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ORIGINAL RESEARCH article
Front. Vet. Sci.
Sec. Veterinary clinical, anatomical, and comparative pathology
Volume 12 - 2025 | doi: 10.3389/fvets.2025.1578425
Morphological, Phenotypical and Molecular Characterization of Canine Lymphomas with Dual T-and B-Cell Markers Expression
Provisionally accepted
Giorgia Mezzalira 1
Valeria Martini 2*
Francesca Abramo 3 Selina Iussich 1 Margherita Orlandi 1 Luca Pazzini 1 Barbara Banco 1 Anna Maria Rambaldi 1 Alessandro Bisognoso 1 Filippo Arena 1 Giulio Cocciolo 1 Michele Marino 1 Maria Massaro 1
Luca Aresu 4- 1 MYLAV La Vallonea Veterinary Diagnostic Laboratory, Rho, Milan, Italy
- 2 Department of Veterinary Medicine and Animal Sciences, Faculty of Veterinary Medicine, University of Milan, Milan, Lombardy, Italy
- 3 Department of Veterinary Sciences, University of Pisa, Pisa, Tuscany, Italy
- 4 Department of Veterinary Sciences, University of Turin,, Grugliasco, Turin, Italy
Recent investigations have identified rare, phenotypically complex lymphoma variants, including cases exhibiting concurrent expression of T-and B-cell markers. These atypical presentations suggest perturbations in lymphoid differentiation programs or clonal evolution, necessitating more sophisticated diagnostic approaches. The concurrent expression of CD3 and CD20 in canine lymphomas represents a particularly compelling phenomenon documented across various anatomical sites. Molecular diagnostics, particularly PARR, have provided crucial insights into these phenotypically complex cases, revealing concurrent clonal rearrangements of both TCRγ and IgH in selected cases, further challenging traditional classification paradigms. Here, we report 33 cases of canine double-positive lymphoma, retrieved with a retrospective analysis of the MyLAV Diagnostic Laboratory electronic database. Specifically, we report results of an integrated approach combining WHO-based morphological classification, comprehensive IHC immunophenotyping with T-cell (CD3 and CD5) and B-cell markers (CD20 and PAX5), and PARR analysis.
Keywords: dog, Lymphoma, Immunohistochemistry, Parr, double phenotype
Received: 17 Feb 2025; Accepted: 03 Apr 2025.
Copyright: © 2025 Mezzalira, Martini, Abramo, Iussich, Orlandi, Pazzini, Banco, Rambaldi, Bisognoso, Arena, Cocciolo, Marino, Massaro and Aresu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Valeria Martini, Department of Veterinary Medicine and Animal Sciences, Faculty of Veterinary Medicine, University of Milan, Milan, 20122, Lombardy, Italy
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