Mengmeng Mi Yaqing Zheng Xin Fu Nan Bao Li Pan Yuan Zhao ^* Guixin Qin

• College of Animal Science and Technology, Jilin Agricultural University, Changchun, China

A small number of soybean allergens (including Glycinin (11S) and β-Conglycinin (7S)) in the commercially available corn-soybean meal diet can still cause allergy in some weaned piglets, which may be the result of the interaction of genetic, and nutrition, but the specific mechanism is still unclear. In this study, the results showed that the sensitization rate of soybean antigen in the corn-soybean meal diet was 21.74% and there was a gender difference with the sensitization rate of female pigs (31.34%) being higher than that of male pigs (13.23%). Moreover, the levels of inflammatory factors (IL-1β, IL-4, TNF-α) and antibodies (IgG, IgE, and specific IgG) in allergic piglets were significantly higher than those in non-allergic piglets (P < 0.05). Whole genome re-sequencing analysis revealed specific mutations in the exons and URT5 of trafficking protein particle complex subunit 2 (TRAPPC2), Pirin (PIR), complement factor properdin (CFP), and sosondowah ankyrin repeat domain family member D (SOWAHD) genes, which should be the reason for the significantly higher expression levels of related genes in the spleen of allergic piglets compared with non-allergic piglets (P < 0.05). Transcriptome analysis identified a large number of immune differential genes related to soybean antigen-induced intestinal immune response in piglets, such as IL17REL, CCL19, CD1E, and CD1.1, which were mainly involved in protein digestion pathway, cytokine interaction, and amino acid synthesis and degradation pathway. Transfection of CFP / TRAPPC2 / CCL19 siRNA could partially alleviate the injury of RAW264.7 cells or IPEC-J2 cells induced by β-Conglycinin. In the meantime, metabolomics results showed that soybean antigen affected the utilization and metabolism of intestinal nutrients in piglets, mainly the digestion and absorption of protein and the synthesis and metabolism of amino acids. Therefore, the individual differences in intestinal damage induced by soybean antigen protein in the corn-soybean meal diet are closely related to PIR, CFP, TRAPPC2, SOWAHD, and CCL19 genes. Soybean antigens affect the intestinal nutrient utilization and metabolism of piglets, which provides a scientific reference for the study of soybean antigen sensitization mechanisms, precision nutrition, disease prevention, and control of piglets, and also lays a foundation for human foodborne diseases.