AUTHOR=Evans Christopher Thomas , Payton Oliver , Picco Loren , Allen Michael J. 

TITLE=Visualisation of microalgal-viral interactions by high-speed atomic force microscopy

JOURNAL=Frontiers in Virology

VOLUME=3

YEAR=2023

URL=https://www.frontiersin.org/journals/virology/articles/10.3389/fviro.2023.1111335

DOI=10.3389/fviro.2023.1111335

ISSN=2673-818X

ABSTRACT=<p>Visualization of viruses and their hosts has been paramount to their study and understanding. The direct observation of the morphological dynamics of infection is a highly desired capability and the focus of instrument development across a variety of microscopy technologies. This study demonstrates progress that has been made in exploiting the capabilities offered by HS-AFM to characterise the interactions between coccolithoviruses and their globally important coccolithophore hosts. We observe whole Emiliania huxleyi Virus capsids, transient binding to <italic>Emiliania huxleyi</italic> derived supported lipid bilayers, and host-virus binding in real-time in an environmentally relevant, aqueous environment.</p>