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Original Research
13 April 2021
Absolute DNA methylation levels across miRNA primary transcript region in soybean nodules and roots at 12, 22, and 36 dpi. (A–I): Differences in global DNA methylation levels between nodules and roots over miRNA primary transcript region in the CG, CHG, and CHH sequence contexts at 12– (A–C), 22– (D–F), and 36–dpi (G–I). The vertical dotted flank the miRNA primary transcript region, and 0.15 kb indicates the distance from the middle of primary transcript region. (J–L): Boxplots demonstrating statistically significant differences in absolute methylation levels across miRNA primary transcript region between nodules and roots in various sequence contexts at 12 (J), 22 (K), and 36 dpi (L). Asterisks indicate p < 0.001 for the comparison between nodules and roots as determined by t test.
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