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ORIGINAL RESEARCH article

Front. Vet. Sci.
Sec. Veterinary Regenerative Medicine
Volume 11 - 2024 | doi: 10.3389/fvets.2024.1517349
This article is part of the Research Topic Regenerative Potential of Natural Products in Veterinary Medicine View all articles

Impact of Eggshell Membrane on Metabolism and Cell Adhesion in Oxidatively Stressed Canine Chondrocytes

Provisionally accepted
Juraj Vozar Juraj Vozar 1Nikola Hdakova Nikola Hdakova 1Natalia Nosalova Natalia Nosalova 1Mykhailo Huniadi Mykhailo Huniadi 1Dana Marcincakova Dana Marcincakova 2Slavomir Hornak Slavomir Hornak 3Lubica Hornakova Lubica Hornakova 3Petra Majerova Petra Majerova 4Dasa Cizkova Dasa Cizkova 4,5*
  • 1 Centre of Experimental and Clinical Regenerative Medicine, Small Animal Clinic, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovakia
  • 2 Department of Pharmacology and Toxicology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovakia
  • 3 Small Animal Clinic, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovakia
  • 4 Institute of Neuroimmunology, Slovak Academy of Sciences, Bratislava, Slovakia
  • 5 University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovakia

The final, formatted version of the article will be published soon.

    Eggshell membrane (ESM) is a rich source of bioactive compounds, including proteins, peptides, and antioxidants, contributing to its potential therapeutic benefits. These natural antioxidants might help neutralize reactive oxygen species (ROS) and modulate inflammatory responses, which are often linked with chondrocyte damage in osteoarthritis. In this study, we investigated the functional effects of ESM proteins on H2O2-induced oxidative stress in a neonatal canine chondrocytes. The isolated neonatal chondrocytes demonstrated a high proliferation rate and increased glycosaminoglycan (GAG) production during cultivation. In addition, the expression of key cartilage markers, including collagen types II and IX, and aggrecan, confirmed the retention of the chondrocyte phenotype. Under in vitro conditions, post-treatment with ESM improved chondrocyte viability, indicating that ESM may have a reparative role in mitigating oxidative damage. This significant therapeutic potential was validated through XTT assays, which measured cell metabolic activity at 24 hours, and Real-time Cell Analysis (RTCA), providing continuous monitoring over 98 hours. In contrast, the preventive effects of ESM against stress were observed exclusively in the XTT analysis. By investigating these aspects, we provide insight into the potential of ESM proteins to protect chondrocytes from oxidative damage, particularly in cartilage repair and joint health. This study is one of the first to create a vital platform based on canine neonatal chondrocytes for monitoring dietary supplements designed to prevent or repair dog cartilage damage. Thus, the study offers a valuable contribution to understanding how ESM odstránil: Mitigates Oxidative

    Keywords: Eggshell membrane, Canine chondrocytes, Oxidative Stress, Osteoarthritis, prevention, treatment Normálny, Medzera Pred: 0 b, Za: 0 b

    Received: 25 Oct 2024; Accepted: 16 Dec 2024.

    Copyright: © 2024 Vozar, Hdakova, Nosalova, Huniadi, Marcincakova, Hornak, Hornakova, Majerova and Cizkova. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Dasa Cizkova, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovakia

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.