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BRIEF RESEARCH REPORT article

Front. Vet. Sci.
Sec. Veterinary Pharmacology and Toxicology
Volume 12 - 2025 | doi: 10.3389/fvets.2025.1540132

Regulation of Ofloxacin resistance in Escherichia coli strains causing calf diarrhea by quorum-sensing acyl-homoserine lactone signaling molecules

Provisionally accepted
Zi Wang Zi Wang 1Miao Sun Miao Sun 1Yongqiang Wang Yongqiang Wang 2*Jinchuan Shi Jinchuan Shi 1*Wei Gao Wei Gao 1*Dongxu Han Dongxu Han 1*Fanjun Zeng Fanjun Zeng 1*Ren San Ren San 3*Liyin Du Liyin Du 1*Hongxia Ma Hongxia Ma 4*Kai Liu Kai Liu 1*
  • 1 Inner Mongolia Minzu University, Tongliao, China
  • 2 Zhalantun Vocational College, Hulun Buir, China
  • 3 Tongliao Agricultural and Animal Product Quality Safety Center, Tongliao, China
  • 4 Jilin Agriculture University, Changchun, Jilin Province, China

The final, formatted version of the article will be published soon.

    Escherichia coli is a major pathogen responsible for calf diarrhea. However, it has developed resistance to many antimicrobial drugs for their inappropriate usage. The bacterial quorum sensing system transmits information between bacteria , it's important in regulating bacterial virulence, drug and acid resistance and so on. This system can found in Gram-negative bacteria and operates through acyl-homoserine lactone (AHL) signaling molecules. In this study, a type I quorum sensing AHL, N-Octanoyl-L-Homoserine lactone (C8), was added to E. coli growth medium to investigate its regulatory functions in drug resistance. After screening out the strains of E. coli that showed an obvious regulatory effect to the drug ofloxacin (OFX), transcriptomic sequencing was performed on the E. coli strains from the sub-inhibitory concentration group that concentration plus C8 group, and the control group.It shows that C8 significantly influenced resistance to OFX and the minimum inhibitory concentration of OFX in the tested strain was significantly increased. To Analyze transcriptome sequencing results identified 415 differentially expressed genes between the control and sub-inhibitory concentration groups, of which 201 were up-regulated and 214 were down. There were 125 differentially expressed genes between bacteria treated with a sub-inhibitory concentration of OFX and those treated with C8 , of which 102 were up-regulated and 23 were down. Finally, It found that to adding the C8 significantly increased the resistance of tested bacteria to OFX. Data from transcriptome sequencing on differently expressed genes helps to explain how the type I quorum sensing system controls drug resistance in E. coli.

    Keywords: Escherichia coli, Drug Resistance, Quorum-sensing molecule, Transcriptomic sequencing, Differentially expressed genes

    Received: 05 Dec 2024; Accepted: 07 Jan 2025.

    Copyright: © 2025 Wang, Sun, Wang, Shi, Gao, Han, Zeng, San, Du, Ma and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Yongqiang Wang, Zhalantun Vocational College, Hulun Buir, 162650, China
    Jinchuan Shi, Inner Mongolia Minzu University, Tongliao, China
    Wei Gao, Inner Mongolia Minzu University, Tongliao, China
    Dongxu Han, Inner Mongolia Minzu University, Tongliao, China
    Fanjun Zeng, Inner Mongolia Minzu University, Tongliao, China
    Ren San, Tongliao Agricultural and Animal Product Quality Safety Center, Tongliao, China
    Liyin Du, Inner Mongolia Minzu University, Tongliao, China
    Hongxia Ma, Jilin Agriculture University, Changchun, 130118, Jilin Province, China
    Kai Liu, Inner Mongolia Minzu University, Tongliao, China

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