Antibodies are widely used in clinical and academic settings where the high antigen-antibody specificity is desired, such as disease treatment and antigen quantitative immunoassay. The application of antibody medications has become an important part of the biotechnology and pharmaceutical industries. Research into this field has been greatly improved by the advent of genetically engineered antibodies. These antibody molecules are modified through antibody engineering according to human design to remove, reduce, or replace irrelevant structures, to overcome diverse biological constraints while retaining or increasing the specificity and main biological activity of the natural antibody. Therefore, genetically engineered antibodies, including humanized and fully human antibodies, single-chain Fv, bi-specific and VHH, have many more potential applications than natural antibodies. Moreover, as probes, antibodies can also be used to study the relationship between the structure and function of antigens at different levels from molecules, cells and organs, and then to elucidate their mechanism theoretically.
Immunoassay technologies are highly sensitive and specific, and have a wide range of applications, such as in diagnosis, efficacy evaluation, pathogenesis of immune diseases, and food safety detection. In addition, labeling antibodies or antigens with fluorescein, nanomaterials, isotopes, or enzymes are widely used immunoassay techniques for sensitive detection of antigens or antibodies. For instance, immuno-fluorescence is widely used in the diagnosis of infectious diseases, radioimmunoassay (RIA) is used to detect hormones, enzyme-linked immunosorbent assay (ELISA) and lateral flow immunoassay (LFIA) are universal tools applied in many medical and academic settings. Furthermore, nanoparticles, such as gold nanospheres, gold nanoflowers, and latex microspheres are often used as the signal intensity reporter during the development of immuno-probes for lateral flow immunoassay.
The aim of this Research Topic is to attract contributions in the antibody area to highlight the latest developments in antibody engineering and technology for application, with particular focus on:
- Antibody preparation: monoclonal antibody, gene engineering antibody, scFv, Fab, nanobody (VHH)
- Antibody humanization
- Antibody labelling
- Antibody application for immunological assay
- Antibody application for clinical cure
- Sensors based on nanoparticles and antibody
- Assembly of functional antibodies
- Application of nanoparticle-antibody conjugates
- Antibody developability
- Design of antibody-based therapeutics
- Activity and structure of antibody
We welcome contributions on the antibody engineering and technology for application, including both original research papers, as well as mini reviews/perspectives and review papers.
Antibodies are widely used in clinical and academic settings where the high antigen-antibody specificity is desired, such as disease treatment and antigen quantitative immunoassay. The application of antibody medications has become an important part of the biotechnology and pharmaceutical industries. Research into this field has been greatly improved by the advent of genetically engineered antibodies. These antibody molecules are modified through antibody engineering according to human design to remove, reduce, or replace irrelevant structures, to overcome diverse biological constraints while retaining or increasing the specificity and main biological activity of the natural antibody. Therefore, genetically engineered antibodies, including humanized and fully human antibodies, single-chain Fv, bi-specific and VHH, have many more potential applications than natural antibodies. Moreover, as probes, antibodies can also be used to study the relationship between the structure and function of antigens at different levels from molecules, cells and organs, and then to elucidate their mechanism theoretically.
Immunoassay technologies are highly sensitive and specific, and have a wide range of applications, such as in diagnosis, efficacy evaluation, pathogenesis of immune diseases, and food safety detection. In addition, labeling antibodies or antigens with fluorescein, nanomaterials, isotopes, or enzymes are widely used immunoassay techniques for sensitive detection of antigens or antibodies. For instance, immuno-fluorescence is widely used in the diagnosis of infectious diseases, radioimmunoassay (RIA) is used to detect hormones, enzyme-linked immunosorbent assay (ELISA) and lateral flow immunoassay (LFIA) are universal tools applied in many medical and academic settings. Furthermore, nanoparticles, such as gold nanospheres, gold nanoflowers, and latex microspheres are often used as the signal intensity reporter during the development of immuno-probes for lateral flow immunoassay.
The aim of this Research Topic is to attract contributions in the antibody area to highlight the latest developments in antibody engineering and technology for application, with particular focus on:
- Antibody preparation: monoclonal antibody, gene engineering antibody, scFv, Fab, nanobody (VHH)
- Antibody humanization
- Antibody labelling
- Antibody application for immunological assay
- Antibody application for clinical cure
- Sensors based on nanoparticles and antibody
- Assembly of functional antibodies
- Application of nanoparticle-antibody conjugates
- Antibody developability
- Design of antibody-based therapeutics
- Activity and structure of antibody
We welcome contributions on the antibody engineering and technology for application, including both original research papers, as well as mini reviews/perspectives and review papers.