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ORIGINAL RESEARCH article
Front. Vet. Sci.
Sec. Animal Nutrition and Metabolism
Volume 12 - 2025 | doi: 10.3389/fvets.2025.1563124
This article is part of the Research Topic Advancements in Synthetic Microbiomes for Enhancing Animal Health View all 8 articles
Impact of Different Concentrations of Biogas Slurry as a Fertilizer Substitute on the Structure and Gene Distribution of Microbial Communities in oilseed rape Soil
Provisionally accepted
Xue Yang 1
Kai Zhang 2 Yaying Chen 3 Cancai Zheng 4 Chunlong Lei 3 Xiaoqin Song 5 Wen Bin 2 Xin Yuan 6 Hanyang Liu 3* Jiawen Zhu 3*- 1 Chengdu Agriculture And Forestry Academy Of Sciences, Chengdu, China
- 2 Sichuan Academy of Grassland Science, Chengdu Institute of Biology, Chinese Academy of Sciences (CAS), Chengdu, Sichuan Province, China
- 3 Chengdu Academy of Agriculture and Forestry Sciences, Chengdu, Sichuan Province, China
- 4 Sichuan Provincial Planning and Construction Service Center, Chengdu, Sichuan Province, China
- 5 Dayi County Agriculture and Rural Bureau, Chengdu, Sichuan Province, China
- 6 Sichuan Agricultural University, Chengdu, China
Application of biogas slurry (BS) in paddy fields offers a sustainable approach to reducing environmental risks while efficiently utilizing nutrients from biogas residues. However, BS may harbor animal gut microorganisms and associated antibiotic resistance genes (ARGs) arising from antibiotic use during livestock breeding, potentially facilitating the spread of resistance genes in soil ecosystems. In this two-year field study, we investigated the effects of replacing chemical fertilizers with different proportions of BS on the microbial communities in oilseed rape soils. Soil bacterial community diversity and composition were systematically evaluated using Illumina MiSeq high throughput sequencing, focusing on interrelationships and changes at the genetic level. The predominant bacterial phyla identified were Proteobacteria, Actinobacteria, and Bacteroidetes, with no significant differences observed at the phylum or species level across treatment groups. Overall microbial diversity and community structure remained largely stable. Dimensionality reduction analysis further revealed only minor variations in the abundance of ARGs, mobile genetic elements (MGEs), and metal resistance genes (MRGs) among treatments. These findings suggest that, in the short term, substituting chemical fertilizers with BS has minimal effects on soil microbial community structure and resistance gene dynamics. This study highlights the potential of BS as a viable alternative to chemical fertilizers, contributing to reduced environmental risks in agriculture. Future research should investigate the longterm ecological safety of BS under different soil types and management practices to comprehensively evaluate its sustainability in agricultural systems.
Keywords: soil microorganisms, oilseed rape, sustainable agriculture, Fertilizer replacement, antibiotic resistance genes
Received: 19 Jan 2025; Accepted: 07 Mar 2025.
Copyright: © 2025 Yang, Zhang, Chen, Zheng, Lei, Song, Bin, Yuan, Liu and Zhu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Hanyang Liu, Chengdu Academy of Agriculture and Forestry Sciences, Chengdu, Sichuan Province, China
Jiawen Zhu, Chengdu Academy of Agriculture and Forestry Sciences, Chengdu, Sichuan Province, China
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