Skip to main content

ORIGINAL RESEARCH article

Front. Vet. Sci.
Sec. Animal Reproduction - Theriogenology
Volume 11 - 2024 | doi: 10.3389/fvets.2024.1514362
This article is part of the Research Topic Exploring 'Omic' Biomarkers in Animal Production and Reproduction View all 5 articles

Abnormalities in mitochondrial energy metabolism induced by cryopreservation negatively affect goat sperm motility

Provisionally accepted
Shengqin Zang Shengqin Zang 1Shuqi Zou Shuqi Zou 1Xiangyi Chen Xiangyi Chen 1Bo Pan Bo Pan 1Ao Ning Ao Ning 1Jianpeng Qin Jianpeng Qin 1Yaozong Wei Yaozong Wei 1Kunlin Du Kunlin Du 1Jiangfeng Ye Jiangfeng Ye 1Qiuxia Liang Qiuxia Liang 2Yi Fang Yi Fang 3Qiong la Qiong la 4Cirenla mu Cirenla mu 4Tianzeng Song Tianzeng Song 5*Guangbin Zhou Guangbin Zhou 1*
  • 1 College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China
  • 2 College of Life Science, Sichuan Agricultural University, Ya an, Sichuan Province, China
  • 3 College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin Province, China
  • 4 The Service Station of Agricultural and Animal, Husbandry Technical of Nyalam, Shigatse, Xizang, China
  • 5 Institute of Animal Science, Xizang Academy of Agricultural and Animal Husbandry Science, Lhasa, Xizang, China

The final, formatted version of the article will be published soon.

    The motility of sperm decreases following cryopreservation, which is closely associated with mitochondrial function. However, the alterations in mitochondrial metabolism after sperm freezing in goat remain unclear. This experiment aimed to investigate the impact of ultra-low temperature freezing on goat sperm's mitochondrial energy metabolism and its potential correlation with sperm motility.The results revealed that goat sperm exhibited mitochondrial vacuolization, reduced matrix density, significantly decreased levels of high-membrane potential mitochondria and adenosine triphosphate content, accompanied by a substantial increase in reactive oxygen species levels, ultimately leading to a significant decline in sperm viability. Further investigations unveiled that energy-related differential metabolites (capric acid, creatine, D-glucosamine-6-phosphate) as well as differential metabolites with antioxidant effects (saikosaponin a, probucol, cholesterol sulfate) were significantly down-regulated.Additionally, the activity of key rate-limiting enzymes involved in very long-chain fatty acid Cryopreservation negatively affects sperm metabolism biosynthesis and β-oxidation-specifically acetyl-CoA carboxylase, fatty acid synthase, and carnitine palmitoyltransferase I related to capric acid metabolism -was considerably reduced. Furthermore, supplementation of differential metabolite capric acid (500 μM) significantly enhanced the motility of frozen-thawed goat sperm. These findings indicated that the mitochondrial ultrastructure of goat sperm is damaged and energy metabolism becomes abnormal after cryopreservation, potentially affecting sperm viability. The addition of different metabolites such as capric acid to the freezing extender can alleviate the decrease in sperm motility induced by cryopreservation.

    Keywords: Goat sperm, Cryopreservation, Sperm Motility, Energy Metabolism, Metabolomics

    Received: 20 Oct 2024; Accepted: 13 Dec 2024.

    Copyright: © 2024 Zang, Zou, Chen, Pan, Ning, Qin, Wei, Du, Ye, Liang, Fang, la, mu, Song and Zhou. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Tianzeng Song, Institute of Animal Science, Xizang Academy of Agricultural and Animal Husbandry Science, Lhasa, Xizang, China
    Guangbin Zhou, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.