileptic terms. Our findings established the first ceRNA network of lncRNA-mediated neuronal apoptosis in HS-TLE based on transcriptome sequencing, which provide a new perspective on the disease pathogenesis and precise treatments of HS.

4,196 views

13 citations

Biogenesis of circRNAs. (A) Direct back-splicing occurs when an upstream intron and downstream intron pair with each other through complementarity, forming ecircRNAs or EIciRNAs. (B) The process of exon skipping begins with the splicing of pre-mRNA. Then, a lariat containing the exon (yellow) and introns is produced, after which an ecircRNA is generated. (C) The formation of ciRNA depends on two significant RNA elements (yellow and red), which enable an intron to escape debranching and degradation.

Review

02 February 2023

Circular RNAs: New players involved in the regulation of cognition and cognitive diseases

Xiaohan Yu

, 4 more and

Yue Wang

2,353 views

2 citations

The validation results of circRalgapa1, circPsd3, circPpme1, circKansl1l, circRnf150, Slco1b2, Ino80d, Chrnb1, Sncg, and Agt by qRT-PCR in CON and PND groups (n = 10, each group). *P < 0.05 compared with the CON group, **P < 0.01 compared with the CON group, ****P < 0.005 compared with the CON group.

Original Research

24 June 2021

Identification of the Potential Gene Regulatory Networks and Therapeutics in Aged Mice With Postoperative Neurocognitive Disorder

Wensi Wu

, 7 more and

Zhi Wang

Postoperative neurocognitive disorder (PND) is one of the most common postoperative neurological complications in aged patients, characterized by mental disorder, anxiety, personality changes, and impaired memory. At present, the molecular mechanism of PND remains largely unclear, and the ideal biomarker for clinical diagnosis and prognosis are lacking. Circular RNA (circRNA) and microRNA (miRNA), as unique non-coding RNAs, affecting the regulation of miRNAs on genes and further intervening in the progression of diseases through the sponge action between the two. Besides, it could be served as novel biomarkers in various diseases. In order to detect the differential expression profiles of genes caused by PND, a total of 26 18-month-old male C57BL/6 mice were randomly assigned to control group and PND group. Behavioral tests showed that mice in the PND group had impaired cognitive function compared with the control group. Three mice in each group were randomly selected to harvest the brain for analysis the expressions of circRNAs, miRNAs, and mRNAs in the prefrontal cortex by next-generation sequencing (NGS) technology. Differentially expressed genes, including 1192 circRNAs, 27 miRNAs, and 266 mRNAs were identified, and its accuracy was further confirmed by qRT-PCR. Bioinformatics analysis results suggested that neuroinflammation was the main pathological mechanism of PND. The construction of competitive endogenous RNA (ceRNA) networks and the identification of hub genes provided possible therapeutic targets for PND. Cinnarizine and Clemastine were predicted to have the potential therapeutic effects on PND. This is the first study to explore the differential expression profiles of genes and their regulation mechanisms in PND, our results provided new clues and targets for the treatment of this refractory disease.

3,013 views

11 citations