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ORIGINAL RESEARCH article

Front. Vet. Sci.
Sec. Veterinary Experimental and Diagnostic Pathology
Volume 12 - 2025 | doi: 10.3389/fvets.2025.1493660

Blood biochemistry changes in a minipig infarction model

Provisionally accepted
Denes Korosi Denes Korosi 1Agoston Goczo Agoston Goczo 2Noemi Varga Noemi Varga 3Rita Garamvolgyi Rita Garamvolgyi 1,4*Nándor Balogh Nándor Balogh 5Nelli Farkas Nelli Farkas 2Andras Vorobcsuk Andras Vorobcsuk 2
  • 1 Doctoral School in Animal Science, Kaposvar, Hungarian University of Agricultural and Life Sciences, Gödöllő, Hungary
  • 2 Medical School, University of Pécs, Pécs, Hungary
  • 3 Cardio-Econom Ltd., Gorcsony, Hungary
  • 4 Auvet Pharma Ltd., Kaposvar, Hungary
  • 5 Praxislab Ltd., Budapest, Hungary

The final, formatted version of the article will be published soon.

    Introduction. The present study aimed to assess changes of biochemical parameters during the adaptation of myocardial infarction model to a conventional Hungarian minipig breed. According to our hypothesis, changes in the blood level of the necroenzymes are not only related to the interventional procedure, but are also influenced by peri-procedural animal keeping and treatment conditions. Methods. Closed chest acute myocardial infarction (AMI) was induced by balloon occlusion for 90 minutes in the left anterior descendent coronary artery (LAD) in 24 adult, female Pannon minipigs followed by reperfusion. Blood samples were taken before AMI, and immediately after the reperfusion, during the cardiac magnetic resonance imaging (cMRI) on Day 3 and Day 30. AST (aspartate transaminase), ALT (alanine aminotransferase), CK (creatine kinase), LDH (lactate dehydrogenase), high-sensitivity Troponin I were determined.Results. While the parameters measured at baseline remained within physiological ranges, a notable elevation was seen in comparison to the results observed on Day 30. This phenomenon was evident in all the laboratory parameters tested, except hs-Troponin. The results for AST, ALT, LDH, and CK were statistically significant (p=0.011, p=0.001, p=0.013, and p=0.001, respectively). A statistically significant difference was observed between the baseline and 30-day AST/ALT ratio (p=0.00514).Discussion. The elevated levels of necroenzymes observed at baseline are likely to be a consequence of the physical and social stress imposed by the study design on the minipigs during the 72-hour period prior to intervention. It is essential to define the optimal timing of baseline blood tests in order to ensure the reliability of the biochemical profile in a large animal infarction model.

    Keywords: blood biochemicals1, cardiac markers2, closed chest infarct3, large animal model4, minipig5, stress6

    Received: 09 Sep 2024; Accepted: 06 Jan 2025.

    Copyright: © 2025 Korosi, Goczo, Varga, Garamvolgyi, Balogh, Farkas and Vorobcsuk. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Rita Garamvolgyi, Auvet Pharma Ltd., Kaposvar, Hungary

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