Signalling pathways consist of a series of complex macromolecular interactions that are tightly spatially and temporally regulated and allow signal propagation, amplification and modulation that ultimately leads to a cellular response to stimuli. This research topic aims to provide an overview of existing and emerging experimental and theoretical approaches to study protein-protein interactions (PPIs) that would shed light on the intricate architecture of the transmembrane receptor signalling interactome. We welcome submissions (reviews, research articles, methods, perspectives/opinions, etc.) that address, but are not limited to, the following areas of PPI investigation:
- progress in biophysical methods for monitoring PPIs in living cells based on fluorescence/bioluminescence resonance energy transfer (FRET, BRET, and variants thereof),
- receptor monomers vs. receptor homo- and hetero-oligomerization (functional significance, negative cooperativity, dimer asymmetry, plasma membrane localization, interaction with the cytoskeleton),
- experimental approaches for monitoring dynamic/transient interactions among signalling partners within distinct cellular compartments (e.g., NMR, EPR, single molecule FRET, super-resolution microscopy),
- bioinformatics and computational approaches for the prediction of PPIs and the management/mining of large data sets,
- receptor screening challenges (PPIs-based screening platforms for medium/high throughput screening of compounds),
- biosensor development and its application in living subjects or organoid cultures (advancement of personalized medicine),
- experimental challenges and future trends of PPI research in the field of receptor biology.
This collection will be compiled in cooperation with
ERNEST COST Action.
Important Note: All contributions to this Research Topic must be within the scope of the section and journal to which they are submitted, as defined in their mission statements. Frontiers reserves the right to guide an out-of-scope manuscript to a more suitable section or journal at any stage of peer review.