"Epigenetics" is used to refer to heritable alterations of the genome that are not due to changes in DNA sequence. These alterations include those at the DNA level, histone protein level, and chromatin level. Many enzymes and factors have been extensively studied since their discovery; DNA methyltransferases, histone post-translation modification enzymes , and chromatin loop factors are just a few examples .
However, the details surrounding how epigenetic factors complete their regulatory role have yet to be elaborated upon. There is an increasing amount of evidence to suggest that although there can be genome-wide epigenetic modifications, the expression pattern of the expected target genes was less dramatic than expected.
To elaborate epigenetics factors’ regulator roles, it’s important to distinguish their direct targets from indirect downstream targets. Advanced technologies could be applied to provide better evidence, for example, detect nascent RNA(SLAM-seq, TT-Seq, TimeLapse-Seq) changed in timely manner could reveal more direct targets, even transient targets. To induce quick changes, drug inhibitors, acute-inducible degron system(AID), proteolysis-targeting chimera system(PROTAC), very fast CRISPR (vfCRISPR) have shown their advantages in different aspects.
This Research Topic solicits novel "direct" relationship between Epigenetics factors and gene regulations. We welcome contributions in the form of Original Research Articles, Reviews, and Mini-Reviews that cover but are not limited to the following topics:
•Direct transcription targets of Epigenetics factors (include but not limited to : methyltransferase/ demethylase/ acetyltransferases/ deacetylases/ kinase/ phosphatases/ decrotonylase/ ubiquitinase/ sumoylase/ ribosylase/ biotinidase/ O-GlcNAcylase).
•Recruitment/Competent relationship between Transcription factor and Epigenetic factors.
•Post-Translational Modifications to Transcription factors by Epigenetic factors that could regulate their ability to form dimer or nuclear import and export .
•Methods measuring nascent RNA (such as SLAM-seq, TT-Seq, TimeLapse-Seq).
•Methods using acute perturbation of the genome (such as drug inhibitors, AID, PROTAC, vfCRISPR).
•Novel methods or approaches in the investigation of direct epigenetic regulation of gene expression.
"Epigenetics" is used to refer to heritable alterations of the genome that are not due to changes in DNA sequence. These alterations include those at the DNA level, histone protein level, and chromatin level. Many enzymes and factors have been extensively studied since their discovery; DNA methyltransferases, histone post-translation modification enzymes , and chromatin loop factors are just a few examples .
However, the details surrounding how epigenetic factors complete their regulatory role have yet to be elaborated upon. There is an increasing amount of evidence to suggest that although there can be genome-wide epigenetic modifications, the expression pattern of the expected target genes was less dramatic than expected.
To elaborate epigenetics factors’ regulator roles, it’s important to distinguish their direct targets from indirect downstream targets. Advanced technologies could be applied to provide better evidence, for example, detect nascent RNA(SLAM-seq, TT-Seq, TimeLapse-Seq) changed in timely manner could reveal more direct targets, even transient targets. To induce quick changes, drug inhibitors, acute-inducible degron system(AID), proteolysis-targeting chimera system(PROTAC), very fast CRISPR (vfCRISPR) have shown their advantages in different aspects.
This Research Topic solicits novel "direct" relationship between Epigenetics factors and gene regulations. We welcome contributions in the form of Original Research Articles, Reviews, and Mini-Reviews that cover but are not limited to the following topics:
•Direct transcription targets of Epigenetics factors (include but not limited to : methyltransferase/ demethylase/ acetyltransferases/ deacetylases/ kinase/ phosphatases/ decrotonylase/ ubiquitinase/ sumoylase/ ribosylase/ biotinidase/ O-GlcNAcylase).
•Recruitment/Competent relationship between Transcription factor and Epigenetic factors.
•Post-Translational Modifications to Transcription factors by Epigenetic factors that could regulate their ability to form dimer or nuclear import and export .
•Methods measuring nascent RNA (such as SLAM-seq, TT-Seq, TimeLapse-Seq).
•Methods using acute perturbation of the genome (such as drug inhibitors, AID, PROTAC, vfCRISPR).
•Novel methods or approaches in the investigation of direct epigenetic regulation of gene expression.