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ORIGINAL RESEARCH article
Front. Vet. Sci.
Sec. Veterinary Infectious Diseases
Volume 12 - 2025 |
doi: 10.3389/fvets.2025.1476232
This article is part of the Research Topic The application of new technologies such as new vaccines, therapeutic cytokines and antibodies, and antiviral drugs in the prevention and treatment of animal infectious diseases View all 12 articles
The preventive effect of probiotic L. plantarum X86 isolated from raw milk on S. aureus-induced mastitis in rats
Provisionally accepted
Xiu-lan Xie 1,2*
Mei Cao 3 Shi-Ying Yan 2 Hai-Hui Gao 1 Yu-wei Yang 1 Jia-Yi Zeng 2
Gang Zhang 4
Jian Zhao 2*- 1 Institute of Animal Science, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan, China
- 2 Key Laboratory of Biological Resources and Ecological Environment of the Ministry of Education, College of Life Science, Sichuan University, Chengdu, Sichuan Province, China
- 3 Core Laboratory, School of Medicine, Sichuan Provincial People's Hospital Affiliated to University of Electronic Science and Technology of China, Chengdu, China
- 4 Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Ningxia, China
Mastitis is the most common and challenging disease that affects dairy animal welfare and causes huge economic loss in dairy industry globally. Conventional antibiotic treatment of mastitis raised the drug resistance and unsuccessful therapy. As an alternative approach, probiotic lactobacilli had shown multifunctional effects against diseases. Lactobacillus strains against mastitis are worth screening and evaluating. In this study, milk-derived lactobacillus spp. from Ningxia, China were screened in vitro and the anti-mastitis effect of a candidate strain was evaluated through a S. aureusinduced rat mastitis model. The results showed that Lactobacillus plantarum X86 exhibited a high adhesion rate of MAC-T cells, presented the best probiotic properties, and demonstrated anti-S. aureus effects in vitro through comprehensive assessment. Furthermore, L. plantarum X86 alleviated pathological damage to the mammary gland, liver, and colon, inhibited the mRNA expression of proinflammatory cytokines factors IL-1β, IL-6, and TNF-α in mammary gland tissue; and increased the content of intestine SCFAs in a rat mastitis model induced by S. aureus.In conclusion, our results suggested that L. plantarum X86 could be a promising probiotic for the prevention and treatment of S. aureus-induced mastitis.
Keywords: raw milk, Lactobacillus plantarum X86, Staphylococcus aureus, Rat mastitis model, anti-inflammatory
Received: 05 Aug 2024; Accepted: 07 Feb 2025.
Copyright: © 2025 Xie, Cao, Yan, Gao, Yang, Zeng, Zhang and Zhao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Xiu-lan Xie, Institute of Animal Science, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan, China
Jian Zhao, Key Laboratory of Biological Resources and Ecological Environment of the Ministry of Education, College of Life Science, Sichuan University, Chengdu, 610065, Sichuan Province, China
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