The Immunomodulatory Role of Fibroblast in Tissue Immunopathology

Editors

Chao Li

China Medical University

Lushen Li

University of Maryland

Impact

HDAC3 interacts directly or indirectly with a number of transcriptional regulatory proteins (from STRING.COM).

Review

26 September 2024

The involvement of HDAC3 in the pathogenesis of lung injury and pulmonary fibrosis

Hanming Yu

, 2 more and

Xiu Gu

3,323 views

2 citations

(A) Representative gating of TLR9 expression in FRC subsets. (B-E) WT mice were intraperitoneally treated with PBS (Cont), ODN1585 (ODN, 2.5 nmol/mouse, LPS (5mg/kg), or ODN+LPS for 18 hours. (B) Mean fluorescence intensity of TLR9 in FRC subsets. (C) Numbers of FRCs, (D) Numbers of CD55hi and CD55lo FRCs, and (E) The frequency of Ki67+ FRC in the CD55hi and CD55lo subset in the mesenteric adipose tissue. (F-H) Tlr9fl/fl and Ccl19creTlr9fl/fl mice were subjected to CLP for 18h. (F) Numbers of FRCs, (G) Numbers of CD55hi and CD55lo FRCs, and (H) The frequency of Ki67+ FRC in the CD55hi and CD55lo subset in the mesenteric adipose tissue. Data are pooled from 2 separate experiments. Data are shown as mean ± SD. Symbols represent individual mice. Statistical differences were determined using two-way ANOVA adjusted by Bonferroni’s testing. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Original Research

17 May 2024

Activation of TLR9 signaling suppresses the immunomodulating functions of CD55lo fibroblastic reticular cells during bacterial peritonitis

Ting Jiang

, 4 more and

Meihong Deng

1,497 views

1 citations

Original Research

22 April 2024

Relationship between fibroblast growth factor in plasma and carotid plaque neovascularization: a pilot study

Mahtab Zamani

, 5 more and

Mona Skjelland

1,241 views

0 citations

Mini Review

09 January 2023

Crosstalk between fibroblasts and T cells in immune networks

Byunghyuk Lee

, 1 more and

Kihyuk Shin

Fibroblasts are primarily considered as cells that support organ structures and are currently receiving attention for their roles in regulating immune responses in health and disease. Fibroblasts are assigned distinct phenotypes and functions in different organs owing to their diverse origins and functions. Their roles in the immune system are multifaceted, ranging from supporting homeostasis to inducing or suppressing inflammatory responses of immune cells. As a major component of immune cells, T cells are responsible for adaptive immune responses and are involved in the exacerbation or alleviation of various inflammatory diseases. In this review, we discuss the mechanisms by which fibroblasts regulate immune responses by interacting with T cells in host health and diseases, as well as their potential as advanced therapeutic targets.

4,965 views

11 citations

Original Research

11 July 2023

Identification of immune infiltration and cuproptosis-related molecular clusters in tuberculosis

Sijun Li

, 4 more and

Qingdong Zhu

3,283 views

12 citations

Original Research

19 December 2022

Appropriate level of cuproptosis may be involved in alleviating pulmonary fibrosis

Guoxing Li

, 4 more and

Gang Li

Objective: Cuproptosis is a newly discovered form of programmed cell death that has not been studied in pulmonary fibrosis. The purpose of the present study was to explore the relationship between cuproptosis and pulmonary fibrosis.

Methods: Single-cell sequencing (scRNA-seq) data for human and mouse pulmonary fibrosis were obtained online from Gene Expression Omnibus (GEO) database. First, fibroblast lineage was identified and extracted using the Seurat toolkit. The pathway was then evaluated via Gene Set Enrichment Analyses (GSEA), while transcription factor activity was analyzed using DoRothEA. Next, fibroblast differentiation trajectory was inferred via Monocle software and changes in gene expression patterns during fibroblast activation were explored through gene dynamics analysis. The trajectory was then divided into three cell states in pseudotime order and the expression level of genes related to cuproptosis promotion in each cell state was evaluated, in addition to genes related to copper export and buffering and key genes in cellular metabolic pathways.

Results: In the mouse model of pulmonary fibrosis induced by bleomycin, the genes related to cuproptosis promotion, such as Fdx1, Lias, Dld, Pdha1, Pdhb, Dlat, and Lipt1, were gradually down-regulated in the process of fibroblast differentiation from resting fibroblast to myofibroblast. Consistently, the same results were obtained via analysis of scRNA-seq data for human pulmonary fibrosis. In addition, genes related to copper ion export and buffering gradually increased with the activation of fibroblasts. Metabolism reprogramming was also observed, while fibroblast activation and tricarboxylic acid(TCA) cycle and lipid metabolism were gradually down-regulated and mitochondrial metabolism was gradually up-regulated.

Conclusion: The present study is the first to reveal a negative correlation between cuproptosis and fibrosis, suggesting that an appropriate cuproptosis level may be involved in inhibiting fibroblast activation. This may provide a new method for the treatment of pulmonary fibrosis.