Tannin alleviated reproductive dysfunction in pregnant ewes infected with Haemonchus contortus

Yi Fang ^1* Xin Li ^1* Hai Xiang ^2* Rong Liang ^1* Xinyu Han ^2* Rongzhen Zhong ² Hongyu Liu ^1*

• ¹ Jilin Agriculture University, Changchun, China
• ² Key Laboratory of Black Soil Conservation and Utilization, Jilin Academy of Agricultural Sciences (CAAS), Changchun, Jilin Province, China

Haemonchus contortus (H. contortus) infection has a significant impact on the health of pregnant ewes and adversely affects fetal development, highlighting the critical need for a non-toxic feed additive as an alternative and sustainable control strategy. Tannin is a kind of polyphenol compound, which has certain antiparasitic. The objective of this study was to evaluate the impact of dietary tannin supplementation on fecal egg count (FEC), packed cell volume (PCV), complete blood count (CBC), hormone levels, inflammatory markers, placental inflammation, and fetal growth and development in H. contortus infected pregnant ewes.Methods: Hulunbuir ewes were randomly divided into 3 groups: control group (CON), gastrointestinal nematode infection group (GIN) and tannin group, which was infected by H. contortus with tannin feeding therapy (TAN).After artificial insemination was completed, and the ewes were confirmed for pregnancy and infection, stools were collected for FEC, blood samples were collected for PCV and CBC, hormonal, and inflammation levels. The mRNA levels of hypothalamic-pituitary-ovarian axis-related hormone receptors and placental tissue inflammation genes were detected by RT-qPCR. Finally, fetal weights were measured and fetal ovarian tissue samples were taken for transcriptomic analysis.The results showed that tannins increased levels of gonadotropin-releasing hormone (GnRH), folliclestimulating hormone (FSH), luteinizing hormone (LH), estrogen (E2), progesterone (P4), human chorionic gonadotropin (hCG), red blood cell (RBC) counts, packed cell volume (PCV), and mRNA levels of gonadotropic axis receptors in pregnant ewes infected with H. contortus (p < 0.05). Additionally, tannin reduced fecal egg count (FEC), leukocyte counts, and mRNA levels of inflammatory markers (p < 0.05). In addition, fetal ovarian sequencing further showed that tannin may alleviate the delay in fetal growth and development induced by H. contortus infection (p < 0.05).In summary, tannins have anthelmintic effects, restore reproductive hormone levels in pregnant ewes, reduce inflammation levels, and alleviate fetal growth retardation caused by H. contortus infection. Therefore, tannin is a suitable potential alternative to antibiotics as a feed additive.