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ORIGINAL RESEARCH article

Front. Vet. Sci.
Sec. Animal Reproduction - Theriogenology
Volume 12 - 2025 | doi: 10.3389/fvets.2025.1534638
This article is part of the Research Topic Recent developments in Animal Reproduction: combining research with practice View all 3 articles

Which is More Effective in Thawing Frozen Rooster Sperm: Varying Temperature or Duration?

Provisionally accepted
Mustafa Yiğit NİZAM Mustafa Yiğit NİZAM 1MURAT SELCUK MURAT SELCUK 2*Burcu Esin Burcu Esin 3
  • 1 Faculty of Veterinary Medicine, Dokuz Eylül University, Alsancak, İzmir, Türkiye
  • 2 Ondokuz Mayıs University, Samsun, Türkiye
  • 3 Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun, Samsun, Türkiye

The final, formatted version of the article will be published soon.

    Cryopreservation of poultry sperm is crucial for preserving genetic diversity and protecting endangered breeds. Rooster sperm is highly susceptible to thawing-related damage due to the high levels of polyunsaturated fatty acids in the plasma membrane. This study investigated how thawing temperatures affect rooster sperm quality, focusing on motility, morphology, and viability under different storage conditions. Frozen rooster semen samples were thawed at three temperatures: 37°C for 30 seconds, 60°C for 5 seconds, and 72°C for 5 seconds. After thawing, the samples were stored at 4°C for up to 48 hours. Sperm quality parameters, including motility, kinematic properties, abnormal morphology, and viability rates, were assessed at various time points using CASA. Post-thaw motility exhibited significant variation across the three thawing temperatures at both 24 and 48 hours (P < 0.05). Progressive motility and rapid progressive motility also differed significantly at 24 hours (P < 0.05). Sperm viability and morphology assessments demonstrated statistical differences at multiple time points (P < 0.05). The findings suggest thawing at 37°C and storing at 4°C for up to 24 hours optimizes motility and viability for short-term storage.

    Keywords: Cryopreservation, Post-thaw, Rooster, sperm, dry thawing

    Received: 26 Nov 2024; Accepted: 02 Jan 2025.

    Copyright: © 2025 NİZAM, SELCUK and Esin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: MURAT SELCUK, Ondokuz Mayıs University, Samsun, Türkiye

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.