AUTHOR=Xufré Cristina , González Tanía , Leal Lorna , Trubey Charles M. , Lifson Jeffrey D. , Gatell José María , Alcamí José , Climent Núria , García Felipe , Sánchez-Palomino Sonsoles 

TITLE=Highly Efficient Autologous HIV-1 Isolation by Coculturing Macrophage With Enriched CD4+ T Cells From HIV-1 Patients

JOURNAL=Frontiers in Virology

VOLUME=2

YEAR=2022

URL=https://www.frontiersin.org/journals/virology/articles/10.3389/fviro.2022.869431

DOI=10.3389/fviro.2022.869431

ISSN=2673-818X

ABSTRACT=<p>We described a novel HIV autologous isolation method based in coculturing macrophages and CD4<sup>+</sup>T-cell-enriched fractions from peripheral blood collected from antiretroviral-treated (ART) HIV patients. This method allows the isolation of high viral titers of autologous viruses, over 10<sup>10</sup>HIV RNA copies/ml, and reduces the time required to produce necessary amounts for virus for use as antigens presented by monocyte-derived myeloid cells in HIV therapeutic vaccine approaches. By applying these high titer and autologous virus produced in the patient-derived cells, we intended to elicit a boost of the immunological system response in HIV therapeutic vaccines in clinical trials.</p>