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ORIGINAL RESEARCH article

Front. Vet. Sci.
Sec. Veterinary Infectious Diseases
Volume 11 - 2024 | doi: 10.3389/fvets.2024.1510825
This article is part of the Research Topic Sequencing and Phylogenetic Analysis as a Tool in Molecular Epidemiology of Veterinary Infectious Diseases - Volume II View all 6 articles

Genetic structure and diversity of Mycoplasma hyopneumoniae based on a MLVA typing scheme

Provisionally accepted
Pablo Tamiozzo Pablo Tamiozzo 1Virginia García Virginia García 1Raúl E González-Ittig Raúl E González-Ittig 2Maria Pieters Maria Pieters 3*
  • 1 National University of Río Cuarto, Río Cuarto, Córdoba, Argentina
  • 2 National University of Cordoba, Córdoba, Córdoba, Argentina
  • 3 University of Minnesota Twin Cities, St. Paul, United States

The final, formatted version of the article will be published soon.

    Background: Several epidemiological studies have been carried out using different Multiple-Locus Variable-number tandem repeat Analysis (MLVA) for M. hyopneumoniae typing. However, a global perspective on the implications of the genetic diversity of this pathogen is lacking.Objective: This study aimed to determine and to analyze the genetic structure of M. hyopneumoniae based on the p97R1-p146R3 MLVA typing scheme and to characterize, analyze and compare MLVA types among countries where the information was publicly available.Methods: A set of 797 publicly available data of M. hyopneumoniae p97R1-p146R3 MLVA genetic types from six different countries were analyzed using Genalex 6.41 software to characterize loci polymorphism and using Structure 2.3.4 software in order to identify the genetic structure.Results: A total of 185 MLVA types were identified among the analyzed data. For the p97R1 and p146R3 loci, most of the molecular variation in M. hyopneumoniae populations was identified within countries. Three genetic clusters and their recombinations were identified globally.Conclusions: M. hyopneumoniae is a genetically diverse pathogen with limited clonality and three well-defined clusters and their combinations were identified in this investigation. The greatest genetic variation of M. hyopneumoniae was observed within countries.

    Keywords: Mycoplasma hyopneumoniae, Genetic structure, panmictic-clonality, MLVA, Porcine enzootic pneumonia, Admixed ancestry

    Received: 13 Oct 2024; Accepted: 06 Dec 2024.

    Copyright: © 2024 Tamiozzo, García, González-Ittig and Pieters. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Maria Pieters, University of Minnesota Twin Cities, St. Paul, United States

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