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ORIGINAL RESEARCH article

Front. Vet. Sci.
Sec. Veterinary Pharmacology and Toxicology
Volume 12 - 2025 | doi: 10.3389/fvets.2025.1539448
This article is part of the Research Topic Natural Compounds in Veterinary Therapeutics View all articles

Normal butanol fraction of Polygonum hydropiper L. flavonoids reduces inflammation caused by PCV2 infections in cell and mouse models

Provisionally accepted
Yu-Heng Wei Yu-Heng Wei Shu-Mian Zhou Shu-Mian Zhou Wen Zhao Wen Zhao Qi Chen Qi Chen Qiu-Hua Wang Qiu-Hua Wang Meiling Yu Meiling Yu Ying-yi Wei Ying-yi Wei Tingjun Hu Tingjun Hu *
  • Guangxi University, Nanning, China

The final, formatted version of the article will be published soon.

    The normal butanol fraction of Polygonum hydropiper L. flavonoids (FNB) exhibits significant antiinflammatory effects. This study investigated FNB's impact on inflammatory responses induced by Porcine circovirus type 2 (PCV2) in cell and mouse models. An inflammatory model was established in RAW264.7 cells infected with varying PCV2 concentrations. And assigning both RAW264.7 cells and 108 SPF-grade KM mice to Control, PCV2, Rutin, and various dosages of FNB groups. Inflammatory factors such as Monocyte Chemoattractant Protein-1 (MCP-1), interleukin-6 (IL-6), IL-8, IL-10, Tumor Necrosis Factor-alpha (TNF-α), Reactive Oxygen Species (ROS), and Nitric Oxide (NO) were quantified using ELISA, RT-qPCR and immunohistochemistry. Results showed that a PCV2 titer of 10 4.5 TCID50/0.1 mL when applied to RAW264.7 cells effectively established an in vitro inflammatory model at 12 and 24 h post-infection. Following PCV2 infection, all the inflammatory factors displayed a significant increased both in culture supernatant and intracellular mRNA expression levels (p < 0.05 or p < 0.01), but these levels were reduced by FNB treatment (p < 0.05 or p < 0.01). In mouse sera post-PCV2 infection also showed elevated levels of IL-6, IL-8 IL-10, TNF-α, and MCP-1 (p < 0.05 or p < 0.01). Additionally, mRNA and protein levels for TNF-α, IL-8, IL-10, IL-6, and iNOS rose significantly in lung tissues (p < 0.01) but decreased with FNB treatment (p < 0.05 or p < 0.01). These findings suggest that FNB reduces inflammatory factor production and modulates the inflammatory response triggered by PCV2 infection, potentially enhancing host resistance against it.

    Keywords: Porcine circovirus type 2, normal butanol fraction of Polygonum hydropiper L. flavonoids, Inflammatory Response, RAW264.7 cells, Model establishment

    Received: 04 Dec 2024; Accepted: 13 Jan 2025.

    Copyright: © 2025 Wei, Zhou, Zhao, Chen, Wang, Yu, Wei and Hu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Tingjun Hu, Guangxi University, Nanning, China

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