Integrative metabolomics and proteomics reveal the effects and mechanisms of Salvia miltiorrhiza in alleviating traumatic blood stasis syndrome

Guo Pei¹Wang Xin²Dong Xufeng²Jiaguo Liu^1*Qin Zhihua^2*

• ¹Nanjing Agricultural University, Nanjing, Jiangsu Province, China
• ²Qingdao Agricultural University, Qingdao, Shandong Province, China

Salvia miltiorrhiza (SM) is widely used in clinical practice for the treatment of cardiovascular diseases. However, the efficacy and mechanisms of SM in addressing traumatic blood stasis syndrome (TBSS) have not been thoroughly investigated. We established a TBSS model in cats and examined the muscle swelling rate (MSR), pain index, coagulation index, hematological parameters, inflammatory factors, and platelet function levels to assess the effects of SM. Subsequently, integrative metabolomics and proteomics were employed to elucidate the effects and mechanisms of SM in alleviating TBSS. The results demonstrate that the effect of SM was evaluated by establishing a cat model of TBSS. Administration of SM for 10 days effectively mitigated the significant increase in markers such as MSR, pain index, WBC, PLT, PCT, FIB, PAI-1, TNF-α, IL-6, IL-1β, TBX2, TXB2/6-Keto-PGF1α, β-TG, and PF4. Additionally, there was a significant decrease in APTT, PT, TT, t-PA, IL-10, 6-Keto-PGF1α, and FN. These findings suggest that SM regulates swelling and pain, inflammatory responses, coagulation and fibrinolytic system abnormalities, as well as platelet aggregation and activation. Through platelet metabolomic and proteomic analyses, it was found that SM inhibited the aggregation and activation processes of TBSS platelets by modulating physiological pathways, including tryptophan metabolism, purine metabolism, fatty acid metabolism, the complement and coagulation cascades, and platelet activation.