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ORIGINAL RESEARCH article

Front. Vet. Sci.
Sec. Animal Nutrition and Metabolism
Volume 12 - 2025 | doi: 10.3389/fvets.2025.1462287
This article is part of the Research Topic Omics Research in Canine and Feline Microbiome: Implications for Veterinary Medicine and Companion Animal Health View all 6 articles

Impact of Saccharomyces cerevisiae on the intestinal microbiota of dogs with antibiotic-induced dysbiosis

Provisionally accepted
Sara Arghavani Sara Arghavani 1Younes Chorfi Younes Chorfi 1Mariela Segura Mariela Segura 1ACHRAF ADIB LESAUX ACHRAF ADIB LESAUX 2Marcio Carvalho Costa Marcio Carvalho Costa 1*
  • 1 Faculté de Médecine Vétérinaire, Université de Montréal, Montreal, Quebec, Canada
  • 2 Phileo Lesaffre Animal Care, Marcq-en-Barœul, France

The final, formatted version of the article will be published soon.

    The gut microbiota plays an important role in the health of dogs, but treatment with antibiotics causes marked dysbiosis. The objectives of this study were to evaluate the impact of yeast probiotic Saccharomyces cerevisiae supplementation on the fecal microbiota of dogs and its potential to prevent dysbiosis induced by antibiotics. Twenty healthy adult dogs were divided into a control and a yeast probiotic group receiving 1g/kg of S. cerevisiae (Actisaf®, Phileo by Lesaffre, Marcq-en-Baroeul, France) daily from D0 to D31. Both groups were given oral metronidazole from D11 to D17. Fecal swabs were collected on D0, 3, 11, 17, 20, 24, and 31 for microbiota analysis and blood on D0 and D24 for measurements of cytokines and cortisol. At D0, two distinct microbiota profiles comprised of dogs from both groups, control and probiotic, were identified. One profile had higher abundances of species related to stress and inflammation, and the other comprised species associated with good intestinal health. After three days of supplementation with yeast probiotic S. cerevisiae, all five dogs from the probiotic group having a stress-related microbiota (membership) shifted to a healthy microbiota. Metronidazole markedly changed the microbiota of both groups (p <0.001). Still, treated dogs had significantly different microbiota on D17 (end of antibiotics treatment). The dysbiosis was resolved in both groups by D24. TNF-α remarkably decreased from D0 to D24 (p=0.002) in the probiotic group, which also had lower levels than controls on D24 (p=0.040). There were no significant differences in the other measured cytokines. It was concluded that the use of yeast probiotic S. cerevisiae positively shifted the microbiota composition of healthy adult dogs carrying an abnormal microbial profile and that it has the potential to attenuate the dysbiosis caused by oral metronidazole.

    Keywords: Microbiota manipulation, yeast probiotics, Intestinal dysbiosis, Gut Microbiota, microbiome, antibiotics Font: Italic Formatted: Font: Italic Formatted: Font: Italic Deleted:, as revealed by the LEfSe analysis

    Received: 09 Jul 2024; Accepted: 06 Jan 2025.

    Copyright: © 2025 Arghavani, Chorfi, Segura, ADIB LESAUX and Costa. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Marcio Carvalho Costa, Faculté de Médecine Vétérinaire, Université de Montréal, Montreal, H3T 1J4, Quebec, Canada

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