Enzymes, as widely used biocatalysts with excellent chemo-, regio-, and enantioselectivity, exceed those of many chemical catalysts. A profound understanding of the reaction mechanism and how it is influenced by process conditions is essential for the successful implementation of a biocatalytic and biotransforming process. The enzymes are engineered to improve their performance, combined in reaction cascades to expand the reaction scope and integrated in whole cells to provide an optimal environment for the biosynthesis and biotransformation. Protein engineering is a promising tool for the design and construction of proteins with the desired properties through modifying existing genes or mining novel ones.
Current biotechnology offers a comprehensive arsenal of powerful approaches to discover, improve and apply enzymes for the production of value-added compounds. Structure-based enzyme engineering can be employed to enhance the activity of a bottleneck enzyme and alter its substrate specificity and product selectivity. Smart-computing strategy and machine learning algorithms have also been applied to protein engineering, especially in helping prediction of protein structures, improving enzyme stability/selectivity/solubility, and guiding rational protein design as well as other functions. Protein engineering, together with high-throughput screening system, successfully enhanced the screening efficiency of several enzymes from mutated library to meet specific requirements.
This research topic will focus specifically on Original Research articles and reviews based on protein engineering technology, including but not limited to the following scope:
• The exploration of enzyme and microbial resources for biosynthesis value-added compounds;
• Directed evolution in biotechnology, synthetic biology, and metabolic engineering;
• New biotechnological approaches in genomics, proteomics, and metabolomics;
• Biocatalysis, fermentations and immobilization of industrial enzymes;
• Structure-based enzyme engineering technology;
• High-throughput screening strategy for predominant mutants;
• Molecular dynamic in enzyme engineering.
Enzymes, as widely used biocatalysts with excellent chemo-, regio-, and enantioselectivity, exceed those of many chemical catalysts. A profound understanding of the reaction mechanism and how it is influenced by process conditions is essential for the successful implementation of a biocatalytic and biotransforming process. The enzymes are engineered to improve their performance, combined in reaction cascades to expand the reaction scope and integrated in whole cells to provide an optimal environment for the biosynthesis and biotransformation. Protein engineering is a promising tool for the design and construction of proteins with the desired properties through modifying existing genes or mining novel ones.
Current biotechnology offers a comprehensive arsenal of powerful approaches to discover, improve and apply enzymes for the production of value-added compounds. Structure-based enzyme engineering can be employed to enhance the activity of a bottleneck enzyme and alter its substrate specificity and product selectivity. Smart-computing strategy and machine learning algorithms have also been applied to protein engineering, especially in helping prediction of protein structures, improving enzyme stability/selectivity/solubility, and guiding rational protein design as well as other functions. Protein engineering, together with high-throughput screening system, successfully enhanced the screening efficiency of several enzymes from mutated library to meet specific requirements.
This research topic will focus specifically on Original Research articles and reviews based on protein engineering technology, including but not limited to the following scope:
• The exploration of enzyme and microbial resources for biosynthesis value-added compounds;
• Directed evolution in biotechnology, synthetic biology, and metabolic engineering;
• New biotechnological approaches in genomics, proteomics, and metabolomics;
• Biocatalysis, fermentations and immobilization of industrial enzymes;
• Structure-based enzyme engineering technology;
• High-throughput screening strategy for predominant mutants;
• Molecular dynamic in enzyme engineering.