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ORIGINAL RESEARCH article

Front. Vet. Sci.
Sec. Animal Nutrition and Metabolism
Volume 11 - 2024 | doi: 10.3389/fvets.2024.1505854
This article is part of the Research Topic Mitigating Pathogen Load and AMR in Animals via Prebiotics and Probiotics View all 5 articles

Isolation and Evaluation of Multi-functional Properties of Lactic Acid Bacteria Strains Derived from Canine Milk

Provisionally accepted
Yunjiang Liu Yunjiang Liu 1Yueyan Zeng Yueyan Zeng 1Li Chen Li Chen 2Jialiang Xin Jialiang Xin 1Zhijun Zhong Zhijun Zhong 1Haifeng Liu Haifeng Liu 1Hualin Fu Hualin Fu 1ZIYAO ZHOU ZIYAO ZHOU 1Guangneng Peng Guangneng Peng 1*
  • 1 Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, China
  • 2 Key Laboratory of Animal Genetics and Breeding, State Ethnic Affairs Commission and Ministry of Education, Southwest Minzu University, Chengdu, Sichuan Province, China

The final, formatted version of the article will be published soon.

    Lactic acid bacteria (LAB) are Gram-positive bacteria that produce lactic acid during fermentation, with some strains enhancing host health by modulating the gut microbiota, boosting immune responses, and reducing inflammation. In this study, 6 LAB strains were isolated from two dog milk samples, and their probiotic properties were comprehensively evaluated. The evaluation included growth properties, stress resistance, antipathogen activity, adhesion activity, safety assessment, antioxidant capacity, and prebiotic metabolites assessment. In comparison to the control strain Lactobacillus rhamnosus LGG, all 6 LAB isolates exhibited favorable probiotic properties.Additionally, the results of the antioxidant tests indicated that these strains demonstrated high tolerance to 0.5 mmol/L H2O2 and exhibited significant scavenging abilities for the free radicals 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) and hydroxyl (OH -). Furthermore, the 6 LAB isolates were found to produce elevated concentrations of prebiotic metabolites, including exopolysaccharides (EPS), γ-aminobutyric acid (GABA), and bile salt hydrolase (BSH). This study presents a comprehensive analysis of LAB isolates derived from canine milk. These isolates exhibited multifunctional properties, with strain L221 performing the best overall, making it a promising candidate for probiotic use in dogs.

    Keywords: Lactic acid bacteria, canine milk, Adhesion, antioxidant capacity, metabolite

    Received: 03 Oct 2024; Accepted: 08 Nov 2024.

    Copyright: © 2024 Liu, Zeng, Chen, Xin, Zhong, Liu, Fu, ZHOU and Peng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Guangneng Peng, Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, Sichuan Province, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.