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ORIGINAL RESEARCH article

Front. Vet. Sci.
Sec. Veterinary Experimental and Diagnostic Pathology
Volume 12 - 2025 | doi: 10.3389/fvets.2025.1521705
This article is part of the Research Topic Emerging Diseases and Diagnostics in Poultry Production View all 8 articles

Epidemiological Investigation of Goose Circovirus Based on a Newly Developed Indirect ELISA Method

Provisionally accepted
Jialong Chen Jialong Chen 1Wenchang Xue Wenchang Xue 1Zhanxin Yao Zhanxin Yao 1Chao Wang Chao Wang 1Wanjun Zhu Wanjun Zhu 2He Wang He Wang 3Jipei Zhang Jipei Zhang 1YI TANG YI TANG 4Rongchang Liu Rongchang Liu 5Jidang Chen Jidang Chen 1,6*
  • 1 School of Animal Science and Technology, Foshan University, Foshan, Guangdong, China, 528225, Foshan, China
  • 2 WANMUZHOU Biotechnology Limited Company, Foshan, Guangdong Province, China
  • 3 SHENZHEN KINGKEY SMART AGRICULTURE TIMES CO., LTD, Shenzhen, China
  • 4 College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong Province, China
  • 5 Institute of Animal Husbandry and Veterinary, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian Province, China
  • 6 Agricultural Teaching and Research Bases, Foshan University, Foshan, China., Foshan, China

The final, formatted version of the article will be published soon.

    Goose circovirus (GoCV) is a recently identified pathogen in geese that is known to cause slow growth, feather disorder syndrome, and immunosuppression. Infection with GoCV may increase the risk of coinfections with multiple pathogens, leading to significant economic losses in the goose industry. However, due to a lack of serological detection methods, analysis of viral nucleic acids has been widely used in GoCV epidemiological surveys, which has limited accurate monitoring of the prevalence of GoCV. We developed and optimized an indirect ELISA method based on the prokaryotic-expressed recombinant GoCV capsid protein (△Cap-iELISA). The △Cap-iELISA was then used to test 349 goose serum samples collected from Guangdong, Shandong, and Fujian provinces during 2023 and 2024. The results showed that the positive rate of GoCV antibodies in the sampled geese was 71.06%. Further analysis indicated that the positive rate of GoCV antibodies increased with the age of the geese. These findings suggest that truncated recombinant proteins obtained through codon optimization are a viable strategy for obtaining Cap proteins. Our △Cap-iELISA is suitable for extensive application in serological diagnosis of GoCV.

    Keywords: Goose circovirus (GoCV), Capsid protein (Cap), Serological detection methods, Prokaryotic expression, Indirect ELISA

    Received: 02 Nov 2024; Accepted: 24 Jan 2025.

    Copyright: © 2025 Chen, Xue, Yao, Wang, Zhu, Wang, Zhang, TANG, Liu and Chen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Jidang Chen, School of Animal Science and Technology, Foshan University, Foshan, Guangdong, China, 528225, Foshan, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.