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ORIGINAL RESEARCH article
Front. Vet. Sci.
Sec. Animal Nutrition and Metabolism
Volume 12 - 2025 | doi: 10.3389/fvets.2025.1577126
This article is part of the Research Topic Mitigating Pathogen Load and AMR in Animals via Prebiotics and Probiotics View all 7 articles
Parabacteroides goldsteinii mitigates the lipopolysaccharide-induced inflammatory response in IPEC-J2 cells
Provisionally accepted- 1 Key Laboratory of Animal Nutrition and Feed Science, Yunnan Agricultural University, Kunming, China
- 2 School of Agriculture and Life Sciences, Kunming University, Kunming, Yunnan Province, China
The misuse of antibiotics threatens animal health and food safety and limits the development of livestock and poultry farming. In animal husbandry, probiotics can be used as an alternative to antibiotics because of their environmental protection and high efficiency. Parabacteroides goldsteinii (PG) has positive anti-inflammatory effects in mice; however, its role in other animals remains unknown. We evaluated the anti-inflammatory effect of PG supernatant on lipopolysaccharide (LPS)induced IPEC-J2 cells and the underlying mechanism. The results showed that cell viability with the PG supernatant was highest at a 20-fold dilution ratio. Exposure of IPEC-J2 cells to LPS (1 μg/mL) significantly increased IL-6 and IL-8 concentrations, which triggered an inflammatory response. PG attenuated the LPS-induced inflammatory response by inhibiting the release of pro-inflammatory cytokines (IL-6) and promoting the release of anti-inflammatory cytokines (IL-10). Transcriptome analysis showed that the average number of clean reads in the LPS and LPS-PG groups was 39,311,061 and 38,085,237, respectively. In total, 2126 differentially expressed genes (DEGs) were identified between the LPS and LPS-PG groups, including 36 up-regulated and 2090 down-regulated genes. Some DEGs, such as IL-6R and NF-кB, are related to inflammation. GO analysis was used to annotate the functions of the DEGs, and the results showed that biological regulation, cellular anatomical entity, and binding were dominant. KEGG enrichment analysis showed that the DEGs were significantly enriched in the PI3K-AKT signaling pathway, protein export pathway, and antigen processing and presentation pathway. These results indicate that P. goldsteinii is a promising probiotic for maintaining and improving intestinal health in piglets.
Keywords: Parabacteroides goldsteinii, IPEC-J2 cell, lipopolysaccharide, Anti-inflammatory mechanism, PI3K-Akt pathway
Received: 15 Feb 2025; Accepted: 07 Apr 2025.
Copyright: © 2025 Zhang, Li, He, Guo, Ge, Pan and Dong. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Hongbin Pan, Key Laboratory of Animal Nutrition and Feed Science, Yunnan Agricultural University, Kunming, China
Cuilian Dong, School of Agriculture and Life Sciences, Kunming University, Kunming, Yunnan Province, China
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