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BRIEF RESEARCH REPORT article
Front. Vet. Sci.
Sec. Veterinary Infectious Diseases
Volume 12 - 2025 | doi: 10.3389/fvets.2025.1574667
A case report: isolation and identification of an AKAV strain in dairy cattle in China
Provisionally accepted
Miaomiao Zhang
Menghua DengSisi Zhao
Dengshuai ZhaoYajie Zheng
Limei Qin
Han Gao
Mengmeng Zhao*
Keshan Zhang*- Foshan University, Foshan, China
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Akabane disease is an arthropod-borne disease caused by Akabane virus (AKAV), which is characterized by abortion, premature birth, stillbirth, congenital arthrosis, and hydrocephalic anencephalic syndrome in pregnant cattle and sheep. The occurrence of AKAV was proved by RT-PCR amplification based on AKAV S fragment, virus isolation, cells inoculation, cytopathy, transmission electron microscopy, and gene sequencing. The PCR amplicon was about 850 bp and was sequenced, and molecular identification of AKAV was conducted through phylogenetic analysis of S gene sequence. The results indicated that AKAV isolated from cattle in this study was genetically close to the strain isolated from Rhizomys pruinosus in China, 2016. However, the outbreak in bamboo rats may have been a sporadic event. The probability that Akabane virus (AKAV) can spread in rodents and mammals is still uncertain and requires further investigation. Using transmission electron microscopy (TEM), AKAV particles displayed the typical morphology associated with bunyaviruses reported previously. Briefly, the AKAV infection in cattle has been confirmed. This case report highlights the necessity for enhanced surveillance and preventive measures to mitigate the potential impact on livestock health and productivity.
Keywords: Akabane virus (AKAV), Bovine abortion, molecular detection, phylogenetic analysis, Transmission Electron Microscopy (TEM)
Received: 11 Feb 2025; Accepted: 10 Apr 2025.
Copyright: © 2025 Zhang, Deng, Zhao, Zhao, Zheng, Qin, Gao, Zhao and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Mengmeng Zhao, Foshan University, Foshan, China
Keshan Zhang, Foshan University, Foshan, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.