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ORIGINAL RESEARCH article
Front. Vet. Sci.
Sec. Animal Nutrition and Metabolism
Volume 12 - 2025 | doi: 10.3389/fvets.2025.1534665
This article is part of the Research TopicMitigating Pathogen Load and AMR in Animals via Prebiotics and ProbioticsView all 7 articles
The Alleviating Effects and Mechanisms of Enterococcus faecium Kimate-X and Lactobacillus plantarum Kimate-F Combination on Canine Inflammatory Bowel Disease
Provisionally accepted- 1Nanjing Agricultural University, Nanjing, China
- 2Collage of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, Jiangxi Province, China
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This study investigates explores the mechanisms by which Enterococcus faecium Kimate-X and Lactobacillus plantarum Kimate-F, individually and combined, alleviate canine inflammatory bowel disease (IBD), a condition marked by chronic gut inflammation and dysbiosis. Conventional IBD treatments often cause side effects and antibiotic resistance, prompting interest in probiotic alternatives. This study aimed to evaluate the antibacterial, antiinflammatory, and immunomodulatory properties of these probiotics using in vitro, mouse, and canine models. Kimate-F exhibited strong antibacterial activityIn vitro assays showed Kimate-F's strong antibacterial activity against Escherichia coli, Salmonella enteritidis, and Yersinia enterocolitica, while Kimate-X significantly reducedexcelled in reducing NO and TNF-α levels in an LPS-induced inflammation model. In both DSS-induced mouse and canine colitis models, the combination of Kimate-X and Kimate-F effectively reduced alleviated symptoms, including weight loss, colon inflammation, and elevated serum inflammatory markers, while increasingenhancing IL-10 expression. Metagenomic analysis demonstratedrevealed increased microbial diversity, particularly with especially Bifidobacterium species enrichment, and revealed activation of metabolic pathways associated with nutrient absorption and immune regulation.in the combination group, alongside enriched metabolic pathways related to nutrient absorption and immune regulation. Additionally, The the probiotic combination modulated PPAR and AMPK signaling pathways and increased short-chain fatty acids (SCFAs) productionalso modulated the PPAR and AMPK signaling pathways and boosted short-chain fatty acid production in canine feces, promoting gut health. These findings demonstrate the 设置了格式: 字体: 倾斜
Keywords: Canine inflammatory bowel disease (IBD), Probiotics, Enterococcus faecium, Lactobacillus plantarum, Dysbiosis, Inflammation
Received: 26 Nov 2024; Accepted: 07 Apr 2025.
Copyright: © 2025 Zhang, Hu, Zhong, Chen, Xie, Chen and Yu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Rui Zhang, Nanjing Agricultural University, Nanjing, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.